Strain identifier

BacDive ID: 6454

Type strain: No

Species: Lactobacillus delbrueckii subsp. lactis

Strain Designation: 313, Ld 5, Ld5

Strain history: CIP <- 1953, Lévy, Med. Univ., Paris, France <- ATCC, Lactobacillus leichmannii <- Tittsler: strain 313 <- E. McCoy: strain Ld5

NCBI tax ID(s): 29397 (subspecies)

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General

@ref: 8617

BacDive-ID: 6454

DSM-Number: 20355

keywords: genome sequence, Bacteria, anaerobe, mesophilic

description: Lactobacillus delbrueckii subsp. lactis 313 is an anaerobe, mesophilic bacterium of the family Lactobacillaceae.

NCBI tax id

  • NCBI tax id: 29397
  • Matching level: subspecies

strain history

@refhistory
8617<- Ellenrieder <- ATCC <- R.P. Tittsler, 313 (Lactobacillus leichmannii) <- McCoy, Ld 5 (Lactobacillus delbrueckii)
67770ATCC 7830 <-- R. P. Tittsler 313 <-- E. McCoy Ld5 (Lactobacillus delbrueckii).
118881CIP <- 1953, Lévy, Med. Univ., Paris, France <- ATCC, Lactobacillus leichmannii <- Tittsler: strain 313 <- E. McCoy: strain Ld5

doi: 10.13145/bacdive6454.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Bacilli
  • order: Lactobacillales
  • family: Lactobacillaceae
  • genus: Lactobacillus
  • species: Lactobacillus delbrueckii subsp. lactis
  • full scientific name: Lactobacillus delbrueckii subsp. lactis (Orla-Jensen 1919) Weiss et al. 1984

  • synonyms

    @refsynonym
    20215Thermobacterium lactis
    20215Lactobacillus lactis

@ref: 8617

domain: Bacteria

phylum: Firmicutes

class: Bacilli

order: Lactobacillales

family: Lactobacillaceae

genus: Lactobacillus

species: Lactobacillus delbrueckii subsp. lactis

full scientific name: Lactobacillus delbrueckii subsp. lactis (Orla-Jensen 1919) Weiss et al. 1984

strain designation: 313, Ld 5, Ld5

type strain: no

Morphology

cell morphology

@refmotilityconfidencegram staincell shape
69480no96.212
69480100positive
118881nopositiverod-shaped

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
8617PEDIOCOCCUS DAMNOSUS MEDIUM (DSMZ Medium 231)yeshttps://mediadive.dsmz.de/medium/231Name: PEDIOCOCCUS DAMNOSUS MEDIUM (DSMZ Medium 231) Composition: Glucose 20.0 g/l Casein peptone 10.0 g/l Meat extract 10.0 g/l Yeast extract 5.0 g/l Na-acetate 5.0 g/l K2HPO4 2.0 g/l (NH4)3 citrate 2.0 g/l Tween 80 1.0 g/l Cysteine hydrochloride 0.5 g/l MgSO4 x 7 H2O 0.2 g/l MnSO4 x H2O 0.05 g/l Distilled water
8617MRS MEDIUM (DSMZ Medium 11)yeshttps://mediadive.dsmz.de/medium/11Name: MRS MEDIUM (DSMZ Medium 11) Composition: Glucose 20.0 g/l Casein peptone 10.0 g/l Meat extract 10.0 g/l Na-acetate 5.0 g/l Yeast extract 5.0 g/l (NH4)3 citrate 2.0 g/l K2HPO4 2.0 g/l Tween 80 1.0 g/l MgSO4 x 7 H2O 0.2 g/l MnSO4 x H2O 0.05 g/l Distilled water
34200MEDIUM 40- for Lactobacillus and LeuconostocyesDistilled water make up to (1000.000 ml);Man Rogosa Sharp agar (68.000 g)
118881CIP Medium 40yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=40

culture temp

@refgrowthtypetemperaturerange
8617positivegrowth37mesophilic
34200positivegrowth37mesophilic
67770positivegrowth37mesophilic
118881positivegrowth30-45
118881nogrowth10psychrophilic
118881nogrowth15psychrophilic

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
8617anaerobe
8617microaerophile
118881facultative anaerobe

spore formation

  • @ref: 69480
  • spore formation: no
  • confidence: 99.995

murein

  • @ref: 8617
  • murein short key: A11.31
  • type: A4alpha L-Lys-D-Asp

observation

  • @ref: 67770
  • observation: Assay of Vitamin B12

metabolite utilization

@refmetaboliteutilization activitykind of utilization testedChebi-ID
68371Potassium 5-ketogluconate-builds acid from
68371Potassium 2-ketogluconate-builds acid from
68371gluconate-builds acid from24265
68371L-arabitol-builds acid from18403
68371D-arabitol-builds acid from18333
68371L-fucose-builds acid from18287
68371D-fucose-builds acid from28847
68371D-tagatose-builds acid from16443
68371D-lyxose-builds acid from62318
68371turanose-builds acid from32528
68371xylitol-builds acid from17151
68371glycogen-builds acid from28087
68371starch-builds acid from28017
68371raffinose-builds acid from16634
68371melezitose-builds acid from6731
68371inulin-builds acid from15443
68371sucrose+builds acid from17992
68371melibiose-builds acid from28053
68371esculin+builds acid from4853
68371methyl alpha-D-glucopyranoside-builds acid from320061
68371methyl alpha-D-mannoside-builds acid from43943
68371D-sorbitol-builds acid from17924
68371D-mannitol-builds acid from16899
68371myo-inositol-builds acid from17268
68371galactitol-builds acid from16813
68371L-rhamnose-builds acid from62345
68371L-sorbose-builds acid from17266
68371D-mannose+builds acid from16024
68371D-glucose+builds acid from17634
68371D-galactose-builds acid from12936
68371methyl beta-D-xylopyranoside-builds acid from74863
68371ribitol-builds acid from15963
68371L-xylose-builds acid from65328
68371D-xylose-builds acid from65327
68371D-ribose-builds acid from16988
68371L-arabinose-builds acid from30849
68371D-arabinose-builds acid from17108
68371erythritol-builds acid from17113
68371glycerol-builds acid from17754
118881nitrate-reduction17632
118881nitrite-reduction16301
118881nitrate+respiration17632

metabolite tests

  • @ref: 118881
  • Chebi-ID: 15688
  • metabolite: acetoin
  • voges-proskauer-test: -

enzymes

@refvalueactivityec
68382alpha-fucosidase-3.2.1.51
68382alpha-mannosidase-3.2.1.24
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382beta-glucosidase+3.2.1.21
68382alpha-glucosidase-3.2.1.20
68382beta-glucuronidase-3.2.1.31
68382beta-galactosidase+3.2.1.23
68382alpha-galactosidase-3.2.1.22
68382naphthol-AS-BI-phosphohydrolase+
68382acid phosphatase+3.1.3.2
68382alpha-chymotrypsin+3.4.21.1
68382trypsin-3.4.21.4
68382cystine arylamidase-3.4.11.3
68382valine arylamidase-
68382leucine arylamidase+3.4.11.1
68382lipase (C 14)-
68382esterase lipase (C 8)+
68382esterase (C 4)+
68382alkaline phosphatase-3.1.3.1
118881oxidase-
118881alcohol dehydrogenase+1.1.1.1
118881catalase-1.11.1.6
118881lysine decarboxylase-4.1.1.18
118881ornithine decarboxylase-4.1.1.17

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
118881--++-+---+++-+--+---

API 50CHac

@refQGLYERYDARALARARIBDXYLLXYLADOMDXGALGLUFRUMNESBERHADULINOMANSORMDMMDGNAGAMYARBESCSALCELMALLACMELSACTREINUMLZRAFAMDGLYGXLTGENTURLYXTAGDFUCLFUCDARLLARLGNT2KG5KG
8617-----------+++-----------+-----++-----------------
8617-----------+++--------++-+-+++-++------+----------
8617-----------++/-+-----------+-----++/------------------
118881----------+++---------+++++++-++------+----------

API biotype100

@refGLUFRUGALTREMNESBEMELSACRAFMTEMALLACLTEMbGaMaGaCELGENMbGuESCRIBARAXYLPLERHAFUCMLZDARLLARLXLTDULTAGGLYINOMANMTLTURSORADOHBGLYXERYMDG3MDGSATMUCLTATDTATMTATDMLTLMLTCATETATETTECITGRTGAT2KG5KGTRYNAGGNTPACPATpOBEQATGTEmOBEBATPPATCMTTGEBETPCEABTHINLATCAPCYTHISSUCFUMGREGYTAVTETNTTNGLNITA3OBUAPTGTTPRODALALALASERMNTPROPTYR2KT
118881---------------------------------------------------------------------------------------------------

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
86171Risk group (German classification)
1188811Risk group (French classification)

Sequence information

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Lactobacillus delbrueckii subsp. lactis KCTC 3035GCA_001888985completencbi29397
66792Lactobacillus delbrueckii subsp. lactis strain KCTC 303529397.16completepatric29397
66792Lactobacillus delbrueckii subsp. lactis strain NBRC 337629397.69wgspatric29397
66792Lactobacillus delbrueckii lactis KCTC 30352718218337completeimg29397
67770Lactobacillus delbrueckii subsp. lactis NBRC 3376GCA_006538625contigncbi29397
66792Lactobacillus delbrueckii subsp. lactis DSM 20355GCA_021134475contigncbi29397

GC content

  • @ref: 8617
  • GC-content: 50.5

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingno66no
flagellatedno97.946no
gram-positiveyes95.74no
anaerobicyes64.694no
aerobicno98.375yes
halophileyes78.926no
spore-formingno95.829no
glucose-utilyes93.478no
thermophileno98.606yes
motileno95.224no
glucose-fermentyes90.289no

External links

@ref: 8617

culture collection no.: DSM 20355, ATCC 7830, NCDO 8118, NCIB 302, JCM 1557, BCRC 11051, BCRC 80015, CCUG 19776, CECT 282, CIP 53.61, IAM 12066, IFO 3376, KCTC 1058, KCTC 3035, LMG 6401, NBRC 3376, NCDO 302, NCIMB 8118, PCM 2583

straininfo link

  • @ref: 75927
  • straininfo: 8913

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Pathogenicity12656203Characterization and bioavailability of vitamin B12-compounds from edible algae.Watanabe F, Takenaka S, Kittaka-Katsura H, Ebara S, Miyamoto EJ Nutr Sci Vitaminol (Tokyo)10.3177/jnsv.48.3252002Animals, Biological Availability, Eukaryota/*metabolism, Humans, Rats, Vitamin B 12/*isolation & purification/*pharmacokinetics, Vitamin B 12 Deficiency/*diet therapyEnzymology
Metabolism12949118Lactobacillus reuteri CRL1098 produces cobalamin.Taranto MP, Vera JL, Hugenholtz J, De Valdez GF, Sesma FJ Bacteriol10.1128/JB.185.18.5643-5647.20032003Alkyl and Aryl Transferases/genetics/metabolism, *Bacterial Proteins, Carbohydrate Metabolism, Chromatography, High Pressure Liquid/methods, Cyanides/chemistry, Fermentation, Genes, Bacterial, Glycerol/metabolism, Lactobacillus/genetics/*metabolism, Spectrophotometry, Ultraviolet, Vitamin B 12/analysis/*biosynthesis/isolation & purificationEnzymology
Enzymology22895746Optimisation of batch culture conditions for cell-envelope-associated proteinase production from Lactobacillus delbrueckii subsp. lactis ATCC(R) 7830.Agyei D, Potumarthi R, Danquah MKAppl Biochem Biotechnol10.1007/s12010-012-9839-92012*Batch Cell Culture Techniques, Cell Wall/*enzymology, Culture Media, Fermentation, Hydrogen-Ion Concentration, Lactobacillus delbrueckii/*enzymology, Peptide Hydrolases/*biosynthesis/chemistry, TemperatureCultivation
Pathogenicity35337633Determination of cobalamin and related compounds in foods.Watanabe F, Bito T, Koseki KVitam Horm10.1016/bs.vh.2022.01.0092022Chromatography, High Pressure Liquid/methods, *Food Analysis/methods, Humans, *Vitamin B 12

Reference

@idauthorscataloguedoi/urltitle
8617Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 20355)https://www.dsmz.de/collection/catalogue/details/culture/DSM-20355
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
34200Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/15944
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68371Automatically annotated from API 50CH acid
68382Automatically annotated from API zym
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
75927Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID8913.1StrainInfo: A central database for resolving microbial strain identifiers
118881Curators of the CIPCollection of Institut Pasteur (CIP 53.61)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%2053.61