Strain identifier

BacDive ID: 6453

Type strain: No

Species: Lactobacillus leichmannii

Strain Designation: 326, F 59

Strain history: CIP <- 1954, ATCC <- R.P. Tittsler: strain 326 <- E.B. Fred: strain F5, Lactobacillus leichmannii

NCBI tax ID(s): 28039 (species), 29397 (subspecies)

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General

@ref: 8504

BacDive-ID: 6453

DSM-Number: 20076

keywords: genome sequence, 16S sequence, Bacteria, anaerobe, Gram-positive, rod-shaped

description: Lactobacillus leichmannii 326 is an anaerobe, Gram-positive, rod-shaped bacterium of the family Lactobacillaceae.

NCBI tax id

NCBI tax idMatching level
29397subspecies
28039species

strain history

@refhistory
8504<- ATCC <- R.P. Tittsler, 326 <- E.B. Fred, F 59
67770ATCC 4797 <-- R. P. Tittsler 326 <-- E. B. Fred F59.
118882CIP <- 1954, ATCC <- R.P. Tittsler: strain 326 <- E.B. Fred: strain F5, Lactobacillus leichmannii

doi: 10.13145/bacdive6453.20240916.9.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Bacilli
  • order: Lactobacillales
  • family: Lactobacillaceae
  • genus: Lactobacillus
  • species: Lactobacillus leichmannii
  • full scientific name: Lactobacillus leichmannii (Henneberg 1903) Bergey et al. 1923 (Approved Lists 1980)

  • synonyms

    • @ref: 20215
    • synonym: Bacillus leichmanni

@ref: 8504

domain: Bacteria

phylum: Firmicutes

class: Bacilli

order: Lactobacillales

family: Lactobacillaceae

genus: Lactobacillus

species: Lactobacillus delbrueckii subsp. lactis

full scientific name: Lactobacillus delbrueckii subsp. lactis (Orla-Jensen 1919) Weiss et al. 1984

strain designation: 326, F 59

type strain: no

Morphology

cell morphology

@refgram staincell shapemotilityconfidence
118882positiverod-shapedno
69480positive94.248
69480no95.5

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
8504MRS MEDIUM (DSMZ Medium 11)yeshttps://mediadive.dsmz.de/medium/11Name: MRS MEDIUM (DSMZ Medium 11) Composition: Glucose 20.0 g/l Casein peptone 10.0 g/l Meat extract 10.0 g/l Na-acetate 5.0 g/l Yeast extract 5.0 g/l (NH4)3 citrate 2.0 g/l K2HPO4 2.0 g/l Tween 80 1.0 g/l MgSO4 x 7 H2O 0.2 g/l MnSO4 x H2O 0.05 g/l Distilled water
35815MEDIUM 40- for Lactobacillus and LeuconostocyesDistilled water make up to (1000.000 ml);Man Rogosa Sharp agar (68.000 g)
118882CIP Medium 40yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=40

culture temp

@refgrowthtypetemperature
8504positivegrowth37
35815positivegrowth37
67770positivegrowth37
118882positivegrowth22-45
118882nogrowth10
118882nogrowth15

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
8504anaerobe
8504microaerophile
118882facultative anaerobe

murein

  • @ref: 8504
  • murein short key: A11.31
  • type: A4alpha L-Lys-D-Asp

observation

  • @ref: 67770
  • observation: Assay of Vitamin B12

metabolite utilization

@refmetaboliteutilization activitykind of utilization testedChebi-ID
68371Potassium 5-ketogluconate-builds acid from
68371Potassium 2-ketogluconate-builds acid from
68371gluconate-builds acid from24265
68371L-arabitol-builds acid from18403
68371D-arabitol-builds acid from18333
68371L-fucose-builds acid from18287
68371D-fucose-builds acid from28847
68371D-tagatose-builds acid from16443
68371D-lyxose-builds acid from62318
68371turanose-builds acid from32528
68371xylitol-builds acid from17151
68371glycogen-builds acid from28087
68371starch-builds acid from28017
68371raffinose-builds acid from16634
68371melezitose-builds acid from6731
68371inulin-builds acid from15443
68371trehalose+builds acid from27082
68371sucrose+builds acid from17992
68371melibiose-builds acid from28053
68371lactose-builds acid from17716
68371maltose+builds acid from17306
68371esculin+builds acid from4853
68371methyl alpha-D-glucopyranoside-builds acid from320061
68371methyl alpha-D-mannoside-builds acid from43943
68371D-sorbitol-builds acid from17924
68371D-mannitol-builds acid from16899
68371myo-inositol-builds acid from17268
68371galactitol-builds acid from16813
68371L-rhamnose-builds acid from62345
68371L-sorbose-builds acid from17266
68371D-fructose+builds acid from15824
68371D-glucose+builds acid from17634
68371methyl beta-D-xylopyranoside-builds acid from74863
68371ribitol-builds acid from15963
68371L-xylose-builds acid from65328
68371D-xylose-builds acid from65327
68371D-ribose-builds acid from16988
68371L-arabinose-builds acid from30849
68371D-arabinose-builds acid from17108
68371erythritol-builds acid from17113
68371glycerol-builds acid from17754
118882nitrate-reduction17632
118882nitrite-reduction16301
118882nitrate+respiration17632
68379nitrate-reduction17632
68379esculin+hydrolysis4853
68379urea-hydrolysis16199
68379gelatin-hydrolysis5291
68379D-ribose-fermentation16988
68379D-xylose-fermentation65327
68379D-mannitol-fermentation16899
68379maltose+fermentation17306
68379lactose+fermentation17716
68379sucrose+fermentation17992
68379glycogen-fermentation28087

metabolite tests

@refChebi-IDmetabolitevoges-proskauer-testmethylred-test
11888215688acetoin-
11888217234glucose-

enzymes

@refvalueactivityec
68382alpha-fucosidase-3.2.1.51
68382alpha-mannosidase-3.2.1.24
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382beta-glucosidase+3.2.1.21
68382alpha-glucosidase-3.2.1.20
68382beta-glucuronidase-3.2.1.31
68382beta-galactosidase-3.2.1.23
68382alpha-galactosidase-3.2.1.22
68382naphthol-AS-BI-phosphohydrolase+
68382acid phosphatase+3.1.3.2
68382alpha-chymotrypsin+3.4.21.1
68382trypsin-3.4.21.4
68382cystine arylamidase-3.4.11.3
68382valine arylamidase-
68382leucine arylamidase+3.4.11.1
68382lipase (C 14)-
68382esterase lipase (C 8)-
68382esterase (C 4)+
68382alkaline phosphatase-3.1.3.1
68379catalase-1.11.1.6
68379gelatinase-
68379urease-3.5.1.5
68379beta-glucosidase+3.2.1.21
68379N-acetyl-beta-glucosaminidase-3.2.1.52
68379alpha-glucosidase-3.2.1.20
68379beta-galactosidase+3.2.1.23
68379beta-glucuronidase-3.2.1.31
68379alkaline phosphatase-3.1.3.1
68379pyrrolidonyl arylamidase-3.4.19.3
68379pyrazinamidase-3.5.1.B15
118882oxidase-
118882alcohol dehydrogenase-1.1.1.1
118882catalase-1.11.1.6
118882lysine decarboxylase-4.1.1.18
118882ornithine decarboxylase-4.1.1.17

API coryne

@refNITPYZPYRAPALbeta GURbeta GALalpha GLUbeta NAGESCUREGELControlRIBXYLMANMALLACSACGLYGCAT
47415-----+--+------+++--

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
118882--+--+---+++----+---

API 50CHac

@refQGLYERYDARALARARIBDXYLLXYLADOMDXGALGLUFRUMNESBERHADULINOMANSORMDMMDGNAGAMYARBESCSALCELMALLACMELSACTREINUMLZRAFAMDGLYGXLTGENTURLYXTAGDFUCLFUCDARLLARLGNT2KG5KG
8504-----------+++/-----------+/-++/--+--++-----------------
8504-----------+++----------+++-+--++-----------------
118882---------+++---------+++++++--++------+----------

Isolation, sampling and environmental information

taxonmaps

  • @ref: 69479
  • File name: preview.99_226.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_46;96_161;97_172;98_193;99_226&stattab=map
  • Last taxonomy: Lactobacillus delbrueckii
  • 16S sequence: JX986966
  • Sequence Identity:
  • Total samples: 60658
  • soil counts: 2030
  • aquatic counts: 6667
  • animal counts: 50988
  • plant counts: 973

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
85041Risk group (German classification)
1188821Risk group (French classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Lactobacillus delbrueckii subsp. lactis genes for 16S rRNA, 16S-23S intergenic spacer region, 23S rRNA, partial and complete sequence, strain:ATCC 4797AB092635690nuccore29397
20218Lactobacillus delbrueckii ATCC 4797 16S ribosomal RNA gene, partial sequenceAF429504502nuccore1584
20218Lactobacillus delbrueckii strain ATCC 4797 16S ribosomal RNA gene, partial sequence; 16S-23S intergenic spacer, complete sequence; and 23S ribosomal RNA gene, partial sequenceAF429591538nuccore1584
67770Lactobacillus leichmannii 16S ribosomal RNA gene, partial sequenceJX9869661479nuccore28039

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Lactobacillus delbrueckii subsp. lactis NCIMB 8183GCA_021134395scaffoldncbi29397
66792Lactobacillus delbrueckii subsp. lactis DSM 20076GCA_021134495scaffoldncbi29397
66792Lactobacillus leichmannii JCM 1148GCA_024701915contigncbi28039

GC content

  • @ref: 8504
  • GC-content: 50.8

Genome-based predictions

predictions

@reftraitmodeldescriptionpredictionconfidencetraining_data
69480gram-positivegram-positivePositive reaction to Gram-stainingyes94.248no
69480anaerobicanaerobicAbility to grow under anoxygenic conditions (including facultative anaerobes)no78.009no
69480aerobicaerobicAbility to grow under oxygenic conditions (including facultative aerobes)no97.735no
69480spore-formingspore-formingAbility to form endo- or exosporesno88.832no
69480thermophilicthermophileAbility to grow at temperatures above or equal to 45°Cno95yes
69480flagellatedmotile2+Ability to perform flagellated movementno95.5no

External links

@ref: 8504

culture collection no.: DSM 20076, ATCC 4797, CCM 2344, NCDO 299, NCIB 7854, CCUG 21454, ATCC 7831, JCM 1148, BCRC 10699, CECT 287, CIP 54.3, IAM 1196, IFO 3073, LMG 13136, LMG 6891, NBRC 3073, NCCB 49006, NCFB 299, NCFB 301, NCIMB 7854, NCIMB 8117, NCIMB 8183, NRRL B-1924, NRRL B-735

straininfo link

  • @ref: 75926
  • straininfo: 3283

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Metabolism1539976Jenseniin G, a heat-stable bacteriocin produced by Propionibacterium jensenii P126.Grinstead DA, Barefoot SFAppl Environ Microbiol10.1128/aem.58.1.215-220.19921992Bacteriocins/*biosynthesis/pharmacology, Catalase/metabolism, Hot Temperature, Kinetics, Microbial Sensitivity Tests, Propionibacterium/*metabolism, TemperatureStress
Phylogeny3938519[Production of vitamin B12 by fermentation].Ogultekin R, Oner MMikrobiyol Bul1985Biological Assay, Fermentation, Lactobacillus/growth & development, Species Specificity, Streptomyces/*metabolism, Streptomyces griseus/*metabolism, Vitamin B 12/*biosynthesisMetabolism
Phylogeny7986029Identification and purification of a protein that induces production of the Lactobacillus acidophilus bacteriocin lactacin B.Barefoot SF, Chen YR, Hughes TA, Bodine AB, Shearer MY, Hughes MDAppl Environ Microbiol10.1128/aem.60.10.3522-3528.19941994Amino Acid Sequence, Bacterial Proteins/genetics/isolation & purification/*metabolism, Bacteriocins/*biosynthesis, Hydrogen-Ion Concentration, Lactobacillus/genetics/metabolism, Lactobacillus acidophilus/*metabolism, Molecular Sequence Data, Species SpecificityMetabolism
Cultivation11169042A rapid procedure for isolating chromosomal DNA from Lactobacillus species and other Gram-positive bacteria.Ulrich RL, Hughes TALett Appl Microbiol10.1046/j.1472-765x.2001.00866.x2001Chromosomes, Bacterial/*genetics, Culture Media, DNA, Bacterial/*isolation & purification, Gene Library, Glycine/chemistry, Gram-Positive Bacteria/*genetics/growth & development, Lactobacillus/*genetics/growth & development, Muramidase/metabolism, Polymerase Chain Reaction/methods, Restriction MappingEnzymology
Biotechnology11403125Inactivation of Escherichia coli O1 57:H7, Listeria monocytogenes, and Lactobacillus leichmannii by combinations of ozone and pulsed electric field.Unal R, Kim JG, Yousef AEJ Food Prot10.4315/0362-028x-64.6.7772001Colony Count, Microbial, *Electricity, Escherichia coli O157/*growth & development, Food Microbiology, Food Preservation/*methods, Lactobacillus/*growth & development, Listeria monocytogenes/*growth & development, Ozone/*pharmacology
Enzymology11851815Cloning and expression analysis of the 28 kDa protein from Lactobacillus delbrueckii subsp. lactis ATCC 4797 hypothesized to influence lactacin B production.Ulrich RL, Hughes TAJ Appl Microbiol10.1046/j.1365-2672.2001.01480.x2001Bacterial Proteins/genetics/isolation & purification/*metabolism, Bacteriocins/*biosynthesis, *Cloning, Molecular, Electrophoresis, Polyacrylamide Gel, Lactobacillus/genetics/*metabolism, Molecular Sequence Data, Physical Chromosome Mapping, Sequence Analysis, DNAMetabolism
Biotechnology24563313Homo-fermentative production of D-lactic acid by Lactobacillus sp. employing casein whey permeate as a raw feed-stock.Prasad S, Srikanth K, Limaye AM, Sivaprakasam SBiotechnol Lett10.1007/s10529-014-1482-92014Bioreactors, Culture Media/chemistry, Fermentation, Food Handling, Industrial Waste, Lactic Acid/*metabolism, Lactobacillus/*growth & development/*metabolismMetabolism
Pathogenicity25036875Protective role of probiotic lactic acid bacteria against dietary fumonisin B1-induced toxicity and DNA-fragmentation in sprague-dawley rats.Khalil AA, Abou-Gabal AE, Abdellatef AA, Khalid AEPrep Biochem Biotechnol10.1080/10826068.2014.9409692015Administration, Oral, Animals, Chemical and Drug Induced Liver Injury/genetics/*microbiology/*prevention & control, DNA Damage/drug effects/*genetics, DNA Fragmentation/*drug effects, Food Contamination, Fumonisins/administration & dosage/*toxicity, Lactobacillus/*physiology, Male, Probiotics/*therapeutic use, Rats, Rats, Sprague-Dawley, Teratogens/toxicity, Treatment Outcome

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
8504Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 20076)https://www.dsmz.de/collection/catalogue/details/culture/DSM-20076
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
35815Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/17400
47415Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 21454)https://www.ccug.se/strain?id=21454
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68371Automatically annotated from API 50CH acid
68379Automatically annotated from API Coryne
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
75926Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID3283.1StrainInfo: A central database for resolving microbial strain identifiers
118882Curators of the CIPCollection of Institut Pasteur (CIP 54.3)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%2054.3