Strain identifier

BacDive ID: 6451

Type strain: Yes

Species: Lactobacillus delbrueckii subsp. lactis

Strain Designation: L 110

Strain history: CIP <- 1984, DSM <- ATCC <- P.A. Hansen: strain L110, Lactobacillus lactis <- A. Snog-Kjaer <- S. Orla-Jensen: strain 10, Thermobacterium lactis

NCBI tax ID(s): 888027 (strain), 29397 (subspecies)

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General

@ref: 8500

BacDive-ID: 6451

DSM-Number: 20072

keywords: genome sequence, 16S sequence, Bacteria, anaerobe, mesophilic

description: Lactobacillus delbrueckii subsp. lactis L 110 is an anaerobe, mesophilic bacterium that was isolated from emmental cheese.

NCBI tax id

NCBI tax idMatching level
888027strain
29397subspecies

strain history

@refhistory
8500<- ATCC <- P.A. Hansen, L 110 <- A. Snog-Kjaer (S. Orla-Jensen Thermobacterium lactis No. 10)
67770ATCC 12315 <-- P. A. Hansen L110 <-- A. Song-Kjaer (S. Orla-Jensen, "Thermobacterium lactis" No. 10).
118880CIP <- 1984, DSM <- ATCC <- P.A. Hansen: strain L110, Lactobacillus lactis <- A. Snog-Kjaer <- S. Orla-Jensen: strain 10, Thermobacterium lactis

doi: 10.13145/bacdive6451.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Bacilli
  • order: Lactobacillales
  • family: Lactobacillaceae
  • genus: Lactobacillus
  • species: Lactobacillus delbrueckii subsp. lactis
  • full scientific name: Lactobacillus delbrueckii subsp. lactis (Orla-Jensen 1919) Weiss et al. 1984

  • synonyms

    @refsynonym
    20215Thermobacterium lactis
    20215Lactobacillus lactis

@ref: 8500

domain: Bacteria

phylum: Firmicutes

class: Bacilli

order: Lactobacillales

family: Lactobacillaceae

genus: Lactobacillus

species: Lactobacillus delbrueckii subsp. lactis

full scientific name: Lactobacillus delbrueckii subsp. lactis (Orla-Jensen 1919) Weiss et al. 1984

strain designation: L 110

type strain: yes

Morphology

cell morphology

@refmotilityconfidencegram staincell shape
69480no96.989
69480100positive
118880nopositiverod-shaped

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
8500MRS MEDIUM (DSMZ Medium 11)yeshttps://mediadive.dsmz.de/medium/11Name: MRS MEDIUM (DSMZ Medium 11) Composition: Glucose 20.0 g/l Casein peptone 10.0 g/l Meat extract 10.0 g/l Na-acetate 5.0 g/l Yeast extract 5.0 g/l (NH4)3 citrate 2.0 g/l K2HPO4 2.0 g/l Tween 80 1.0 g/l MgSO4 x 7 H2O 0.2 g/l MnSO4 x H2O 0.05 g/l Distilled water
40284MEDIUM 40- for Lactobacillus and LeuconostocyesDistilled water make up to (1000.000 ml);Man Rogosa Sharp agar (68.000 g)
118880CIP Medium 40yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=40

culture temp

@refgrowthtypetemperaturerange
8500positivegrowth37mesophilic
40284positivegrowth37mesophilic
50609positivegrowth37mesophilic
67770positivegrowth37mesophilic
118880positivegrowth22-45
118880nogrowth10psychrophilic
118880nogrowth15psychrophilic

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
8500anaerobe
8500microaerophile
50609anaerobe
50609microaerophile
118880facultative anaerobe

spore formation

@refspore formationconfidence
69481no100
69480no99.992

compound production

  • @ref: 67770
  • compound: Lacticin

murein

  • @ref: 8500
  • murein short key: A11.31
  • type: A4alpha L-Lys-D-Asp

metabolite utilization

@refmetaboliteutilization activitykind of utilization testedChebi-ID
68371Potassium 5-ketogluconate-builds acid from
68371Potassium 2-ketogluconate-builds acid from
68371gluconate-builds acid from24265
68371L-arabitol-builds acid from18403
68371D-arabitol-builds acid from18333
68371L-fucose-builds acid from18287
68371D-fucose-builds acid from28847
68371D-tagatose-builds acid from16443
68371D-lyxose-builds acid from62318
68371turanose-builds acid from32528
68371gentiobiose-builds acid from28066
68371xylitol-builds acid from17151
68371glycogen-builds acid from28087
68371starch-builds acid from28017
68371melezitose-builds acid from6731
68371inulin-builds acid from15443
68371melibiose-builds acid from28053
68371cellobiose-builds acid from17057
68371arbutin-builds acid from18305
68371amygdalin-builds acid from27613
68371methyl alpha-D-glucopyranoside-builds acid from320061
68371methyl alpha-D-mannoside-builds acid from43943
68371D-sorbitol-builds acid from17924
68371D-mannitol-builds acid from16899
68371myo-inositol-builds acid from17268
68371galactitol-builds acid from16813
68371L-rhamnose-builds acid from62345
68371L-sorbose-builds acid from17266
68371D-galactose-builds acid from12936
68371methyl beta-D-xylopyranoside-builds acid from74863
68371ribitol-builds acid from15963
68371L-xylose-builds acid from65328
68371D-xylose-builds acid from65327
68371D-ribose-builds acid from16988
68371L-arabinose-builds acid from30849
68371D-arabinose-builds acid from17108
68371erythritol-builds acid from17113
68371glycerol-builds acid from17754
118880nitrate-reduction17632
118880nitrite-reduction16301
118880nitrate+respiration17632

metabolite production

  • @ref: 118880
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

metabolite tests

  • @ref: 118880
  • Chebi-ID: 15688
  • metabolite: acetoin
  • voges-proskauer-test: -

enzymes

@refvalueactivityec
68382alpha-fucosidase-3.2.1.51
68382alpha-mannosidase-3.2.1.24
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382beta-glucosidase-3.2.1.21
68382alpha-glucosidase-3.2.1.20
68382beta-glucuronidase-3.2.1.31
68382beta-galactosidase+3.2.1.23
68382alpha-galactosidase-3.2.1.22
68382naphthol-AS-BI-phosphohydrolase+
68382acid phosphatase+3.1.3.2
68382alpha-chymotrypsin-3.4.21.1
68382trypsin-3.4.21.4
68382cystine arylamidase-3.4.11.3
68382valine arylamidase-
68382leucine arylamidase+3.4.11.1
68382lipase (C 14)-
68382esterase lipase (C 8)-
68382esterase (C 4)-
68382alkaline phosphatase-3.1.3.1
118880oxidase-
118880alcohol dehydrogenase-1.1.1.1
118880catalase-1.11.1.6
118880lysine decarboxylase-4.1.1.18
118880ornithine decarboxylase-4.1.1.17
118880urease-3.5.1.5

fatty acid profile

  • @reffatty acidpercentageECL
    50609C14:07.614
    50609C16:017.716
    50609C16:1 ω7c18.515.819
    50609C16:1 ω7c/C15:0 ISO 2OH5.115.85
    50609C18:1 ω7c /12t/9t4.317.824
    50609C18:1 ω9c20.617.769
    50609C19:0 CYCLO ω9c26.318.87
  • type of FA analysis: whole cell analysis
  • method/protocol: CCUG
  • @reffatty acidpercentageECL
    50609C14:03.714
    50609C16:031.416
    50609C18:03.918
    50609C16:1 ω7c7.415.819
    50609C16:1 ω7c/C15:0 ISO 2OH1415.85
    50609C17:0 CYCLO3.216.888
    50609C18:1 ω9c5.117.769
    50609C18:2 ω6,9c/C18:0 ANTE2017.724
    50609C19:0 CYCLO ω8c6.218.9
    50609C19:0 CYCLO ω9c5.218.87
  • type of FA analysis: whole cell analysis
  • method/protocol: CCUG

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
118880-----+----++-+------

API 50CHac

@refQGLYERYDARALARARIBDXYLLXYLADOMDXGALGLUFRUMNESBERHADULINOMANSORMDMMDGNAGAMYARBESCSALCELMALLACMELSACTREINUMLZRAFAMDGLYGXLTGENTURLYXTAGDFUCLFUCDARLLARLGNT2KG5KG
8500-----------+++--------+------+-++-----------------
8500-----------+++--------+------+-++-----------------
8500-----------+++--------+---+/--+/-+-++--+--------------
8500-----------+++--------+--++-++-++--+--------------
118880----------+/-+/-+/---------+/------+/-+/--+/-+/------------------

Isolation, sampling and environmental information

isolation

@refsample type
8500emmental cheese
50609Cheese,Swiss Emmenthal
67770Emmental (Swiss) cheese
118880Food, Cheese, Emmental Swiss

isolation source categories

  • Cat1: #Engineered
  • Cat2: #Food production
  • Cat3: #Dairy product

taxonmaps

  • @ref: 69479
  • File name: preview.99_226.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_46;96_161;97_172;98_193;99_226&stattab=map
  • Last taxonomy: Lactobacillus delbrueckii
  • 16S sequence: LC071822
  • Sequence Identity:
  • Total samples: 60658
  • soil counts: 2030
  • aquatic counts: 6667
  • animal counts: 50988
  • plant counts: 973

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
85001Risk group (German classification)
1188801Risk group (French classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Lactobacillus delbrueckii subsp. lactis ATCC 12315 16S ribosomal RNA gene, partial sequenceAF429505502ena888027
20218Lactobacillus delbrueckii subsp. lactis strain ATCC 12315 16S ribosomal RNA gene, partial sequence; 16S-23S intergenic spacer, complete sequence; and 23S ribosomal RNA gene, partial sequenceAF429592536ena888027
20218Lactobacillus delbrueckii subsp. lactis partial 16S rRNA gene, strain ATCC 12315AJ414695535ena888027
20218Lactobacillus delbrueckii subsp. lactis 16S ribosomal RNA gene, partial sequenceAY0501731505ena888027
20218Lactobacillus delbrueckii subsp. lactis partial 16S rRNA gene, type strain DSM 20072TFR6831031445ena29397
20218Lactobacillus lactis 16S ribosomal RNAM588231569ena29397
20218Lactobacillus delbrueckii gene, 16S-23S intergenic spacer region and 23S rRNA gene 5' flanking region, partial sequenceAB035484672ena888027
20218Lactobacillus delbrueckii subsp. lactis gene for 16S rRNA, partial sequence, strain: JCM 1248AB289094663ena888027
20218Lactobacillus delbrueckii gene for 16S ribosomal RNA, partial sequenceD31687192ena888027
20218Lactobacillus delbrueckii subsp. lactis strain LMG 7942T 16S ribosomal RNA gene, partial sequenceJQ580992980ena29397
20218Lactobacillus delbrueckii subsp. lactis gene for 16S rRNA, partial sequence, strain: NBRC 102622AB6818881489ena29397
67770Lactobacillus delbrueckii subsp. lactis gene for 16S ribosomal RNA, partial sequence, strain: JCM 1248LC0718221495ena888027

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Lactobacillus delbrueckii subsp. lactis DSM 20072GCA_002278095completencbi888027
66792Lactobacillus delbrueckii subsp. lactis DSM 20072888027.3wgspatric888027
66792Lactobacillus delbrueckii subsp. lactis DSM 20072888027.6wgspatric888027
66792Lactobacillus delbrueckii subsp. lactis DSM 20072888027.8completepatric888027
66792Lactobacillus delbrueckii subsp. lactis DSM 20072 strain DSM 20072888027.7wgspatric888027
66792Lactobacillus delbrueckii lactis DSM 200722916482346draftimg888027
66792Lactobacillus delbrueckii lactis DSM 200722534682072draftimg888027
66792Lactobacillus delbrueckii lactis DSM 200722663763110draftimg888027
66792Lactobacillus delbrueckii lactis DSM 200722879984508completeimg888027
67770Lactobacillus delbrueckii subsp. lactis DSM 20072GCA_000192165scaffoldncbi888027
67770Lactobacillus delbrueckii subsp. lactis DSM 20072GCA_001434635scaffoldncbi888027
67770Lactobacillus delbrueckii subsp. lactis DSM 20072GCA_002017855contigncbi888027

GC content

@refGC-contentmethod
850050.2
6777049.9genome sequence analysis

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingno100no
flagellatedno97.922no
gram-positiveyes95.408no
anaerobicyes60.941no
aerobicno98.593yes
halophileyes84.624no
spore-formingno96.595no
glucose-utilyes93.597no
thermophileno98.61no
motileno95.256no
glucose-fermentyes91.362no

External links

@ref: 8500

culture collection no.: DSM 20072, ATCC 12315, NCDO 1438, CCUG 31454, IAM 12476, JCM 1248, BCRC 12256, CGMCC 1.2132, CGMCC 1.2625, CIP 101028, KACC 12417, KCTC 3636, LMG 7942, NBIMCC 1012, NBRC 102622, NCFB 1438, NRIC 1683, NRRL B-4525, VTT E-93488

straininfo link

  • @ref: 75924
  • straininfo: 3282

literature

  • topic: Metabolism
  • Pubmed-ID: 21165615
  • title: Continuous D-lactic acid production by a novel thermotolerant Lactobacillus delbrueckii subsp. lactis QU 41.
  • authors: Tashiro Y, Kaneko W, Sun Y, Shibata K, Inokuma K, Zendo T, Sonomoto K
  • journal: Appl Microbiol Biotechnol
  • DOI: 10.1007/s00253-010-3011-7
  • year: 2010
  • mesh: Culture Media/chemistry, DNA, Bacterial/chemistry/genetics, DNA, Ribosomal/chemistry/genetics, Glucose/metabolism, *Hot Temperature, Hydrogen-Ion Concentration, Lactic Acid/*metabolism, Lactobacillus delbrueckii/growth & development/*metabolism/*radiation effects, Molecular Sequence Data, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA
  • topic2: Stress

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
8500Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 20072)https://www.dsmz.de/collection/catalogue/details/culture/DSM-20072
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
40284Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/12558
50609Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 31454)https://www.ccug.se/strain?id=31454
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68371Automatically annotated from API 50CH acid
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch10.21203/rs.3.rs-2527258/v1Predictions based on the model GenomeNet Sporulation v. 1
75924Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID3282.1StrainInfo: A central database for resolving microbial strain identifiers
118880Curators of the CIPCollection of Institut Pasteur (CIP 101028)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20101028