Strain identifier

BacDive ID: 6450

Type strain: Yes

Species: Lactobacillus delbrueckii

Strain Designation: 730, Calvert, Calbert

Strain history: CIP <- 1957, NCIB <- 1950, ATCC <- R.P. Tittsler: strain 730/ strain Calbert <- M. Rogosa

NCBI tax ID(s): 1423823 (strain), 1584 (species)

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General

@ref: 8502

BacDive-ID: 6450

DSM-Number: 20074

keywords: genome sequence, 16S sequence, Bacteria, anaerobe, mesophilic

description: Lactobacillus delbrueckii 730 is an anaerobe, mesophilic bacterium that was isolated from sour grain mash.

NCBI tax id

NCBI tax idMatching level
1423823strain
1584species

strain history

@refhistory
8502<- ATCC <- R.P. Tittsler, 730 strain Calvert <- M. Rogosa
67770T. Mitsuoka S1-42 <-- ISL <-- ATCC 9649 <-- R. P. Tittsler 730 (strain Calvert) <-- M. Rogosa.
118879CIP <- 1957, NCIB <- 1950, ATCC <- R.P. Tittsler: strain 730/ strain Calbert <- M. Rogosa

doi: 10.13145/bacdive6450.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Bacilli
  • order: Lactobacillales
  • family: Lactobacillaceae
  • genus: Lactobacillus
  • species: Lactobacillus delbrueckii
  • full scientific name: Lactobacillus delbrueckii (Leichmann 1896) Beijerinck 1901 (Approved Lists 1980)

  • synonyms

    • @ref: 20215
    • synonym: Bacillus delbruecki

@ref: 8502

domain: Bacteria

phylum: Firmicutes

class: Bacilli

order: Lactobacillales

family: Lactobacillaceae

genus: Lactobacillus

species: Lactobacillus delbrueckii subsp. delbrueckii

full scientific name: Lactobacillus delbrueckii subsp. delbrueckii (Leichmann 1896) Weiss et al. 1984

strain designation: 730, Calvert, Calbert

type strain: yes

Morphology

cell morphology

@refmotilityconfidencegram staincell shape
69480no96.291
69480100positive
118879nopositiverod-shaped

Culture and growth conditions

culture medium

@refnamegrowthcompositionlink
40059MEDIUM 40- for Lactobacillus and LeuconostocyesDistilled water make up to (1000.000 ml);Man Rogosa Sharp agar (68.000 g)
8502MRS MEDIUM (DSMZ Medium 11)yesName: MRS MEDIUM (DSMZ Medium 11) Composition: Glucose 20.0 g/l Casein peptone 10.0 g/l Meat extract 10.0 g/l Na-acetate 5.0 g/l Yeast extract 5.0 g/l (NH4)3 citrate 2.0 g/l K2HPO4 2.0 g/l Tween 80 1.0 g/l MgSO4 x 7 H2O 0.2 g/l MnSO4 x H2O 0.05 g/l Distilled waterhttps://mediadive.dsmz.de/medium/11
118879CIP Medium 40yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=40

culture temp

@refgrowthtypetemperaturerange
40059positivegrowth37mesophilic
51994positivegrowth37mesophilic
8502positivegrowth37mesophilic
67770positivegrowth37mesophilic
118879positivegrowth30-45
118879nogrowth10psychrophilic
118879nogrowth15psychrophilic
118879nogrowth22psychrophilic

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
8502anaerobe
8502microaerophile

spore formation

@refspore formationconfidence
69481no100
69480no99.995

murein

  • @ref: 8502
  • murein short key: A11.31
  • type: A4alpha L-Lys-D-Asp

observation

  • @ref: 67770
  • observation: Assay of Alanine and pyridoxal

metabolite utilization

@refmetaboliteutilization activitykind of utilization testedChebi-ID
68371Potassium 5-ketogluconate-builds acid from
68371Potassium 2-ketogluconate-builds acid from
68371gluconate-builds acid from24265
68371L-arabitol-builds acid from18403
68371D-arabitol-builds acid from18333
68371L-fucose-builds acid from18287
68371D-fucose-builds acid from28847
68371D-tagatose-builds acid from16443
68371D-lyxose-builds acid from62318
68371turanose-builds acid from32528
68371gentiobiose-builds acid from28066
68371xylitol-builds acid from17151
68371glycogen-builds acid from28087
68371starch-builds acid from28017
68371raffinose-builds acid from16634
68371melezitose-builds acid from6731
68371inulin-builds acid from15443
68371trehalose-builds acid from27082
68371melibiose-builds acid from28053
68371lactose-builds acid from17716
68371maltose-builds acid from17306
68371cellobiose-builds acid from17057
68371salicin-builds acid from17814
68371arbutin-builds acid from18305
68371amygdalin-builds acid from27613
68371methyl alpha-D-glucopyranoside-builds acid from320061
68371methyl alpha-D-mannoside-builds acid from43943
68371D-sorbitol-builds acid from17924
68371D-mannitol-builds acid from16899
68371myo-inositol-builds acid from17268
68371galactitol-builds acid from16813
68371L-rhamnose-builds acid from62345
68371L-sorbose-builds acid from17266
68371D-galactose-builds acid from12936
68371methyl beta-D-xylopyranoside-builds acid from74863
68371ribitol-builds acid from15963
68371L-xylose-builds acid from65328
68371D-xylose-builds acid from65327
68371D-ribose-builds acid from16988
68371L-arabinose-builds acid from30849
68371D-arabinose-builds acid from17108
68371erythritol-builds acid from17113
68371glycerol-builds acid from17754
118879nitrate-reduction17632
118879nitrite-reduction16301
118879nitrate+respiration17632

antibiotic resistance

  • @ref: 118879
  • metabolite: 0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)
  • is antibiotic: yes
  • is sensitive: no
  • is resistant: yes

metabolite tests

  • @ref: 118879
  • Chebi-ID: 15688
  • metabolite: acetoin
  • voges-proskauer-test: -

enzymes

@refvalueactivityec
68382alpha-fucosidase-3.2.1.51
68382alpha-mannosidase-3.2.1.24
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382beta-glucosidase-3.2.1.21
68382alpha-glucosidase+3.2.1.20
68382beta-glucuronidase-3.2.1.31
68382beta-galactosidase-3.2.1.23
68382alpha-galactosidase-3.2.1.22
68382naphthol-AS-BI-phosphohydrolase+
68382acid phosphatase+3.1.3.2
68382alpha-chymotrypsin-3.4.21.1
68382trypsin-3.4.21.4
68382cystine arylamidase-3.4.11.3
68382valine arylamidase-
68382leucine arylamidase+3.4.11.1
68382lipase (C 14)-
68382esterase lipase (C 8)-
68382esterase (C 4)-
68382alkaline phosphatase-3.1.3.1
118879oxidase-
118879alcohol dehydrogenase-1.1.1.1
118879catalase-1.11.1.6
118879lysine decarboxylase-4.1.1.18
118879ornithine decarboxylase-4.1.1.17

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
118879-----+----++---+----

API 50CHac

@refQGLYERYDARALARARIBDXYLLXYLADOMDXGALGLUFRUMNESBERHADULINOMANSORMDMMDGNAGAMYARBESCSALCELMALLACMELSACTREINUMLZRAFAMDGLYGXLTGENTURLYXTAGDFUCLFUCDARLLARLGNT2KG5KG
8502-----------+++-----------------+------------------
8502-----------+++--------+--------+------------------
8502-----------+++--------+/---------+------------------
8502-----------+++-----------+/------+------------------
8502-----------+++-----------------+------------------
118879----------+/-+/-+/---------+/---------+/-------------------

Isolation, sampling and environmental information

isolation

@refsample type
8502sour grain mash
67770Sour grain mash
118879Sour grain mash

isolation source categories

  • Cat1: #Engineered
  • Cat2: #Food production
  • Cat3: #Food

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
85021Risk group (German classification)
1188791Risk group (French classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Lactobacillus delbrueckii subsp. delbrueckii gene for 16S rRNA, partial sequence, strain: YIT 0080 (= ATCC 9649)AB0082071558ena83684
20218Lactobacillus delbrueckii subsp. delbrueckii ATCC 9649 16S ribosomal RNA gene, partial sequenceAF429502500ena83684
20218Lactobacillus delbrueckii subsp. delbrueckii strain ATCC 9649 16S ribosomal RNA gene, partial sequence; 16S-23S intergenic spacer, complete sequence; and 23S ribosomal RNA gene, partial sequenceAF429589534ena83684
20218Lactobacillus delbrueckii subsp. delbrueckii 16S ribosomal RNA gene, partial sequenceAY0501721507ena83684
20218Lactobacillus delbrueckii subsp. delbrueckii strain BCRC12195 16S ribosomal RNA gene, complete sequenceAY7739491518ena83684
20218Lactobacillus delbrueckii subsp. delbrueckii strain DSM 20074 16S ribosomal RNA gene, partial sequenceEF468101426ena83684
20218Lactobacillus delbrueckii strain DSM 20074 16S ribosomal RNA gene, partial sequenceHM0075901365ena1584
20218Lactobacillus delbrueckii 16S ribosomal RNAM588141512ena1584
20218Lactobacillus delbrueckii subsp. delbrueckii gene for 16S rRNA, partial sequence, strain: JCM 1012AB289084671ena83684
20218Lactobacillus delbrueckii gene for 16S ribosomal RNA, partial sequenceD31686175ena1584
20218Lactobacillus delbrueckii subsp. delbrueckii gene for 16S rRNA, partial sequence, strain: NBRC 3202AB6800271489ena83684
20218L.delbrueckii 16S rRNAX526541488ena1584
67770Lactobacillus delbrueckii subsp. delbrueckii gene for 16S ribosomal RNA, partial sequence, strain: JCM 1012LC0631631525ena83684

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Lactobacillus delbrueckii subsp. delbrueckii DSM 20074 = JCM 1012GCA_001908495chromosomencbi1423823
66792Lactobacillus delbrueckii subsp. delbrueckii DSM 20074 = JCM 10121423823.3wgspatric1423823
66792Lactobacillus delbrueckii subsp. delbrueckii DSM 20074 = JCM 10121423823.4completepatric1423823
66792Lactobacillus delbrueckii subsp. delbrueckii JCM 10121236545.3wgspatric1423823
66792Lactobacillus delbrueckii subsp. delbrueckii strain NBRC 320283684.12wgspatric83684
66792Lactobacillus delbrueckii delbrueckii DSM 200742721755550completeimg1423823
66792Lactobacillus delbrueckii delbrueckii JCM 10122585427925draftimg1423823
66792Lactobacillus delbrueckii delbrueckii DSM 200742657244991draftimg1423823
67770Lactobacillus delbrueckii subsp. delbrueckii NBRC 3202GCA_006740305completencbi83684
67770Lactobacillus delbrueckii subsp. delbrueckii DSM 20074 = JCM 1012GCA_001433875scaffoldncbi1423823
67770Lactobacillus delbrueckii subsp. delbrueckii DSM 20074 = JCM 1012GCA_000615095contigncbi1423823

GC content

@refGC-contentmethod
850250.0
6777050genome sequence analysis
6777050

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingno100no
motileno94.968no
gram-positiveyes95.301no
anaerobicyes53.979no
halophileyes82.177no
spore-formingno96.54no
glucose-utilyes92.659no
thermophileno98.847yes
aerobicno97.364yes
flagellatedno97.94no
glucose-fermentyes87.895no

External links

@ref: 8502

culture collection no.: CCUG 34222, CCM 7191, DSM 20074, ATCC 9649, NCDO 213, NCIB 8130, JCM 1012, BCRC 12195, CECT 286, CGMCC 1.2624, CIP 57.8, IAM 12474, IFO 3202, KCTC 1047, LMG 6412, NBRC 3202, NCIMB 8130, NRIC 1053, NRRL B-763, VKM B-1596, VTT E-98097

straininfo link

  • @ref: 75923
  • straininfo: 9762

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny10508059Use of PCR-based methods for rapid differentiation of Lactobacillus delbrueckii subsp. bulgaricus and L. delbrueckii subsp. lactis.Torriani S, Zapparoli G, Dellaglio FAppl Environ Microbiol10.1128/AEM.65.10.4351-4356.19991999Aminopeptidases/genetics, Lactobacillus/*classification/genetics, *Polymerase Chain Reaction, Random Amplified Polymorphic DNA Technique
Metabolism14467904Phthiocol and menadione as acetate-replacing factors for Lactobacillus delbrueckii.MACIASR FMJ Bacteriol10.1128/jb.82.5.657-661.19611961Acetates/*metabolism, *Iron, Lactobacillus/*metabolism, *Lactobacillus delbrueckii, Naphthoquinones/*metabolism, *Nitrogen, *Oxidation-Reduction, *Oxygen, Vitamin K/*metabolism, *Vitamin K 3
Metabolism16171677Production of D-lactic acid from defatted rice bran by simultaneous saccharification and fermentation.Tanaka T, Hoshina M, Tanabe S, Sakai K, Ohtsubo S, Taniguchi MBioresour Technol10.1016/j.biortech.2005.02.0252005Carbohydrate Metabolism, Carbohydrates, Dietary Fiber/*metabolism, Fermentation/*physiology, Hydrogen-Ion Concentration, Lactic Acid/*biosynthesis, Lactobacillus delbrueckii/metabolism, Oryza/*metabolism, Polysaccharides/metabolism, Time FactorsBiotechnology
Metabolism18343337Bidirectional cell-surface anchoring function of C-terminal repeat region of peptidoglycan hydrolase of Lactococcus lactis IL1403.Tarahomjoo S, Katakura Y, Satoh E, Shioya SJ Biosci Bioeng10.1263/jbb.105.1162008Cell Adhesion/*physiology, Cell Membrane/*metabolism, Lactococcus lactis/*cytology/*physiology, N-Acetylmuramoyl-L-alanine Amidase/genetics/*metabolism, Protein Structure, Tertiary, Recombinant Fusion Proteins/metabolism, Repetitive Sequences, Amino Acid/physiology
Enzymology18623312A reagentless amperometric electrode based on carbon paste, chemically modified with D-lactate dehydrogenase, NAD(+), and mediator containing polymer for D-lactic acid analysis. II. On-line monitoring of fermentation process.Shu HC, Gorton L, Persson B, Mattiasson BBiotechnol Bioeng10.1002/bit.2604603111995Biotechnology
Pathogenicity21718486Antagonistic effect of Lactobacillus strains against gas-producing coliforms isolated from colicky infants.Savino F, Cordisco L, Tarasco V, Locatelli E, Di Gioia D, Oggero R, Matteuzzi DBMC Microbiol10.1186/1471-2180-11-1572011*Antibiosis, Colic/*microbiology, Colony Count, Microbial, Enterobacteriaceae/isolation & purification/*physiology, Feces/microbiology, Female, Gases/metabolism, Gastrointestinal Tract/*microbiology, Humans, Infant, Infant, Newborn, Lactobacillus/*physiology, MaleEnzymology
Biotechnology30264211Bacteriocin production of the probiotic Lactobacillus acidophilus KS400.Gaspar C, Donders GG, Palmeira-de-Oliveira R, Queiroz JA, Tomaz C, Martinez-de-Oliveira J, Palmeira-de-Oliveira AAMB Express10.1186/s13568-018-0679-z2018
Enzymology31794327Batch and fed-batch fermentation of optically pure D (-) lactic acid from Kodo millet (Paspalum scrobiculatum) bran residue hydrolysate: growth and inhibition kinetic modeling.Balakrishnan R, Tadi SRR, Rajaram SK, Mohan N, Sivaprakasam SPrep Biochem Biotechnol10.1080/10826068.2019.16979342019*Fermentation, Hydrolysis, Kinetics, Lactic Acid/chemistry/*metabolism, Lactobacillus delbrueckii/metabolism, Paspalum/*chemistry, Seeds/*chemistryMetabolism
Biotechnology32123412Effect of nitrogen sources and neutralizing agents on D-lactic acid production from Kodo millet bran hydrolysate: comparative study and kinetic analysis.Balakrishnan R, Tadi SRR, Pavan ASS, Sivaprakasam S, Rajaram SJ Food Sci Technol10.1007/s13197-019-04124-72019

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
8502Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 20074)https://www.dsmz.de/collection/catalogue/details/culture/DSM-20074
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
40059Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/9591
51994Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 34222)https://www.ccug.se/strain?id=34222
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68371Automatically annotated from API 50CH acid
68382Automatically annotated from API zym
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch10.21203/rs.3.rs-2527258/v1Predictions based on the model GenomeNet Sporulation v. 1
75923Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID9762.1StrainInfo: A central database for resolving microbial strain identifiers
118879Curators of the CIPCollection of Institut Pasteur (CIP 57.8)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%2057.8