Strain identifier

BacDive ID: 6444

Type strain: Yes

Species: Latilactobacillus curvatus

Strain Designation: 1

Strain history: CIP <- 1987, DSM <- I.G. Abo-Elnaga: strain 1

NCBI tax ID(s): 28038 (species)

« back to strain detail view
For citation purpose refer to the digital object identifier (doi) of the current version.
Archive
version 9.1 (current version):
version 9:
version 8.1:
version 8:
version 7.1:
version 7:
version 6:
version 5:
version 4.1:
version 4:
version 3:
version 2.1:
version 2:
version 1:
version 9.1 (current version)

General

@ref: 8431

BacDive-ID: 6444

DSM-Number: 20019

keywords: genome sequence, 16S sequence, Bacteria, anaerobe, rod-shaped

description: Latilactobacillus curvatus 1 is an anaerobe, rod-shaped bacterium that was isolated from milk.

NCBI tax id

  • NCBI tax id: 28038
  • Matching level: species

strain history

@refhistory
8431<- I. G. Abo-Elnaga, 1
67770T. Mitsuoka S8-19 <-- DSM 20019 <-- I. G. Abo-Elnaga 1.
118822CIP <- 1987, DSM <- I.G. Abo-Elnaga: strain 1

doi: 10.13145/bacdive6444.20240916.9.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Bacilli
  • order: Lactobacillales
  • family: Lactobacillaceae
  • genus: Latilactobacillus
  • species: Latilactobacillus curvatus
  • full scientific name: Latilactobacillus curvatus (Troili-Petersson 1903) Zheng et al. 2020

  • synonyms

    @refsynonym
    20215Bacterium curvatum
    20215Lactobacillus curvatus

@ref: 8431

domain: Bacteria

phylum: Firmicutes

class: Bacilli

order: Lactobacillales

family: Lactobacillaceae

genus: Latilactobacillus

species: Latilactobacillus curvatus

full scientific name: Latilactobacillus curvatus (Troili-Petersson 1903) Zheng et al. 2020

strain designation: 1

type strain: yes

Morphology

cell morphology

@refcell shapemotilitygram stainconfidence
118822rod-shapedno
69480positive91.694

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
8431MRS MEDIUM (DSMZ Medium 11)yeshttps://mediadive.dsmz.de/medium/11Name: MRS MEDIUM (DSMZ Medium 11) Composition: Glucose 20.0 g/l Casein peptone 10.0 g/l Meat extract 10.0 g/l Na-acetate 5.0 g/l Yeast extract 5.0 g/l (NH4)3 citrate 2.0 g/l K2HPO4 2.0 g/l Tween 80 1.0 g/l MgSO4 x 7 H2O 0.2 g/l MnSO4 x H2O 0.05 g/l Distilled water
41331MEDIUM 40- for Lactobacillus and LeuconostocyesDistilled water make up to (1000.000 ml);Man Rogosa Sharp agar (68.000 g)
118822CIP Medium 40yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=40

culture temp

@refgrowthtypetemperature
8431positivegrowth30
41331positivegrowth37
50206positivegrowth30
67770positivegrowth30
118822positivegrowth15-37
118822nogrowth10
118822nogrowth45

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
50206anaerobe
50206microaerophile
118822facultative anaerobe

spore formation

  • @ref: 69481
  • spore formation: no
  • confidence: 100

compound production

  • @ref: 8431
  • compound: lactic acid racemase

murein

  • @ref: 8431
  • murein short key: A11.31
  • type: A4alpha L-Lys-D-Asp

metabolite utilization

@refmetaboliteutilization activitykind of utilization testedChebi-ID
68371Potassium 5-ketogluconate-builds acid from
68371Potassium 2-ketogluconate-builds acid from
68371gluconate-builds acid from24265
68371L-arabitol-builds acid from18403
68371D-arabitol-builds acid from18333
68371L-fucose-builds acid from18287
68371D-fucose-builds acid from28847
68371D-tagatose-builds acid from16443
68371D-lyxose-builds acid from62318
68371gentiobiose-builds acid from28066
68371xylitol-builds acid from17151
68371glycogen-builds acid from28087
68371raffinose-builds acid from16634
68371melezitose-builds acid from6731
68371inulin-builds acid from15443
68371trehalose-builds acid from27082
68371sucrose-builds acid from17992
68371melibiose-builds acid from28053
68371lactose-builds acid from17716
68371maltose+builds acid from17306
68371salicin-builds acid from17814
68371arbutin-builds acid from18305
68371amygdalin-builds acid from27613
68371N-acetylglucosamine+builds acid from59640
68371methyl alpha-D-glucopyranoside-builds acid from320061
68371methyl alpha-D-mannoside-builds acid from43943
68371D-sorbitol-builds acid from17924
68371D-mannitol-builds acid from16899
68371myo-inositol-builds acid from17268
68371galactitol-builds acid from16813
68371L-rhamnose-builds acid from62345
68371L-sorbose-builds acid from17266
68371D-mannose+builds acid from16024
68371D-fructose+builds acid from15824
68371D-glucose+builds acid from17634
68371D-galactose+builds acid from12936
68371methyl beta-D-xylopyranoside-builds acid from74863
68371ribitol-builds acid from15963
68371L-xylose-builds acid from65328
68371D-xylose-builds acid from65327
68371D-ribose+builds acid from16988
68371L-arabinose-builds acid from30849
68371D-arabinose-builds acid from17108
68371erythritol-builds acid from17113
68371glycerol-builds acid from17754
118822nitrate-reduction17632
118822nitrite-reduction16301
118822nitrate+respiration17632

antibiotic resistance

  • @ref: 118822
  • metabolite: 0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)
  • is antibiotic: yes
  • is sensitive: yes
  • is resistant: no

metabolite tests

  • @ref: 118822
  • Chebi-ID: 15688
  • metabolite: acetoin
  • voges-proskauer-test: -

enzymes

@refvalueactivityec
68382alpha-fucosidase-3.2.1.51
68382alpha-mannosidase-3.2.1.24
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382beta-glucosidase-3.2.1.21
68382alpha-glucosidase-3.2.1.20
68382beta-glucuronidase-3.2.1.31
68382beta-galactosidase-3.2.1.23
68382alpha-galactosidase-3.2.1.22
68382naphthol-AS-BI-phosphohydrolase+
68382acid phosphatase+3.1.3.2
68382alpha-chymotrypsin-3.4.21.1
68382trypsin-3.4.21.4
68382cystine arylamidase-3.4.11.3
68382valine arylamidase-
68382leucine arylamidase-3.4.11.1
68382lipase (C 14)-
68382esterase lipase (C 8)-
68382esterase (C 4)-
68382alkaline phosphatase+3.1.3.1
118822oxidase-
118822alcohol dehydrogenase-1.1.1.1
118822catalase-1.11.1.6
118822lysine decarboxylase-4.1.1.18
118822ornithine decarboxylase+4.1.1.17

fatty acid profile

  • fatty acids

    @reffatty acidpercentageECL
    50206C14:03.414
    50206C16:032.116
    50206C18:014.718
    50206C18:1 ω9c18.417.769
    50206C18:2 ω6,9c/C18:0 ANTE31.517.724
  • type of FA analysis: whole cell analysis
  • method/protocol: CCUG

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
118822-+--------++--------

API 50CHac

@refQGLYERYDARALARARIBDXYLLXYLADOMDXGALGLUFRUMNESBERHADULINOMANSORMDMMDGNAGAMYARBESCSALCELMALLACMELSACTREINUMLZRAFAMDGLYGXLTGENTURLYXTAGDFUCLFUCDARLLARLGNT2KG5KG
8431-----+----++++--------+--+/---+---------------------
8431-----+----++++--------+----++---------------------
8431-----+----++++--------+--+-++---------------------
118822----+----++++--------+-----+-------+---+---------

Isolation, sampling and environmental information

isolation

@refsample type
8431milk
50206Milk
67770Milk
118822Food, Milk

isolation source categories

Cat1Cat2Cat3
#Host Body Product#Fluids#Milk
#Engineered#Food production#Dairy product

taxonmaps

  • @ref: 69479
  • File name: preview.99_668.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_45;96_411;97_466;98_537;99_668&stattab=map
  • Last taxonomy: Latilactobacillus
  • 16S sequence: AM113777
  • Sequence Identity:
  • Total samples: 26696
  • soil counts: 1455
  • aquatic counts: 2674
  • animal counts: 21518
  • plant counts: 1049

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
84311Risk group (German classification)
1188221Risk group (French classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Lactobacillus curvatus 16S-23S rRNA intergenic spacer regionAF074858220nuccore28038
20218Lactobacillus curvatus 16S rRNA gene for 16S ribosomal RNAAJ2709511252nuccore28038
20218Lactobacillus curvatus partial 16S rRNA gene, strain type strain:DSM 20019AM1137771559nuccore28038
20218Lactobacillus curvatus 16S/23S ribosomal RNA small intergenic spacer region, complete sequenceU97129222nuccore28038
20218Lactobacillus curvatus 16S/23S ribosomal RNA large intergenic spacer region, tRNA-Ile and tRNA-Ala genes, complete sequenceU97135437nuccore28038
20218Lactobacillus curvatus gene for 16S rRNA, partial sequence, strain: JCM 1091AB289076684nuccore28038
20218Lactobacillus curvatus gene for 16S rRNA, partial sequence, strain: JCM 1096AB289077673nuccore1293592
20218Lactobacillus curvatus gene for 16S ribosomal RNA, partial sequenceD31685178nuccore1293592
20218Lactobacillus curvatus subsp. curvatus partial 16S rRNA gene, strain LMG 9198TAJ6215501401nuccore28038
20218Lactobacillus curvatus gene for 16S rRNA, partial sequence, strain: NBRC 15884AB6260511491nuccore28038
67770Lactobacillus curvatus gene for 16S ribosomal RNA, partial sequence, strain: JCM 1096LC0631671531nuccore1293592

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Latilactobacillus curvatus JCM 1096 = DSM 20019GCA_004101845completencbi1293592
66792Lactobacillus curvatus JCM 1096 = DSM 200191293592.9completepatric1293592
66792Lactobacillus curvatus JCM 1096 = DSM 200191293592.5wgspatric1293592
66792Lactobacillus curvatus JCM 1096 = DSM 200191293592.4wgspatric1293592
66792Lactobacillus curvatus strain NBRC 1588428038.112wgspatric28038
66792Lactobacillus curvatus JCM 1096, DSM 200192728369693draftimg1293592
66792Lactobacillus curvatus DSM 200192663762934draftimg1293592
66792Lactobacillus curvatus DSM 200192877511904completeimg1293592
67770Latilactobacillus curvatus JCM 1096 = DSM 20019GCA_001435495scaffoldncbi1293592
67770Latilactobacillus curvatus JCM 1096 = DSM 20019GCA_001311645contigncbi1293592
67770Latilactobacillus curvatus NBRC 15884GCA_006540285contigncbi28038

GC content

@refGC-contentmethod
843142.9
6777042genome sequence analysis

Genome-based predictions

predictions

@reftraitmodeldescriptionpredictionconfidencetraining_data
69481spore-formingspore-formingAbility to form endo- or exosporesno100no
69480gram-positivegram-positivePositive reaction to Gram-stainingyes91.694no
69480anaerobicanaerobicAbility to grow under anoxygenic conditions (including facultative anaerobes)no87.937no
69480aerobicaerobicAbility to grow under oxygenic conditions (including facultative aerobes)no95.21no
69480spore-formingspore-formingAbility to form endo- or exosporesno88.752no
69480thermophilicthermophileAbility to grow at temperatures above or equal to 45°Cno93.971no
69480flagellatedmotile2+Ability to perform flagellated movementno89.5no

External links

@ref: 8431

culture collection no.: DSM 20019, ATCC 25601, NCIB 9710, CCUG 30669, LMG 9198, JCM 1096, BCRC 12189, CECT 904, CIP 102992, IFO 15884, KCTC 3767, LMG 13553, NBRC 15884, NCFB 2739, NCIMB 9710, NRIC 1052, NRRL B-4562, VTT E-90391, NCDO 2739

straininfo link

  • @ref: 75917
  • straininfo: 92300

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny8863451Lactobacillus curvatus subsp. curvatus subsp. nov. and Lactobacillus curvatus subsp. melibiosus subsp. nov. and Lactobacillus sake subsp. sake subsp. nov. and Lactobacillus sake subsp. carnosus subsp. nov., new subspecies of Lactobacillus curvatus Abo-Elnaga and Kandler 1965 and Lactobacillus sake Katagiri, Kitahara, and Fukami 1934 (Klein et al. 1996, emended descriptions), respectively.Torriani S, Van Reenen GA, Klein G, Reuter G, Dellaglio F, Dicks LMInt J Syst Bacteriol10.1099/00207713-46-4-11581996DNA, Bacterial/analysis, Lactobacillus/*classification/genetics/growth & development, Polymerase Chain Reaction
9925585Hydrolysis of pork muscle sarcoplasmic proteins by lactobacillus curvatus and lactobacillus sake.Fadda S, Sanz Y, Vignolo G, Aristoy M, Oliver G, Toldra FAppl Environ Microbiol10.1128/AEM.65.2.578-584.19991999
Metabolism10634703Hydrolysis of muscle myofibrillar proteins by Lactobacillus curvatus and Lactobacillus sake.Sanz Y, Fadda S, Vignolo G, Aristoy MC, Oliver G, Toldra FInt J Food Microbiol10.1016/s0168-1605(99)00134-81999Aminopeptidases/metabolism, Animals, Chromatography, High Pressure Liquid, Colony Count, Microbial, Electrophoresis, Polyacrylamide Gel, Endopeptidases/metabolism, Fluorometry, *Food Microbiology, Lactobacillus/*enzymology, Meat Products/*microbiology, Muscle Proteins/analysis/*metabolism, Myofibrils/chemistryEnzymology
Biotechnology16313402Effect of Lactobacillus-protective cultures with bacteriocin-like inhibitory substances' producing ability on microbiological, chemical and sensory changes during storage of refrigerated vacuum-packaged sliced beef.Katikou P, Ambrosiadis I, Georgantelis D, Koidis P, Georgakis SAJ Appl Microbiol10.1111/j.1365-2672.2005.02739.x2005Animals, *Antibiosis, *Bacteriocins, Cattle, Food Handling, *Food Microbiology, Food Packaging, Food Preservation/*methods, Humans, *Lactobacillus, *Meat, Refrigeration, VacuumPathogenicity
Phylogeny16347554Isolation of a DNA Probe for Lactobacillus curvatus.Petrick HA, Ambrosio RE, Holzapfel WHAppl Environ Microbiol10.1128/aem.54.2.405-408.19881988
Biotechnology24522411Plantaricin LD1: a bacteriocin produced by food isolate of Lactobacillus plantarum LD1.Gupta A, Tiwari SKAppl Biochem Biotechnol10.1007/s12010-014-0775-82014Anti-Bacterial Agents/*biosynthesis/chemistry/pharmacology, Bacteriocins/*biosynthesis/chemistry/pharmacology, *Food Microbiology, Gram-Negative Bacteria/drug effects, Gram-Positive Bacteria/drug effects, Hydrogen-Ion Concentration, Lactobacillus plantarum/growth & development/isolation & purification/*metabolism, Molecular WeightPathogenicity
Genetics29850855Phylogenomic Analysis of Lactobacillus curvatus Reveals Two Lineages Distinguished by Genes for Fermenting Plant-Derived Carbohydrates.Teran LC, Coeuret G, Raya R, Zagorec M, Champomier-Verges MC, Chaillou SGenome Biol Evol10.1093/gbe/evy1062018Carbohydrates/*genetics, Fermentation/*genetics, Genome, Bacterial/genetics, Genomics/methods, Lactobacillus/*genetics, Meat Products, Multigene Family/genetics, Phylogeny, Polymorphism, Single Nucleotide/geneticsPhylogeny
Transcriptome32240216Nucleotide sequence and analysis of pRC12 and pRC18, two theta-replicating plasmids harbored by Lactobacillus curvatus CRL 705.Teran LC, Cuozzo SA, Aristimuno Ficoseco MC, Fadda S, Chaillou S, Champomier-Verges MC, Zagorec M, Hebert EM, Raya RRPLoS One10.1371/journal.pone.02308572020Amino Acid Sequence/genetics, Bacterial Proteins/genetics, Base Sequence/genetics, DNA Replication/genetics, DNA, Bacterial/genetics, Genetic Vectors/genetics, Lactobacillus/*genetics, Plasmids/*genetics, Replication Origin/genetics, Replicon/genetics, Sequence Analysis, DNA/methods, Transposases/geneticsGenetics
Pathogenicity35305124Mechanism of high D-aspartate production in the lactic acid bacterium Latilactobacillus sp. strain WDN19.Kajitani K, Ishikawa T, Kobayashi T, Asato M, Shibata K, Kouya T, Takahashi SAppl Microbiol Biotechnol10.1007/s00253-022-11870-w2022Asparagine, *Aspartic Acid, D-Aspartic Acid, Humans, Lactic Acid, *Lactobacillales, LactobacillusBiotechnology

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
8431Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 20019)https://www.dsmz.de/collection/catalogue/details/culture/DSM-20019
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
41331Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/14739
50206Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 30669)https://www.ccug.se/strain?id=30669
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68371Automatically annotated from API 50CH acid
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch10.21203/rs.3.rs-2527258/v1Predictions based on the model GenomeNet Sporulation v. 1
75917Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID92300.1StrainInfo: A central database for resolving microbial strain identifiers
118822Curators of the CIPCollection of Institut Pasteur (CIP 102992)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20102992