Strain identifier

BacDive ID: 6416

Type strain: Yes

Species: Ligilactobacillus animalis

Strain Designation: PPI/1535, PP1/1535

Strain history: CIP <- 1988, NCFB <- 1981, R.A.D. Williams: strain PP1/1535

NCBI tax ID(s): 1605 (species)

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General

@ref: 8950

BacDive-ID: 6416

DSM-Number: 20602

keywords: genome sequence, 16S sequence, Bacteria, anaerobe, mesophilic, Gram-positive, rod-shaped

description: Ligilactobacillus animalis PPI/1535 is an anaerobe, mesophilic, Gram-positive bacterium that was isolated from dental plaque of baboon.

NCBI tax id

  • NCBI tax id: 1605
  • Matching level: species

strain history

@refhistory
8950<- NCDO <- V.E. Dent, PPI/1535
67770ATCC 35046 <-- NCDO 2425 <-- R. A. D. Williams 1535.
116392CIP <- 1988, NCFB <- 1981, R.A.D. Williams: strain PP1/1535

doi: 10.13145/bacdive6416.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Bacilli
  • order: Lactobacillales
  • family: Lactobacillaceae
  • genus: Ligilactobacillus
  • species: Ligilactobacillus animalis
  • full scientific name: Ligilactobacillus animalis (Dent and Williams 1983) Zheng et al. 2020

  • synonyms

    • @ref: 20215
    • synonym: Lactobacillus animalis

@ref: 8950

domain: Bacteria

phylum: Firmicutes

class: Bacilli

order: Lactobacillales

family: Lactobacillaceae

genus: Ligilactobacillus

species: Ligilactobacillus animalis

full scientific name: Ligilactobacillus animalis (Dent and Williams 1983) Zheng et al. 2020

strain designation: PPI/1535, PP1/1535

type strain: yes

Morphology

cell morphology

  • @ref: 116392
  • gram stain: positive
  • cell shape: rod-shaped
  • motility: no

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
8950MRS MEDIUM (DSMZ Medium 11)yeshttps://mediadive.dsmz.de/medium/11Name: MRS MEDIUM (DSMZ Medium 11) Composition: Glucose 20.0 g/l Casein peptone 10.0 g/l Meat extract 10.0 g/l Na-acetate 5.0 g/l Yeast extract 5.0 g/l (NH4)3 citrate 2.0 g/l K2HPO4 2.0 g/l Tween 80 1.0 g/l MgSO4 x 7 H2O 0.2 g/l MnSO4 x H2O 0.05 g/l Distilled water
40543MEDIUM 40- for Lactobacillus and LeuconostocyesDistilled water make up to (1000.000 ml);Man Rogosa Sharp agar (68.000 g)
116392CIP Medium 40yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=40
116392CIP Medium 41yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=41

culture temp

@refgrowthtypetemperaturerange
8950positivegrowth37mesophilic
40543positivegrowth37mesophilic
51813positivegrowth37mesophilic
67770positivegrowth37mesophilic
116392positivegrowth22-45
116392nogrowth10psychrophilic
116392nogrowth15psychrophilic

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
8950anaerobe
8950microaerophile
51813anaerobe
51813microaerophile
116392facultative anaerobe

murein

  • @ref: 8950
  • murein short key: A11.31
  • type: A4alpha L-Lys-D-Asp

metabolite utilization

@refmetaboliteutilization activitykind of utilization testedChebi-ID
68371Potassium 5-ketogluconate-builds acid from
68371Potassium 2-ketogluconate-builds acid from
68371gluconate-builds acid from24265
68371L-arabitol-builds acid from18403
68371D-arabitol-builds acid from18333
68371L-fucose-builds acid from18287
68371D-fucose-builds acid from28847
68371D-tagatose-builds acid from16443
68371D-lyxose-builds acid from62318
68371turanose-builds acid from32528
68371gentiobiose+builds acid from28066
68371xylitol-builds acid from17151
68371glycogen-builds acid from28087
68371starch-builds acid from28017
68371raffinose+builds acid from16634
68371melezitose-builds acid from6731
68371inulin-builds acid from15443
68371trehalose-builds acid from27082
68371sucrose+builds acid from17992
68371melibiose+builds acid from28053
68371lactose+builds acid from17716
68371maltose+builds acid from17306
68371cellobiose+builds acid from17057
68371salicin+builds acid from17814
68371esculin+builds acid from4853
68371amygdalin+builds acid from27613
68371N-acetylglucosamine+builds acid from59640
68371methyl alpha-D-glucopyranoside-builds acid from320061
68371methyl alpha-D-mannoside-builds acid from43943
68371D-sorbitol-builds acid from17924
68371D-mannitol-builds acid from16899
68371myo-inositol-builds acid from17268
68371galactitol-builds acid from16813
68371L-rhamnose-builds acid from62345
68371L-sorbose-builds acid from17266
68371D-mannose+builds acid from16024
68371D-fructose+builds acid from15824
68371D-glucose+builds acid from17634
68371D-galactose+builds acid from12936
68371methyl beta-D-xylopyranoside-builds acid from74863
68371ribitol-builds acid from15963
68371L-xylose-builds acid from65328
68371D-xylose-builds acid from65327
68371L-arabinose-builds acid from30849
68371D-arabinose-builds acid from17108
68371erythritol-builds acid from17113
68371glycerol-builds acid from17754
116392nitrate-reduction17632
116392nitrite-reduction16301
116392nitrate+respiration17632

metabolite tests

  • @ref: 116392
  • Chebi-ID: 15688
  • metabolite: acetoin
  • voges-proskauer-test: +

enzymes

@refvalueactivityec
68382alpha-fucosidase-3.2.1.51
68382alpha-mannosidase-3.2.1.24
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382beta-glucosidase-3.2.1.21
68382alpha-glucosidase-3.2.1.20
68382beta-glucuronidase-3.2.1.31
68382beta-galactosidase+3.2.1.23
68382alpha-galactosidase+3.2.1.22
68382naphthol-AS-BI-phosphohydrolase+
68382acid phosphatase+3.1.3.2
68382alpha-chymotrypsin-3.4.21.1
68382trypsin-3.4.21.4
68382cystine arylamidase-3.4.11.3
68382valine arylamidase-
68382leucine arylamidase-3.4.11.1
68382lipase (C 14)-
68382esterase lipase (C 8)-
68382esterase (C 4)-
68382alkaline phosphatase+3.1.3.1
116392oxidase-
116392alcohol dehydrogenase-1.1.1.1
116392catalase-1.11.1.6
116392lysine decarboxylase-4.1.1.18
116392ornithine decarboxylase-4.1.1.17

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
116392-+--------++++------

API 50CHac

@refQGLYERYDARALARARIBDXYLLXYLADOMDXGALGLUFRUMNESBERHADULINOMANSORMDMMDGNAGAMYARBESCSALCELMALLACMELSACTREINUMLZRAFAMDGLYGXLTGENTURLYXTAGDFUCLFUCDARLLARLGNT2KG5KG
8950-----+/-----++++--------++++++++++---+---+----------
116392----+/-----++++--------++-+++++++---+---+----------

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinent
8950dental plaque of baboon
51813Baboon dental plaque
67770Dental plaque of baboon
116392Animal, Baboon, dental plaqueLondonUnited KingdomGBREurope

isolation source categories

Cat1Cat2Cat3
#Host#Mammals#Primates
#Host Body-Site#Oral cavity and airways#Plaque
#Host Body-Site#Oral cavity and airways#Tooth

taxonmaps

  • @ref: 69479
  • File name: preview.99_1521.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_45;96_871;97_1018;98_1201;99_1521&stattab=map
  • Last taxonomy: Ligilactobacillus
  • 16S sequence: LC071814
  • Sequence Identity:
  • Total samples: 39087
  • soil counts: 571
  • aquatic counts: 383
  • animal counts: 37886
  • plant counts: 247

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
89501Risk group (German classification)
1163921Risk group (French classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Lactobacillus animalis 16S ribosomal RNAM588071507ena1605
20218Lactobacillus animalis genes for 16S-23S intergenic spacer region, 23S ribosomal RNA, strain:JCM 5670AB1587652932ena930942
20218Lactobacillus animalis gene for 16S rRNA, partial sequence, strain: JCM 5670AB289042688ena930942
20218Lactobacillus animalis gene for 16S rRNA, partial sequence, strain: NBRC 15882AB3263501489ena930942
20218L.animalis 16S rRNA geneX611331406ena930942
67770Lactobacillus animalis gene for 16S ribosomal RNA, partial sequence, strain: JCM 5670LC0718141458ena930942

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Lactobacillus animalis KCTC 3501930942.3wgspatric930942
66792Lactobacillus animalis KCTC 3501 = DSM 20602930942.11wgspatric930942
66792Ligilactobacillus animalis 1535, KCTC 3501651324057draftimg930942
66792Ligilactobacillus animalis DSM 206022657245445draftimg930942
67770Ligilactobacillus animalis KCTC 3501 = DSM 20602GCA_000183825scaffoldncbi930942
67770Ligilactobacillus animalis KCTC 3501 = DSM 20602GCA_001434535scaffoldncbi930942

GC content

@refGC-contentmethod
895042.4
6777041.1genome sequence analysis

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
motileno96.042no
flagellatedno98.352no
gram-positiveyes94.775no
anaerobicno91.252no
halophileyes89.522no
spore-formingno93.344no
glucose-utilyes92.575no
aerobicno98.133no
thermophileno98.189yes
glucose-fermentyes90.205no

External links

@ref: 8950

culture collection no.: DSM 20602, ATCC 35046, NCDO 2425, CCUG 33906, JCM 5670, BCRC 14617, CECT 4060, CGMCC 1.2623, CIP 103152, IAM 13547, IFO 15882, KCTC 3501, LMG 9843, NBRC 15882, NCFB 2425, NCIMB 13278, NRRL B-14176, VTT E-991268

straininfo link

  • @ref: 75891
  • straininfo: 13695

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny16825652Lactobacillus apodemi sp. nov., a tannase-producing species isolated from wild mouse faeces.Osawa R, Fujisawa T, Pukall RInt J Syst Evol Microbiol10.1099/ijs.0.64147-02006Animals, Bacterial Infections/*microbiology/*veterinary, Base Composition, Carbohydrate Metabolism, Carboxylic Ester Hydrolases/*biosynthesis, Cell Wall/chemistry, DNA Fingerprinting, DNA, Bacterial/chemistry/genetics, DNA, Ribosomal/chemistry/genetics, Feces/*microbiology, Genes, rRNA, Lactobacillus/*classification/cytology/*isolation & purification/physiology, Molecular Sequence Data, Murinae/*microbiology, Nucleic Acid Hybridization, Peptidoglycan/chemistry, Phylogeny, RNA, Bacterial/genetics, RNA, Ribosomal, 16S/genetics, Ribotyping, Sequence Analysis, DNA, Sequence Homology, Nucleic Acid, Spores, BacterialGenetics
Genetics21183665Genome sequence of Lactobacillus animalis KCTC 3501.Nam SH, Choi SH, Kang A, Kim DW, Kim RN, Kim A, Kim DS, Park HSJ Bacteriol10.1128/JB.01505-102010Food Microbiology, *Genome, Bacterial, Lactobacillus/classification/*genetics, Molecular Sequence DataBiotechnology

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
8950Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 20602)https://www.dsmz.de/collection/catalogue/details/culture/DSM-20602
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
40543Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/14918
51813Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 33906)https://www.ccug.se/strain?id=33906
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
66794Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg10.1093/nar/gkaa1025BRENDA, the ELIXIR core data resource in 2021: new developments and updatesNucleic Acids Res. 49: D498-D508 202033211880
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68371Automatically annotated from API 50CH acid
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
75891Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID13695.1StrainInfo: A central database for resolving microbial strain identifiers
116392Curators of the CIPCollection of Institut Pasteur (CIP 103152)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20103152