Strain identifier

BacDive ID: 6402

Type strain: Yes

Species: Lactobacillus acetotolerans

Strain history: CIP <- 1988, JCM <- Nakano Biochem. Res. Inst., Japan: strain NBI 3014

NCBI tax ID(s): 1423714 (strain), 1600 (species)

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General

@ref: 9058

BacDive-ID: 6402

DSM-Number: 20749

keywords: genome sequence, 16S sequence, Bacteria, anaerobe, mesophilic, Gram-positive, rod-shaped, lactate production

description: Lactobacillus acetotolerans DSM 20749 is an anaerobe, mesophilic, Gram-positive bacterium that produces lactate and was isolated from fermented vinegar broth.

NCBI tax id

NCBI tax idMatching level
1600species
1423714strain

strain history

@refhistory
9058<- JCM <- E. Entani, NBI 3014
67770E. Entani NBI 3014.
115991CIP <- 1988, JCM <- Nakano Biochem. Res. Inst., Japan: strain NBI 3014

doi: 10.13145/bacdive6402.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Bacilli
  • order: Lactobacillales
  • family: Lactobacillaceae
  • genus: Lactobacillus
  • species: Lactobacillus acetotolerans
  • full scientific name: Lactobacillus acetotolerans Entani et al. 1986

@ref: 9058

domain: Bacteria

phylum: Firmicutes

class: Bacilli

order: Lactobacillales

family: Lactobacillaceae

genus: Lactobacillus

species: Lactobacillus acetotolerans

full scientific name: Lactobacillus acetotolerans Entani et al. 1986

type strain: yes

Morphology

cell morphology

  • @ref: 115991
  • gram stain: positive
  • cell shape: rod-shaped
  • motility: no

colony morphology

  • @ref: 9058
  • incubation period: 3-7 days

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
9058DIFCO RAKA-RAY NO.3 MEDIUM (DSMZ Medium 1047)yeshttps://mediadive.dsmz.de/medium/1047Name: DIFCO RAKA-RAY NO.3 MEDIUM (DSMZ Medium 1047) Composition: Tryptone 20.0 g/l Agar 16.0 g/l Maltose 10.0 g/l Yeast extract 5.0 g/l Glucose 5.0 g/l Fructose 5.0 g/l Potassium Glutamate 2.5 g/l Potassium Aspartate 2.5 g/l Dipotassium Phosphate 2.0 g/l Betaine Hydrochloride 2.0 g/l Diammonium Citrate 2.0 g/l Liver Concentrate 1.0 g/l Magnesium sulfate 0.98 g/l N-Acetylglucosamine 0.5 g/l Manganese Sulfate 0.42 g/l Distilled water
9058PEDIOCOCCUS DAMNOSUS MEDIUM (DSMZ Medium 231)yeshttps://mediadive.dsmz.de/medium/231Name: PEDIOCOCCUS DAMNOSUS MEDIUM (DSMZ Medium 231) Composition: Glucose 20.0 g/l Casein peptone 10.0 g/l Meat extract 10.0 g/l Yeast extract 5.0 g/l Na-acetate 5.0 g/l K2HPO4 2.0 g/l (NH4)3 citrate 2.0 g/l Tween 80 1.0 g/l Cysteine hydrochloride 0.5 g/l MgSO4 x 7 H2O 0.2 g/l MnSO4 x H2O 0.05 g/l Distilled water
40555MEDIUM 40- for Lactobacillus and LeuconostocyesDistilled water make up to (1000.000 ml);Man Rogosa Sharp agar (68.000 g)
115991CIP Medium 40yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=40

culture temp

@refgrowthtypetemperaturerange
9058positivegrowth30mesophilic
40555positivegrowth37mesophilic
50912positivegrowth30mesophilic
67770positivegrowth30mesophilic
115991positivegrowth22-45
115991nogrowth10psychrophilic
115991nogrowth15psychrophilic

culture pH

  • @ref: 50912
  • ability: positive
  • type: growth
  • pH: 5.3

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
50912anaerobe
115991facultative anaerobe

compound production

  • @ref: 9058
  • compound: DL lactic acid

murein

  • @ref: 9058
  • murein short key: A11.31
  • type: A4alpha L-Lys-D-Asp

metabolite utilization

@refmetaboliteutilization activitykind of utilization testedChebi-ID
68371Potassium 5-ketogluconate-builds acid from
68371Potassium 2-ketogluconate-builds acid from
68371gluconate-builds acid from24265
68371L-arabitol-builds acid from18403
68371D-arabitol-builds acid from18333
68371L-fucose-builds acid from18287
68371D-fucose-builds acid from28847
68371D-tagatose-builds acid from16443
68371D-lyxose-builds acid from62318
68371turanose-builds acid from32528
68371gentiobiose-builds acid from28066
68371xylitol-builds acid from17151
68371glycogen-builds acid from28087
68371starch-builds acid from28017
68371raffinose-builds acid from16634
68371melezitose-builds acid from6731
68371inulin-builds acid from15443
68371trehalose+builds acid from27082
68371sucrose-builds acid from17992
68371melibiose-builds acid from28053
68371lactose-builds acid from17716
68371cellobiose-builds acid from17057
68371esculin-builds acid from4853
68371arbutin-builds acid from18305
68371amygdalin-builds acid from27613
68371N-acetylglucosamine+builds acid from59640
68371methyl alpha-D-glucopyranoside-builds acid from320061
68371methyl alpha-D-mannoside-builds acid from43943
68371D-sorbitol-builds acid from17924
68371D-mannitol+builds acid from16899
68371myo-inositol-builds acid from17268
68371galactitol-builds acid from16813
68371L-rhamnose-builds acid from62345
68371L-sorbose-builds acid from17266
68371D-mannose+builds acid from16024
68371D-fructose+builds acid from15824
68371D-glucose+builds acid from17634
68371D-galactose-builds acid from12936
68371methyl beta-D-xylopyranoside-builds acid from74863
68371ribitol-builds acid from15963
68371L-xylose-builds acid from65328
68371D-xylose-builds acid from65327
68371D-ribose-builds acid from16988
68371L-arabinose-builds acid from30849
68371D-arabinose-builds acid from17108
68371erythritol-builds acid from17113
68371glycerol-builds acid from17754
115991nitrate-reduction17632
115991nitrite-reduction16301
115991nitrate+respiration17632

metabolite production

  • @ref: 115991
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

metabolite tests

  • @ref: 115991
  • Chebi-ID: 15688
  • metabolite: acetoin
  • voges-proskauer-test: -

enzymes

@refvalueactivityec
68382alpha-fucosidase-3.2.1.51
68382alpha-mannosidase+3.2.1.24
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382beta-glucosidase+3.2.1.21
68382alpha-glucosidase+3.2.1.20
68382beta-glucuronidase-3.2.1.31
68382beta-galactosidase-3.2.1.23
68382alpha-galactosidase-3.2.1.22
68382naphthol-AS-BI-phosphohydrolase+
68382acid phosphatase+3.1.3.2
68382alpha-chymotrypsin-3.4.21.1
68382trypsin-3.4.21.4
68382cystine arylamidase+3.4.11.3
68382valine arylamidase-
68382leucine arylamidase+3.4.11.1
68382lipase (C 14)-
68382esterase lipase (C 8)-
68382esterase (C 4)+
68382alkaline phosphatase+3.1.3.1
115991oxidase-
115991alcohol dehydrogenase-1.1.1.1
115991catalase-1.11.1.6
115991lysine decarboxylase-4.1.1.18
115991ornithine decarboxylase-4.1.1.17
115991urease-3.5.1.5

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
115991-++--+-+--++---++-+-

API 50CHac

@refQGLYERYDARALARARIBDXYLLXYLADOMDXGALGLUFRUMNESBERHADULINOMANSORMDMMDGNAGAMYARBESCSALCELMALLACMELSACTREINUMLZRAFAMDGLYGXLTGENTURLYXTAGDFUCLFUCDARLLARLGNT2KG5KG
9058-----------+++----+---+---+-+---+-----------------
115991----------+++----+---+-----+/----+-----------------

Isolation, sampling and environmental information

isolation

@refsample typecountryorigin.countrycontinent
9058fermented vinegar broth
50912Turbid broth of rice vinegar
67770Turbid broth of rice vinegar
115991Food, Turbid broth of rice vinegarJapanJPNAsia

isolation source categories

  • Cat1: #Engineered
  • Cat2: #Food production

taxonmaps

  • @ref: 69479
  • File name: preview.99_7067.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_46;96_3312;97_4062;98_5193;99_7067&stattab=map
  • Last taxonomy: Lactobacillus acetotolerans subclade
  • 16S sequence: LC071813
  • Sequence Identity:
  • Total samples: 9628
  • soil counts: 1475
  • aquatic counts: 1136
  • animal counts: 6619
  • plant counts: 398

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
90581Risk group (German classification)
1159911Risk group (French classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Lactobacillus acetotolerans ATCC 43578 16S ribosomal RNA gene, partial sequenceAF429492506ena1600
20218Lactobacillus acetotolerans strain ATCC 43578 16S ribosomal RNA gene, partial sequence; 16S-23S intergenic spacer, complete sequence; and 23S ribosomal RNA gene, partial sequenceAF429579527ena1600
20218Lactobacillus acetotolerans partial 16S rRNA gene, type strain JCM 3825TFR6830991503ena1423714
20218Lactobacillus acetotolerans 16S ribosomal RNA sequenceM588011518ena1600
20218Lactobacillus acetotolerans gene for 16S rRNA, partial sequence, strain: JCM 3825AB289007674ena1423714
20218Lactobacillus acetotolerans gene for 16S ribosomal RNA, partial sequence, strain: JCM 3825AB3038411485ena1423714
67770Lactobacillus acetotolerans gene for 16S ribosomal RNA, partial sequence, strain: JCM 3825LC0718131461ena1423714

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Lactobacillus acetotolerans DSM 20749 = JCM 3825GCA_014648715contigncbi1423714
66792Lactobacillus acetotolerans DSM 20749 = JCM 3825GCA_001311725contigncbi1423714
66792Lactobacillus acetotolerans DSM 20749 = JCM 38251423714.4wgspatric1423714
66792Lactobacillus acetotolerans DSM 20749 = JCM 38251423714.7wgspatric1423714
66792Lactobacillus acetotolerans DSM 20749 = JCM 38251423714.3wgspatric1423714
66792Lactobacillus acetotolerans DSM 207492700989123draftimg1423714
66792Lactobacillus acetotolerans JCM 38252681813023draftimg1423714
67770Lactobacillus acetotolerans DSM 20749 = JCM 3825GCA_001436775scaffoldncbi1423714

GC content

@refGC-contentmethod
905835.3
6777035.3thermal denaturation, midpoint method (Tm)
6777036.3genome sequence analysis

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
motileno96.013no
gram-positiveyes97.025no
anaerobicyes80.521no
halophileyes79.001no
spore-formingno97.668no
glucose-utilyes93.773no
thermophileno96.973yes
aerobicno98.025yes
flagellatedno98.93no
glucose-fermentyes89.781no

External links

@ref: 9058

culture collection no.: DSM 20749, ATCC 43578, JCM 3825, CCUG 32229, LMG 10751, CIP 103180, NCIMB 12799, NBI 3014, CECT 4019, NCFB 2798

straininfo link

  • @ref: 75875
  • straininfo: 3186

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
9058Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 20749)https://www.dsmz.de/collection/catalogue/details/culture/DSM-20749
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
40555Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/14949
50912Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 32229)https://www.ccug.se/strain?id=32229
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
66794Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg10.1093/nar/gkaa1025BRENDA, the ELIXIR core data resource in 2021: new developments and updatesNucleic Acids Res. 49: D498-D508 202033211880
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68371Automatically annotated from API 50CH acid
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
75875Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID3186.1StrainInfo: A central database for resolving microbial strain identifiers
115991Curators of the CIPCollection of Institut Pasteur (CIP 103180)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20103180