Name: Lactobacillus acetotolerans DSM 20749
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 6402

Type strain:

Species: Lactobacillus acetotolerans

Culture col. no.: DSM 20749, ATCC 43578, JCM 3825, CCUG 32229, LMG 10751, CIP 103180, NCIMB 12799, NBI 3014, CECT 4019, NCFB 2798

Strain history: E. Entani NBI 3014.

NCBI tax ID(s): 1423714 (strain), 1600 (species)

Other strains from Lactobacillus acetotolerans

L. acetotolerans S-34, DSM 20351, ATCC 27745, IFO ...
L. acetotolerans JCM 33214
L. acetotolerans JCM 9904

Section

Lactobacillus acetotolerans DSM 20749 is an anaerobe, mesophilic bacterium that produces lactate and was isolated from fermented vinegar broth.

lactate production
anaerobe
mesophilic
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	07-12-2022 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Bacillota*
	Class	*Bacilli*
	Order	*Lactobacillales*
	Family	*Lactobacillaceae*
	Genus	*Lactobacillus*
	Species	**Lactobacillus acetotolerans**
	Full Scientific Name (PNU)	Lactobacillus acetotolerans Entani et al. 1986

Information on morphological and physiological properties Morphology

[Ref.: #9058]

Incubation period

3-7 days

Information on culture and growth conditions Culture and growth conditions

[Ref.: #9058]

Culture medium

DIFCO RAKA-RAY NO.3 MEDIUM (DSMZ Medium 1047)

[Ref.: #9058]

Culture medium growth

[Ref.: #9058]

Culture medium link

Medium recipe provided by DSMZ

[Ref.: #9058]

Culture medium composition

expand / minimize

[Ref.: #9058]

Culture medium

PEDIOCOCCUS DAMNOSUS MEDIUM (DSMZ Medium 231)

[Ref.: #9058]

Culture medium growth

[Ref.: #9058]

Culture medium link

Medium recipe provided by DSMZ

[Ref.: #9058]

Culture medium composition

expand / minimize

[Ref.: #40555]

Culture medium

MEDIUM 40- for Lactobacillus and Leuconostoc

[Ref.: #40555]

Culture medium growth

[Ref.: #40555]

Culture medium composition

expand / minimize

		Temperature
[Ref.: #9058]	growth	30 °C
[Ref.: #40555]	growth	37 °C
[Ref.: #50912]	growth	30 °C
[Ref.: #67770]	growth	30 °C

[Ref.: #9058]	Temperature range	mesophilic
[Ref.: #40555]	Temperature range	mesophilic
[Ref.: #50912]	Temperature range	mesophilic
[Ref.: #67770]	Temperature range	mesophilic

	pH	Kind of pH		pH
[Ref.: #50912]			growth	5.3

Information on physiology and metabolism Physiology and metabolism

[Ref.: #50912]

Oxygen tolerance

anaerobe

[Ref.: #9058]

Name of produced compound

DL lactic acid

[Ref.: #9058]	Murein short key	A11.31
[Ref.: #9058]	Murein types	A4alpha L-Lys-D-Asp

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #68371]	amygdalin ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	arbutin ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	cellobiose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-arabinose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-arabitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-fructose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-fucose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-galactose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-glucose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-lyxose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-mannitol ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-mannose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-ribose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-sorbitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-tagatose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-xylose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	erythritol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	esculin ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	galactitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	gentiobiose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	gluconate ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	glycerol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	glycogen ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	inulin ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-arabinose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-arabitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-fucose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-rhamnose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-sorbose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-xylose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	lactose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	maltose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	melezitose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	melibiose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	methyl alpha-D-glucopyranoside ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	methyl alpha-D-mannoside ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	methyl beta-D-xylopyranoside ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	myo-inositol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	N-acetylglucosamine ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	Potassium 2-ketogluconate ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	Potassium 5-ketogluconate ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	raffinose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	ribitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	salicin ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	starch ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	sucrose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	trehalose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	turanose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	xylitol ChEBI	-	builds acid from	* from API 50CH acid
	Only first 10 entries are displayed. Click here to see all.

API® 50CH acid:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	ControlQ	Acid from glycerolGLY	Acid from erythritolERY	Acid from D-arabinoseDARA	Acid from L-arabinoseLARA	Acid from D-riboseRIB	Acid from D-xyloseDXYL	Acid from L-xyloseLXYL	Acid from D-adonitolADO	Acid from methyl beta-D-xylopyranosideMDX	Acid from D-galactoseGAL	Acid from D-glucoseGLU	Acid from D-fructoseFRU	Acid from D-mannoseMNE	Acid from L-sorboseSBE	Acid from L-rhamnoseRHA	Acid from dulcitolDUL	Acid from inositolINO	Acid from D-mannitolMAN	Acid from D-sorbitolSOR	Acid from methyl alpha-D-mannopyranosideMDM	Acid from methyl alpha-D-glucopyranosideMDG	Acid from N-acetyl-glucosamineNAG	Acid from amygdalinAMY	Acid from arbutinARB	Acid from esculinESC	Acid from salicinSAL	Acid from cellobioseCEL	Acid from maltoseMAL	Acid from lactoseLAC	Acid from melibioseMEL	Acid from sucroseSAC	Acid from trehaloseTRE	Acid from inulinINU	Acid from melezitoseMLZ	Acid from raffinoseRAF	Acid from starchAMD	Acid from glycogenGLYG	Acid from xylitolXLT	Acid from gentiobioseGEN	Acid from turanoseTUR	Acid from D-lyxoseLYX	Acid from D-tagatoseTAG	Acid from D-fucoseDFUC	Acid from L-fucoseLFUC	Acid from D-arabitolDARL	Acid from L-arabitolLARL	Acid from gluconateGNT	Acid from 2-Ketogluconate2KG	Acid from 5-Ketogluconate5KG
[Ref.: #9058]	8384	-	-	-	-	-	-	-	-	-	-	-	+	+	+	-	-	-	-	+	-	-	-	+	-	-	-	+	-	+	-	-	-	+	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-

	Pathway	Enzyme coverage	Annotated reactions
	Pathway annotation	Computationally determined by
[Ref.: #66794]	ppGpp biosynthesis	100	4 of 4
[Ref.: #66794]	CDP-diacylglycerol biosynthesis	100	2 of 2
[Ref.: #66794]	palmitate biosynthesis	100	22 of 22
[Ref.: #66794]	acetate fermentation	100	4 of 4
[Ref.: #66794]	methylglyoxal degradation	100	5 of 5
[Ref.: #66794]	cis-vaccenate biosynthesis	100	2 of 2
[Ref.: #66794]	teichoic acid biosynthesis	100	1 of 1
[Ref.: #66794]	anapleurotic synthesis of oxalacetate	100	1 of 1
[Ref.: #66794]	coenzyme A metabolism	100	4 of 4
[Ref.: #66794]	UDP-GlcNAc biosynthesis	100	3 of 3
[Ref.: #66794]	pentose phosphate pathway	81.82	9 of 11
[Ref.: #66794]	aspartate and asparagine metabolism	77.78	7 of 9
[Ref.: #66794]	vitamin B1 metabolism	76.92	10 of 13
[Ref.: #66794]	butanoate fermentation	75	3 of 4
[Ref.: #66794]	ketogluconate metabolism	75	6 of 8
[Ref.: #66794]	propanol degradation	71.43	5 of 7
[Ref.: #66794]	cardiolipin biosynthesis	71.43	5 of 7
[Ref.: #66794]	starch degradation	70	7 of 10
[Ref.: #66794]	L-lactaldehyde degradation	66.67	2 of 3
[Ref.: #66794]	formaldehyde oxidation	66.67	2 of 3
[Ref.: #66794]	acetoin degradation	66.67	2 of 3
[Ref.: #66794]	CO2 fixation in Crenarchaeota	66.67	6 of 9
[Ref.: #66794]	peptidoglycan biosynthesis	66.67	10 of 15
[Ref.: #66794]	C4 and CAM-carbon fixation	62.5	5 of 8
[Ref.: #66794]	degradation of sugar alcohols	62.5	10 of 16
[Ref.: #66794]	gluconeogenesis	62.5	5 of 8
[Ref.: #66794]	phenylalanine metabolism	61.54	8 of 13
[Ref.: #66794]	metabolism of amino sugars and derivatives	60	3 of 5
[Ref.: #66794]	pyrimidine metabolism	60	27 of 45
[Ref.: #66794]	glycogen metabolism	60	3 of 5
[Ref.: #66794]	cellulose degradation	60	3 of 5
[Ref.: #66794]	purine metabolism	59.57	56 of 94
[Ref.: #66794]	glycolysis	58.82	10 of 17
[Ref.: #66794]	photosynthesis	57.14	8 of 14
[Ref.: #66794]	citric acid cycle	57.14	8 of 14
[Ref.: #66794]	d-mannose degradation	55.56	5 of 9
[Ref.: #66794]	serine metabolism	55.56	5 of 9
[Ref.: #66794]	oxidative phosphorylation	53.85	49 of 91
[Ref.: #66794]	dolichol and dolichyl phosphate biosynthesis	50	1 of 2
[Ref.: #66794]	glycogen biosynthesis	50	2 of 4
[Ref.: #66794]	ethanol fermentation	50	1 of 2
[Ref.: #66794]	6-hydroxymethyl-dihydropterin diphosphate biosynthesis	50	4 of 8
[Ref.: #66794]	glycolate and glyoxylate degradation	50	3 of 6
[Ref.: #66794]	isoleucine metabolism	50	4 of 8
[Ref.: #66794]	ribulose monophosphate pathway	50	1 of 2
[Ref.: #66794]	sulfopterin metabolism	50	2 of 4
[Ref.: #66794]	non-pathway related	47.37	18 of 38
[Ref.: #66794]	glutamate and glutamine metabolism	46.43	13 of 28
[Ref.: #66794]	NAD metabolism	44.44	8 of 18
[Ref.: #66794]	mevalonate metabolism	42.86	3 of 7
[Ref.: #66794]	methionine metabolism	42.31	11 of 26
[Ref.: #66794]	propionate fermentation	40	4 of 10
[Ref.: #66794]	ethylmalonyl-CoA pathway	40	2 of 5
[Ref.: #66794]	arachidonate biosynthesis	40	2 of 5
[Ref.: #66794]	Entner Doudoroff pathway	40	4 of 10
[Ref.: #66794]	lipid metabolism	38.71	12 of 31
[Ref.: #66794]	dTDPLrhamnose biosynthesis	37.5	3 of 8
[Ref.: #66794]	metabolism of disaccharids	36.36	4 of 11
[Ref.: #66794]	d-xylose degradation	36.36	4 of 11
[Ref.: #66794]	tyrosine metabolism	35.71	5 of 14
[Ref.: #66794]	degradation of pentoses	35.71	10 of 28
[Ref.: #66794]	alanine metabolism	34.48	10 of 29
[Ref.: #66794]	cyanate degradation	33.33	1 of 3
[Ref.: #66794]	flavin biosynthesis	33.33	5 of 15
[Ref.: #66794]	octane oxidation	33.33	1 of 3
[Ref.: #66794]	valine metabolism	33.33	3 of 9
[Ref.: #66794]	IAA biosynthesis	33.33	1 of 3
[Ref.: #66794]	lipid A biosynthesis	33.33	3 of 9
[Ref.: #66794]	selenocysteine biosynthesis	33.33	2 of 6
[Ref.: #66794]	urea cycle	30.77	4 of 13
[Ref.: #66794]	reductive acetyl coenzyme A pathway	28.57	2 of 7
[Ref.: #66794]	glutathione metabolism	28.57	4 of 14
[Ref.: #66794]	degradation of hexoses	27.78	5 of 18
[Ref.: #66794]	cysteine metabolism	27.78	5 of 18
[Ref.: #66794]	proline metabolism	27.27	3 of 11
[Ref.: #66794]	tryptophan metabolism	26.32	10 of 38
[Ref.: #66794]	cyclohexanol degradation	25	1 of 4
[Ref.: #66794]	lactate fermentation	25	1 of 4
[Ref.: #66794]	CMP-KDO biosynthesis	25	1 of 4
[Ref.: #66794]	degradation of aromatic, nitrogen containing compounds	25	3 of 12
[Ref.: #66794]	leucine metabolism	23.08	3 of 13
[Ref.: #66794]	isoprenoid biosynthesis	23.08	6 of 26
[Ref.: #66794]	chorismate metabolism	22.22	2 of 9
[Ref.: #66794]	nitrate assimilation	22.22	2 of 9
[Ref.: #66794]	4-hydroxymandelate degradation	22.22	2 of 9
[Ref.: #66794]	lysine metabolism	21.43	9 of 42
[Ref.: #66794]	tetrahydrofolate metabolism	21.43	3 of 14
[Ref.: #66794]	arginine metabolism	20.83	5 of 24
[Ref.: #66794]	histidine metabolism	20.69	6 of 29
		Only first 10 entries are displayed. Click here to see all.

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #9058]	Sample type/isolated from	fermented vinegar broth

[Ref.: #50912]	Sample type/isolated from	Turbid broth of rice vinegar

[Ref.: #67770]	Sample type/isolated from	Turbid broth of rice vinegar
* marker position based on {}

Isolation sources categories

#Engineered

#Food production

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence LC071813 (>99% sequence identity) for Lactobacillus acetotolerans subclade from Microbeatlas

Aquatic Samples	1136
Soil Samples	1475
Animal Samples	6619
Plant Samples	398
Total Samples	9628

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #9058]

Biosafety level

Risk group (German classification)
According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

Only first 5 entries are displayed. Click here to see all.

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #67770]	Lactobacillus acetotolerans gene for 16S ribosomal RNA, partial sequence, strain: JCM 3825	LC071813	1461	ENA	1423714 tax ID
[Ref.: #20218]	Lactobacillus acetotolerans ATCC 43578 16S ribosomal RNA gene, partial sequence	AF429492	506	ENA	1600 tax ID	*
[Ref.: #20218]	Lactobacillus acetotolerans strain ATCC 43578 16S ribosomal RNA gene, partial sequence; 16S-23S intergenic spacer, complete sequence; and 23S ribosomal RNA gene, partial sequence	AF429579	527	ENA	1600 tax ID	*
[Ref.: #20218]	Lactobacillus acetotolerans partial 16S rRNA gene, type strain JCM 3825T	FR683099	1503	ENA	1423714 tax ID	*
[Ref.: #20218]	Lactobacillus acetotolerans 16S ribosomal RNA sequence	M58801	1518	ENA	1600 tax ID	*
[Ref.: #20218]	Lactobacillus acetotolerans gene for 16S rRNA, partial sequence, strain: JCM 3825	AB289007	674	ENA	1423714 tax ID	*
[Ref.: #20218]	Lactobacillus acetotolerans gene for 16S ribosomal RNA, partial sequence, strain: JCM 3825	AB303841	1485	ENA	1423714 tax ID	*

Genome sequence information:

Only first 5 entries are displayed. Click here to see all.

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #67770]	Lactobacillus acetotolerans DSM 20749 = JCM 3825 DSM 20749	GCA_001436775	scaffold	GenBank	1423714 tax ID
[Ref.: #66792]	Lactobacillus acetotolerans DSM 20749 = JCM 3825 JCM 3825	GCA_001311725	contig	GenBank	1423714 tax ID	*
[Ref.: #66792]	Lactobacillus acetotolerans DSM 20749 = JCM 3825	1423714.4	wgs	PATRIC	1423714 tax ID	*
[Ref.: #66792]	Lactobacillus acetotolerans DSM 20749 = JCM 3825	1423714.7	wgs	PATRIC	1423714 tax ID	*
[Ref.: #66792]	Lactobacillus acetotolerans DSM 20749 = JCM 3825	1423714.3	wgs	PATRIC	1423714 tax ID	*
[Ref.: #66792]	Lactobacillus acetotolerans DSM 20749	2700989123	draft	JGI IMG/M	1423714 tax ID	*

[Ref.: #9058]	GC-content	35.3 mol%
[Ref.: #67770]	GC-content	35.3 mol%	thermal denaturation, midpoint method (Tm)
[Ref.: #67770]	GC-content	36.3 mol%	genome sequence analysis

Availability in culture collections External links

[Ref.: #9058]

Culture collection no.

DSM 20749, ATCC 43578, JCM 3825, CCUG 32229, LMG 10751, CIP 103180, NCIMB 12799, NBI 3014, CECT 4019, NCFB 2798

[Ref.: #20218]

Associated Passport(s) in StrainInfo

13001, 12999, 13003

References

#9058

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 20749

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#20218

Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )

#40555 ; Curators of the CIP;
#50912 Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 32229

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#66794

Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg: BRENDA, the ELIXIR core data resource in 2021: new developments and updates. Nucleic Acids Res. 49: D498 - D508 2020 ( DOI 10.1093/nar/gkaa1025 , PubMed 33211880 )

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;

#68371

Automatically annotated from API 50CH acid .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

* These data were automatically processed and therefore are not curated

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