Strain identifier

BacDive ID: 6339

Type strain: Yes

Species: Anaerostipes caccae

Strain Designation: L1-92

Strain history: CIP <- 2005, J.P. Carlier, Inst. Pasteur, Paris, France: strain AIP 10223 <- DSM <- S. Duncan: strain L1-92

NCBI tax ID(s): 411490 (strain), 105841 (species)

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General

@ref: 5469

BacDive-ID: 6339

DSM-Number: 14662

keywords: genome sequence, 16S sequence, Bacteria, anaerobe, mesophilic, Gram-positive

description: Anaerostipes caccae L1-92 is an anaerobe, mesophilic, Gram-positive bacterium that was isolated from human faeces.

NCBI tax id

NCBI tax idMatching level
411490strain
105841species

strain history

@refhistory
5469<- S. Duncan; L1-92
67770DSM 14662 <-- S. Duncan L1-92.
117086CIP <- 2005, J.P. Carlier, Inst. Pasteur, Paris, France: strain AIP 10223 <- DSM <- S. Duncan: strain L1-92

doi: 10.13145/bacdive6339.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Clostridia
  • order: Eubacteriales
  • family: Lachnospiraceae
  • genus: Anaerostipes
  • species: Anaerostipes caccae
  • full scientific name: Anaerostipes caccae Schwiertz et al. 2002

@ref: 5469

domain: Bacteria

phylum: Firmicutes

class: Clostridia

order: Clostridiales

family: Lachnospiraceae

genus: Anaerostipes

species: Anaerostipes caccae

full scientific name: Anaerostipes caccae Schwiertz et al. 2002

strain designation: L1-92

type strain: yes

Morphology

cell morphology

@refgram stainconfidencecell shapemotility
69480positive100
117086positiverod-shapedno

colony morphology

@refincubation period
577151 day
117086

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
5469PYG MEDIUM (MODIFIED) (DSMZ Medium 104)yeshttps://mediadive.dsmz.de/medium/104Name: PYG MEDIUM (modified) (DSMZ Medium 104) Composition: Yeast extract 10.0 g/l Peptone 5.0 g/l Trypticase peptone 5.0 g/l Beef extract 5.0 g/l Glucose 5.0 g/l L-Cysteine HCl x H2O 0.5 g/l NaHCO3 0.4 g/l NaCl 0.08 g/l K2HPO4 0.04 g/l KH2PO4 0.04 g/l MgSO4 x 7 H2O 0.02 g/l CaCl2 x 2 H2O 0.01 g/l Hemin 0.005 g/l Ethanol 0.0038 g/l Resazurin 0.001 g/l Tween 80 Vitamin K1 NaOH Distilled water
34329MEDIUM 20 - for Anaerobic bacteriayesAgar (15.000 g);Glucose (5.000 g);Yeast extract (20.000 g);Tryptone (30.000 g);Cysteine hydrochloride (0.500 g);distilled water (1000.000 ml);Hemin solution -M00149 (25.000 ml)
117086CIP Medium 20yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=20

culture temp

@refgrowthtypetemperaturerange
5469positivegrowth37mesophilic
34329positivegrowth37mesophilic
57715positivegrowth37mesophilic
67770positivegrowth37mesophilic

Physiology and metabolism

oxygen tolerance

@refoxygen toleranceconfidence
5469anaerobe
57715anaerobe
69480anaerobe99.999
117086anaerobe

spore formation

  • @ref: 69480
  • spore formation: no
  • confidence: 96.491

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
11708617632nitrate-reduction
11708616301nitrite-reduction
6838016199urea-hydrolysis
6838029016arginine-hydrolysis
6838016024D-mannose-fermentation
6838016634raffinose-fermentation
6838029985L-glutamate-degradation
6838017632nitrate-reduction
6838027897tryptophan-energy source

metabolite production

@refChebi-IDmetaboliteproduction
11708635581indoleno
6838035581indoleno

metabolite tests

  • @ref: 68380
  • Chebi-ID: 35581
  • metabolite: indole
  • indole test: -

enzymes

@refvalueactivityec
68382alpha-fucosidase-3.2.1.51
68382alpha-mannosidase-3.2.1.24
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382beta-glucosidase-3.2.1.21
68382alpha-glucosidase-3.2.1.20
68382beta-glucuronidase-3.2.1.31
68382beta-galactosidase-3.2.1.23
68382alpha-galactosidase-3.2.1.22
68382naphthol-AS-BI-phosphohydrolase+
68382acid phosphatase+3.1.3.2
68382alpha-chymotrypsin-3.4.21.1
68382trypsin-3.4.21.4
68382cystine arylamidase-3.4.11.3
68382valine arylamidase-
68382leucine arylamidase-3.4.11.1
68382lipase (C 14)-
68382esterase lipase (C 8)-
68382esterase (C 4)-
68382alkaline phosphatase-3.1.3.1
68380serine arylamidase-
68380glutamyl-glutamate arylamidase-
68380histidine arylamidase-
68380glycin arylamidase-
68380alanine arylamidase-3.4.11.2
68380tyrosine arylamidase-
68380pyrrolidonyl arylamidase-3.4.19.3
68380leucine arylamidase-3.4.11.1
68380phenylalanine arylamidase-
68380leucyl glycin arylamidase-3.4.11.1
68380proline-arylamidase-3.4.11.5
68380L-arginine arylamidase-
68380alkaline phosphatase-3.1.3.1
68380tryptophan deaminase-4.1.99.1
68380alpha-fucosidase-3.2.1.51
68380glutamate decarboxylase-4.1.1.15
68380N-acetyl-beta-glucosaminidase-3.2.1.52
68380beta-glucuronidase-3.2.1.31
68380alpha-arabinosidase-3.2.1.55
68380beta-glucosidase-3.2.1.21
68380alpha-glucosidase-3.2.1.20
68380beta-Galactosidase 6-phosphate-
68380beta-galactosidase-3.2.1.23
68380arginine dihydrolase-3.5.3.6
68380urease-3.5.1.5
117086oxidase-
117086alcohol dehydrogenase-1.1.1.1
117086catalase-1.11.1.6
117086lysine decarboxylase-4.1.1.18
117086ornithine decarboxylase-4.1.1.17
117086urease-3.5.1.5

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
117086----------++--------

API rID32A

@refUREADH Argalpha GALbeta GALbeta GPalpha GLUbeta GLUalpha ARAbeta GURbeta NAGMNERAFGDCalpha FUCNITINDPALArgAProALGAPheALeuAPyrATyrAAlaAGlyAHisAGGASerA
5469--+/---------------------------
5469--+/--------------------------
5469--+/---------------------------

Isolation, sampling and environmental information

isolation

@refsample typecountryorigin.countrycontinent
5469human faecesUnited KingdomGBREurope
57715Human fecesUnited KingdomGBREurope
67770Human feces
117086Human, FaecesUnited KingdomGBREurope

isolation source categories

Cat1Cat2Cat3
#Host#Human
#Host Body Product#Gastrointestinal tract#Feces (Stool)

taxonmaps

  • @ref: 69479
  • File name: preview.99_4472.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_33;96_2237;97_2708;98_3361;99_4472&stattab=map
  • Last taxonomy: Anaerostipes caccae subclade
  • 16S sequence: AJ270487
  • Sequence Identity:
  • Total samples: 76678
  • soil counts: 321
  • aquatic counts: 980
  • animal counts: 75194
  • plant counts: 183

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
54691Risk group (German classification)
1170861Risk group (French classification)

Sequence information

16S sequences

  • @ref: 5469
  • description: Butyrate-producing bacterium L1-92 16S rRNA gene
  • accession: AJ270487
  • length: 1456
  • database: ena
  • NCBI tax ID: 105841

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Anaerostipes caccae L1-92 DSM 14662GCA_014131675completencbi411490
66792Anaerostipes caccae L1-92 JCM 13470GCA_014467075completencbi411490
66792Anaerostipes caccae DSM 14662411490.6wgspatric411490
66792Anaerostipes caccae DSM 14662 strain Anaerostipes caccae 81B4411490.34wgspatric411490
66792Anaerostipes caccae strain DSM 14662105841.35completepatric105841
66792Anaerostipes caccae strain JCM 13470105841.45completepatric105841
66792Anaerostipes caccae L1-92641736227draftimg411490
67770Anaerostipes caccae L1-92 DSM 14662GCA_000154305scaffoldncbi411490
67770Anaerostipes caccae L1-92 NCTC11032GCA_900624715contigncbi411490
66792Anaerostipes caccae L1-92 DSM 14662GCA_025146005completencbi411490
66792Anaerostipes caccae L1-92 strain DSM 14662411490.43completepatric411490

GC content

@refGC-contentmethod
546946.0high performance liquid chromatography (HPLC)
6777046high performance liquid chromatography (HPLC)

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingyes62no
motileno85.371no
flagellatedno88.862no
gram-positiveyes87.012no
anaerobicyes98.661no
aerobicno98.448no
halophileno81.147no
spore-formingyes60.014no
thermophileno97.848yes
glucose-utilyes92.297no
glucose-fermentyes67.701no

External links

@ref: 5469

culture collection no.: DSM 14662, CIP 108612, NCIMB 13811, CCUG 47493, JCM 13470, KCTC 15019, AIP 10223

straininfo link

  • @ref: 75813
  • straininfo: 100506

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny12086188Anaerostipes caccae gen. nov., sp. nov., a new saccharolytic, acetate-utilising, butyrate-producing bacterium from human faeces.Schwiertz A, Hold GL, Duncan SH, Gruhl B, Collins MD, Lawson PA, Flint HJ, Blaut MSyst Appl Microbiol10.1078/0723-2020-000962002Acetates/metabolism, Base Sequence, Butyric Acid/metabolism, Feces/*microbiology, Gram-Positive Asporogenous Rods/enzymology/genetics/*isolation & purification, Humans, Molecular Sequence Data, Phenotype, Phylogeny, RNA, Ribosomal, 16S/analysis, Species Specificity, Sucrose/metabolismMetabolism
Metabolism16672507Two routes of metabolic cross-feeding between Bifidobacterium adolescentis and butyrate-producing anaerobes from the human gut.Belenguer A, Duncan SH, Calder AG, Holtrop G, Louis P, Lobley GE, Flint HJAppl Environ Microbiol10.1128/AEM.72.5.3593-3599.20062006Acetates/metabolism, Bacteria, Anaerobic/growth & development/*metabolism, Bifidobacterium/growth & development/*metabolism, Butyrates/*metabolism, Culture Media, Digestive System/*microbiology, Ecosystem, Fatty Acids/metabolism, Fructose, Humans, Lactates/metabolism, Oligosaccharides/metabolismCultivation
Metabolism17056678Cross-feeding between Bifidobacterium longum BB536 and acetate-converting, butyrate-producing colon bacteria during growth on oligofructose.Falony G, Vlachou A, Verbrugghe K, De Vuyst LAppl Environ Microbiol10.1128/AEM.01296-062006Acetates/*metabolism, Bacteria, Anaerobic/classification/growth & development/*metabolism, Bifidobacterium/growth & development/*metabolism, Butyrates/*metabolism, Colon/*microbiology, Culture Media, Ecosystem, Fermentation, Fructose, Humans, Oligosaccharides/*metabolism, *ProbioticsCultivation
Metabolism19633122In vitro kinetics of prebiotic inulin-type fructan fermentation by butyrate-producing colon bacteria: implementation of online gas chromatography for quantitative analysis of carbon dioxide and hydrogen gas production.Falony G, Verschaeren A, De Bruycker F, De Preter V, Verbeke K, Leroy F, De Vuyst LAppl Environ Microbiol10.1128/AEM.00876-092009Butyrates/metabolism, Carbon Dioxide/*analysis, Chromatography, Gas/*methods, Colon/*microbiology, Fermentation, Fructans/*metabolism, Gram-Positive Bacteria/growth & development/isolation & purification/*metabolism, Hydrogen/*analysis, Lactic Acid/metabolismEnzymology
Phylogeny19666785Anaerostipes butyraticus sp. nov., an anaerobic, butyrate-producing bacterium from Clostridium cluster XIVa isolated from broiler chicken caecal content, and emended description of the genus Anaerostipes.Eeckhaut V, Van Immerseel F, Pasmans F, De Brandt E, Haesebrouck F, Ducatelle R, Vandamme PInt J Syst Evol Microbiol10.1099/ijs.0.015289-02009Anaerobiosis, Animals, Bacterial Typing Techniques, Belgium, Butyrates/*metabolism, Cecum/*microbiology, Chickens/*microbiology, DNA, Bacterial/analysis, DNA, Ribosomal/analysis, Genes, rRNA, Genotype, Gram-Positive Asporogenous Rods/*classification/genetics/*isolation & purification/physiology, Molecular Sequence Data, Phenotype, Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Species SpecificityGenetics
Phylogeny22982042Anaerostipes hadrus comb. nov., a dominant species within the human colonic microbiota; reclassification of Eubacterium hadrum Moore et al. 1976.Allen-Vercoe E, Daigneault M, White A, Panaccione R, Duncan SH, Flint HJ, O'Neal L, Lawson PAAnaerobe10.1016/j.anaerobe.2012.09.0022012Bacterial Typing Techniques, Butyrates/metabolism, Cluster Analysis, Colon/*microbiology, DNA, Bacterial/chemistry/genetics, DNA, Ribosomal/chemistry/genetics, Female, Genotype, Gram-Positive Bacteria/*classification/genetics/*isolation & purification/physiology, Humans, Middle Aged, Molecular Sequence Data, Phenotype, Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNAGenetics
Phylogeny24215821Anaerostipes rhamnosivorans sp. nov., a human intestinal, butyrate-forming bacterium.Bui TPN, de Vos WM, Plugge CMInt J Syst Evol Microbiol10.1099/ijs.0.055061-02013Base Composition, Butyrates/*metabolism, DNA, Bacterial/genetics, Fatty Acids/chemistry, Gram-Positive Endospore-Forming Rods/*classification/genetics/isolation & purification, Humans, Infant, Intestines/*microbiology, Molecular Sequence Data, Netherlands, Nucleic Acid Hybridization, *Phylogeny, RNA, Ribosomal, 16S/geneticsPathogenicity
Metabolism27810444Lactate- and acetate-based cross-feeding interactions between selected strains of lactobacilli, bifidobacteria and colon bacteria in the presence of inulin-type fructans.Moens F, Verce M, De Vuyst LInt J Food Microbiol10.1016/j.ijfoodmicro.2016.10.0192016Acetates/*metabolism, Bifidobacterium/*metabolism, Butyric Acid/metabolism, Colon/*microbiology, Fermentation, Fructose/metabolism, Humans, Inulin/*metabolism, Lactic Acid/*metabolism, Lactobacillus/*metabolism, Oligosaccharides/metabolismPathogenicity
Genetics33888495Complete Genome Sequence of Anaerostipes caccae Strain L1-92(T), a Butyrate-Producing Bacterium Isolated from Human Feces.Morinaga K, Kusada H, Watanabe M, Tamaki HMicrobiol Resour Announc10.1128/MRA.00056-212021Phylogeny
Phylogeny34491487Description of Anaerostipes faecalis sp. nov., a new segmented filamentous bacterium isolated from swine faeces.Choi SH, Choi JY, Park JE, Kim JS, Kang SW, Lee J, Lee MK, Lee JS, Lee JH, Jung H, Hur TY, Kim HB, Lee JH, Kim JK, Hong Y, Park SHAntonie Van Leeuwenhoek10.1007/s10482-021-01646-z2021Animals, Bacterial Typing Techniques, Clostridiales, DNA, Bacterial/genetics, *Fatty Acids/analysis, Feces, *Phospholipids, Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, SwineTranscriptome

Reference

@idauthorscataloguedoi/urltitle
5469Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 14662)https://www.dsmz.de/collection/catalogue/details/culture/DSM-14662
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
34329Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/6260
57715Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 47493)https://www.ccug.se/strain?id=47493
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68380Automatically annotated from API rID32A
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
75813Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID100506.1StrainInfo: A central database for resolving microbial strain identifiers
117086Curators of the CIPCollection of Institut Pasteur (CIP 108612)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20108612