Strain identifier

BacDive ID: 6080

Type strain: Yes

Species: Salinicola salarius

Strain Designation: M27

Strain history: CIP <- 2008, DSMZ <- K.K. Kim, KAIST, Daejeon, Korea: strain M27

NCBI tax ID(s): 430457 (species)

« back to strain detail view
For citation purpose refer to the digital object identifier (doi) of the current version.
Archive
version 9.1 (current version):
version 9:
version 8.1:
version 8:
version 7.1:
version 7:
version 6:
version 5:
version 4.1:
version 4:
version 3:
version 2.1:
version 2:
version 1:
version 9.1 (current version)

General

@ref: 7265

BacDive-ID: 6080

DSM-Number: 18044

keywords: genome sequence, 16S sequence, Bacteria, aerobe, Gram-negative, motile, rod-shaped

description: Salinicola salarius M27 is an aerobe, Gram-negative, motile bacterium that was isolated from seawater.

NCBI tax id

  • NCBI tax id: 430457
  • Matching level: species

strain history

@refhistory
7265<- K. K. Kim, KAIST
67771<- ST Lee, KAIST
67772Halomonas salaria Kim et al. 2007 - homotypic synonym, validly published
122361CIP <- 2008, DSMZ <- K.K. Kim, KAIST, Daejeon, Korea: strain M27

doi: 10.13145/bacdive6080.20240916.9.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Gammaproteobacteria
  • order: Oceanospirillales
  • family: Halomonadaceae
  • genus: Salinicola
  • species: Salinicola salarius
  • full scientific name: Salinicola salarius (Kim et al. 2007) de la Haba et al. 2010

  • synonyms

    • @ref: 20215
    • synonym: Halomonas salaria

@ref: 7265

domain: Bacteria

phylum: Proteobacteria

class: Gammaproteobacteria

order: Oceanospirillales

family: Halomonadaceae

genus: Salinicola

species: Salinicola salarius

full scientific name: Salinicola salarius (Kim et al. 2007) de la Haba et al. 2010

strain designation: M27

type strain: yes

Morphology

cell morphology

@refgram staincell lengthcell widthcell shapemotilityconfidence
31987negative1.3-1.7 µm0.8-0.9 µmrod-shapedyes
67771negative
122361negativerod-shapedno
69480negative99.5

pigmentation

  • @ref: 31987
  • production: yes

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
7265R2A MEDIUM (DSMZ Medium 830)yeshttps://mediadive.dsmz.de/medium/830Name: R2A MEDIUM (DSMZ Medium 830; with strain-specific modifications) Composition: NaCl 100.0 g/l Agar 15.0 g/l Yeast extract 0.5 g/l Proteose peptone 0.5 g/l Casamino acids 0.5 g/l Glucose 0.5 g/l Starch 0.5 g/l Na-pyruvate 0.3 g/l K2HPO4 0.3 g/l MgSO4 x 7 H2O 0.05 g/l Distilled water
37869MEDIUM 566- Reasoner's 2A agar for Flavobacterium micromatiyesDistilled water make up to (1000.000 ml);R2A agar (18.200 g)
122361CIP Medium 566yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=566

culture temp

@refgrowthtypetemperature
7265positivegrowth28
31987positivegrowth10-45
31987positiveoptimum25-30
37869positivegrowth25
67771positivegrowth28
67772positiveoptimum25-30
67772positiveminimum10
67772positivemaximum45

culture pH

@refabilitytypepHPH range
31987positivegrowth05-10alkaliphile
31987positiveoptimum07-08
67772positiveoptimum7.0-8.0
67772positivemaximum10.0
67772positiveminimum5.0

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
31987aerobe
67771aerobe
122361obligate aerobe

spore formation

@refspore formationconfidence
31987no
69481no100

halophily

@refsaltgrowthtested relationconcentration
31987NaClpositivegrowth0-25 %
31987NaClpositiveoptimum10-20 %

observation

  • @ref: 67771
  • observation: quinones: Q-9

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
31987168082-dehydro-D-gluconate+carbon source
31987581435-dehydro-D-gluconate+carbon source
3198730089acetate+carbon source
3198722599arabinose+carbon source
3198716947citrate+carbon source
3198733984fucose+carbon source
3198717234glucose+carbon source
3198724996lactate+carbon source
3198725115malate+carbon source
3198717306maltose+carbon source
3198737684mannose+carbon source
3198726271proline+carbon source
3198717272propionate+carbon source
3198733942ribose+carbon source
3198717822serine+carbon source
3198731011valerate+carbon source
3198717632nitrate+reduction
68371Potassium 2-ketogluconate-builds acid from
6837118403L-arabitol-builds acid from
6837118287L-fucose-builds acid from
6837116443D-tagatose-builds acid from
6837132528turanose-builds acid from
6837128066gentiobiose-builds acid from
6837117151xylitol-builds acid from
6837128087glycogen-builds acid from
6837128017starch-builds acid from
6837116634raffinose-builds acid from
683716731melezitose-builds acid from
6837115443inulin-builds acid from
6837127082trehalose-builds acid from
6837117992sucrose-builds acid from
6837128053melibiose-builds acid from
6837117716lactose-builds acid from
6837117057cellobiose-builds acid from
6837117814salicin-builds acid from
683714853esculin-builds acid from
6837118305arbutin-builds acid from
6837127613amygdalin-builds acid from
6837159640N-acetylglucosamine-builds acid from
68371320061methyl alpha-D-glucopyranoside-builds acid from
6837143943methyl alpha-D-mannoside-builds acid from
6837117924D-sorbitol-builds acid from
6837116899D-mannitol-builds acid from
6837117268myo-inositol-builds acid from
6837116813galactitol-builds acid from
6837162345L-rhamnose-builds acid from
6837117266L-sorbose-builds acid from
6837174863methyl beta-D-xylopyranoside-builds acid from
6837115963ribitol-builds acid from
6837165328L-xylose-builds acid from
6837116988D-ribose-builds acid from
6837117108D-arabinose-builds acid from
6837117113erythritol-builds acid from
6837117754glycerol-builds acid from
12236117632nitrate+reduction
12236116301nitrite-reduction

metabolite production

  • @ref: 122361
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

enzymes

@refvalueactivityec
31987catalase+1.11.1.6
31987cytochrome oxidase+1.9.3.1
68382alpha-fucosidase-3.2.1.51
68382alpha-mannosidase-3.2.1.24
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382beta-glucosidase-3.2.1.21
68382alpha-glucosidase-3.2.1.20
68382beta-glucuronidase-3.2.1.31
68382beta-galactosidase-3.2.1.23
68382alpha-galactosidase-3.2.1.22
68382naphthol-AS-BI-phosphohydrolase+
68382acid phosphatase+3.1.3.2
68382alpha-chymotrypsin-3.4.21.1
68382trypsin-3.4.21.4
68382cystine arylamidase-3.4.11.3
68382valine arylamidase+
68382leucine arylamidase+3.4.11.1
68382lipase (C 14)-
68382esterase lipase (C 8)-
68382esterase (C 4)-
68382alkaline phosphatase+3.1.3.1
122361oxidase+
122361catalase+1.11.1.6
122361urease+3.5.1.5

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
122361-+---++---++--------

API 50CHac

@refGLYERYDARALARARIBDXYLLXYLADOMDXGALGLUFRUMNESBERHADULINOMANSORMDMMDGNAGAMYARBESCSALCELMALLACMELSACTREINUMLZRAFAMDGLYGXLTGENTURLYXTAGDFUCLFUCDARLLARLGNT2KG5KG
122361---+/--+/----+/-+/-+/-+/---------------+/-------------+/--+/--+/--+/--+/-

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinentsampling date
7265seawaterAnmyeondoRepublic of KoreaKORAsia
67771From seawaterRepublic of KoreaKORAsia
67772Sea waterSouth Chungcheong Province, AnmyeondoRepublic of KoreaKORAsia2006
122361Environment, Saline waterAnmyeondoRepublic of KoreaKORAsia

isolation source categories

  • Cat1: #Environmental
  • Cat2: #Aquatic
  • Cat3: #Marine

taxonmaps

  • @ref: 69479
  • File name: preview.99_6236.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_48;96_2686;97_3652;98_4620;99_6236&stattab=map
  • Last taxonomy: Salinicola
  • 16S sequence: AM229316
  • Sequence Identity:
  • Total samples: 1743
  • soil counts: 139
  • aquatic counts: 1061
  • animal counts: 434
  • plant counts: 109

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
72651Risk group (German classification)
677721
1223611Risk group (French classification)

Sequence information

16S sequences

  • @ref: 7265
  • description: Halomonas salaria partial 16S rRNA gene, type strain M27T
  • accession: AM229316
  • length: 1492
  • database: nuccore
  • NCBI tax ID: 430457

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Salinicola salarius DSM 18044GCA_003206135scaffoldncbi430457
66792Salinicola salarius strain DSM 18044430457.5wgspatric430457
66792Halomonas salaria DSM 180442900689798draftimg430457
67772Salinicola salarius 1A01339GCA_002179555contigncbi430457

GC content

@refGC-contentmethod
726558.8high performance liquid chromatography (HPLC)
3198758.8-60.1
6777158.8

Genome-based predictions

predictions

@reftraitmodeldescriptionpredictionconfidencetraining_data
69481spore-formingspore-formingAbility to form endo- or exosporesno100no
69480gram-positivegram-positivePositive reaction to Gram-stainingno99.5yes
69480anaerobicanaerobicAbility to grow under anoxygenic conditions (including facultative anaerobes)no98.474yes
69480aerobicaerobicAbility to grow under oxygenic conditions (including facultative aerobes)yes84.172no
69480spore-formingspore-formingAbility to form endo- or exosporesno88.385yes
69480thermophilicthermophileAbility to grow at temperatures above or equal to 45°Cno97.978yes
69480flagellatedmotile2+Ability to perform flagellated movementyes79.327no

External links

@ref: 7265

culture collection no.: DSM 18044, CIP 109895, KCTC 12664, UCCCB106

straininfo link

  • @ref: 75567
  • straininfo: 302759

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny17392185Halomonas gomseomensis sp. nov., Halomonas janggokensis sp. nov., Halomonas salaria sp. nov. and Halomonas denitrificans sp. nov., moderately halophilic bacteria isolated from saline water.Kim KK, Jin L, Yang HC, Lee STInt J Syst Evol Microbiol10.1099/ijs.0.64767-02007DNA, Bacterial/genetics, DNA, Ribosomal/genetics, Fatty Acids/analysis, Halomonas/*classification/genetics/isolation & purification/physiology, Molecular Sequence Data, Phylogeny, RNA, Ribosomal, 16S/genetics, Sodium Chloride, *Water MicrobiologyGenetics
Phylogeny19661506Taxonomic study of the genus Salinicola: transfer of Halomonas salaria and Chromohalobacter salarius to the genus Salinicola as Salinicola salarius comb. nov. and Salinicola halophilus nom. nov., respectively.de la Haba RR, Sanchez-Porro C, Marquez MC, Ventosa AInt J Syst Evol Microbiol10.1099/ijs.0.014480-02009Algorithms, Bacterial Typing Techniques, DNA, Ribosomal/analysis/genetics, Fatty Acids/analysis, Genes, rRNA, Halomonadaceae/*classification/genetics/physiology, Halomonas/*classification/genetics/physiology, Molecular Sequence Data, Nucleic Acid Hybridization, Phenotype, Phylogeny, RNA, Ribosomal, 16S/genetics, RNA, Ribosomal, 23S/genetics, Sequence Analysis, DNA, Species SpecificityGenetics
Phylogeny23609050Salinicola peritrichatus sp. nov., isolated from deep-sea sediment.Huo YY, Meng FX, Xu L, Wang CS, Xu XWAntonie Van Leeuwenhoek10.1007/s10482-013-9925-12013Anti-Bacterial Agents/pharmacology, Base Composition, Base Sequence, DNA, Bacterial/genetics, Fatty Acids/analysis, Geologic Sediments/*microbiology, Halomonadaceae/classification/drug effects/genetics/growth & development/*isolation & purification/metabolism/ultrastructure, Hydrogen-Ion Concentration, Lipids/analysis, Microbial Sensitivity Tests, Molecular Sequence Data, Pacific Ocean, Phenotype, Phylogeny, Quinones/analysis, RNA, Bacterial/genetics, RNA, Ribosomal, 16S/genetics, Seawater/*microbiology, Sequence Alignment, Sequence Homology, Nucleic Acid, Species Specificity, Temperature, *Water MicrobiologyGenetics

Reference

@idauthorscataloguedoi/urltitleID_cross_referencepubmed
7265Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 18044)https://www.dsmz.de/collection/catalogue/details/culture/DSM-18044
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
31987Barberan A, Caceres Velazquez H, Jones S, Fierer N.10.1128/mSphere.00237-17Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information2824128776041
37869Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/7691
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
67771Curators of the KCTChttps://kctc.kribb.re.kr/En/Kctc
67772Curators of the UCCCBhttps://ucccb.uc.pt/
68371Automatically annotated from API 50CH acid
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch10.21203/rs.3.rs-2527258/v1Predictions based on the model GenomeNet Sporulation v. 1
75567Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID302759.1StrainInfo: A central database for resolving microbial strain identifiers
122361Curators of the CIPCollection of Institut Pasteur (CIP 109895)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20109895