Strain identifier

BacDive ID: 6026

Type strain: Yes

Species: Halomonas eurihalina

Strain Designation: F9-6

Strain history: CIP <- 1999, ATCC <- E. Quesada: strain F9-6

NCBI tax ID(s): 42566 (species)

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General

@ref: 2309

BacDive-ID: 6026

DSM-Number: 5720

keywords: 16S sequence, Bacteria, obligate aerobe, mesophilic, Gram-negative, rod-shaped

description: Halomonas eurihalina F9-6 is an obligate aerobe, mesophilic, Gram-negative bacterium that was isolated from saline soil.

NCBI tax id

  • NCBI tax id: 42566
  • Matching level: species

strain history

@refhistory
2309<- Univ. Granada, F9-6
119138CIP <- 1999, ATCC <- E. Quesada: strain F9-6

doi: 10.13145/bacdive6026.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Gammaproteobacteria
  • order: Oceanospirillales
  • family: Halomonadaceae
  • genus: Halomonas
  • species: Halomonas eurihalina
  • full scientific name: Halomonas eurihalina (Quesada et al. 1990) Mellado et al. 1995

  • synonyms

    • @ref: 20215
    • synonym: Volcaniella eurihalina

@ref: 2309

domain: Bacteria

phylum: Proteobacteria

class: Gammaproteobacteria

order: Oceanospirillales

family: Halomonadaceae

genus: Halomonas

species: Halomonas eurihalina

full scientific name: Halomonas eurihalina (Quesada et al. 1990) Mellado et al. 1995

strain designation: F9-6

type strain: yes

Morphology

cell morphology

  • @ref: 119138
  • gram stain: negative
  • cell shape: rod-shaped
  • motility: no

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
2309MH MEDIUM (DSMZ Medium 624)yeshttps://mediadive.dsmz.de/medium/624Name: MH MEDIUM (DSMZ Medium 624) Composition: NaCl 60.7 g/l MgCl2 x 6 H2O 15.0 g/l Yeast extract 10.0 g/l MgSO4 x 7 H2O 7.4 g/l Proteose peptone no. 3 5.0 g/l KCl 1.5 g/l Glucose 1.0 g/l CaCl2 0.27 g/l NaHCO3 0.045 g/l NaBr 0.019 g/l Distilled water
39468MEDIUM 318 - for Halomonas eurihalinayesSodium hydrogen carbonate (0.045 g);Distilled water make up to (1000.000 ml);Sodium chloride (60.700 g);Potassium chloride (1.500 g);Magnesium chloride hexahydrate(15.000 g);Magnesium sulphate heptahydrate (7.400 g);Calcium chloride dihydrate (0.270 g);Ag
119138CIP Medium 318yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=318

culture temp

@refgrowthtypetemperaturerange
2309positivegrowth30-35mesophilic
39468positivegrowth30mesophilic
119138positivegrowth5-37
119138nogrowth41thermophilic
119138nogrowth45thermophilic

Physiology and metabolism

oxygen tolerance

  • @ref: 119138
  • oxygen tolerance: obligate aerobe

halophily

@refsaltgrowthtested relationconcentration
119138NaClpositivegrowth2-10 %
119138NaClnogrowth0 %

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
11913816947citrate-carbon source
1191384853esculin-hydrolysis
119138606565hippurate-hydrolysis
11913817632nitrate+reduction
11913816301nitrite-reduction
11913815792malonate-assimilation
11913817632nitrate-respiration

antibiotic resistance

  • @ref: 119138
  • metabolite: 0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)
  • is antibiotic: yes
  • is sensitive: no
  • is resistant: yes

metabolite production

  • @ref: 119138
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

enzymes

@refvalueactivityec
68382alpha-fucosidase-3.2.1.51
68382alpha-mannosidase-3.2.1.24
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382beta-glucosidase-3.2.1.21
68382alpha-glucosidase-3.2.1.20
68382beta-glucuronidase-3.2.1.31
68382beta-galactosidase-3.2.1.23
68382alpha-galactosidase-3.2.1.22
68382naphthol-AS-BI-phosphohydrolase+
68382acid phosphatase-3.1.3.2
68382alpha-chymotrypsin-3.4.21.1
68382trypsin-3.4.21.4
68382cystine arylamidase-3.4.11.3
68382valine arylamidase+
68382leucine arylamidase+3.4.11.1
68382lipase (C 14)-
68382esterase lipase (C 8)+
68382esterase (C 4)+
68382alkaline phosphatase+3.1.3.1
119138oxidase-
119138beta-galactosidase+3.2.1.23
119138alcohol dehydrogenase-1.1.1.1
119138amylase-
119138DNase+
119138caseinase-3.4.21.50
119138catalase+1.11.1.6
119138tween esterase+
119138gamma-glutamyltransferase+2.3.2.2
119138lecithinase-
119138lipase-
119138lysine decarboxylase-4.1.1.18
119138ornithine decarboxylase-4.1.1.17
119138phenylalanine ammonia-lyase-4.3.1.24
119138protease-
119138tryptophan deaminase-
119138urease-3.5.1.5

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
119138-+++-++----+--------

API biotype100

@refGLUFRUGALTREMNESBEMELSACRAFMTEMALLACLTEMbGaMaGaCELGENMbGuESCRIBARAXYLPLERHAFUCMLZDARLLARLXLTDULTAGGLYINOMANMTLTURSORADOHBGLYXERYMDG3MDGSATMUCLTATDTATMTATDMLTLMLTCATETATETTECITGRTGAT2KG5KGTRYNAGGNTPACPATpOBEQATGTEmOBEBATPPATCMTTGEBETPCEABTHINLATCAPCYTHISSUCFUMGREGYTAVTETNTTNGLNITA3OBUAPTGTTPRODALALALASERMNTPROPTYR2KT
119138+++++--+-+++---++-+-++-+-+++---+-+++-+--+--++---+++--++-+--++-++-------++--+--+++--++---+-++++++---

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinent
2309saline soilAlicanteSpainESPEurope
119138Environment, Saline soilAlicanteSpainESPEurope

isolation source categories

Cat1Cat2Cat3
#Environmental#Terrestrial#Soil
#Condition#Saline

taxonmaps

  • @ref: 69479
  • File name: preview.99_2524.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_48;96_1361;97_1615;98_1964;99_2524&stattab=map
  • Last taxonomy: Halomonas elongata
  • 16S sequence: X87218
  • Sequence Identity:
  • Total samples: 448
  • soil counts: 71
  • aquatic counts: 234
  • animal counts: 125
  • plant counts: 18

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
23091Risk group (German classification)
1191381Risk group (French classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Halomonas eurihalina genes for 16S ribosomal RNA, internal transcribed spacer 1 and 23S ribosomal RNA, partial and complete sequence, clone: 1349AB3672311349ena42566
2309Volcaniella eurihalina 16S ribosomal RNA (16S rRNA) geneL426201490ena42566
2309H.eurihalina 16S rRNA geneX872181473ena42566

External links

@ref: 2309

culture collection no.: DSM 5720, ATCC 49336, CIP 106091

straininfo link

  • @ref: 75512
  • straininfo: 46876

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny7547290Phylogenetic inferences and taxonomic consequences of 16S ribosomal DNA sequence comparison of Chromohalobacter marismortui, Volcaniella eurihalina, and Deleya salina and reclassification of V. eurihalina as Halomonas eurihalina comb. nov.Mellado E, Moore ER, Nieto JJ, Ventosa AInt J Syst Bacteriol10.1099/00207713-45-4-7121995Base Sequence, Chromobacterium/*classification/genetics, DNA, Bacterial/*chemistry, DNA, Ribosomal/*chemistry, Gram-Negative Bacteria/*classification, Halobacterium/*classification, Molecular Sequence Data, Phylogeny, RNA, Ribosomal, 16S/*geneticsGenetics
Phylogeny16436069Isolation and characterization of Halomonas sp. strain IMPC, a p-coumaric acid-metabolizing bacterium that decarboxylates other cinnamic acids under hypersaline conditions.Abdelkafi S, Labat M, Casalot L, Chamkha M, Sayadi SFEMS Microbiol Lett10.1111/j.1574-6968.2005.00061.x2006Anti-Bacterial Agents/pharmacology, Cinnamates/*metabolism, Coumaric Acids/*metabolism, DNA, Bacterial/analysis, Decarboxylation, Halomonas/classification/drug effects/*isolation & purification/*metabolism, Propionates, *Sodium ChlorideMetabolism
Phylogeny17519342Structural elucidation of a novel phosphoglycolipid isolated from six species of Halomonas.Giordano A, Vella FM, Romano I, Gambacorta AJ Lipid Res10.1194/jlr.M700152-JLR2002007Glycolipids/*chemistry, Halomonas/*chemistry/classification, Magnetic Resonance Spectroscopy, Mass Spectrometry, Molecular Sequence Data, Phosphatidylglycerols/*chemistry, Phospholipids/*chemistry, PhylogenyGenetics

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
2309Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 5720)https://www.dsmz.de/collection/catalogue/details/culture/DSM-5720
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
39468Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/18182
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
75512Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID46876.1StrainInfo: A central database for resolving microbial strain identifiers
119138Curators of the CIPCollection of Institut Pasteur (CIP 106091)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20106091