Strain identifier
BacDive ID: 5842
Type strain: 
Species: Glycomyces lechevalierae
Strain Designation: LLR-37B-118
Strain history: DSM 44724 <-- NRRL B-16149 <-- M. P. Lechevalier LLR-37B-118.
NCBI tax ID(s): 256034 (species)
version 8.1 (current version)
General
@ref: 12042
BacDive-ID: 5842
DSM-Number: 44724
keywords: genome sequence, 16S sequence, Bacteria, aerobe, spore-forming, mesophilic, Gram-positive
description: Glycomyces lechevalierae LLR-37B-118 is an aerobe, spore-forming, mesophilic bacterium that builds an aerial mycelium and was isolated from soil.
NCBI tax id
- NCBI tax id: 256034
- Matching level: species
strain history
| @ref | history |
|---|---|
| 12042 | <- D. P. Labeda, NRRL <- IMRU; LLR-37B-118 <- M. P. Lechevalier |
| 67770 | DSM 44724 <-- NRRL B-16149 <-- M. P. Lechevalier LLR-37B-118. |
doi: 10.13145/bacdive5842.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/actinomycetota
- domain: Bacteria
- phylum: Actinomycetota
- class: Actinomycetes
- order: Glycomycetales
- family: Glycomycetaceae
- genus: Glycomyces
- species: Glycomyces lechevalierae
- full scientific name: Glycomyces lechevalierae Labeda and Kroppenstedt 2004
@ref: 12042
domain: Bacteria
phylum: Actinobacteria
class: Actinobacteria
order: Actinomycetales
family: Glycomycetaceae
genus: Glycomyces
species: Glycomyces lechevalierae
full scientific name: Glycomyces lechevalierae Labeda and Kroppenstedt 2004
strain designation: LLR-37B-118
type strain: yes
Morphology
cell morphology
- @ref: 31227
- gram stain: positive
- cell length: 0.375 µm
colony morphology
| @ref | colony color | incubation period | medium used |
|---|---|---|---|
| 20105 | Beige (1001) | 10-14 days | ISP 2 |
| 20105 | Beige (1001) | 10-14 days | ISP 3 |
| 20105 | Beige (1001) | 10-14 days | ISP 4 |
| 20105 | Beige (1001) | 10-14 days | ISP 5 |
| 20105 | 10-14 days | ISP 6 | |
| 20105 | Beige (1001) | 10-14 days | ISP 7 |
multicellular morphology
| @ref | forms multicellular complex | medium name | complex name | complex color |
|---|---|---|---|---|
| 20105 | no | ISP 2 | ||
| 20105 | yes | ISP 3 | Aerial Mycelium | White |
| 20105 | no | ISP 4 | ||
| 20105 | yes | ISP 5 | Aerial Mycelium | White |
| 20105 | no | ISP 6 | ||
| 20105 | yes | ISP 7 | Aerial Mycelium | White |
pigmentation
- @ref: 31227
- production: no
Culture and growth conditions
culture medium
| @ref | name | growth | link | composition |
|---|---|---|---|---|
| 12042 | GYM STREPTOMYCES MEDIUM (DSMZ Medium 65) | yes | https://mediadive.dsmz.de/medium/65 | Name: GYM STREPTOMYCES MEDIUM (DSMZ Medium 65) Composition: Agar 18.0 g/l Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l CaCO3 2.0 g/l Distilled water |
| 20105 | ISP 2 | yes | Name: ISP 2 / Yeast Malt Agar (5265); 5265 Composition Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l Agar 15.0 g/l Preparation: Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.0 Usage: Maintenance and Taxonomy Organisms: All Actinomycetes | |
| 20105 | ISP 3 | yes | Name: ISP 3; 5315 Composition Dog oat flakes 20.0 g/l Trace element solution (5314) 2.5 ml/l Agar 18.0 g/l Preparation: Oat flakes are cooked for 20 minutes, trace element solution and agar are added (in the case of non rolled oat flakes the suspension has to bee filtrated). Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.8 Usage: Maintenance and taxonomy (e.g. SEM As liquid medium for metabolite production) Organisms: All Actinomycetes Trace element solution 5314 Name: Trace element solution 5314; 5314 Composition CaCl2 x H2O 3.0 g/l Fe-III-citrate 1.0 g/l MnSO4 0.2 g/l ZnCl2 0.1 g/l CuSO4 x 5 H2O 0.025 g/l Sodium tetra borate 0.2 g/l CoCl2 x 6 H2O 0.004 g/l Sodium molybdate 0.01 g/l Preparation: Use double destillated water. Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Trace element solution for different media Organisms: | |
| 20105 | ISP 4 | yes | Name: ISP 4; DSM 547 Solution I: Difco soluble starch, 10.0 g. Make a paste of the starch with a small amount of cold distilled water and bring to a volume of 500 ml. Solution II: CaCO3 2.0 g K2HPO4 (anhydrous) 1.0 g MgSO4 x 7 H2O 1.0 g NaCl 1.0 g (NH4)2SO4 2.0 g Distilled water 500.0 ml Trace salt solution (see below) 1.0 ml The pH should be between 7.0 and 7.4. Do not adjust if it is within this range. Mix solutions I and II together. Add 20.0 g agar. Liquify agar by steaming at 100°C for 10 to 20 min. Trace element solution: FeSO4 x 7 H2O 0.1 g MnCl2 x 4 H2O 0.1 g ZnSO4 x 7 H2O 0.1 g Distilled water 100.0 ml | |
| 20105 | ISP 5 | yes | Name: ISP 5 (5323) Composition L-Asparagine 1.0 g/l Glycerol 10.0 g/l K2HPO4 1.0 g/l Salt solution (see preparation) 1.0 ml/l Agar 20.0 g/l Preparation: Salt solution 1.0 g FeSO4 x 7 H2O 1.0 g MnCl2 x 4 H2O 1.0 g ZNSO4 x 7 H2O in 100 ml water Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.2 Usage: Maintenance and taxonomy Organisms: All Actinomycetes | |
| 20105 | ISP 6 | yes | Name: ISP 6 (5318) Composition Peptone 15.0 g/l Proteose peptose 5.0 g/l Ferric ammonium citrate 0.5 g/l Sodium glycerophosphate 1.0 g/l Sodium thiosulfate 0.08 g/l Yeast extract 1.0 g/l Agar 15.0 g/l Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Production of melanoid pigments Organisms: All Actinomycetes | |
| 20105 | ISP 7 | yes | Name: ISP 7 (5322) Composition Glycerol 15.0 g/l L-Tyrosine 0.5 g/l L-Asparagine 1.0 g/l K2HPO4 0.5 g/l NaCl 0.5 g/l FeSO4 x 7 H2O 0.01 g/l Trace element solution 5343 1.0 ml/l Agar 20.0 Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.3 Usage: Production of melanoid pigments Organisms: All Actinomycetes | |
| 12042 | N-Z-AMINE-MEDIUM (DSMZ Medium 554) | yes | https://mediadive.dsmz.de/medium/554 | Name: N-Z-AMINE-MEDIUM (DSMZ Medium 554) Composition: Starch 20.0 g/l Agar 20.0 g/l Glucose 10.0 g/l N-Z amine 5.0 g/l Yeast extract 5.0 g/l CaCO3 1.0 g/l Distilled water |
culture temp
| @ref | growth | type | temperature | range |
|---|---|---|---|---|
| 20105 | positive | optimum | 28 | mesophilic |
| 12042 | positive | growth | 28 | mesophilic |
| 31227 | positive | growth | 15-37 | |
| 31227 | positive | optimum | 26 | mesophilic |
| 67770 | positive | growth | 28 | mesophilic |
Physiology and metabolism
oxygen tolerance
- @ref: 31227
- oxygen tolerance: aerobe
spore formation
- @ref: 31227
- spore formation: yes
halophily
- @ref: 31227
- salt: NaCl
- growth: positive
- tested relation: optimum
- concentration: 5 %
observation
- @ref: 67770
- observation: quinones: MK-10, MK-10(H2), MK-10(H4), MK-11, MK-11(H2), MK-11(H4)
metabolite utilization
| @ref | Chebi-ID | metabolite | utilization activity | kind of utilization tested |
|---|---|---|---|---|
| 20105 | 17234 | glucose | - | |
| 20105 | 22599 | arabinose | - | |
| 20105 | 17992 | sucrose | + | |
| 20105 | 18222 | xylose | - | |
| 20105 | 17268 | myo-inositol | + | |
| 20105 | 29864 | mannitol | - | |
| 20105 | 28757 | fructose | - | |
| 20105 | 26546 | rhamnose | + | |
| 20105 | 16634 | raffinose | - | |
| 20105 | 62968 | cellulose | - | |
| 31227 | 30089 | acetate | + | carbon source |
| 31227 | 15963 | ribitol | + | carbon source |
| 31227 | 22599 | arabinose | + | carbon source |
| 31227 | 17057 | cellobiose | + | carbon source |
| 31227 | 16947 | citrate | + | carbon source |
| 31227 | 23652 | dextrin | + | carbon source |
| 31227 | 28757 | fructose | + | carbon source |
| 31227 | 28260 | galactose | + | carbon source |
| 31227 | 17234 | glucose | + | carbon source |
| 31227 | 17754 | glycerol | + | carbon source |
| 31227 | 17716 | lactose | + | carbon source |
| 31227 | 25115 | malate | + | carbon source |
| 31227 | 17306 | maltose | + | carbon source |
| 31227 | 37684 | mannose | + | carbon source |
| 31227 | 28053 | melibiose | + | carbon source |
| 31227 | 17268 | myo-inositol | + | carbon source |
| 31227 | 26546 | rhamnose | + | carbon source |
| 31227 | 17814 | salicin | + | carbon source |
| 31227 | 30911 | sorbitol | + | carbon source |
| 31227 | 30031 | succinate | + | carbon source |
| 31227 | 17992 | sucrose | + | carbon source |
| 31227 | 27082 | trehalose | + | carbon source |
| 31227 | 18222 | xylose | + | carbon source |
| 31227 | 4853 | esculin | + | hydrolysis |
| 31227 | 17632 | nitrate | + | reduction |
enzymes
| @ref | value | activity | ec |
|---|---|---|---|
| 31227 | catalase | + | 1.11.1.6 |
| 68382 | alpha-fucosidase | + | 3.2.1.51 |
| 68382 | alpha-mannosidase | - | 3.2.1.24 |
| 68382 | N-acetyl-beta-glucosaminidase | - | 3.2.1.52 |
| 68382 | beta-glucosidase | + | 3.2.1.21 |
| 68382 | alpha-glucosidase | + | 3.2.1.20 |
| 68382 | beta-glucuronidase | - | 3.2.1.31 |
| 68382 | beta-galactosidase | + | 3.2.1.23 |
| 68382 | alpha-galactosidase | + | 3.2.1.22 |
| 68382 | naphthol-AS-BI-phosphohydrolase | + | |
| 68382 | acid phosphatase | + | 3.1.3.2 |
| 68382 | alpha-chymotrypsin | + | 3.4.21.1 |
| 68382 | trypsin | - | 3.4.21.4 |
| 68382 | cystine arylamidase | - | 3.4.11.3 |
| 68382 | valine arylamidase | - | |
| 68382 | leucine arylamidase | + | 3.4.11.1 |
| 68382 | lipase (C 14) | - | |
| 68382 | esterase lipase (C 8) | + | |
| 68382 | esterase (C 4) | + | |
| 68382 | alkaline phosphatase | + | 3.1.3.1 |
API zym
| @ref | Alkaline phosphatase | Esterase | Esterase Lipase | Lipase | Leucine arylamidase | Valine arylamidase | Cystine arylamidase | Trypsin | alpha- Chymotrypsin | Acid phosphatase | Naphthol-AS-BI-phosphohydrolase | alpha- Galactosidase | beta- Galactosidase | beta- Glucuronidase | alpha- Glucosidase | beta- Glucosidase | N-acetyl-beta- glucosaminidase | alpha- Mannosidase | alpha- Fucosidase |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 20105 | + | + | + | - | + | - | - | - | + | + | + | + | + | - | + | + | - | - | + |
Isolation, sampling and environmental information
isolation
| @ref | sample type | geographic location | country | origin.country | continent |
|---|---|---|---|---|---|
| 31227 | soil | ||||
| 67770 | Soil from a corn field | Greensburg, PA | USA | USA | North America |
isolation source categories
- Cat1: #Environmental
- Cat2: #Terrestrial
- Cat3: #Soil
taxonmaps
- @ref: 69479
- File name: preview.99_168704.png
- url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_344;96_3110;97_3811;98_10147;99_168704&stattab=map
- Last taxonomy: Glycomyces
- 16S sequence: AY462041
- Sequence Identity:
- Total samples: 777
- soil counts: 588
- aquatic counts: 25
- animal counts: 39
- plant counts: 125
Safety information
risk assessment
| @ref | biosafety level | biosafety level comment |
|---|---|---|
| 12042 | 1 | Risk group (German classification) |
| 20105 | 1 | German classification |
Sequence information
16S sequences
- @ref: 31227
- description: Glycomyces lechevalierae 16S ribosomal RNA gene, partial sequence
- accession: AY462041
- length: 1507
- database: nuccore
- NCBI tax ID: 256034
Genome sequences
| @ref | description | accession | assembly level | database | NCBI tax ID |
|---|---|---|---|---|---|
| 66792 | Glycomyces lechevalierae DSM 44724 | 2923443038 | draft | img | 256034 |
| 66792 | Glycomyces lechevalierae DSM 44724 | GCA_027622945 | scaffold | ncbi | 256034 |
External links
@ref: 12042
culture collection no.: DSM 44724, JCM 14912, NBRC 107746, NRRL B-16149, NBRC 104152
straininfo link
- @ref: 75323
- straininfo: 132662
literature
| topic | Pubmed-ID | title | authors | journal | DOI | year | mesh | topic2 |
|---|---|---|---|---|---|---|---|---|
| Phylogeny | 15545481 | Emended description of the genus Glycomyces and description of Glycomyces algeriensis sp. nov., Glycomyces arizonensis sp. nov. and Glycomyces lechevalierae sp. nov. | Labeda DP, Kroppenstedt RM | Int J Syst Evol Microbiol | 10.1099/ijs.0.63089-0 | 2004 | Actinomycetales/chemistry/*classification/cytology/genetics, Bacterial Typing Techniques, Carbohydrates/analysis/isolation & purification, DNA, Bacterial/chemistry/isolation & purification, DNA, Ribosomal/chemistry/isolation & purification, Genes, rRNA, Molecular Sequence Data, Phylogeny, RNA, Bacterial/genetics, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Vitamin K 2/analysis/isolation & purification | Enzymology |
| Phylogeny | 18984687 | Glycomyces endophyticus sp. nov., an endophytic actinomycete isolated from the root of Carex baccans Nees. | Qin S, Wang HB, Chen HH, Zhang YQ, Jiang CL, Xu LH, Li WJ | Int J Syst Evol Microbiol | 10.1099/ijs.0.2008/000398-0 | 2008 | Actinomycetales/*classification/genetics/isolation & purification/physiology, Bacterial Typing Techniques, Carex Plant/*microbiology, China, DNA, Bacterial/analysis, DNA, Ribosomal/analysis, Genes, rRNA, Molecular Sequence Data, Nucleic Acid Hybridization, Phenotype, Phylogeny, Plant Roots/*microbiology, Plants, Medicinal/*microbiology, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Species Specificity | Genetics |
| Phylogeny | 30465323 | Glycomyces luteolus sp. nov., a novel actinomycete isolated from rhizosphere soil of wheat (Triticum aestivum L.). | Duan L, Song W, Jiang S, Qian L, Guo X, Wang X, Zhao J, Xiang W | Antonie Van Leeuwenhoek | 10.1007/s10482-018-1200-z | 2018 | Actinobacteria/classification/genetics/*isolation & purification/metabolism, Bacterial Typing Techniques, China, DNA, Bacterial/genetics, Fatty Acids/chemistry/metabolism, Phylogeny, RNA, Ribosomal, 16S/genetics, Rhizosphere, Soil/chemistry, *Soil Microbiology, Triticum/growth & development | Metabolism |
Reference
| @id | authors | catalogue | doi/url | title | ID_cross_reference | pubmed |
|---|---|---|---|---|---|---|
| 12042 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 44724) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-44724 | |||
| 20105 | Wink, J. | http://www.dsmz.de/microorganisms/wink_pdf/DSM44724.pdf | Compendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig | |||
| 20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | |||
| 31227 | Barberan A, Caceres Velazquez H, Jones S, Fierer N. | 10.1128/mSphere.00237-17 | Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information | 27553 | 28776041 | |
| 66792 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | |||
| 67770 | Curators of the JCM | https://jcm.brc.riken.jp/en/ | ||||
| 68382 | Automatically annotated from API zym | |||||
| 69479 | João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich. | https://microbeatlas.org/ | MicrobeAtlas 1.0 beta | |||
| 75323 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID132662.1 | StrainInfo: A central database for resolving microbial strain identifiers |