Strain identifier

BacDive ID: 5646

Type strain: Yes

Species: Tenacibaculum amylolyticum

Strain Designation: C526

Strain history: CIP <- 2001, IFO

NCBI tax ID(s): 104269 (species)

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General

@ref: 5155

BacDive-ID: 5646

DSM-Number: 13766

keywords: 16S sequence, Bacteria, mesophilic, Gram-negative, motile, rod-shaped

description: Tenacibaculum amylolyticum C526 is a mesophilic, Gram-negative, motile bacterium that was isolated from green alga .

NCBI tax id

  • NCBI tax id: 104269
  • Matching level: species

strain history

@refhistory
5155<- M. Suzuki <- T. Takadera; MBIC 4355
330972001, IFO
67770IAM 15328 <-- NBRC 16310 <-- IFO 16310 <-- MBIC 4355 <-- M. Suzuki.
116354CIP <- 2001, IFO

doi: 10.13145/bacdive5646.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacteroidota
  • domain: Bacteria
  • phylum: Bacteroidota
  • class: Flavobacteriia
  • order: Flavobacteriales
  • family: Flavobacteriaceae
  • genus: Tenacibaculum
  • species: Tenacibaculum amylolyticum
  • full scientific name: Tenacibaculum amylolyticum Suzuki et al. 2001

@ref: 5155

domain: Bacteria

phylum: Bacteroidetes

class: Flavobacteriia

order: Flavobacteriales

family: Flavobacteriaceae

genus: Tenacibaculum

species: Tenacibaculum amylolyticum

full scientific name: Tenacibaculum amylolyticum Suzuki et al. 2001

strain designation: C526

type strain: yes

Morphology

cell morphology

  • @ref: 116354
  • gram stain: negative
  • cell shape: rod-shaped
  • motility: yes

pigmentation

  • @ref: 116354
  • production: no
  • name: Flexirubin

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
5155BACTO MARINE BROTH (DIFCO 2216) (DSMZ Medium 514)yeshttps://mediadive.dsmz.de/medium/514Name: BACTO MARINE BROTH (DIFCO 2216) (DSMZ Medium 514) Composition: NaCl 19.45 g/l MgCl2 5.9 g/l Bacto peptone 5.0 g/l Na2SO4 3.24 g/l CaCl2 1.8 g/l Yeast extract 1.0 g/l KCl 0.55 g/l NaHCO3 0.16 g/l Fe(III) citrate 0.1 g/l KBr 0.08 g/l SrCl2 0.034 g/l H3BO3 0.022 g/l Na2HPO4 0.008 g/l Na-silicate 0.004 g/l NaF 0.0024 g/l (NH4)NO3 0.0016 g/l Distilled water
5155MARINE AGAR (DSMZ Medium 123)yeshttps://mediadive.dsmz.de/medium/123Name: MARINE AGAR (DSMZ Medium 123) Composition: NaCl 24.0 g/l Agar 15.0 g/l MgCl2 x 6 H2O 11.0 g/l Bacto peptone 5.0 g/l Na2SO4 4.0 g/l CaCl2 x 6 H2O 2.0 g/l Yeast extract 1.0 g/l KCl 0.7 g/l KBr 0.1 g/l SrCl2 x 6 H2O 0.04 g/l H3BO3 0.03 g/l NaSiO3 x 9 H2O 0.005 g/l NaF 0.003 g/l NH4NO3 0.002 g/l Fe(III)PO4 x 4 H2O 0.001 g/l Distilled water
33097Marine agar (MA)yesDistilled water make up to (1000.000 ml);Marine agar (55.100 g)
116354CIP Medium 13yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=13

culture temp

@refgrowthtypetemperaturerange
5155positivegrowth30mesophilic
33097positivegrowth25mesophilic
67770positivegrowth25mesophilic
116354positivegrowth25-30mesophilic
116354nogrowth5psychrophilic
116354nogrowth15psychrophilic
116354nogrowth37mesophilic
116354nogrowth41thermophilic
116354nogrowth45thermophilic

Physiology and metabolism

halophily

@refsaltgrowthtested relationconcentration
116354NaClnogrowth0 %
116354NaClnogrowth2 %
116354NaClnogrowth4 %
116354NaClnogrowth6 %
116354NaClnogrowth8 %
116354NaClnogrowth10 %

observation

  • @ref: 67770
  • observation: quinones: MK-6

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
1163544853esculin+hydrolysis
116354606565hippurate+hydrolysis
11635417632nitrate+reduction
11635416301nitrite-reduction
11635415792malonate-assimilation

metabolite production

  • @ref: 116354
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

metabolite tests

@refChebi-IDmetabolitevoges-proskauer-testmethylred-test
11635415688acetoin-
11635417234glucose-

enzymes

@refvalueactivityec
68382alpha-fucosidase-3.2.1.51
68382alpha-mannosidase-3.2.1.24
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382beta-glucosidase-3.2.1.21
68382alpha-glucosidase-3.2.1.20
68382beta-glucuronidase-3.2.1.31
68382beta-galactosidase-3.2.1.23
68382alpha-galactosidase-3.2.1.22
68382naphthol-AS-BI-phosphohydrolase+
68382acid phosphatase+3.1.3.2
68382alpha-chymotrypsin-3.4.21.1
68382trypsin-3.4.21.4
68382cystine arylamidase-3.4.11.3
68382valine arylamidase+
68382leucine arylamidase+3.4.11.1
68382lipase (C 14)-
68382esterase lipase (C 8)-
68382esterase (C 4)+
68382alkaline phosphatase+3.1.3.1
116354oxidase+
116354beta-galactosidase+3.2.1.23
116354alcohol dehydrogenase-1.1.1.1
116354gelatinase+
116354caseinase+3.4.21.50
116354catalase-1.11.1.6
116354gamma-glutamyltransferase+2.3.2.2
116354lecithinase-
116354lipase-
116354lysine decarboxylase-4.1.1.18
116354ornithine decarboxylase-4.1.1.17
116354phenylalanine ammonia-lyase+4.3.1.24
116354protease+
116354tryptophan deaminase-
116354urease-3.5.1.5

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
116354-++--++---++--------

API biotype100

@refGLUFRUGALTREMNESBEMELSACRAFMTEMALLACLTEMbGaMaGaCELGENMbGuESCRIBARAXYLPLERHAFUCMLZDARLLARLXLTDULTAGGLYINOMANMTLTURSORADOHBGLYXERYMDG3MDGSATMUCLTATDTATMTATDMLTLMLTCATETATETTECITGRTGAT2KG5KGTRYNAGGNTPACPATpOBEQATGTEmOBEBATPPATCMTTGEBETPCEABTHINLATCAPCYTHISSUCFUMGREGYTAVTETNTTNGLNITA3OBUAPTGTTPRODALALALASERMNTPROPTYR2KT
116354------+---+-------+-------------------------------------------+--+--------------------+------------

Isolation, sampling and environmental information

isolation

@refsample typehost speciesgeographic locationcountryorigin.countrycontinentisolation date
5155green alga (Avrainvillea riukiuensis)Avrainvillea riukiuensisWestern Pacific Ocean, Coast of PalauPalauPLWAustralia and Oceania
67770Green algae (Avrainvillea riukiuensis)Avrainvillea riukiuensisPalauPLWAustralia and Oceania
116354Green algaePalauJapanJPNAsia1999

isolation source categories

  • Cat1: #Host
  • Cat2: #Algae
  • Cat3: #Green algae

taxonmaps

  • @ref: 69479
  • File name: preview.99_19312.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_214;96_3736;97_10809;98_14598;99_19312&stattab=map
  • Last taxonomy: Tenacibaculum amylolyticum subclade
  • 16S sequence: AB032505
  • Sequence Identity:
  • Total samples: 143
  • soil counts: 9
  • aquatic counts: 121
  • animal counts: 13

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
51551Risk group (German classification)
1163541Risk group (French classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Tenacibaculum amylolyticum gene for 16S rRNA, partial sequence, strain: NBRC 16310AB6810611447ena104269
5155Tenacibaculum amylolyticum gene for 16S rRNA, partial sequence, strain:MBIC4355AB0325051444ena104269

GC content

  • @ref: 5155
  • GC-content: 31
  • method: high performance liquid chromatography (HPLC)

External links

@ref: 5155

culture collection no.: DSM 13766, IFO 16310, NBRC 16310, MBIC 4355, JCM 21776, CIP 107214, IAM 15328

straininfo link

  • @ref: 75129
  • straininfo: 49517

literature

  • topic: Phylogeny
  • Pubmed-ID: 11594591
  • title: Phylogenetic analysis and taxonomic study of marine Cytophaga-like bacteria: proposal for Tenacibaculum gen. nov. with Tenacibaculum maritimum comb. nov. and Tenacibaculum ovolyticum comb. nov., and description of Tenacibaculum mesophilum sp. nov. and Tenacibaculum amylolyticum sp. nov.
  • authors: Suzuki M, Nakagawa Y, Harayama S, Yamamoto S
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/00207713-51-5-1639
  • year: 2001
  • mesh: Animals, Chlorophyta/microbiology, Cytophaga/chemistry/classification/genetics, DNA Gyrase/genetics, DNA, Ribosomal/analysis/genetics, Genotype, Gram-Negative Aerobic Rods and Cocci/*classification/genetics, Nucleic Acid Hybridization, Phenotype, *Phylogeny, Porifera/microbiology, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA
  • topic2: Genetics

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
5155Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 13766)https://www.dsmz.de/collection/catalogue/details/culture/DSM-13766
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
33097Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/4625
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
75129Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID49517.1StrainInfo: A central database for resolving microbial strain identifiers
116354Curators of the CIPCollection of Institut Pasteur (CIP 107214)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20107214