Name: Tenacibaculum amylolyticum C526
Creator: BacDive
License: https://creativecommons.org/licenses/by/4.0/

Strain identifier

BacDive ID: 5646

Type strain:

Species: Tenacibaculum amylolyticum

Strain Designation: C526

Culture col. no.: DSM 13766, IFO 16310, NBRC 16310, MBIC 4355, JCM 21776, CIP 107214, IAM 15328

Strain history: CIP <- 2001, IFO

NCBI tax ID(s): 104269 (species)

SI-ID 49517

DSM 13766, IFO 16310, CIP 107214, NBRC 16310, JCM 21776, IAM 15328

Special Collections

Section

When using BacDive for research please cite our paper

Tenacibaculum amylolyticum C526 is a mesophilic, Gram-negative, motile bacterium that was isolated from green alga .

Gram-negative
motile
rod-shaped
mesophilic
16S sequence
Bacteria

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	09-12-2024 (DD-MM-YYYY)
	Domain	*Bacteria*
	Phylum	*Bacteroidota*
	Class	*Flavobacteriia*
	Order	*Flavobacteriales*
	Family	*Flavobacteriaceae*
	Genus	*Tenacibaculum*
	Species	**Tenacibaculum amylolyticum**
	Full Scientific Name (LPSN)	Tenacibaculum amylolyticum Suzuki et al. 2001

Information on morphological and physiological properties Morphology

[Ref.: #116354]	Gram stain	negative

[Ref.: #116354]	Cell shape	rod-shaped

[Ref.: #116354]	Motility	yes

[Ref.: #116354]	Pigment production	no
[Ref.: #116354]	Pigment name	Flexirubin

Information on culture and growth conditions Culture and growth conditions

[Ref.: #5155]

Culture medium

BACTO MARINE BROTH (DIFCO 2216) (DSMZ Medium 514)

[Ref.: #5155]

Culture medium growth

[Ref.: #5155]

Culture medium link

Medium recipe at MediaDive

[Ref.: #5155]

Culture medium composition

expand / minimize

[Ref.: #5155]

Culture medium

MARINE AGAR (DSMZ Medium 123)

[Ref.: #5155]

Culture medium growth

[Ref.: #5155]

Culture medium link

Medium recipe at MediaDive

[Ref.: #5155]

Culture medium composition

expand / minimize

[Ref.: #33097]

Culture medium

Marine agar (MA)

[Ref.: #33097]

Culture medium growth

[Ref.: #33097]

Culture medium composition

expand / minimize

[Ref.: #116354]

Culture medium

CIP Medium 13

[Ref.: #116354]

Culture medium growth

[Ref.: #116354]

Culture medium link

Medium recipe provided by DSMZ

	Kind of temperature		Temperature
[Ref.: #5155]		growth	30 °C
[Ref.: #33097]		growth	25 °C
[Ref.: #67770]		growth	25 °C
[Ref.: #116354]		growth	25-30 °C
[Ref.: #116354]	no	growth	5 °C
[Ref.: #116354]	no	growth	15 °C
[Ref.: #116354]	no	growth	37 °C
[Ref.: #116354]	no	growth	41 °C
[Ref.: #116354]	no	growth	45 °C

Information on physiology and metabolism Physiology and metabolism

	Salt	Tested relation		Salt conc.
[Ref.: #116354]	NaCl	no	growth	0	%
[Ref.: #116354]	NaCl	no	growth	2	%
[Ref.: #116354]	NaCl	no	growth	4	%
[Ref.: #116354]	NaCl	no	growth	6	%
[Ref.: #116354]	NaCl	no	growth	8	%
[Ref.: #116354]	NaCl	no	growth	10	%

[Ref.: #67770]

Observation

quinones: MK-6

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #116354]	esculin ChEBI	+	hydrolysis
[Ref.: #116354]	hippurate ChEBI	+	hydrolysis
[Ref.: #116354]	malonate ChEBI	-	assimilation
[Ref.: #116354]	nitrate ChEBI	+	reduction
[Ref.: #116354]	nitrite ChEBI	-	reduction

	Metabolite production	Metabolite	Production
[Ref.: #116354]		indole ChEBI	no

	Physiological tests	Metabolite	Voges-Proskauer-test	Methylred-test
[Ref.: #116354]		acetoin ChEBI	-
[Ref.: #116354]		glucose ChEBI		-

	Enzyme	Enzyme activity	EC number
[Ref.: #68382]	acid phosphatase	+	3.1.3.2	* from API zym
[Ref.: #116354]	alcohol dehydrogenase	-	1.1.1.1
[Ref.: #68382]	alkaline phosphatase	+	3.1.3.1	* from API zym
[Ref.: #68382]	alpha-chymotrypsin	-	3.4.21.1	* from API zym
[Ref.: #68382]	alpha-fucosidase	-	3.2.1.51	* from API zym
[Ref.: #68382]	alpha-galactosidase	-	3.2.1.22	* from API zym
[Ref.: #68382]	alpha-glucosidase	-	3.2.1.20	* from API zym
[Ref.: #68382]	alpha-mannosidase	-	3.2.1.24	* from API zym
[Ref.: #116354]	beta-galactosidase	+	3.2.1.23
[Ref.: #68382]	beta-galactosidase	-	3.2.1.23	* from API zym
[Ref.: #68382]	beta-glucosidase	-	3.2.1.21	* from API zym
[Ref.: #68382]	beta-glucuronidase	-	3.2.1.31	* from API zym
[Ref.: #116354]	caseinase	+	3.4.21.50
[Ref.: #116354]	catalase	-	1.11.1.6
[Ref.: #68382]	cystine arylamidase	-	3.4.11.3	* from API zym
[Ref.: #68382]	esterase (C 4)	+		* from API zym
[Ref.: #68382]	esterase lipase (C 8)	-		* from API zym
[Ref.: #116354]	gamma-glutamyltransferase	+	2.3.2.2
[Ref.: #116354]	gelatinase	+
[Ref.: #116354]	lecithinase	-
[Ref.: #68382]	leucine arylamidase	+	3.4.11.1	* from API zym
[Ref.: #116354]	lipase	-
[Ref.: #68382]	lipase (C 14)	-		* from API zym
[Ref.: #116354]	lysine decarboxylase	-	4.1.1.18
[Ref.: #68382]	N-acetyl-beta-glucosaminidase	-	3.2.1.52	* from API zym
[Ref.: #68382]	naphthol-AS-BI-phosphohydrolase	+		* from API zym
[Ref.: #116354]	ornithine decarboxylase	-	4.1.1.17
[Ref.: #116354]	oxidase	+
[Ref.: #116354]	phenylalanine ammonia-lyase	+	4.3.1.24
[Ref.: #116354]	protease	+
[Ref.: #68382]	trypsin	-	3.4.21.4	* from API zym
[Ref.: #116354]	tryptophan deaminase	-
[Ref.: #116354]	urease	-	3.5.1.5
[Ref.: #68382]	valine arylamidase	+		* from API zym
	Only first 10 entries are displayed. Click here to see all.

API® Biotype100:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	ControlQ	D-glucose as carbon sourceGLU	D-fructose as carbon sourceFRU	D-galactose as carbon sourceGAL	D-trehalose as carbon sourceTRE	D-mannose as carbon sourceMNE	L-sorbose as carbon sourceSBE	D-melibiose as carbon sourceMEL	sucrose as carbon sourceSAC	D-raffinose as carbon sourceRAF	maltotriose as carbon sourceMTE	maltose as carbon sourceMAL	lactose as carbon sourceLAC	lactulose as carbon sourceLTE	1-O-methyl-beta-galactopyranoside as carbon sourceMbGa	1-O-methyl-alpha-galactopyranoside as carbon sourceMaGa	D-cellobiose as carbon sourceCEL	gentiobiose as carbon sourceGEN	1-O-methyl-beta-D-glucopyranoside as carbon sourceMbGu	esculin as carbon sourceESC	D-ribose as carbon sourceRIB	L-arabinose as carbon sourceARA	D-xylose as carbon sourceXYL	palatinose as carbon sourcePLE	L-rhamnose as carbon sourceRHA	L-fucose as carbon sourceFUC	D-melezitose as carbon sourceMLZ	D-arabitol as carbon sourceDARL	L-arabitol as carbon sourceLARL	xylitol as carbon sourceXLT	dulcitol as carbon sourceDUL	D-tagatose as carbon sourceTAG	glycerol as carbon sourceGLY	myo-inositol as carbon sourceINO	D-mannitol as carbon sourceMAN	maltitol as carbon sourceMTL	D-turanose as carbon sourceTUR	D-sorbitol as carbon sourceSOR	adonitol as carbon sourceADO	hydroxyquinoline-beta-glucuronide as carbon sourceHBG	D-lyxose as carbon sourceLYX	i-erythritol as carbon sourceERY	1-O-methyl-alpha-D-glucoside as carbon sourceMDG	3-O-methyl-D-glucose as carbon source3MDG	D-saccharate as carbon sourceSAT	mucate as carbon sourceMUC	L-tartrate as carbon sourceLTAT	D-tartrate as carbon sourceDTAT	meso-tartrate as carbon sourceMTAT	D-malate as carbon sourceDMLT	L-malate as carbon sourceLMLT	cis-aconitate as carbon sourceCATE	trans-aconitate as carbon sourceTATE	tricarballylate as carbon sourceTTE	citrate as carbon sourceCIT	D-glucuronate as carbon sourceGRT	D-galacturonate as carbon sourceGAT	2-ketogluconate as carbon source2KG	5-ketogluconate as carbon source5KG	tryptophan as carbon sourceTRY	N-acetyl-D-glucosamine as carbon sourceNAG	D-gluconate as carbon sourceGNT	phenylacetate as carbon sourcePAC	protocatechuate as carbon sourcePAT	4-hydroxybenzoate as carbon sourcepOBE	quinate as carbon sourceQAT	gentisate as carbon sourceGTE	3-hydroxybenzoate as carbon sourcemOBE	benzoate as carbon sourceBAT	3-phenylpropionate as carbon sourcePPAT	m-coumarate as carbon sourceCMT	trigonelline as carbon sourceTGE	betaine as carbon sourceBET	putrescine as carbon sourcePCE	4-aminobutyrate as carbon sourceABT	histamine as carbon sourceHIN	DL-lactate as carbon sourceLAT	caprate as carbon sourceCAP	caprylate as carbon sourceCYT	L-histidine as carbon sourceHIS	succinate as carbon sourceSUC	fumarate as carbon sourceFUM	glutarate as carbon sourceGRE	DL-glycerate as carbon sourceGYT	5-aminovalerate as carbon sourceAVT	ethanolamine as carbon sourceETN	tryptamine as carbon sourceTTN	D-glucosamine as carbon sourceGLN	itaconate as carbon sourceITA	3-hydroxybutyrate as carbon source3OBU	L-aspartate as carbon sourceAPT	L-glutamate as carbon sourceGTT	L-proline as carbon sourcePRO	D-alanine as carbon sourceDALA	L-alanine as carbon sourceLALA	L-serine as carbon sourceSER	malonate as carbon sourceMNT	propionate as carbon sourcePROP	L-tyrosine as carbon sourceTYR	2-ketoglutarate as carbon source2KT
[Ref.: #116354]	2072	not determinedn.d.	-	-	-	-	-	-	+	-	-	-	+	-	-	-	-	-	-	-	+	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	+	-	-	+	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	+	-	-	-	-	-	-	-	-	-	-	-	-

API® zym:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Control	2-naphthyl phosphateAlkaline phosphatase	2-naphthyl butyrateEsterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	2-naphthyl myristateLipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	N-benzoyl-DL-arginine-2-naphthylamideTrypsin	N-glutaryl-phenylalanine-2-naphthylamidealpha- Chymotrypsin	2-naphthyl phosphateAcid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	6-Br-2-naphthyl-alphaD-galactpyranosidealpha- Galactosidase	2-naphthyl-betaD-galactpyranosidebeta- Galactosidase	Naphthol-AS-BI-betaD-glucuronidebeta- Glucuronidase	2-naphthyl-alphaD-glucopyranosidealpha- Glucosidase	6-Br-2-naphthyl-betaD-glucopyranosidebeta- Glucosidase	1-naphthyl-N-acetyl-betaD-glucosaminideN-acetyl-beta- glucosaminidase	6-Br-2-naphthyl-alphaD-mannopyranosidealpha- Mannosidase	2-naphthyl-alphaL-fucopyranosidealpha- Fucosidase
[Ref.: #116354]	10165	-	+	+	-	-	+	+	-	-	-	+	+	-	-	-	-	-	-	-	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #5155]	Sample type/isolated from	green alga (Avrainvillea riukiuensis)
[Ref.: #5155]	Host species	Avrainvillea riukiuensis
[Ref.: #5155]	Geographic location (country and/or sea, region)	Western Pacific Ocean, Coast of Palau
[Ref.: #5155]	Country	Palau
[Ref.: #5155]	Country ISO 3 Code	PLW
[Ref.: #5155]	Continent	Australia and Oceania

[Ref.: #67770]	Sample type/isolated from	Green algae (Avrainvillea riukiuensis)
[Ref.: #67770]	Host species	Avrainvillea riukiuensis
[Ref.: #67770]	Country	Palau
[Ref.: #67770]	Country ISO 3 Code	PLW
[Ref.: #67770]	Continent	Australia and Oceania

[Ref.: #116354]	Sample type/isolated from	Green algae
[Ref.: #116354]	Geographic location (country and/or sea, region)	Palau
[Ref.: #116354]	Country	Japan
[Ref.: #116354]	Country ISO 3 Code	JPN
[Ref.: #116354]	Continent	Asia
[Ref.: #116354]	Isolation date	1999
* marker position based on {}

Isolation sources categories

#Host

#Algae

#Green algae

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence AB032505 (>99% sequence identity) for Tenacibaculum amylolyticum subclade from Microbeatlas

Aquatic Samples	121
Soil Samples	9
Animal Samples	13
Plant Samples
Total Samples	143

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #5155]	Biosafety level	1	Risk group (German classification) According to TRBA 466
[Ref.: #116354]	Biosafety level	1	Risk group (French classification)

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #5155]	Tenacibaculum amylolyticum gene for 16S rRNA, partial sequence, strain:MBIC4355	AB032505	1444	GenBank ENA	104269 tax ID
[Ref.: #124043]	Tenacibaculum amylolyticum NBRC 16310 DNA, 16S-23S rRNA intergenic spacer.	LC724055	589	GenBank ENA	104269 tax ID	*
[Ref.: #20218]	Tenacibaculum amylolyticum gene for 16S rRNA, partial sequence, strain: NBRC 16310	AB681061	1447	GenBank ENA	104269 tax ID	*

[Ref.: #5155]

GC-content

31 mol%

high performance liquid chromatography (HPLC)

Availability in culture collections External links

[Ref.: #5155]

Culture collection no.

DSM 13766, IFO 16310, NBRC 16310, MBIC 4355, JCM 21776, CIP 107214, IAM 15328

[Ref.: #75129]

SI-ID 49517

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Phylogenetic analysis and taxonomic study of marine Cytophaga-like bacteria: proposal for Tenacibaculum gen. nov. with Tenacibaculum maritimum comb. nov. and Tenacibaculum ovolyticum comb. nov., and description of Tenacibaculum mesophilum sp. nov. and Tenacibaculum amylolyticum sp. nov.	Suzuki M, Nakagawa Y, Harayama S, Yamamoto S	Int J Syst Evol Microbiol	10.1099/00207713-51-5-1639	2001	*

References

#5155

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 13766

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#20218

Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )

#33097 ; Curators of the CIP;
#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;

#68382

Automatically annotated from API zym .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#75129

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID49517.1 )

#116354 Collection of Institut Pasteur ; Curators of the CIP; CIP 107214

#124043

Isabel Schober, Julia Koblitz: Data extracted from sequence databases, automatically matched based on designation and taxonomy .

* These data were automatically processed and therefore are not curated

Change proposal

You found an error in BacDive? Please tell us about it!

Note that changes will be reviewed and judged. If your changes are legitimate, changes will occur within the next BacDive update. Only proposed changes supported by the according reference will be reviewed. The BacDive team reserves the right to reject proposed changes.

Search for species Tenacibaculum amylolyticum in external resources:
SILVA
BRENDA
PANGAEA
GBIF