Strain identifier

BacDive ID: 5570

Type strain: Yes

Species: Flavobacterium anhuiense

Strain Designation: D3

Strain history: CIP <- 2009, KCTC

NCBI tax ID(s): 459526 (species)

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General

@ref: 16396

BacDive-ID: 5570

DSM-Number: 22555

keywords: genome sequence, 16S sequence, Bacteria, aerobe, Gram-negative, motile, rod-shaped

description: Flavobacterium anhuiense D3 is an aerobe, Gram-negative, motile bacterium that was isolated from field-soil.

NCBI tax id

  • NCBI tax id: 459526
  • Matching level: species

strain history

@refhistory
16396<- KCTC <- H. Liu, Nanjing Agric. Univ., China
67770H. Liu D3.
67771<- H Liu, Nanjing Agri. Univ., China
116390CIP <- 2009, KCTC

doi: 10.13145/bacdive5570.20240916.9.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacteroidota
  • domain: Bacteria
  • phylum: Bacteroidota
  • class: Flavobacteriia
  • order: Flavobacteriales
  • family: Flavobacteriaceae
  • genus: Flavobacterium
  • species: Flavobacterium anhuiense
  • full scientific name: Flavobacterium anhuiense Liu et al. 2008

@ref: 16396

domain: Bacteria

phylum: Bacteroidetes

class: Flavobacteriia

order: Flavobacteriales

family: Flavobacteriaceae

genus: Flavobacterium

species: Flavobacterium anhuiense

full scientific name: Flavobacterium anhuiense Liu et al. 2008

strain designation: D3

type strain: yes

Morphology

cell morphology

@refgram staincell lengthcell widthcell shapemotilityflagellum arrangementconfidence
32470negative1.75 µm0.3 µmrod-shapedyesgliding
67771rod-shapedyesgliding
67771negative
116390negativerod-shapedno
69480no91.5
69480negative96.727

pigmentation

  • @ref: 32470
  • production: yes

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
16396TYB MEDIUM (DSMZ Medium 1247)yeshttps://mediadive.dsmz.de/medium/1247Name: TYB (DSMZ Medium 1247) Composition: Agar 15.0 g/l Tryptone 6.0 g/l Yeast extract 3.0 g/l NaCl 3.0 g/l Beef extract 2.0 g/l FeCl3 0.1 g/l Distilled water
116390CIP Medium 72yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=72

culture temp

@refgrowthtypetemperature
16396positivegrowth25
32470positivegrowth05-37
32470positiveoptimum27.5
67770positivegrowth30
67771positivegrowth25-30

culture pH

@refabilitytypepHPH range
32470positivegrowth04-10alkaliphile
32470positiveoptimum7

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
32470aerobe
67771aerobe
116390obligate aerobe

spore formation

@refspore formation
32470no
67771no

halophily

@refsaltgrowthtested relationconcentration
32470NaClpositivegrowth0-2 %
32470NaClpositiveoptimum0.5 %

observation

@refobservation
67770quinones: MK-6
67771quinones: MK-6

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
3247022599arabinose+carbon source
3247017057cellobiose+carbon source
3247023652dextrin+carbon source
3247028757fructose+carbon source
3247028260galactose+carbon source
3247017234glucose+carbon source
3247017716lactose+carbon source
3247017306maltose+carbon source
3247028053melibiose+carbon source
3247016634raffinose+carbon source
3247017814salicin+carbon source
3247017992sucrose+carbon source
3247017151xylitol+carbon source
3247018222xylose+carbon source
11639017632nitrate-reduction
11639016301nitrite-reduction

metabolite production

  • @ref: 116390
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

enzymes

@refvalueactivityec
32470acid phosphatase+3.1.3.2
32470alkaline phosphatase+3.1.3.1
32470catalase+1.11.1.6
68382alpha-fucosidase-3.2.1.51
68382alpha-mannosidase-3.2.1.24
68382N-acetyl-beta-glucosaminidase+3.2.1.52
68382beta-glucosidase+3.2.1.21
68382alpha-glucosidase+3.2.1.20
68382beta-glucuronidase-3.2.1.31
68382beta-galactosidase+3.2.1.23
68382alpha-galactosidase-3.2.1.22
68382naphthol-AS-BI-phosphohydrolase+
68382acid phosphatase+3.1.3.2
68382alpha-chymotrypsin-3.4.21.1
68382trypsin-3.4.21.4
68382cystine arylamidase+3.4.11.3
68382valine arylamidase+
68382leucine arylamidase+3.4.11.1
68382lipase (C 14)-
68382esterase lipase (C 8)-
68382esterase (C 4)+
68382alkaline phosphatase+3.1.3.1
116390oxidase+
116390catalase+1.11.1.6
116390urease-3.5.1.5

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
116390-++--+++--++-+-+++--

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinent
16396field-soilAnhui ProvinceChinaCHNAsia
67770Soil from Anhui ProvinceChinaCHNAsia
67771From soilAnhui provinceChinaCHNAsia
116390Environment, Soil, fieldAnhuiChinaCHNAsia

isolation source categories

Cat1Cat2Cat3
#Engineered#Agriculture#Field
#Environmental#Terrestrial#Soil

taxonmaps

  • @ref: 69479
  • File name: preview.99_301.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_66;96_207;97_226;98_255;99_301&stattab=map
  • Last taxonomy: Flavobacterium
  • 16S sequence: EU046269
  • Sequence Identity:
  • Total samples: 780
  • soil counts: 350
  • aquatic counts: 213
  • animal counts: 143
  • plant counts: 74

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
163961Risk group (German classification)
1163901Risk group (French classification)

Sequence information

16S sequences

  • @ref: 16396
  • description: Flavobacterium anhuiense strain D3 16S ribosomal RNA gene, partial sequence
  • accession: EU046269
  • length: 1398
  • database: nuccore
  • NCBI tax ID: 459526

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Flavobacterium anhuiense CGMCC 1.6859GCA_900101855scaffoldncbi459526
66792Flavobacterium anhuiense strain CGMCC 1.6859459526.10wgspatric459526
66792Flavobacterium anhuiense CGMCC 1.68592596583647draftimg459526

GC content

@refGC-contentmethod
1639631.4
6777031.4thermal denaturation, midpoint method (Tm)

Genome-based predictions

predictions

@reftraitmodeldescriptionpredictionconfidencetraining_data
69480gram-positivegram-positivePositive reaction to Gram-stainingno96.727no
69480anaerobicanaerobicAbility to grow under anoxygenic conditions (including facultative anaerobes)no98.874yes
69480aerobicaerobicAbility to grow under oxygenic conditions (including facultative aerobes)yes86.383yes
69480spore-formingspore-formingAbility to form endo- or exosporesno84.089no
69480thermophilicthermophileAbility to grow at temperatures above or equal to 45°Cno97.924no
69480flagellatedmotile2+Ability to perform flagellated movementno91.5yes

External links

@ref: 16396

culture collection no.: DSM 22555, CGMCC 1.6859, KCTC 22128, JCM 14864, CIP 109938

straininfo link

  • @ref: 75053
  • straininfo: 397181

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny18398165Flavobacterium anhuiense sp. nov., isolated from field soil.Liu H, Liu R, Yang SY, Gao WK, Zhang CX, Zhang KY, Lai RInt J Syst Evol Microbiol10.1099/ijs.0.65536-02008Base Composition, Base Sequence, China, DNA Primers/genetics, DNA, Bacterial/chemistry/genetics, Fatty Acids/analysis, Flavobacterium/chemistry/classification/*genetics/*isolation & purification, Molecular Sequence Data, Phylogeny, RNA, Bacterial/genetics, RNA, Ribosomal, 16S/genetics, Soil Microbiology, Species Specificity, Terminology as Topic, Terpenes/analysisGenetics
Phylogeny21186290Flavobacterium phragmitis sp. nov., an endophyte of reed (Phragmites australis).Liu M, Li YH, Liu Y, Zhu JN, Liu QF, Liu Y, Gu JG, Zhang XX, Li CLInt J Syst Evol Microbiol10.1099/ijs.0.027417-02010Base Composition, China, DNA, Bacterial/genetics, Endophytes/*classification/genetics/*isolation & purification/metabolism, Fatty Acids/metabolism, Flavobacterium/*classification/genetics/*isolation & purification/metabolism, Molecular Sequence Data, Phylogeny, Plant Roots/microbiology, Poaceae/*microbiology, RNA, Ribosomal, 16S/geneticsMetabolism

Reference

@idauthorscataloguedoi/urltitleID_cross_referencepubmed
16396Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 22555)https://www.dsmz.de/collection/catalogue/details/culture/DSM-22555
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
32470Barberan A, Caceres Velazquez H, Jones S, Fierer N.10.1128/mSphere.00237-17Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information2869128776041
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
67771Curators of the KCTChttps://kctc.kribb.re.kr/En/Kctc
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
75053Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID397181.1StrainInfo: A central database for resolving microbial strain identifiers
116390Curators of the CIPCollection of Institut Pasteur (CIP 109938)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20109938