Strain identifier

BacDive ID: 5327

Type strain: Yes

Species: Enterococcus sulfureus

Strain history: CIP <- 1995, DSM <- NCFB <- D. Jones <- J.O. Mundt: strain MUTK 31

NCBI tax ID(s): 1140003 (strain), 1356 (species)

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General

@ref: 2884

BacDive-ID: 5327

DSM-Number: 6905

keywords: genome sequence, 16S sequence, Bacteria, microaerophile, mesophilic, Gram-positive

description: Enterococcus sulfureus CCUG 30571 is a microaerophile, mesophilic, Gram-positive bacterium that was isolated from plant material.

NCBI tax id

NCBI tax idMatching level
1140003strain
1356species

strain history

@refhistory
2884<- NCFB <- D. Jones <- J.O. Mundt, MUTK 31
123384CIP <- 1995, DSM <- NCFB <- D. Jones <- J.O. Mundt: strain MUTK 31

doi: 10.13145/bacdive5327.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Bacilli
  • order: Lactobacillales
  • family: Enterococcaceae
  • genus: Enterococcus
  • species: Enterococcus sulfureus
  • full scientific name: Enterococcus sulfureus Martinez-Murcia and Collins 1991

@ref: 2884

domain: Bacteria

phylum: Firmicutes

class: Bacilli

order: Lactobacillales

family: Enterococcaceae

genus: Enterococcus

species: Enterococcus sulfureus

full scientific name: Enterococcus sulfureus Martinez-Murcia and Collins 1991

type strain: yes

Morphology

cell morphology

@refgram stainconfidencecell shapemotility
69480positive100
123384positiveovoid-shapedno

colony morphology

  • @ref: 2884
  • type of hemolysis: gamma
  • incubation period: 1-2 days

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
2884COLUMBIA BLOOD MEDIUM (DSMZ Medium 693)yeshttps://mediadive.dsmz.de/medium/693Name: COLUMBIA BLOOD MEDIUM (DSMZ Medium 693) Composition: Defibrinated sheep blood 50.0 g/l Columbia agar base
2884TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92)yeshttps://mediadive.dsmz.de/medium/92Name: TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92) Composition: Trypticase soy broth 30.0 g/l Agar 15.0 g/l Yeast extract 3.0 g/l Distilled water
34691MEDIUM 29- Brain heart agaryesDistilled water make up to (1000.000 ml);Brain heart infusion agar (52.000 g)
123384CIP Medium 29yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=29

culture temp

@refgrowthtypetemperaturerange
2884positivegrowth37mesophilic
34691positivegrowth37mesophilic

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
2884microaerophile
123384facultative anaerobe

spore formation

  • @ref: 69480
  • spore formation: no
  • confidence: 99.895

murein

  • @ref: 2884
  • murein short key: A11.05
  • type: A3alpha L-Lys-L-Ala2

metabolite utilization

@refmetaboliteutilization activitykind of utilization testedChebi-ID
68371Potassium 2-ketogluconate+builds acid from
68371gluconate+builds acid from24265
68371L-arabitol-builds acid from18403
68371D-arabitol-builds acid from18333
68371L-fucose-builds acid from18287
68371D-fucose-builds acid from28847
68371D-tagatose-builds acid from16443
68371D-lyxose-builds acid from62318
68371turanose+builds acid from32528
68371gentiobiose+builds acid from28066
68371xylitol-builds acid from17151
68371glycogen-builds acid from28087
68371starch+builds acid from28017
68371raffinose+builds acid from16634
68371melezitose+builds acid from6731
68371inulin-builds acid from15443
68371trehalose+builds acid from27082
68371sucrose+builds acid from17992
68371melibiose+builds acid from28053
68371lactose+builds acid from17716
68371maltose+builds acid from17306
68371cellobiose+builds acid from17057
68371salicin+builds acid from17814
68371esculin+builds acid from4853
68371arbutin+builds acid from18305
68371amygdalin+builds acid from27613
68371N-acetylglucosamine+builds acid from59640
68371methyl alpha-D-glucopyranoside+builds acid from320061
68371methyl alpha-D-mannoside-builds acid from43943
68371D-sorbitol-builds acid from17924
68371D-mannitol-builds acid from16899
68371myo-inositol-builds acid from17268
68371galactitol-builds acid from16813
68371L-rhamnose-builds acid from62345
68371L-sorbose-builds acid from17266
68371D-mannose+builds acid from16024
68371D-fructose+builds acid from15824
68371D-glucose+builds acid from17634
68371D-galactose+builds acid from12936
68371methyl beta-D-xylopyranoside-builds acid from74863
68371ribitol-builds acid from15963
68371L-xylose-builds acid from65328
68371D-xylose-builds acid from65327
68371D-ribose+builds acid from16988
68371L-arabinose-builds acid from30849
68371D-arabinose-builds acid from17108
68371erythritol-builds acid from17113
123384nitrate-reduction17632
123384nitrite-reduction16301
68381arginine-hydrolysis29016
68381D-ribose+builds acid from16988
68381D-mannitol-builds acid from16899
68381sorbitol-builds acid from30911
68381lactose+builds acid from17716
68381trehalose+builds acid from27082
68381sucrose+builds acid from17992
68381L-arabinose-builds acid from30849
68381D-arabitol-builds acid from18333
68381alpha-cyclodextrin+builds acid from40585
68381hippurate-hydrolysis606565
68381glycogen-builds acid from28087
68381pullulan-builds acid from27941
68381maltose+builds acid from17306
68381melibiose+builds acid from28053
68381melezitose+builds acid from6731
68381methyl beta-D-glucopyranoside+builds acid from320055
68381D-tagatose-builds acid from16443
68381urea-hydrolysis16199

metabolite production

@refChebi-IDmetaboliteproduction
12338435581indoleno
6838115688acetoinyes

metabolite tests

  • @ref: 68381
  • Chebi-ID: 15688
  • metabolite: acetoin
  • voges-proskauer-test: +

enzymes

@refvalueactivityec
2884catalase+1.11.1.6
2884cytochrome-c oxidase-1.9.3.1
68382alpha-fucosidase-3.2.1.51
68382alpha-mannosidase-3.2.1.24
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382beta-glucosidase+3.2.1.21
68382alpha-glucosidase+3.2.1.20
68382beta-glucuronidase-3.2.1.31
68382beta-galactosidase+3.2.1.23
68382alpha-galactosidase+3.2.1.22
68382naphthol-AS-BI-phosphohydrolase+
68382acid phosphatase+3.1.3.2
68382alpha-chymotrypsin-3.4.21.1
68382trypsin-3.4.21.4
68382cystine arylamidase-3.4.11.3
68382valine arylamidase-
68382leucine arylamidase-3.4.11.1
68382lipase (C 14)-
68382esterase lipase (C 8)+
68382esterase (C 4)+
68382alkaline phosphatase+3.1.3.1
68381urease-3.5.1.5
68381N-acetyl-beta-glucosaminidase+3.2.1.52
68381pyrrolidonyl arylamidase+3.4.19.3
68381beta-galactosidase+3.2.1.23
68381Alanyl-Phenylalanyl-Proline arylamidase-
68381alpha-galactosidase+3.2.1.22
68381beta-glucuronidase-3.2.1.31
68381beta-glucosidase+3.2.1.21
68381arginine dihydrolase-3.5.3.6
123384oxidase-
123384catalase-1.11.1.6
123384urease-3.5.1.5

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
123384-+++------++++-++---

API 50CHac

@refGLYERYDARALARARIBDXYLLXYLADOMDXGALGLUFRUMNESBERHADULINOMANSORMDMMDGNAGAMYARBESCSALCELMALLACMELSACTREINUMLZRAFAMDGLYGXLTGENTURLYXTAGDFUCLFUCDARLLARLGNT2KG5KG
123384+/----+----++++-------++++++++++++-+++--++------+++/-

API rID32STR

@refADH Argbeta GLUbeta GARbeta GURalpha GALPALRIBMANSORLACTRERAFSACLARADARLCDEXVPAPPAbeta GALPyrAbeta NAGGTAHIPGLYGPULMALMELMLZMbeta DGTAGbeta MANURE
50159-++-+-+--++-+--++-+++----++++---
2884-++-+-+--++++--++-++++---++++-+-
2884-++-+++--++++--++-++++---++++-+-

Isolation, sampling and environmental information

isolation

@refsample typecountryorigin.countrycontinent
2884plant materialUSAUSANorth America
50159PlantUSAUSANorth America
123384Plant, Material

isolation source categories

  • Cat1: #Host
  • Cat2: #Plants

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
28841Risk group (German classification)
1233842Risk group (French classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Enterococcus sulfureus strain ATCC 49903 16S ribosomal RNA gene, partial sequenceDQ4118151479ena1140003
2884Enterococcus sulfureus 16S ribosomal RNA gene, partial sequenceAF0610011498ena1140003

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Enterococcus sulfureus ATCC 49903GCA_000407605contigncbi1140003
66792Enterococcus sulfureus ATCC 49903GCA_000407025scaffoldncbi1140003
66792Enterococcus sulfureus ATCC 49903 [PRJNA206272]1140003.3wgspatric1140003
66792Enterococcus sulfureus ATCC 49903 [PRJNA206366]1140003.4wgspatric1140003
66792Enterococcus sulfureus ATCC 499032541047450draftimg1140003
66792Enterococcus sulfureus ATCC 499032545824672draftimg1140003

GC content

  • @ref: 2884
  • GC-content: 38.0
  • method: thermal denaturation, midpoint method (Tm)

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingyes52no
motileno94.801no
flagellatedno95.586no
gram-positiveyes92.405no
anaerobicno98.185no
aerobicno94.644no
halophileyes84.936no
spore-formingno89.959no
thermophileno99.608yes
glucose-utilyes91.366no
glucose-fermentyes88.121no

External links

@ref: 2884

culture collection no.: CCUG 30571, CCM 4283, NCIMB 13117, DSM 6905, ATCC 49903, CIP 104373, NCDO 2379, MUTK 31, CCUG 33313

straininfo link

  • @ref: 74816
  • straininfo: 7715

literature

  • topic: Phylogeny
  • Pubmed-ID: 26346480
  • title: Enterococcus bulliens sp. nov., a novel lactic acid bacterium isolated from camel milk.
  • authors: Kadri Z, Spitaels F, Cnockaert M, Praet J, El Farricha O, Swings J, Vandamme P
  • journal: Antonie Van Leeuwenhoek
  • DOI: 10.1007/s10482-015-0579-z
  • year: 2015
  • mesh: Animals, Base Composition, Camelus, DNA, Bacterial, Enterococcus/chemistry/*classification/genetics/isolation & purification/*metabolism, *Food Microbiology, Lactic Acid/*biosynthesis, Milk/*microbiology, Phenotype, Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
  • topic2: Biotechnology

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
2884Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 6905)https://www.dsmz.de/collection/catalogue/details/culture/DSM-6905
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
34691Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/16274
50159Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 30571)https://www.ccug.se/strain?id=30571
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
66794Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg10.1093/nar/gkaa1025BRENDA, the ELIXIR core data resource in 2021: new developments and updatesNucleic Acids Res. 49: D498-D508 202033211880
68371Automatically annotated from API 50CH acid
68381Automatically annotated from API rID32STR
68382Automatically annotated from API zym
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
74816Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID7715.1StrainInfo: A central database for resolving microbial strain identifiers
123384Curators of the CIPCollection of Institut Pasteur (CIP 104373)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20104373