Strain identifier

BacDive ID: 5314

Type strain: Yes

Species: Enterococcus gallinarum

Strain Designation: F87/276, PB21

Strain history: CIP <- 1987, NCFB <- 1981, D. Jones

NCBI tax ID(s): 1218091 (strain), 1353 (species)

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General

@ref: 17785

BacDive-ID: 5314

DSM-Number: 24841

keywords: genome sequence, 16S sequence, Bacteria, microaerophile, mesophilic, Gram-positive, coccus-shaped, human pathogen

description: Enterococcus gallinarum F87/276 is a microaerophile, mesophilic, Gram-positive human pathogen that was isolated from chicken intestine.

NCBI tax id

NCBI tax idMatching level
1218091strain
1353species

strain history

@refhistory
17785<- JCM <- ATCC <- NCDO <- D. Jones; F87/276 <- E. M. Barnes; F87/276
67770ATCC 49573 <-- NCDO 2313 <-- D. Jones F87/276.
119515CIP <- 1987, NCFB <- 1981, D. Jones

doi: 10.13145/bacdive5314.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Bacilli
  • order: Lactobacillales
  • family: Enterococcaceae
  • genus: Enterococcus
  • species: Enterococcus gallinarum
  • full scientific name: Enterococcus gallinarum (Bridge and Sneath 1982) Collins et al. 1984

  • synonyms

    • @ref: 20215
    • synonym: Streptococcus gallinarum

@ref: 17785

domain: Bacteria

phylum: Firmicutes

class: Bacilli

order: Lactobacillales

family: Enterococcaceae

genus: Enterococcus

species: Enterococcus gallinarum

full scientific name: Enterococcus gallinarum (Bridge and Sneath 1982) Collins et al. 1984

strain designation: F87/276, PB21

type strain: yes

Morphology

cell morphology

  • @ref: 119515
  • gram stain: positive
  • cell shape: coccus-shaped
  • motility: no

colony morphology

@reftype of hemolysishemolysis abilityincubation period
17785alpha11-2 days
1195151

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
17785COLUMBIA BLOOD MEDIUM (DSMZ Medium 693)yeshttps://mediadive.dsmz.de/medium/693Name: COLUMBIA BLOOD MEDIUM (DSMZ Medium 693) Composition: Defibrinated sheep blood 50.0 g/l Columbia agar base
17785TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92)yeshttps://mediadive.dsmz.de/medium/92Name: TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92) Composition: Trypticase soy broth 30.0 g/l Agar 15.0 g/l Yeast extract 3.0 g/l Distilled water
119515CIP Medium 6yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=6

culture temp

@refgrowthtypetemperaturerange
17785positivegrowth37mesophilic
67770positivegrowth37mesophilic
119515positivegrowth25-41
119515nogrowth10psychrophilic
119515nogrowth45thermophilic

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
17785microaerophile
119515facultative anaerobe

halophily

  • @ref: 119515
  • salt: NaCl
  • growth: positive
  • tested relation: growth
  • concentration: 6.5 %

metabolite utilization

@refmetaboliteutilization activitykind of utilization testedChebi-ID
68371Potassium 5-ketogluconate-builds acid from
68371Potassium 2-ketogluconate-builds acid from
68371gluconate-builds acid from24265
68371L-arabitol-builds acid from18403
68371D-arabitol-builds acid from18333
68371L-fucose-builds acid from18287
68371D-fucose-builds acid from28847
68371D-tagatose-builds acid from16443
68371D-lyxose-builds acid from62318
68371turanose-builds acid from32528
68371gentiobiose-builds acid from28066
68371xylitol-builds acid from17151
68371glycogen-builds acid from28087
68371starch-builds acid from28017
68371raffinose-builds acid from16634
68371melezitose-builds acid from6731
68371inulin-builds acid from15443
68371trehalose-builds acid from27082
68371sucrose-builds acid from17992
68371melibiose-builds acid from28053
68371lactose-builds acid from17716
68371maltose-builds acid from17306
68371cellobiose-builds acid from17057
68371salicin-builds acid from17814
68371esculin-builds acid from4853
68371arbutin-builds acid from18305
68371amygdalin-builds acid from27613
68371N-acetylglucosamine-builds acid from59640
68371methyl alpha-D-glucopyranoside-builds acid from320061
68371methyl alpha-D-mannoside-builds acid from43943
68371D-sorbitol-builds acid from17924
68371D-mannitol-builds acid from16899
68371myo-inositol-builds acid from17268
68371galactitol-builds acid from16813
68371L-rhamnose-builds acid from62345
68371L-sorbose-builds acid from17266
68371D-mannose-builds acid from16024
68371D-fructose-builds acid from15824
68371D-glucose-builds acid from17634
68371D-galactose-builds acid from12936
68371methyl beta-D-xylopyranoside-builds acid from74863
68371ribitol-builds acid from15963
68371L-xylose-builds acid from65328
68371D-xylose-builds acid from65327
68371D-ribose-builds acid from16988
68371L-arabinose-builds acid from30849
68371D-arabinose-builds acid from17108
68371erythritol-builds acid from17113
68371glycerol-builds acid from17754
119515esculin+hydrolysis4853
119515hippurate+hydrolysis606565
119515nitrate-reduction17632
119515nitrite-reduction16301
68381arginine+hydrolysis29016
68381D-ribose+builds acid from16988
68381D-mannitol+builds acid from16899
68381sorbitol-builds acid from30911
68381lactose+builds acid from17716
68381trehalose+builds acid from27082
68381raffinose+builds acid from16634
68381sucrose+builds acid from17992
68381L-arabinose+builds acid from30849
68381D-arabitol-builds acid from18333
68381alpha-cyclodextrin+builds acid from40585
68381hippurate+hydrolysis606565
68381glycogen-builds acid from28087
68381pullulan-builds acid from27941
68381maltose+builds acid from17306
68381melibiose+builds acid from28053
68381melezitose-builds acid from6731
68381methyl beta-D-glucopyranoside+builds acid from320055
68381D-tagatose+builds acid from16443
68381urea-hydrolysis16199

metabolite production

  • @ref: 68381
  • Chebi-ID: 15688
  • metabolite: acetoin
  • production: yes

metabolite tests

@refChebi-IDmetabolitevoges-proskauer-test
6838115688acetoin+
11951515688acetoin-

enzymes

@refvalueactivityec
17785catalase+1.11.1.6
17785cytochrome-c oxidase-1.9.3.1
68382alpha-fucosidase-3.2.1.51
68382alpha-mannosidase-3.2.1.24
68382N-acetyl-beta-glucosaminidase+3.2.1.52
68382beta-glucosidase+3.2.1.21
68382alpha-glucosidase-3.2.1.20
68382beta-glucuronidase+3.2.1.31
68382beta-galactosidase+3.2.1.23
68382alpha-galactosidase+3.2.1.22
68382naphthol-AS-BI-phosphohydrolase+
68382acid phosphatase+3.1.3.2
68382alpha-chymotrypsin+3.4.21.1
68382trypsin-3.4.21.4
68382cystine arylamidase-3.4.11.3
68382valine arylamidase-
68382leucine arylamidase+3.4.11.1
68382lipase (C 14)-
68382esterase lipase (C 8)+
68382esterase (C 4)+
68382alkaline phosphatase-3.1.3.1
68381urease-3.5.1.5
68381beta-mannosidase+3.2.1.25
68381glycyl tryptophan arylamidase+
68381pyrrolidonyl arylamidase+3.4.19.3
68381Alanyl-Phenylalanyl-Proline arylamidase-
68381alpha-galactosidase+3.2.1.22
68381beta-glucuronidase+3.2.1.31
68381beta-glucosidase+3.2.1.21
68381arginine dihydrolase+3.5.3.6
119515oxidase-
119515beta-galactosidase+3.2.1.23
119515alcohol dehydrogenase-1.1.1.1
119515catalase-1.11.1.6
119515gamma-glutamyltransferase-2.3.2.2
119515lysine decarboxylase-4.1.1.18
119515ornithine decarboxylase-4.1.1.17

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
119515--++-+---++++++-++--

API 50CHac

@refGLYERYDARALARARIBDXYLLXYLADOMDXGALGLUFRUMNESBERHADULINOMANSORMDMMDGNAGAMYARBESCSALCELMALLACMELSACTREINUMLZRAFAMDGLYGXLTGENTURLYXTAGDFUCLFUCDARLLARLGNT2KG5KG
119515-------------------------------------------------

API rID32STR

@refADH Argbeta GLUbeta GARbeta GURalpha GALPALRIBMANSORLACTRERAFSACLARADARLCDEXVPAPPAbeta GALPyrAbeta NAGGTAHIPGLYGPULMALMELMLZMbeta DGTAGbeta MANURE
17785++++++/-++-+++++-++-+++++--++-+++-
17785+++++-++-+++++-++--+-++--++-+++-

API biotype100

@refGLUFRUGALTREMNESBEMELSACRAFMTEMALLACLTEMbGaMaGaCELGENMbGuESCRIBARAXYLPLERHAFUCMLZDARLLARLXLTDULTAGGLYINOMANMTLTURSORADOHBGLYXERYMDG3MDGSATMUCLTATDTATMTATDMLTLMLTCATETATETTECITGRTGAT2KG5KGTRYNAGGNTPACPATpOBEQATGTEmOBEBATPPATCMTTGEBETPCEABTHINLATCAPCYTHISSUCFUMGREGYTAVTETNTTNGLNITA3OBUAPTGTTPRODALALALASERMNTPROPTYR2KT
119515+++++--++++-------+-----------+----------------------------+---------------------------------------

Isolation, sampling and environmental information

isolation

@refsample type
17785chicken intestine
46855Chick,intestine of young animal
67770Chicken intestine
119515Animal, Chicken, intestine

isolation source categories

Cat1Cat2Cat3
#Host#Birds#Chicken
#Host Body-Site#Gastrointestinal tract#Large intestine

taxonmaps

  • @ref: 69479
  • File name: preview.99_759.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_13;96_19;97_19;98_605;99_759&stattab=map
  • Last taxonomy: Enterococcus
  • 16S sequence: LC097066
  • Sequence Identity:
  • Total samples: 18861
  • soil counts: 573
  • aquatic counts: 1598
  • animal counts: 15794
  • plant counts: 896

Safety information

risk assessment

@refpathogenicity humanpathogenicity animalbiosafety levelbiosafety level comment
17785yesyes2Risk group (German classification)
1195152Risk group (French classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Enterococcus gallinarum strain ATCC 49573 clone 1 16S-23S ribosomal RNA intergenic spacer, partial sequenceDQ204550222ena1353
20218Enterococcus gallinarum strain ATCC 49573 16S ribosomal RNA gene, partial sequenceKC510236814ena1353
17785Enterococcus gallinarum 16S ribosomal RNA gene, partial sequenceAF0399001506ena1353
67770Enterococcus gallinarum 16S rRNA gene, strain LMG 13129AJ3018331568ena1353
67770Enterococcus gallinarum 16S rRNA gene, strain CECT970TAJ4208051516ena1353
67770Enterococcus gallinarum gene for 16S ribosomal RNA, partial sequence, strain: JCM 8728LC0970661498ena1353

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Enterococcus gallinarum NCTC12359GCA_900447935contigncbi1353
66792Enterococcus gallinarum DSM 24841GCA_001886155scaffoldncbi1353
66792Enterococcus gallinarum NBRC 100675GCA_001544275contigncbi1218091
66792Enterococcus gallinarum NBRC 1006751218091.3wgspatric1218091
66792Enterococcus gallinarum strain DSM 248411353.11wgspatric1353
66792Enterococcus gallinarum strain NCTC123591353.41wgspatric1353
66792Enterococcus gallinarum NBRC 1006752731957567draftimg1218091

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
motileyes51.579no
gram-positiveyes91.394no
anaerobicno95.68yes
aerobicno94.689no
halophileyes67.98no
spore-formingno74.167no
glucose-utilyes86.285no
flagellatedno81.177no
thermophileno99.34yes
glucose-fermentyes86.791no

External links

@ref: 17785

culture collection no.: DSM 24841, ATCC 49573, JCM 8728, CCUG 18658, NCTC 12359, NCDO 2313, BCRC 15477, CCM 4054, CECT 970, CGMCC 1.2517, CGMCC 1.9125, CIP 103013, DSM 20628, LMG 13129, NBRC 100675, NCIMB 702313, NCTC 11428, VTT E-97776, LMG 11207

straininfo link

  • @ref: 74803
  • straininfo: 6584

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny1939593Vancomycin susceptibility and identification of motile enterococci.Vincent S, Knight RG, Green M, Sahm DF, Shlaes DMJ Clin Microbiol10.1128/jcm.29.10.2335-2337.19911991*Bacterial Proteins, Carrier Proteins/metabolism, DNA, Bacterial/genetics/isolation & purification, Drug Resistance, Microbial, Enterococcus/*drug effects/isolation & purification/metabolism, Gram-Positive Bacterial Infections/microbiology, *Hexosyltransferases, Humans, Muramoylpentapeptide Carboxypeptidase/metabolism, Penicillin-Binding Proteins, *Peptidyl Transferases, Sequence Homology, Nucleic Acid, Species Specificity, Vancomycin/*pharmacologyMetabolism
Phylogeny7699030DNA hybridization and contour-clamped homogeneous electric field electrophoresis for identification of enterococci to the species level.Donabedian S, Chow JW, Shlaes DM, Green M, Zervos MJJ Clin Microbiol10.1128/jcm.33.1.141-145.19951995Blotting, Southern, DNA, Bacterial/*genetics, Electrophoresis, Agar Gel/methods, Enterococcus/*classification/genetics, Gram-Negative Bacterial Infections/microbiology, Humans, Nucleic Acid Hybridization, Species SpecificityEnzymology
Pathogenicity16495270Enterococcus gallinarum N04-0414 harbors a VanD-type vancomycin resistance operon and does not contain a D-alanine:D-alanine 2 (ddl2) gene.Boyd DA, Miller MA, Mulvey MRAntimicrob Agents Chemother10.1128/AAC.50.3.1067-1070.20062006Alanine/*genetics, Amino Acid Sequence, Base Sequence, Enterococcus/drug effects/*genetics, *Genes, Bacterial, Microbial Sensitivity Tests, Molecular Sequence Data, Multigene Family/drug effects/genetics, *Operon, Vancomycin/pharmacology, Vancomycin Resistance/*geneticsGenetics
Metabolism16995534Inhibitory effects of Enterococcus strains obtained from a probiotic product on in vitro growth of Salmonella enterica serovar enteritidis strain IFO3313.Theppangna W, Otsuki K, Murase TJ Food Prot10.4315/0362-028x-69.9.22582006*Antibiosis, Bridged-Ring Compounds/metabolism/pharmacology, Colony Count, Microbial, Enterococcus/*physiology, Enterococcus faecium/metabolism/*physiology, Food Microbiology, Food Preservation/*methods, Hydrogen-Ion Concentration, *Probiotics, Salmonella enteritidis/drug effects/*growth & development, Temperature, Time FactorsBiotechnology

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
17785Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 24841)https://www.dsmz.de/collection/catalogue/details/culture/DSM-24841
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
46855Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 18658)https://www.ccug.se/strain?id=18658
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68371Automatically annotated from API 50CH acid
68381Automatically annotated from API rID32STR
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
74803Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID6584.1StrainInfo: A central database for resolving microbial strain identifiers
119515Curators of the CIPCollection of Institut Pasteur (CIP 103013)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20103013