Strain identifier

BacDive ID: 5310

Type strain: No

Species: Enterococcus gallinarum

Strain Designation: F87/268, PB21

Strain history: <- NCTC <- P.H.A. Sneath, PB21 <- E.M. Barnes, F87/268

NCBI tax ID(s): 1353 (species)

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General

@ref: 8976

BacDive-ID: 5310

DSM-Number: 20628

keywords: genome sequence, Bacteria, microaerophile, Gram-positive, human pathogen

description: Enterococcus gallinarum F87/268 is a microaerophile, Gram-positive human pathogen that was isolated from chicken intestine.

NCBI tax id

  • NCBI tax id: 1353
  • Matching level: species

strain history

  • @ref: 8976
  • history: <- NCTC <- P.H.A. Sneath, PB21 <- E.M. Barnes, F87/268

doi: 10.13145/bacdive5310.20240916.9.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Bacilli
  • order: Lactobacillales
  • family: Enterococcaceae
  • genus: Enterococcus
  • species: Enterococcus gallinarum
  • full scientific name: Enterococcus gallinarum (Bridge and Sneath 1982) Collins et al. 1984

  • synonyms

    • @ref: 20215
    • synonym: Streptococcus gallinarum

@ref: 8976

domain: Bacteria

phylum: Firmicutes

class: Bacilli

order: Lactobacillales

family: Enterococcaceae

genus: Enterococcus

species: Enterococcus gallinarum

full scientific name: Enterococcus gallinarum (Bridge and Sneath 1982) Collins et al. 1984

strain designation: F87/268, PB21

type strain: no

Morphology

colony morphology

  • @ref: 8976
  • type of hemolysis: alpha
  • hemolysis ability: 1

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
8976COLUMBIA BLOOD MEDIUM (DSMZ Medium 693)yeshttps://mediadive.dsmz.de/medium/693Name: COLUMBIA BLOOD MEDIUM (DSMZ Medium 693) Composition: Defibrinated sheep blood 50.0 g/l Columbia agar base
8976TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92)yeshttps://mediadive.dsmz.de/medium/92Name: TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92) Composition: Trypticase soy broth 30.0 g/l Agar 15.0 g/l Yeast extract 3.0 g/l Distilled water
35468MEDIUM 6 - Columbia agar with 10 % horse bloodyesDistilled water make up to (1000.000 ml);Columbia agar (39.000 g);Horseblood (100.000 ml)

culture temp

@refgrowthtypetemperature
8976positivegrowth37
35468positivegrowth37

Physiology and metabolism

oxygen tolerance

  • @ref: 8976
  • oxygen tolerance: microaerophile

murein

  • @ref: 8976
  • murein short key: A11.31
  • type: A4alpha L-Lys-D-Asp

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
6837028087glycogen-builds acid from
6837028017starch+builds acid from
6837016634raffinose+builds acid from
6837015443inulin+builds acid from
6837027082trehalose+builds acid from
6837017716lactose+builds acid from
6837017924D-sorbitol-builds acid from
6837016899D-mannitol+builds acid from
6837030849L-arabinose+builds acid from
6837016988D-ribose+builds acid from
6837029016arginine+hydrolysis
683704853esculin+hydrolysis
68370606565hippurate+hydrolysis
6838129016arginine+hydrolysis
6838116988D-ribose+builds acid from
6838116899D-mannitol+builds acid from
6838130911sorbitol-builds acid from
6838117716lactose+builds acid from
6838127082trehalose+builds acid from
6838116634raffinose+builds acid from
6838117992sucrose+builds acid from
6838130849L-arabinose+builds acid from
6838118333D-arabitol-builds acid from
6838140585alpha-cyclodextrin+builds acid from
68381606565hippurate+hydrolysis
6838128087glycogen-builds acid from
6838127941pullulan-builds acid from
6838117306maltose+builds acid from
6838128053melibiose+builds acid from
683816731melezitose-builds acid from
6838116443D-tagatose+builds acid from
6838116199urea-hydrolysis

metabolite production

  • @ref: 68370
  • Chebi-ID: 15688
  • metabolite: acetoin
  • production: yes

metabolite tests

  • @ref: 68370
  • Chebi-ID: 15688
  • metabolite: acetoin
  • voges-proskauer-test: +

enzymes

@refvalueactivityec
8976catalase-1.11.1.6
8976cytochrome-c oxidase-1.9.3.1
68381urease-3.5.1.5
68381glycyl tryptophan arylamidase+
68381pyrrolidonyl arylamidase+3.4.19.3
68381beta-galactosidase+3.2.1.23
68381Alanyl-Phenylalanyl-Proline arylamidase-
68381alkaline phosphatase-3.1.3.1
68381alpha-galactosidase+3.2.1.22
68381beta-glucuronidase+3.2.1.31
68381beta-glucosidase+3.2.1.21
68381arginine dihydrolase+3.5.3.6
68370arginine dihydrolase+3.5.3.6
68370leucine arylamidase+3.4.11.1
68370alkaline phosphatase-3.1.3.1
68370beta-galactosidase+3.2.1.23
68370beta-glucuronidase-3.2.1.31
68370alpha-galactosidase+3.2.1.22
68370pyrrolidonyl arylamidase+3.4.19.3
68370beta-glucosidase+3.2.1.21

API 20STR

@refVPHIPESCPYRAalpha GALbeta GURbeta GALPALLAPADHRIBARAMANSORLACTREINURAFAMDGLYGbeta HEM
8976+++++-+-+++++-+++++--

API rID32STR

@refADH Argbeta GLUbeta GARbeta GURalpha GALPALRIBMANSORLACTRERAFSACLARADARLCDEXVPAPPAbeta GALPyrAbeta NAGGTAHIPGLYGPULMALMELMLZMbeta DGTAGbeta MANURE
8976++-++-++-+++++-++/--+++/-++--++-++--
8976+++++-++-+++++-++-+++++--++--++-

Isolation, sampling and environmental information

isolation

  • @ref: 8976
  • sample type: chicken intestine

isolation source categories

Cat1Cat2Cat3
#Host#Birds#Chicken
#Host Body-Site#Gastrointestinal tract#Large intestine

Safety information

risk assessment

  • @ref: 8976
  • pathogenicity human: yes
  • pathogenicity animal: yes
  • biosafety level: 2
  • biosafety level comment: Risk group (German classification)

Sequence information

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Enterococcus faecalis ATCC 35038GCA_000392955scaffoldncbi1169290
66792Enterococcus faecalis ATCC 350381169290.3wgspatric1169290
66792Enterococcus faecalis ATCC 350382563366777draftimg1169290

GC content

  • @ref: 8976
  • GC-content: 39.8
  • method: thermal denaturation, midpoint method (Tm)

Genome-based predictions

predictions

@reftraitmodeldescriptionpredictionconfidencetraining_data
69481spore-formingspore-formingAbility to form endo- or exosporesyes59no
69480gram-positivegram-positivePositive reaction to Gram-stainingyes88.173no
69480anaerobicanaerobicAbility to grow under anoxygenic conditions (including facultative anaerobes)no88.782yes
69480aerobicaerobicAbility to grow under oxygenic conditions (including facultative aerobes)no92.9no
69480spore-formingspore-formingAbility to form endo- or exosporesno75.606no
69480thermophilicthermophileAbility to grow at temperatures above or equal to 45°Cno94.5yes
69480flagellatedmotile2+Ability to perform flagellated movementno88.5no

External links

@ref: 8976

culture collection no.: DSM 20628, ATCC 35038, NCTC 11428

straininfo link

  • @ref: 74800
  • straininfo: 343905

literature

  • topic: Metabolism
  • Pubmed-ID: 8627044
  • title: Augmented production of extracellular superoxide by blood isolates of Enterococcus faecalis.
  • authors: Huycke MM, Joyce W, Wack MF
  • journal: J Infect Dis
  • DOI: 10.1093/infdis/173.3.743
  • year: 1996
  • mesh: Bacteremia/*microbiology, Endocarditis, Bacterial/microbiology, Enterococcus faecalis/isolation & purification/*metabolism/pathogenicity, Extracellular Space/metabolism, Feces/microbiology, Gram-Positive Bacterial Infections/*microbiology, Humans, In Vitro Techniques, Lactococcus lactis/metabolism, Pseudomonas Infections/microbiology, Pseudomonas aeruginosa/isolation & purification/metabolism, Superoxides/*metabolism, Virulence
  • topic2: Enzymology

Reference

@idauthorscataloguedoi/urltitle
8976Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 20628)https://www.dsmz.de/collection/catalogue/details/culture/DSM-20628
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
35468Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/14763
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
68370Automatically annotated from API 20STR
68381Automatically annotated from API rID32STR
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch10.21203/rs.3.rs-2527258/v1Predictions based on the model GenomeNet Sporulation v. 1
74800Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID343905.1StrainInfo: A central database for resolving microbial strain identifiers