Strain identifier

BacDive ID: 5299

Type strain: No

Species: Enterococcus faecium

Strain Designation: 70/90, vanA

Strain history: CIP <- 2009, DSMZ <- G. Rueter: strain 70/90 <- W. Witte and I. Klare, RKI <- J. Wagner, Freie Univ. Berlin, Germany: strain vanA

NCBI tax ID(s): 1352 (species)

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General

@ref: 5098

BacDive-ID: 5299

DSM-Number: 13590

keywords: Bacteria, microaerophile, mesophilic, Gram-positive, coccus-shaped, antibiotic resistance

description: Enterococcus faecium 70/90 is a microaerophile, mesophilic, Gram-positive bacterium that has multiple antibiotic resistances and was isolated from human faeces, rectal swab.

NCBI tax id

  • NCBI tax id: 1352
  • Matching level: species

strain history

@refhistory
5098<- G. Reuter; 70/90 <- W. Witte and I. Klare, RKI <- J. Wagner, Freie Univ. Berlin
119226CIP <- 2009, DSMZ <- G. Rueter: strain 70/90 <- W. Witte and I. Klare, RKI <- J. Wagner, Freie Univ. Berlin, Germany: strain vanA

doi: 10.13145/bacdive5299.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Bacilli
  • order: Lactobacillales
  • family: Enterococcaceae
  • genus: Enterococcus
  • species: Enterococcus faecium
  • full scientific name: Enterococcus faecium (Orla-Jensen 1919) Schleifer and Kilpper-Bälz 1984

  • synonyms

    • @ref: 20215
    • synonym: Streptococcus faecium

@ref: 5098

domain: Bacteria

phylum: Firmicutes

class: Bacilli

order: Lactobacillales

family: Enterococcaceae

genus: Enterococcus

species: Enterococcus faecium

full scientific name: Enterococcus faecium (Orla-Jensen 1919) Schleifer and Kilpper-Bälz 1984

strain designation: 70/90, vanA

type strain: no

Morphology

cell morphology

  • @ref: 119226
  • gram stain: positive
  • cell shape: coccus-shaped
  • motility: no

colony morphology

  • @ref: 5098
  • type of hemolysis: alpha
  • hemolysis ability: 1
  • incubation period: 1-2 days

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
5098COLUMBIA BLOOD MEDIUM (DSMZ Medium 693)yeshttps://mediadive.dsmz.de/medium/693Name: COLUMBIA BLOOD MEDIUM (DSMZ Medium 693) Composition: Defibrinated sheep blood 50.0 g/l Columbia agar base
5098TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92)yeshttps://mediadive.dsmz.de/medium/92Name: TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92) Composition: Trypticase soy broth 30.0 g/l Agar 15.0 g/l Yeast extract 3.0 g/l Distilled water
36873MEDIUM 29- Brain heart agaryesDistilled water make up to (1000.000 ml);Brain heart infusion agar (52.000 g)
119226CIP Medium 29yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=29

culture temp

@refgrowthtypetemperaturerange
5098positivegrowth37mesophilic
36873positivegrowth37mesophilic

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
5098microaerophile
119226facultative anaerobe

antibiogram

@refmediumincubation temperatureincubation timeoxygen conditionPenicillin Goxacillinampicillinticarcillinmezlocillincefalotincefazolincefotaximeaztreonamimipenemtetracyclinechloramphenicolgentamycinamikacinvancomycinerythromycinlincomycinofloxacinnorfloxacincolistinpipemidic acidnitrofurantoinbacitracinpolymyxin bkanamycinneomycindoxycyclineceftriaxoneclindamycinfosfomycinmoxifloxacinlinezolidnystatinquinupristin/dalfopristinteicoplaninpiperacillin/tazobactam
5098Mueller-Hinton Agar371microaerophil00120100000610241212-1400018200012001012-1418002026300186-86-8
5098Mueller-Hinton Agar37microaerophil0012010000010022121400018200010001214160020242801806
5098Mueller-Hinton Agar37microaerophil0012010000010022121400018200010001214160020242801806

observation

  • @ref: 5098
  • observation: Vancomycin resistant

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
11922617632nitrate-reduction
11922616301nitrite-reduction
6838129016arginine+hydrolysis
6838116988D-ribose+builds acid from
6838116899D-mannitol+builds acid from
6838130911sorbitol-builds acid from
6838117716lactose+builds acid from
6838127082trehalose+builds acid from
6838116634raffinose-builds acid from
6838117992sucrose-builds acid from
6838130849L-arabinose+builds acid from
6838118333D-arabitol-builds acid from
6838140585alpha-cyclodextrin+builds acid from
6838128087glycogen-builds acid from
6838127941pullulan-builds acid from
6838117306maltose+builds acid from
683816731melezitose-builds acid from
68381320055methyl beta-D-glucopyranoside+builds acid from
6838116443D-tagatose-builds acid from
6838116199urea-hydrolysis

metabolite production

@refChebi-IDmetaboliteproduction
11922635581indoleno
6838115688acetoinyes

metabolite tests

  • @ref: 68381
  • Chebi-ID: 15688
  • metabolite: acetoin
  • voges-proskauer-test: +

enzymes

@refvalueactivityec
5098catalase-1.11.1.6
5098cytochrome-c oxidase-1.9.3.1
68382alpha-fucosidase-3.2.1.51
68382alpha-mannosidase-3.2.1.24
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382beta-glucosidase-3.2.1.21
68382alpha-glucosidase-3.2.1.20
68382beta-glucuronidase+3.2.1.31
68382beta-galactosidase-3.2.1.23
68382alpha-galactosidase-3.2.1.22
68382naphthol-AS-BI-phosphohydrolase+
68382acid phosphatase+3.1.3.2
68382alpha-chymotrypsin+3.4.21.1
68382trypsin-3.4.21.4
68382cystine arylamidase-3.4.11.3
68382valine arylamidase-
68382leucine arylamidase+3.4.11.1
68382lipase (C 14)-
68382esterase lipase (C 8)+
68382esterase (C 4)-
68382alkaline phosphatase-3.1.3.1
68381urease-3.5.1.5
68381glycyl tryptophan arylamidase+
68381N-acetyl-beta-glucosaminidase+3.2.1.52
68381pyrrolidonyl arylamidase+3.4.19.3
68381beta-galactosidase+3.2.1.23
68381Alanyl-Phenylalanyl-Proline arylamidase-
68381alkaline phosphatase-3.1.3.1
68381beta-glucuronidase-3.2.1.31
68381beta-glucosidase+3.2.1.21
68381arginine dihydrolase+3.5.3.6
119226oxidase-
119226catalase-1.11.1.6
119226urease-3.5.1.5

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
119226---+-+---+++--+-----

API rID32STR

@refADH Argbeta GLUbeta GARbeta GURalpha GALPALRIBMANSORLACTRERAFSACLARADARLCDEXVPAPPAbeta GALPyrAbeta NAGGTAHIPGLYGPULMALMELMLZMbeta DGTAGbeta MANURE
5098++----++-++--+-++-+++++--+--+---
5098+++---++-++--+-++-+++++--+--+-+-
5098++--+/--++-++--+-++-++++---++-+---
5098+++---++-++--+-++-++++---+--+-+-
5098+++---++-++--+-++-+++++/---+--+-+-

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinentlatitudelongitude
5098human faeces, rectal swabBerlinGermanyDEUEurope52.518613.4083
119226Human, Faeces, rectal swab

isolation source categories

Cat1Cat2Cat3
#Infection#Patient#Swab
#Host#Human
#Host Body-Site#Gastrointestinal tract#Rectum
#Host Body Product#Gastrointestinal tract#Feces (Stool)

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
50982Risk group (German classification)
1192262Risk group (French classification)

External links

@ref: 5098

culture collection no.: DSM 13590, CIP 110055, WDCM 00154, CECT 8224, NCTC 13633

straininfo link

  • @ref: 74789
  • straininfo: 143343

literature

topicPubmed-IDtitleauthorsjournalDOIyearmesh
Pathogenicity7798650Comparison of different beta-lactam-glycopeptide-gentamicin combinations for an experimental endocarditis caused by a highly beta-lactam-resistant and highly glycopeptide-resistant isolate of Enterococcus faecium.Caron F, Pestel M, Kitzis MD, Lemeland JF, Humbert G, Gutmann LJ Infect Dis10.1093/infdis/171.1.1061995Animals, Ceftriaxone/pharmacology/therapeutic use, Drug Resistance, Microbial, Drug Synergism, Drug Therapy, Combination/pharmacology/*therapeutic use, Endocarditis, Bacterial/*drug therapy/microbiology, Enterococcus faecium/*drug effects, Female, Gentamicins/pharmacology/therapeutic use, Gram-Positive Bacterial Infections/*drug therapy/microbiology, Penicillins/pharmacology/therapeutic use, Rabbits, Random Allocation, Recurrence, Teicoplanin/pharmacology/therapeutic use, Vancomycin/pharmacology/therapeutic use
23381026Effect of Ottoman Viper (Montivipera xanthina (Gray, 1849)) Venom on Various Cancer Cells and on Microorganisms.Yalcin HT, Ozen MO, Gocmen B, Nalbantsoy ACytotechnology10.1007/s10616-013-9540-z2013
Pathogenicity34641589Sintered and 3D-Printed Bulks of MgB2-Based Materials with Antimicrobial Properties.Badica P, Batalu ND, Chifiriuc MC, Burdusel M, Grigoroscuta MA, Aldica GV, Pasuk I, Kuncser A, Popa M, Agostino A, Operti L, Padhi SK, Bonino V, Truccato MMolecules10.3390/molecules261960452021Anti-Infective Agents/*pharmacology, Bacteria/*drug effects, Boron Compounds/*pharmacology, Candida parapsilosis/drug effects, Enterococcus faecalis/drug effects, Enterococcus faecium/drug effects, Escherichia coli/drug effects, Fungi/*drug effects, Magnesium Compounds/*pharmacology, Microbial Sensitivity Tests, Polyesters/pharmacology, Printing, Three-Dimensional, Pseudomonas aeruginosa/drug effects, Staphylococcus aureus/drug effects

Reference

@idauthorscataloguedoi/urltitle
5098Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 13590)https://www.dsmz.de/collection/catalogue/details/culture/DSM-13590
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
36873Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/7870
68381Automatically annotated from API rID32STR
68382Automatically annotated from API zym
74789Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID143343.1StrainInfo: A central database for resolving microbial strain identifiers
119226Curators of the CIPCollection of Institut Pasteur (CIP 110055)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20110055