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Strain identifier

BacDive ID: 5276

Type strain: Yes

Species: Enterococcus casseliflavus

Strain Designation: 20

Strain history: ATCC 25788 <-- J. O. Mundt 20.

NCBI tax ID(s): 1218087 (strain), 37734 (species)

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For citation purpose refer to the digital object identifier (doi) of the current version.
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General

@ref: 9002

BacDive-ID: 5276

DSM-Number: 20680

keywords: genome sequence, 16S sequence, Bacteria, microaerophile, mesophilic

description: Enterococcus casseliflavus 20 is a microaerophile, mesophilic bacterium that was isolated from plant material.

NCBI tax id

NCBI tax idMatching level
37734species
1218087strain

strain history

doi: 10.13145/bacdive5276.20221219.7.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Bacilli
  • order: Lactobacillales
  • family: Enterococcaceae
  • genus: Enterococcus
  • species: Enterococcus casseliflavus
  • full scientific name: Enterococcus casseliflavus (ex Vaughan et al. 1979) Collins et al. 1984

  • synonyms

    @refsynonym
    20215Streptococcus casseliflavus
    20215Streptococcus faecium subsp. casseliflavus
    20215Enterococcus flavescens

@ref: 9002

domain: Bacteria

phylum: Firmicutes

class: Bacilli

order: Lactobacillales

family: Enterococcaceae

genus: Enterococcus

species: Enterococcus casseliflavus

full scientific name: Enterococcus casseliflavus (ex Vaughn et al. 1979) Collins et al. 1984

strain designation: 20

type strain: yes

Morphology

cell morphology

  • @ref: 69480
  • gram stain: positive
  • confidence: 93.269

colony morphology

  • @ref: 9002
  • type of hemolysis: alpha
  • incubation period: 1-2 days

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
9002COLUMBIA BLOOD MEDIUM (DSMZ Medium 693)yeshttps://mediadive.dsmz.de/medium/693Name: COLUMBIA BLOOD MEDIUM (DSMZ Medium 693) Composition: Defibrinated sheep blood 50.0 g/l Columbia agar base
9002TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92)yeshttps://mediadive.dsmz.de/medium/92Name: TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92) Composition: Trypticase soy broth 30.0 g/l Agar 15.0 g/l Yeast extract 3.0 g/l Distilled water
38111MEDIUM 29- Brain heart agaryesDistilled water make up to (1000.000 ml);Brain heart infusion agar (52.000 g)

culture temp

@refgrowthtypetemperaturerange
9002positivegrowth37mesophilic
38111positivegrowth37mesophilic
46854positivegrowth30mesophilic
67770positivegrowth37mesophilic

Physiology and metabolism

oxygen tolerance

  • @ref: 9002
  • oxygen tolerance: microaerophile

spore formation

  • @ref: 69480
  • spore formation: no
  • confidence: 97.596

halophily

  • @ref: 69480
  • halophily level: non-halophilic
  • confidence: 83.243

murein

  • @ref: 9002
  • murein short key: A11.31
  • type: A4alpha L-Lys-D-Asp

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
6838116199urea-hydrolysis
6838116443D-tagatose-builds acid from
68381320055methyl beta-D-glucopyranoside+builds acid from
6838128053melibiose+builds acid from
6838117306maltose+builds acid from
6838127941pullulan-builds acid from
6838128087glycogen-builds acid from
68381606565hippurate-hydrolysis
6838140585alpha-cyclodextrin-builds acid from
6838118333D-arabitol-builds acid from
6838130849L-arabinose+builds acid from
6838117992sucrose+builds acid from
6838116634raffinose+builds acid from
6838127082trehalose+builds acid from
6838117716lactose+builds acid from
6838130911sorbitol-builds acid from
6838116899D-mannitol+builds acid from
6838116988D-ribose+builds acid from
6837928087glycogen-fermentation
6837917992sucrose+fermentation
6837917716lactose+fermentation
6837917306maltose+fermentation
6837916899D-mannitol+fermentation
6837965327D-xylose+fermentation
6837916988D-ribose+fermentation
683795291gelatin-hydrolysis
6837916199urea-hydrolysis
683794853esculin+hydrolysis
6837917632nitrate-reduction

metabolite production

  • @ref: 68381
  • Chebi-ID: 15688
  • metabolite: acetoin
  • production: yes

metabolite tests

  • @ref: 68381
  • Chebi-ID: 15688
  • metabolite: acetoin
  • voges-proskauer-test: +

enzymes

@refvalueactivityec
9002catalase-1.11.1.6
9002cytochrome-c oxidase-1.9.3.1
68381urease-3.5.1.5
68381beta-mannosidase+3.2.1.25
68381glycyl tryptophan arylamidase+
68381pyrrolidonyl arylamidase+3.4.19.3
68381beta-galactosidase+3.2.1.23
68381Alanyl-Phenylalanyl-Proline arylamidase-
68381alkaline phosphatase-3.1.3.1
68381alpha-galactosidase+3.2.1.22
68381beta-glucuronidase-3.2.1.31
68381beta-glucosidase+3.2.1.21
68379catalase-1.11.1.6
68379gelatinase-
68379urease-3.5.1.5
68379beta-glucosidase+3.2.1.21
68379N-acetyl-beta-glucosaminidase+3.2.1.52
68379alpha-glucosidase+3.2.1.20
68379beta-galactosidase+3.2.1.23
68379beta-glucuronidase+3.2.1.31
68379alkaline phosphatase-3.1.3.1
68379pyrrolidonyl arylamidase+3.4.19.3
68379pyrazinamidase-3.5.1.B15

API coryne

@refNITPYZPYRAPALbeta GURbeta GALalpha GLUbeta NAGESCUREGELControlRIBXYLMANMALLACSACGLYGCAT
46854--+-+++++---++++++--

API rID32STR

@refADH Argbeta GLUbeta GARbeta GURalpha GALPALRIBMANSORLACTRERAFSACLARADARLCDEXVPAPPAbeta GALPyrAbeta NAGGTAHIPGLYGPULMALMELMLZMbeta DGTAGbeta MANURE
9002-++-+-++-+++++--+-++-+---++-+-+-
9002-++/--+-++-+++++--+-++-+---++-+-+-
9002++--+-++-+++++--+-++++---+++/-+-+-

Isolation, sampling and environmental information

isolation

@refsample typesampling date
9002plant material
46854Plant material1984
67770Plant material

isolation source categories

  • Cat1: #Host
  • Cat2: #Plants

taxonmaps

  • @ref: 69479
  • File name: preview.99_759.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_13;96_19;97_19;98_605;99_759&stattab=map
  • Last taxonomy: Enterococcus
  • 16S sequence: Y18161
  • Sequence Identity:
  • Total samples: 18861
  • soil counts: 573
  • aquatic counts: 1598
  • animal counts: 15794
  • plant counts: 896

Safety information

risk assessment

  • @ref: 9002
  • biosafety level: 2
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Enterococcus casseliflavus 16S ribosomal RNA gene, partial sequenceAF0399031509ena37734
20218Enterococcus casseliflavus strain ATCC 25788 16S ribosomal RNA gene, partial sequenceKC510229814ena37734
20218E.casseliflavus 16S rRNA gene, partial (strain ATTC 25788 (CCUG 18657))Y12908366ena37734
20218Enterococcus casseliflavus strain CCUG 18657 clone 1 16S-23S ribosomal RNA intergenic spacer, partial sequenceDQ204549226ena37734
20218Enterococcus casseliflavus 16S rRNA gene, strain CECT969TAJ4208041451ena37734
20218Enterococcus casseliflavus 16S rRNA gene, strain LMG 10745AJ3018261904ena37734
20218Enterococcus casseliflavus 16S rRNA gene, strain NCIMB 11449Y181611421ena37734
9002Enterococcus casseliflavus gene for 16S rRNA, partial sequence, strain: NBRC 100478AB6811761425ena37734

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Enterococcus casseliflavus NCTC12361GCA_900447665contigncbi37734
66792Enterococcus casseliflavus DSM 20680GCA_001885845scaffoldncbi37734
66792Enterococcus casseliflavus NBRC 100478 NBRC 100478GCA_001544095contigncbi1218087
66792Enterococcus casseliflavus NBRC 1004781218087.3wgspatric1218087
66792Enterococcus casseliflavus strain DSM 2068037734.14wgspatric37734
66792Enterococcus casseliflavus strain FDAARGOS_1120 strain Not applicable37734.151completepatric37734
66792Enterococcus casseliflavus strain NCTC1236137734.39wgspatric37734
66792Enterococcus casseliflavus NBRC 1004782731957559draftimg1218087
66792Acinetobacter ursingii UBA1533GCA_002323285scaffoldpatric108980
66792[Eubacterium] rectale D31t1_170403_H3GCA_015559035scaffoldpatric39491
66792Vibrio fluvialis PV3GCA_022290255scaffoldpatric676
66792Streptococcus oralis O1-3GCA_023110555scaffoldpatric1303

GC content

  • @ref: 9002
  • GC-content: 43.4
  • method: thermal denaturation, midpoint method (Tm)

External links

@ref: 9002

culture collection no.: DSM 20680, ATCC 25788, CCM 2478, NCDO 2372, NCIB 11449, CCUG 18657, NCTC 12361, JCM 8723, CECT 969, CGMCC 1.9124, CIP 103018, KCTC 3638, LMG 10745, NBRC 100478, NCIMB 11449, NRRL B-3502, VTT E-97775

straininfo link

@refpassport
20218http://www.straininfo.net/strains/9151
20218http://www.straininfo.net/strains/9150
20218http://www.straininfo.net/strains/150563
20218http://www.straininfo.net/strains/104578
20218http://www.straininfo.net/strains/133662

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny1939593Vancomycin susceptibility and identification of motile enterococci.Vincent S, Knight RG, Green M, Sahm DF, Shlaes DMJ Clin Microbiol10.1128/jcm.29.10.2335-2337.19911991*Bacterial Proteins, Carrier Proteins/metabolism, DNA, Bacterial/genetics/isolation & purification, Drug Resistance, Microbial, Enterococcus/*drug effects/isolation & purification/metabolism, Gram-Positive Bacterial Infections/microbiology, *Hexosyltransferases, Humans, Muramoylpentapeptide Carboxypeptidase/metabolism, Penicillin-Binding Proteins, *Peptidyl Transferases, Sequence Homology, Nucleic Acid, Species Specificity, Vancomycin/*pharmacologyMetabolism
Phylogeny7699030DNA hybridization and contour-clamped homogeneous electric field electrophoresis for identification of enterococci to the species level.Donabedian S, Chow JW, Shlaes DM, Green M, Zervos MJJ Clin Microbiol10.1128/jcm.33.1.141-145.19951995Blotting, Southern, DNA, Bacterial/*genetics, Electrophoresis, Agar Gel/methods, Enterococcus/*classification/genetics, Gram-Negative Bacterial Infections/microbiology, Humans, Nucleic Acid Hybridization, Species SpecificityEnzymology
Phylogeny7960141Resistance of Enterococcus faecium to neutrophil-mediated phagocytosis.Arduino RC, Jacques-Palaz K, Murray BE, Rakita RMInfect Immun10.1128/iai.62.12.5587-5594.19941994Bacterial Capsules/*chemistry, Enterococcus faecium/classification/*immunology/pathogenicity/ultrastructure, Humans, Microscopy, Electron, Microscopy, Fluorescence, N-Acetylneuraminic Acid, Neutrophils/*immunology/ultrastructure, Opsonin Proteins, *Phagocytosis, Sialic Acids/analysis, Species Specificity, VirulencePathogenicity
Enzymology7986009Analysis of genes encoding D-alanine-D-alanine ligase-related enzymes in Enterococcus casseliflavus and Enterococcus flavescens.Navarro F, Courvalin PAntimicrob Agents Chemother10.1128/AAC.38.8.17881994Amino Acid Sequence, Bacterial Proteins/genetics, Base Sequence, Drug Resistance, Microbial, Enterococcus/*genetics, *Genes, Bacterial, Molecular Sequence Data, Peptide Synthases/*genetics, Vancomycin/pharmacologyGenetics
Enzymology9294159Bacterial resistance to vancomycin: overproduction, purification, and characterization of VanC2 from Enterococcus casseliflavus as a D-Ala-D-Ser ligase.Park IS, Lin CH, Walsh CTProc Natl Acad Sci U S A10.1073/pnas.94.19.100401997*Bacterial Proteins, Cloning, Molecular, Drug Resistance, Microbial/genetics, Enterococcus/*enzymology, Escherichia coli/genetics, Peptide Synthases/*genetics/isolation & purification/metabolism, Vancomycin/*pharmacologyBiotechnology
Metabolism12234834Biochemical and genetic characterization of the vanC-2 vancomycin resistance gene cluster of Enterococcus casseliflavus ATCC 25788.Dutta I, Reynolds PEAntimicrob Agents Chemother10.1128/AAC.46.10.3125-3132.20022002*Bacterial Proteins, Cloning, Molecular, Enterococcus/*drug effects/genetics/growth & development, *Gene Expression Regulation, Bacterial, Microbial Sensitivity Tests, Molecular Sequence Data, *Multigene Family, Peptide Synthases/*genetics/*metabolism, Peptidoglycan/metabolism, Plasmids/genetics, Sequence Analysis, DNA, Serine/metabolism, Vancomycin Resistance/*geneticsEnzymology
Enzymology12403118Nucleotide substitutions in vanC-2 gene of Enterococcus casseliflavus isolates obtained from chickens.Murase T, Mito Y, Otsuki K, Suzuki R, Yamai SEpidemiol Infect10.1017/s09502688020075012002Animals, *Bacterial Proteins, Base Sequence, Brazil/epidemiology, Chickens/*microbiology, China/epidemiology, DNA, Bacterial/*genetics, Electrophoresis, Gel, Pulsed-Field, Enterococcus/*genetics/isolation & purification, Food Contamination, *Food Microbiology, Gram-Positive Bacterial Infections/*prevention & control, Humans, Japan/epidemiology, Molecular Sequence Data, Peptide Synthases/*geneticsBiotechnology
Enzymology12615874The vanC-3 vancomycin resistance gene cluster of Enterococcus flavescens CCM 439.Dutta I, Reynolds PEJ Antimicrob Chemother10.1093/jac/dkg1312003Bacterial Proteins/*genetics/isolation & purification, Enterococcus/*genetics/isolation & purification, Humans, Molecular Sequence Data, Multigene Family/*genetics, Phenotype, Polymerase Chain Reaction, Polymorphism, Restriction Fragment Length, Vancomycin Resistance/*geneticsPhylogeny
Phylogeny34919037Enterococcus innesii sp. nov., isolated from the wax moth Galleria mellonella.Gooch HCC, Kiu R, Rudder S, Baker DJ, Hall LJ, Maxwell AInt J Syst Evol Microbiol10.1099/ijsem.0.0051682021Animals, Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Enterococcus/*classification/isolation & purification, Fatty Acids/chemistry, *Moths/microbiology, Nucleic Acid Hybridization, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNATranscriptome
Phylogeny35456876Enterococcus casseliflavus KB1733 Isolated from a Traditional Japanese Pickle Induces Interferon-Lambda Production in Human Intestinal Epithelial Cells.Satomi S, Kokubu D, Inoue T, Sugiyama M, Mizokami M, Suzuki S, Murata KMicroorganisms10.3390/microorganisms100408272022Biotechnology

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
9002Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 20680)https://www.dsmz.de/collection/catalogue/details/culture/DSM-20680
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
38111Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/14769
46854Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 18657)https://www.ccug.se/strain?id=18657
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68379Automatically annotated from API Coryne
68381Automatically annotated from API rID32STR
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)