Strain identifier

BacDive ID: 5276

Type strain: Yes

Species: Enterococcus casseliflavus

Strain Designation: 20, W 99

Strain history: CIP <- 1987, NCDO <- 1981, D. Jones, UK: strain W 99 <- J.O. Mundt: strain 20, Streptococcus faecium subsp. casseliflavus

NCBI tax ID(s): 1218087 (strain), 37734 (species)

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General

@ref: 9002

BacDive-ID: 5276

DSM-Number: 20680

keywords: genome sequence, 16S sequence, Bacteria, microaerophile, mesophilic, Gram-positive

description: Enterococcus casseliflavus 20 is a microaerophile, mesophilic, Gram-positive bacterium that was isolated from plant material.

NCBI tax id

NCBI tax idMatching level
1218087strain
37734species

strain history

@refhistory
9002<- NCDO <- D. Jones <- (J.O. Mundt, 20)
67770ATCC 25788 <-- J. O. Mundt 20.
117205CIP <- 1987, NCDO <- 1981, D. Jones, UK: strain W 99 <- J.O. Mundt: strain 20, Streptococcus faecium subsp. casseliflavus

doi: 10.13145/bacdive5276.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Bacilli
  • order: Lactobacillales
  • family: Enterococcaceae
  • genus: Enterococcus
  • species: Enterococcus casseliflavus
  • full scientific name: Enterococcus casseliflavus (ex Vaughan et al. 1979) Collins et al. 1984

  • synonyms

    @refsynonym
    20215Streptococcus faecium subsp. casseliflavus
    20215Enterococcus flavescens
    20215Streptococcus casseliflavus

@ref: 9002

domain: Bacteria

phylum: Firmicutes

class: Bacilli

order: Lactobacillales

family: Enterococcaceae

genus: Enterococcus

species: Enterococcus casseliflavus

full scientific name: Enterococcus casseliflavus (ex Vaughn et al. 1979) Collins et al. 1984

strain designation: 20, W 99

type strain: yes

Morphology

cell morphology

@refgram stainconfidencecell shapemotility
69480positive100
117205positivecoccus-shapedno

colony morphology

@reftype of hemolysishemolysis abilityincubation period
9002alpha11-2 days
1172051

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
9002COLUMBIA BLOOD MEDIUM (DSMZ Medium 693)yeshttps://mediadive.dsmz.de/medium/693Name: COLUMBIA BLOOD MEDIUM (DSMZ Medium 693) Composition: Defibrinated sheep blood 50.0 g/l Columbia agar base
9002TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92)yeshttps://mediadive.dsmz.de/medium/92Name: TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92) Composition: Trypticase soy broth 30.0 g/l Agar 15.0 g/l Yeast extract 3.0 g/l Distilled water
38111MEDIUM 29- Brain heart agaryesDistilled water make up to (1000.000 ml);Brain heart infusion agar (52.000 g)
117205CIP Medium 29yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=29

culture temp

@refgrowthtypetemperaturerange
9002positivegrowth37mesophilic
38111positivegrowth37mesophilic
46854positivegrowth30mesophilic
67770positivegrowth37mesophilic
117205positivegrowth10-45

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
9002microaerophile
117205facultative anaerobe

spore formation

  • @ref: 69480
  • spore formation: no
  • confidence: 99.965

halophily

  • @ref: 117205
  • salt: NaCl
  • growth: positive
  • tested relation: growth
  • concentration: 6.5 %

murein

  • @ref: 9002
  • murein short key: A11.31
  • type: A4alpha L-Lys-D-Asp

metabolite utilization

@refmetaboliteutilization activitykind of utilization testedChebi-ID
68371Potassium 2-ketogluconate-builds acid from
68371gluconate+builds acid from24265
68371L-arabitol-builds acid from18403
68371L-fucose-builds acid from18287
68371D-fucose-builds acid from28847
68371D-tagatose-builds acid from16443
68371D-lyxose-builds acid from62318
68371turanose+builds acid from32528
68371gentiobiose+builds acid from28066
68371xylitol-builds acid from17151
68371glycogen-builds acid from28087
68371starch+builds acid from28017
68371raffinose+builds acid from16634
68371melezitose-builds acid from6731
68371inulin+builds acid from15443
68371trehalose+builds acid from27082
68371sucrose+builds acid from17992
68371melibiose+builds acid from28053
68371lactose+builds acid from17716
68371maltose+builds acid from17306
68371cellobiose+builds acid from17057
68371salicin+builds acid from17814
68371esculin+builds acid from4853
68371arbutin+builds acid from18305
68371amygdalin+builds acid from27613
68371N-acetylglucosamine+builds acid from59640
68371methyl alpha-D-glucopyranoside+builds acid from320061
68371methyl alpha-D-mannoside+builds acid from43943
68371D-sorbitol+builds acid from17924
68371D-mannitol+builds acid from16899
68371myo-inositol-builds acid from17268
68371galactitol-builds acid from16813
68371L-rhamnose+builds acid from62345
68371L-sorbose-builds acid from17266
68371D-mannose+builds acid from16024
68371D-fructose+builds acid from15824
68371D-glucose+builds acid from17634
68371D-galactose+builds acid from12936
68371methyl beta-D-xylopyranoside-builds acid from74863
68371ribitol-builds acid from15963
68371L-xylose-builds acid from65328
68371D-xylose+builds acid from65327
68371D-ribose+builds acid from16988
68371L-arabinose+builds acid from30849
68371D-arabinose-builds acid from17108
68371erythritol-builds acid from17113
68371glycerol+builds acid from17754
117205esculin+hydrolysis4853
117205hippurate-hydrolysis606565
117205nitrate-reduction17632
117205nitrite-reduction16301
68379nitrate-reduction17632
68379esculin+hydrolysis4853
68379urea-hydrolysis16199
68379gelatin-hydrolysis5291
68379D-ribose+fermentation16988
68379D-xylose+fermentation65327
68379D-mannitol+fermentation16899
68379maltose+fermentation17306
68379lactose+fermentation17716
68379sucrose+fermentation17992
68379glycogen-fermentation28087
68381D-ribose+builds acid from16988
68381D-mannitol+builds acid from16899
68381sorbitol-builds acid from30911
68381lactose+builds acid from17716
68381trehalose+builds acid from27082
68381raffinose+builds acid from16634
68381sucrose+builds acid from17992
68381L-arabinose+builds acid from30849
68381D-arabitol-builds acid from18333
68381alpha-cyclodextrin-builds acid from40585
68381hippurate-hydrolysis606565
68381glycogen-builds acid from28087
68381pullulan-builds acid from27941
68381maltose+builds acid from17306
68381melibiose+builds acid from28053
68381methyl beta-D-glucopyranoside+builds acid from320055
68381D-tagatose-builds acid from16443
68381urea-hydrolysis16199

metabolite production

  • @ref: 68381
  • Chebi-ID: 15688
  • metabolite: acetoin
  • production: yes

metabolite tests

@refChebi-IDmetabolitevoges-proskauer-test
6838115688acetoin+
11720515688acetoin+

enzymes

@refvalueactivityec
9002catalase-1.11.1.6
9002cytochrome-c oxidase-1.9.3.1
68382alpha-fucosidase-3.2.1.51
68382alpha-mannosidase-3.2.1.24
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382beta-glucosidase+3.2.1.21
68382alpha-glucosidase-3.2.1.20
68382beta-glucuronidase+3.2.1.31
68382beta-galactosidase+3.2.1.23
68382alpha-galactosidase+3.2.1.22
68382naphthol-AS-BI-phosphohydrolase+
68382acid phosphatase+3.1.3.2
68382alpha-chymotrypsin+3.4.21.1
68382trypsin-3.4.21.4
68382cystine arylamidase-3.4.11.3
68382valine arylamidase-
68382leucine arylamidase+3.4.11.1
68382lipase (C 14)-
68382esterase lipase (C 8)+
68382esterase (C 4)+
68382alkaline phosphatase-3.1.3.1
68381urease-3.5.1.5
68381beta-mannosidase+3.2.1.25
68381glycyl tryptophan arylamidase+
68381pyrrolidonyl arylamidase+3.4.19.3
68381beta-galactosidase+3.2.1.23
68381Alanyl-Phenylalanyl-Proline arylamidase-
68381alkaline phosphatase-3.1.3.1
68381alpha-galactosidase+3.2.1.22
68381beta-glucuronidase-3.2.1.31
68381beta-glucosidase+3.2.1.21
68379catalase-1.11.1.6
68379gelatinase-
68379urease-3.5.1.5
68379beta-glucosidase+3.2.1.21
68379N-acetyl-beta-glucosaminidase+3.2.1.52
68379alpha-glucosidase+3.2.1.20
68379beta-galactosidase+3.2.1.23
68379beta-glucuronidase+3.2.1.31
68379alkaline phosphatase-3.1.3.1
68379pyrrolidonyl arylamidase+3.4.19.3
68379pyrazinamidase-3.5.1.B15
117205oxidase-
117205beta-galactosidase+3.2.1.23
117205alcohol dehydrogenase-1.1.1.1
117205catalase-1.11.1.6
117205gamma-glutamyltransferase-2.3.2.2
117205lysine decarboxylase-4.1.1.18
117205ornithine decarboxylase-4.1.1.17

API coryne

@refNITPYZPYRAPALbeta GURbeta GALalpha GLUbeta NAGESCUREGELControlRIBXYLMANMALLACSACGLYGCAT
46854--+-+++++---++++++--

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
117205--++-+---++++++-+---

API 50CHac

@refGLYERYDARALARARIBDXYLLXYLADOMDXGALGLUFRUMNESBERHADULINOMANSORMDMMDGNAGAMYARBESCSALCELMALLACMELSACTREINUMLZRAFAMDGLYGXLTGENTURLYXTAGDFUCLFUCDARLLARLGNT2KG5KG
117205+--+++---++++-+--++++++++++++++++-++--++----+/--+-+/-

API rID32STR

@refADH Argbeta GLUbeta GARbeta GURalpha GALPALRIBMANSORLACTRERAFSACLARADARLCDEXVPAPPAbeta GALPyrAbeta NAGGTAHIPGLYGPULMALMELMLZMbeta DGTAGbeta MANURE
9002-++-+-++-+++++--+-++-+---++-+-+-
9002-++/--+-++-+++++--+-++-+---++-+-+-
9002++--+-++-+++++--+-++++---+++/-+-+-
9002+++-+-++-+++++--+-++++---++++-+-

API biotype100

@refGLUFRUGALTREMNESBEMELSACRAFMTEMALLACLTEMbGaMaGaCELGENMbGuESCRIBARAXYLPLERHAFUCMLZDARLLARLXLTDULTAGGLYINOMANMTLTURSORADOHBGLYXERYMDG3MDGSATMUCLTATDTATMTATDMLTLMLTCATETATETTECITGRTGAT2KG5KGTRYNAGGNTPACPATpOBEQATGTEmOBEBATPPATCMTTGEBETPCEABTHINLATCAPCYTHISSUCFUMGREGYTAVTETNTTNGLNITA3OBUAPTGTTPRODALALALASERMNTPROPTYR2KT
117205---------------------------------------------------------------------------------------------------

Isolation, sampling and environmental information

isolation

@refsample typesampling date
9002plant material
46854Plant material1984
67770Plant material
117205Plant

isolation source categories

  • Cat1: #Host
  • Cat2: #Plants

taxonmaps

  • @ref: 69479
  • File name: preview.99_759.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_13;96_19;97_19;98_605;99_759&stattab=map
  • Last taxonomy: Enterococcus
  • 16S sequence: Y18161
  • Sequence Identity:
  • Total samples: 18861
  • soil counts: 573
  • aquatic counts: 1598
  • animal counts: 15794
  • plant counts: 896

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
90022Risk group (German classification)
1172052Risk group (French classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Enterococcus casseliflavus 16S ribosomal RNA gene, partial sequenceAF0399031509ena37734
20218Enterococcus casseliflavus strain ATCC 25788 16S ribosomal RNA gene, partial sequenceKC510229814ena37734
20218E.casseliflavus 16S rRNA gene, partial (strain ATTC 25788 (CCUG 18657))Y12908366ena37734
20218Enterococcus casseliflavus strain CCUG 18657 clone 1 16S-23S ribosomal RNA intergenic spacer, partial sequenceDQ204549226ena37734
20218Enterococcus casseliflavus 16S rRNA gene, strain CECT969TAJ4208041451ena37734
20218Enterococcus casseliflavus 16S rRNA gene, strain LMG 10745AJ3018261904ena37734
20218Enterococcus casseliflavus 16S rRNA gene, strain NCIMB 11449Y181611421ena37734
9002Enterococcus casseliflavus gene for 16S rRNA, partial sequence, strain: NBRC 100478AB6811761425ena37734

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Enterococcus casseliflavus NCTC12361GCA_900447665contigncbi37734
66792Enterococcus casseliflavus DSM 20680GCA_001885845scaffoldncbi37734
66792Enterococcus casseliflavus NBRC 100478GCA_001544095contigncbi1218087
66792Enterococcus casseliflavus NBRC 1004781218087.3wgspatric1218087
66792Enterococcus casseliflavus strain DSM 2068037734.14wgspatric37734
66792Enterococcus casseliflavus strain FDAARGOS_112037734.151completepatric37734
66792Enterococcus casseliflavus strain NCTC1236137734.39wgspatric37734
66792Enterococcus casseliflavus NBRC 1004782731957559draftimg1218087

GC content

  • @ref: 9002
  • GC-content: 43.4
  • method: thermal denaturation, midpoint method (Tm)

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingno67no
gram-positiveyes90.234no
anaerobicno96.793yes
halophileyes79.699no
spore-formingno82.308no
glucose-utilyes87.695no
aerobicno95.941yes
motileno85.209no
flagellatedno93.732no
thermophileno99.262no
glucose-fermentyes88.565no

External links

@ref: 9002

culture collection no.: DSM 20680, ATCC 25788, CCM 2478, NCDO 2372, NCIB 11449, CCUG 18657, NCTC 12361, JCM 8723, CECT 969, CGMCC 1.9124, CIP 103018, KCTC 3638, LMG 10745, NBRC 100478, NCIMB 11449, NRRL B-3502, VTT E-97775

straininfo link

  • @ref: 74766
  • straininfo: 363642

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny1939593Vancomycin susceptibility and identification of motile enterococci.Vincent S, Knight RG, Green M, Sahm DF, Shlaes DMJ Clin Microbiol10.1128/jcm.29.10.2335-2337.19911991*Bacterial Proteins, Carrier Proteins/metabolism, DNA, Bacterial/genetics/isolation & purification, Drug Resistance, Microbial, Enterococcus/*drug effects/isolation & purification/metabolism, Gram-Positive Bacterial Infections/microbiology, *Hexosyltransferases, Humans, Muramoylpentapeptide Carboxypeptidase/metabolism, Penicillin-Binding Proteins, *Peptidyl Transferases, Sequence Homology, Nucleic Acid, Species Specificity, Vancomycin/*pharmacologyMetabolism
Phylogeny7699030DNA hybridization and contour-clamped homogeneous electric field electrophoresis for identification of enterococci to the species level.Donabedian S, Chow JW, Shlaes DM, Green M, Zervos MJJ Clin Microbiol10.1128/jcm.33.1.141-145.19951995Blotting, Southern, DNA, Bacterial/*genetics, Electrophoresis, Agar Gel/methods, Enterococcus/*classification/genetics, Gram-Negative Bacterial Infections/microbiology, Humans, Nucleic Acid Hybridization, Species SpecificityEnzymology
Phylogeny7960141Resistance of Enterococcus faecium to neutrophil-mediated phagocytosis.Arduino RC, Jacques-Palaz K, Murray BE, Rakita RMInfect Immun10.1128/iai.62.12.5587-5594.19941994Bacterial Capsules/*chemistry, Enterococcus faecium/classification/*immunology/pathogenicity/ultrastructure, Humans, Microscopy, Electron, Microscopy, Fluorescence, N-Acetylneuraminic Acid, Neutrophils/*immunology/ultrastructure, Opsonin Proteins, *Phagocytosis, Sialic Acids/analysis, Species Specificity, VirulencePathogenicity
Enzymology7986009Analysis of genes encoding D-alanine-D-alanine ligase-related enzymes in Enterococcus casseliflavus and Enterococcus flavescens.Navarro F, Courvalin PAntimicrob Agents Chemother10.1128/AAC.38.8.17881994Amino Acid Sequence, Bacterial Proteins/genetics, Base Sequence, Drug Resistance, Microbial, Enterococcus/*genetics, *Genes, Bacterial, Molecular Sequence Data, Peptide Synthases/*genetics, Vancomycin/pharmacologyGenetics
Enzymology9294159Bacterial resistance to vancomycin: overproduction, purification, and characterization of VanC2 from Enterococcus casseliflavus as a D-Ala-D-Ser ligase.Park IS, Lin CH, Walsh CTProc Natl Acad Sci U S A10.1073/pnas.94.19.100401997*Bacterial Proteins, Cloning, Molecular, Drug Resistance, Microbial/genetics, Enterococcus/*enzymology, Escherichia coli/genetics, Peptide Synthases/*genetics/isolation & purification/metabolism, Vancomycin/*pharmacologyBiotechnology
Metabolism12234834Biochemical and genetic characterization of the vanC-2 vancomycin resistance gene cluster of Enterococcus casseliflavus ATCC 25788.Dutta I, Reynolds PEAntimicrob Agents Chemother10.1128/AAC.46.10.3125-3132.20022002*Bacterial Proteins, Cloning, Molecular, Enterococcus/*drug effects/genetics/growth & development, *Gene Expression Regulation, Bacterial, Microbial Sensitivity Tests, Molecular Sequence Data, *Multigene Family, Peptide Synthases/*genetics/*metabolism, Peptidoglycan/metabolism, Plasmids/genetics, Sequence Analysis, DNA, Serine/metabolism, Vancomycin Resistance/*geneticsEnzymology
Enzymology12403118Nucleotide substitutions in vanC-2 gene of Enterococcus casseliflavus isolates obtained from chickens.Murase T, Mito Y, Otsuki K, Suzuki R, Yamai SEpidemiol Infect10.1017/s09502688020075012002Animals, *Bacterial Proteins, Base Sequence, Brazil/epidemiology, Chickens/*microbiology, China/epidemiology, DNA, Bacterial/*genetics, Electrophoresis, Gel, Pulsed-Field, Enterococcus/*genetics/isolation & purification, Food Contamination, *Food Microbiology, Gram-Positive Bacterial Infections/*prevention & control, Humans, Japan/epidemiology, Molecular Sequence Data, Peptide Synthases/*geneticsBiotechnology
Enzymology12615874The vanC-3 vancomycin resistance gene cluster of Enterococcus flavescens CCM 439.Dutta I, Reynolds PEJ Antimicrob Chemother10.1093/jac/dkg1312003Bacterial Proteins/*genetics/isolation & purification, Enterococcus/*genetics/isolation & purification, Humans, Molecular Sequence Data, Multigene Family/*genetics, Phenotype, Polymerase Chain Reaction, Polymorphism, Restriction Fragment Length, Vancomycin Resistance/*geneticsPhylogeny
Phylogeny34919037Enterococcus innesii sp. nov., isolated from the wax moth Galleria mellonella.Gooch HCC, Kiu R, Rudder S, Baker DJ, Hall LJ, Maxwell AInt J Syst Evol Microbiol10.1099/ijsem.0.0051682021Animals, Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Enterococcus/*classification/isolation & purification, Fatty Acids/chemistry, *Moths/microbiology, Nucleic Acid Hybridization, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNATranscriptome
Phylogeny35456876Enterococcus casseliflavus KB1733 Isolated from a Traditional Japanese Pickle Induces Interferon-Lambda Production in Human Intestinal Epithelial Cells.Satomi S, Kokubu D, Inoue T, Sugiyama M, Mizokami M, Suzuki S, Murata KMicroorganisms10.3390/microorganisms100408272022Biotechnology

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
9002Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 20680)https://www.dsmz.de/collection/catalogue/details/culture/DSM-20680
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
38111Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/14769
46854Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 18657)https://www.ccug.se/strain?id=18657
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68371Automatically annotated from API 50CH acid
68379Automatically annotated from API Coryne
68381Automatically annotated from API rID32STR
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
74766Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID363642.1StrainInfo: A central database for resolving microbial strain identifiers
117205Curators of the CIPCollection of Institut Pasteur (CIP 103018)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20103018