Strain identifier

BacDive ID: 5167

Type strain: No

Species: Serratia marcescens

Strain Designation: KRED

Strain history: CIP <- 2002, R. Iriye, Shinshu Univ., Nagano, Japan: strain KRED

NCBI tax ID(s): 615 (species)

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General

@ref: 6808

BacDive-ID: 5167

DSM-Number: 17174

keywords: genome sequence, 16S sequence, Bacteria, aerobe, mesophilic, Gram-negative, motile, rod-shaped

description: Serratia marcescens KRED is an aerobe, mesophilic, Gram-negative bacterium that was isolated from activated sludge of waste water treatment tank.

NCBI tax id

  • NCBI tax id: 615
  • Matching level: species

strain history

@refhistory
6808<- R. Iriye, Shinsu University, Japan
421062002, R. Iriye, Shinshu Univ., Nagano, Japan: strain KRED
67770R. Iriye strain KRED.
120786CIP <- 2002, R. Iriye, Shinshu Univ., Nagano, Japan: strain KRED

doi: 10.13145/bacdive5167.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Gammaproteobacteria
  • order: Enterobacterales
  • family: Yersiniaceae
  • genus: Serratia
  • species: Serratia marcescens
  • full scientific name: Serratia marcescens Bizio 1823 (Approved Lists 1980)

  • synonyms

    • @ref: 20215
    • synonym: Serratia marcescens subsp. sakuensis

@ref: 6808

domain: Bacteria

phylum: Proteobacteria

class: Gammaproteobacteria

order: Enterobacteriales, not validated

family: Yersiniaceae

genus: Serratia

species: Serratia marcescens subsp. sakuensis

full scientific name: Serratia marcescens subsp. sakuensis Ajithkumar et al. 2003

strain designation: KRED

type strain: no

Morphology

cell morphology

  • @ref: 120786
  • gram stain: negative
  • cell shape: rod-shaped
  • motility: yes

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
6808CASO AGAR (MERCK 105458) (DSMZ Medium 220)yeshttps://mediadive.dsmz.de/medium/220Name: CASO AGAR (Merck 105458) (DSMZ Medium 220) Composition: Agar 15.0 g/l Casein peptone 15.0 g/l NaCl 5.0 g/l Soy peptone 5.0 g/l Distilled water
42106MEDIUM 72- for trypto casein soja agaryesDistilled water make up to (1000.000 ml);Trypto casein soy agar (40.000 g)
120786CIP Medium 72yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=72

culture temp

@refgrowthtypetemperaturerange
6808positivegrowth28mesophilic
42106positivegrowth30mesophilic
60049positivegrowth30-37mesophilic
67770positivegrowth30mesophilic
120786positivegrowth10-41
120786nogrowth5psychrophilic

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
60049aerobe
120786facultative anaerobe

observation

  • @ref: 67770
  • observation: quinones: Q-8

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
12078629864mannitol+fermentation
12078616947citrate+carbon source
12078617234glucose+fermentation
12078617716lactose-fermentation
12078617632nitrate+reduction
12078616301nitrite-reduction
12078615792malonate-assimilation
120786132112sodium thiosulfate-builds gas from
12078617234glucose+degradation

antibiotic resistance

  • @ref: 120786
  • metabolite: 0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)
  • is antibiotic: yes
  • is sensitive: no
  • is resistant: yes

metabolite production

  • @ref: 120786
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

metabolite tests

@refChebi-IDmetabolitevoges-proskauer-testmethylred-test
12078615688acetoin+
12078617234glucose-

enzymes

@refvalueactivityec
68382alpha-fucosidase-3.2.1.51
68382alpha-mannosidase-3.2.1.24
68382N-acetyl-beta-glucosaminidase+3.2.1.52
68382beta-glucosidase+3.2.1.21
68382alpha-glucosidase+3.2.1.20
68382beta-glucuronidase-3.2.1.31
68382beta-galactosidase+3.2.1.23
68382alpha-galactosidase-3.2.1.22
68382naphthol-AS-BI-phosphohydrolase+
68382acid phosphatase+3.1.3.2
68382alpha-chymotrypsin-3.4.21.1
68382trypsin+3.4.21.4
68382cystine arylamidase-3.4.11.3
68382valine arylamidase-
68382leucine arylamidase+3.4.11.1
68382lipase (C 14)+
68382esterase lipase (C 8)+
68382esterase (C 4)+
68382alkaline phosphatase+3.1.3.1
120786oxidase-
120786beta-galactosidase+3.2.1.23
120786alcohol dehydrogenase-1.1.1.1
120786gelatinase+
120786caseinase+3.4.21.50
120786catalase+1.11.1.6
120786tween esterase+
120786lysine decarboxylase+4.1.1.18
120786ornithine decarboxylase+4.1.1.17
120786phenylalanine ammonia-lyase-4.3.1.24
120786tryptophan deaminase-
120786urease-3.5.1.5

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
120786-+++++--+-++-+-+++--

API biotype100

@refGLUFRUGALTREMNESBEMELSACRAFMTEMALLACLTEMbGaMaGaCELGENMbGuESCRIBARAXYLPLERHAFUCMLZDARLLARLXLTDULTAGGLYINOMANMTLTURSORADOHBGLYXERYMDG3MDGSATMUCLTATDTATMTATDMLTLMLTCATETATETTECITGRTGAT2KG5KGTRYNAGGNTPACPATpOBEQATGTEmOBEBATPPATCMTTGEBETPCEABTHINLATCAPCYTHISSUCFUMGREGYTAVTETNTTNGLNITA3OBUAPTGTTPRODALALALASERMNTPROPTYR2KT
120786+++++--+-++------+++----+--++--+++--++-++--------+++-+++++-+++++-+------++-+++-++-+---+-+++++++--+-

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinentisolation date
6808activated sludge of waste water treatment tankSaku, NaganoJapanJPNAsia
60049Water,domestic wastewater treatment tankNagano,Saku,KomabaJapanJPNAsia
67770Suspended water of a domestic wastewater treatment tankKomaba, Saku, Nagano Pref.JapanJPNAsia
120786Environment, Domestic wastewater treatment tankSakuJapanJPNAsia1992

isolation source categories

Cat1Cat2Cat3
#Engineered#Built environment#Container (Reservoir)
#Engineered#Waste#Activated sludge
#Engineered#Waste#Wastewater

taxonmaps

  • @ref: 69479
  • File name: preview.99_289.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_6;96_7;97_7;98_244;99_289&stattab=map
  • Last taxonomy: Serratia marcescens
  • 16S sequence: AB061685
  • Sequence Identity:
  • Total samples: 22704
  • soil counts: 1270
  • aquatic counts: 2336
  • animal counts: 18103
  • plant counts: 995

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
68082Risk group (German classification)
1207861Risk group (French classification)

Sequence information

16S sequences

  • @ref: 6808
  • description: Serratia marcescens gene for 16S rRNA, partial sequence
  • accession: AB061685
  • length: 1532
  • database: ena
  • NCBI tax ID: 211760

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Serratia marcescens subsp. sakuensis strain DSM 17174211760.18wgspatric615
67770Serratia marcescens DSM 17174GCA_004684145contigncbi615

GC content

@refGC-contentmethod
680858
6777058high performance liquid chromatography (HPLC)

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
motileyes90.247no
gram-positiveno98.086no
anaerobicno98.041yes
aerobicyes90.344yes
halophileno92.733no
spore-formingno94.989no
glucose-utilyes94.972no
flagellatedno60.723no
thermophileno99.383yes
glucose-fermentyes91.438no

External links

@ref: 6808

culture collection no.: DSM 17174, CIP 107489, JCM 11315, CCUG 53879, CCM 7122

straininfo link

  • @ref: 74659
  • straininfo: 100951

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny12656181Spore-forming Serratia marcescens subsp. sakuensis subsp. nov., isolated from a domestic wastewater treatment tank.Ajithkumar B, Ajithkumar VP, Iriye R, Doi Y, Sakai TInt J Syst Evol Microbiol10.1099/ijs.0.02158-02003DNA, Bacterial/genetics, DNA, Ribosomal/genetics, Drug Resistance, Bacterial, Fatty Acids/analysis, Hot Temperature, Japan, Microscopy, Electron, Molecular Sequence Data, Phylogeny, Pigments, Biological/analysis, Quinones/analysis, RNA, Bacterial/genetics, RNA, Ribosomal, 16S/genetics, Serratia marcescens/*classification/genetics/*isolation & purification/physiology, Sewage/*microbiology, Spores, Bacterial/isolation & purificationStress
Phylogeny19578149Serratia nematodiphila sp. nov., associated symbiotically with the entomopathogenic nematode Heterorhabditidoides chongmingensis (Rhabditida: Rhabditidae).Zhang CX, Yang SY, Xu MX, Sun J, Liu H, Liu JR, Liu H, Kan F, Sun J, Lai R, Zhang KYInt J Syst Evol Microbiol10.1099/ijs.0.65718-02009Animals, Bacterial Typing Techniques, Base Composition, DNA, Bacterial/analysis, Fatty Acids/analysis, Fluorescence, Genes, rRNA, Genotype, Intestines/microbiology, Molecular Sequence Data, Nucleic Acid Hybridization, Phenotype, Phylogeny, RNA, Ribosomal, 16S/genetics, Rhabditoidea/*microbiology, Sequence Analysis, DNA, Serratia/*classification/genetics/isolation & purification/physiology, Species Specificity, *SymbiosisGenetics
Phylogeny31490113Genome-based analyses indicate that Serratia marcescens subsp. marcescens and Serratia marcescens subsp. sakuensis do not merit separation to subspecies status.Doijad S, Chakraborty TInt J Syst Evol Microbiol10.1099/ijsem.0.0037062019Bacterial Typing Techniques, DNA, Bacterial/genetics, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Serratia marcescens/*classificationTranscriptome

Reference

@idauthorscataloguedoi/urltitle
6808Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 17174)https://www.dsmz.de/collection/catalogue/details/culture/DSM-17174
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
42106Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/4930
60049Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 53879)https://www.ccug.se/strain?id=53879
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
74659Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID100951.1StrainInfo: A central database for resolving microbial strain identifiers
120786Curators of the CIPCollection of Institut Pasteur (CIP 107489)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20107489