Strain identifier

BacDive ID: 5084

Type strain: Yes

Species: Providencia stuartii

Strain Designation: 2896-68

Strain history: CIP <- 1996, LMG <- 1987, NCTC <- 1984, ATCC <- CDC: strain 2896-68 <- Colorado State Hlth. Dept. Human, USA

NCBI tax ID(s): 588 (species)

« back to strain detail view
For citation purpose refer to the digital object identifier (doi) of the current version.
Archive
version 8.1 (current version):
version 8:
version 7.1:
version 7:
version 6:
version 5:
version 4.1:
version 4:
version 3:
version 2.1:
version 2:
version 1:
version 8.1 (current version)

General

@ref: 1720

BacDive-ID: 5084

DSM-Number: 4539

keywords: genome sequence, 16S sequence, Bacteria, facultative anaerobe, mesophilic, Gram-negative, rod-shaped

description: Providencia stuartii 2896-68 is a facultative anaerobe, mesophilic, Gram-negative bacterium that was isolated from Human.

NCBI tax id

  • NCBI tax id: 588
  • Matching level: species

strain history

@refhistory
1720<- ATCC <- CDC, 2896-68
67770R. Sakazaki 416 <-- ATCC 29914 <-- CDC 2896-68 <-- Colorado State Health Dept., USA.
123329CIP <- 1996, LMG <- 1987, NCTC <- 1984, ATCC <- CDC: strain 2896-68 <- Colorado State Hlth. Dept. Human, USA

doi: 10.13145/bacdive5084.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Gammaproteobacteria
  • order: Enterobacterales
  • family: Morganellaceae
  • genus: Providencia
  • species: Providencia stuartii
  • full scientific name: Providencia stuartii (Buttiaux et al. 1954) Ewing 1962 (Approved Lists 1980)

  • synonyms

    • @ref: 20215
    • synonym: Proteus stuartii

@ref: 1720

domain: Bacteria

phylum: Proteobacteria

class: Gammaproteobacteria

order: Enterobacteriales, not validated

family: Morganellaceae

genus: Providencia

species: Providencia stuartii

full scientific name: Providencia stuartii (Buttiaux et al. 1954) Ewing 1962

strain designation: 2896-68

type strain: yes

Morphology

cell morphology

  • @ref: 123329
  • gram stain: negative
  • cell shape: rod-shaped
  • motility: no

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
1720COLUMBIA BLOOD MEDIUM (DSMZ Medium 693)yeshttps://mediadive.dsmz.de/medium/693Name: COLUMBIA BLOOD MEDIUM (DSMZ Medium 693) Composition: Defibrinated sheep blood 50.0 g/l Columbia agar base
1720TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92)yeshttps://mediadive.dsmz.de/medium/92Name: TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92) Composition: Trypticase soy broth 30.0 g/l Agar 15.0 g/l Yeast extract 3.0 g/l Distilled water
37044MEDIUM 3 - Columbia agaryesColumbia agar (39.000 g);distilled water (1000.000 ml)
123329CIP Medium 3yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=3
123329CIP Medium 72yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=72

culture temp

@refgrowthtypetemperaturerange
1720positivegrowth30mesophilic
37044positivegrowth30mesophilic
67770positivegrowth37mesophilic
123329positivegrowth10-41
123329nogrowth5psychrophilic

Physiology and metabolism

oxygen tolerance

  • @ref: 123329
  • oxygen tolerance: facultative anaerobe

metabolite utilization

@refmetaboliteutilization activitykind of utilization testedChebi-ID
68371Potassium 5-ketogluconate-builds acid from
68371Potassium 2-ketogluconate-builds acid from
68371gluconate+builds acid from24265
68371L-arabitol-builds acid from18403
68371D-arabitol-builds acid from18333
68371L-fucose-builds acid from18287
68371D-fucose-builds acid from28847
68371D-tagatose-builds acid from16443
68371D-lyxose+builds acid from62318
68371turanose-builds acid from32528
68371gentiobiose-builds acid from28066
68371xylitol+builds acid from17151
68371glycogen-builds acid from28087
68371starch-builds acid from28017
68371raffinose-builds acid from16634
68371melezitose-builds acid from6731
68371inulin-builds acid from15443
68371sucrose-builds acid from17992
68371melibiose-builds acid from28053
68371lactose-builds acid from17716
68371maltose-builds acid from17306
68371cellobiose-builds acid from17057
68371salicin-builds acid from17814
68371esculin-builds acid from4853
68371arbutin-builds acid from18305
68371amygdalin-builds acid from27613
68371N-acetylglucosamine+builds acid from59640
68371methyl alpha-D-glucopyranoside-builds acid from320061
68371methyl alpha-D-mannoside-builds acid from43943
68371D-sorbitol-builds acid from17924
68371D-mannitol-builds acid from16899
68371myo-inositol+builds acid from17268
68371galactitol-builds acid from16813
68371L-rhamnose-builds acid from62345
68371L-sorbose-builds acid from17266
68371D-mannose+builds acid from16024
68371D-fructose+builds acid from15824
68371D-glucose+builds acid from17634
68371D-galactose+builds acid from12936
68371methyl beta-D-xylopyranoside-builds acid from74863
68371ribitol-builds acid from15963
68371L-xylose-builds acid from65328
68371D-xylose-builds acid from65327
68371D-ribose+builds acid from16988
68371L-arabinose-builds acid from30849
68371D-arabinose-builds acid from17108
68371erythritol-builds acid from17113
68371glycerol+builds acid from17754
68368L-arabinose-fermentation30849
68368amygdalin-fermentation27613
68368melibiose-fermentation28053
68368sucrose-fermentation17992
68368L-rhamnose-fermentation62345
68368sorbitol-fermentation30911
68368myo-inositol+fermentation17268
68368D-mannitol-fermentation16899
68368D-glucose+fermentation17634
68368gelatin-hydrolysis5291
68368tryptophan+energy source27897
68368urea-hydrolysis16199
68368citrate+assimilation16947
68368ornithine-degradation18257
68368lysine-degradation25094
68368arginine-hydrolysis29016
123329mannitol-fermentation29864
123329citrate+carbon source16947
123329glucose+fermentation17234
123329lactose-fermentation17716
123329nitrate+reduction17632
123329nitrite-reduction16301
123329malonate-assimilation15792
123329sodium thiosulfate-builds gas from132112

metabolite production

@refChebi-IDmetaboliteproduction
6836815688acetoinno
6836835581indoleyes
6836816136hydrogen sulfideno
12332935581indoleyes

metabolite tests

@refChebi-IDmetabolitevoges-proskauer-testindole testmethylred-test
6836815688acetoin-
6836835581indole+
12332915688acetoin-
12332917234glucose+

enzymes

@refvalueactivityec
68382alpha-fucosidase-3.2.1.51
68382alpha-mannosidase-3.2.1.24
68382N-acetyl-beta-glucosaminidase+3.2.1.52
68382beta-glucosidase-3.2.1.21
68382alpha-glucosidase-3.2.1.20
68382beta-glucuronidase-3.2.1.31
68382beta-galactosidase-3.2.1.23
68382alpha-galactosidase-3.2.1.22
68382naphthol-AS-BI-phosphohydrolase+
68382acid phosphatase+3.1.3.2
68382alpha-chymotrypsin-3.4.21.1
68382trypsin+3.4.21.4
68382cystine arylamidase-3.4.11.3
68382valine arylamidase+
68382leucine arylamidase+3.4.11.1
68382lipase (C 14)-
68382esterase lipase (C 8)-
68382esterase (C 4)-
68382alkaline phosphatase+3.1.3.1
68368cytochrome oxidase-1.9.3.1
68368gelatinase-
68368tryptophan deaminase+4.1.99.1
68368urease-3.5.1.5
68368ornithine decarboxylase-4.1.1.17
68368lysine decarboxylase-4.1.1.18
68368arginine dihydrolase-3.5.3.6
68368beta-galactosidase-3.2.1.23
123329oxidase-
123329beta-galactosidase-3.2.1.23
123329alcohol dehydrogenase-1.1.1.1
123329gelatinase-
123329catalase+1.11.1.6
123329lysine decarboxylase-4.1.1.18
123329ornithine decarboxylase-4.1.1.17
123329tryptophan deaminase+
123329urease+3.5.1.5

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
123329-+---++-+-++-----+--

API 20E

@refONPGADH ArgLDC LysODCCITH2SURETDA TrpINDVPGELGLUMANINOSorRHASACMELAMYARAOX
1720----+--++--+-+-------

API 50CHac

@refGLYERYDARALARARIBDXYLLXYLADOMDXGALGLUFRUMNESBERHADULINOMANSORMDMMDGNAGAMYARBESCSALCELMALLACMELSACTREINUMLZRAFAMDGLYGXLTGENTURLYXTAGDFUCLFUCDARLLARLGNT2KG5KG
123329+---+----++++---+----+---------+/------+--+-----+--

API biotype100

@refGLUFRUGALTREMNESBEMELSACRAFMTEMALLACLTEMbGaMaGaCELGENMbGuESCRIBARAXYLPLERHAFUCMLZDARLLARLXLTDULTAGGLYINOMANMTLTURSORADOHBGLYXERYMDG3MDGSATMUCLTATDTATMTATDMLTLMLTCATETATETTECITGRTGAT2KG5KGTRYNAGGNTPACPATpOBEQATGTEmOBEBATPPATCMTTGEBETPCEABTHINLATCAPCYTHISSUCFUMGREGYTAVTETNTTNGLNITA3OBUAPTGTTPRODALALALASERMNTPROPTYR2KT
123329+++++--------------+--------+--++------+--------++++-+-----+++-----------------++-----+--++++++--++

Isolation, sampling and environmental information

isolation

@refsample type
46151Human
67770Human

isolation source categories

  • Cat1: #Host
  • Cat2: #Human

taxonmaps

  • @ref: 69479
  • File name: preview.99_571.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_6;96_362;97_407;98_466;99_571&stattab=map
  • Last taxonomy: Providencia stuartii
  • 16S sequence: NR_114964
  • Sequence Identity:
  • Total samples: 11465
  • soil counts: 839
  • aquatic counts: 1360
  • animal counts: 8405
  • plant counts: 861

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
17202Risk group (German classification)
1233292Risk group (French classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Providencia stuartii strain ATCC 29914 16S ribosomal RNA gene, partial sequenceAF0085811494ena588
20218Providencia stuartii strain ATCC 29914 16S ribosomal RNA gene, partial sequenceFJ971875795ena588
20218Providencia stuartii 16S rRNA gene, type strain DSM 4539AM0404911478ena588
20218Providencia stuartii strain DSM 4539 16S ribosomal RNA gene, partial sequenceEU587024978ena588
1720Providencia stuartii strain ATCC 29914 16S ribosomal RNA, partial sequenceNR_0248481494nuccore588
1720Providencia stuartii strain DSM 4539 16S ribosomal RNA, partial sequenceNR_1149641478nuccore588

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Providencia stuartii strain NCTC11800588.51wgspatric588
67770Providencia stuartii NCTC11800GCA_900455155contigncbi588

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
gram-positiveno98.436no
anaerobicno98.047no
halophileno86.848no
spore-formingno95.945no
glucose-utilyes92.299no
thermophileno97.569yes
flagellatedyes59.83no
aerobicyes79.313no
motileyes86.602no
glucose-fermentyes88.597yes

External links

@ref: 1720

culture collection no.: DSM 4539, ATCC 29914, CCUG 14805, CECT 866, CDC 2896-68, JCM 24298, CIP 104687, LMG 3260, NCTC 11800, LMG 7567

straininfo link

  • @ref: 74579
  • straininfo: 92374

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny2903175Detection of genomic variation in Providencia stuartii clinical isolates by analysis of DNA restriction fragment length polymorphisms containing rRNA cistrons.Owen RJ, Beck A, Dayal PA, Dawson CJ Clin Microbiol10.1128/jcm.26.10.2161-2166.19881988DNA Probes, DNA, Bacterial/*analysis, DNA, Ribosomal/*analysis, *Genes, Humans, Nucleotide Mapping, *Polymorphism, Genetic, *Polymorphism, Restriction Fragment Length, Proteus/*genetics, Providencia/*genetics, RNA, Ribosomal/*geneticsPathogenicity
Phylogeny19406801Providencia sneebia sp. nov. and Providencia burhodogranariea sp. nov., isolated from wild Drosophila melanogaster.Juneja P, Lazzaro BPInt J Syst Evol Microbiol10.1099/ijs.0.000117-02009Animals, Bacterial Proteins/genetics, Bacterial Typing Techniques, DNA, Bacterial/analysis, DNA, Ribosomal/analysis, Drosophila melanogaster/*microbiology, Genes, rRNA, Molecular Sequence Data, Nucleic Acid Hybridization, Phenotype, Phylogeny, Providencia/*classification/genetics/isolation & purification/physiology, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Species SpecificityGenetics
Metabolism20667831Implication of porins in beta-lactam resistance of Providencia stuartii.Tran QT, Mahendran KR, Hajjar E, Ceccarelli M, Davin-Regli A, Winterhalter M, Weingart H, Pages JMJ Biol Chem10.1074/jbc.M110.1433052010Amino Acid Sequence, Anti-Bacterial Agents/*pharmacology, Bacterial Outer Membrane Proteins/*metabolism, *Drug Resistance, Bacterial, Humans, Microbial Sensitivity Tests, Molecular Sequence Data, Porins/*metabolism, Providencia/cytology/*drug effects/*metabolism, Sequence Alignment, beta-Lactams/*pharmacologyPathogenicity

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
1720Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 4539)https://www.dsmz.de/collection/catalogue/details/culture/DSM-4539
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
37044Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/16623
46151Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 14805)https://www.ccug.se/strain?id=14805
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68368Automatically annotated from API 20E
68371Automatically annotated from API 50CH acid
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
74579Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID92374.1StrainInfo: A central database for resolving microbial strain identifiers
123329Curators of the CIPCollection of Institut Pasteur (CIP 104687)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20104687