Strain identifier

BacDive ID: 4981

Type strain: Yes

Species: Leminorella grimontii

Strain Designation: 81H-380

Strain history: CIP <- 1989, NCTC <- 1986, ATCC <- F. Brenner: strain 81H-380 (= CDC 1944-81)

NCBI tax ID(s): 1005999 (strain), 82981 (species)

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General

@ref: 1925

BacDive-ID: 4981

DSM-Number: 5078

keywords: genome sequence, 16S sequence, Bacteria, mesophilic, Gram-negative, human pathogen

description: Leminorella grimontii 81H-380 is a mesophilic, Gram-negative human pathogen that was isolated from stool of seven month old female with gastroenteritis.

NCBI tax id

NCBI tax idMatching level
82981species
1005999strain

strain history

@refhistory
1925<- ATCC <- F. Brenner, 81H-380
67770Y. Kosako 85568 <-- R. Sakazaki 85030 <-- CDC 1944-81.
119743CIP <- 1989, NCTC <- 1986, ATCC <- F. Brenner: strain 81H-380 (= CDC 1944-81)

doi: 10.13145/bacdive4981.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Gammaproteobacteria
  • order: Enterobacterales
  • family: Budviciaceae
  • genus: Leminorella
  • species: Leminorella grimontii
  • full scientific name: Leminorella grimontii Hickman-Brenner et al. 1985

@ref: 1925

domain: Bacteria

phylum: Proteobacteria

class: Gammaproteobacteria

order: Enterobacteriales, not validated

family: Budviciaceae

genus: Leminorella

species: Leminorella grimontii

full scientific name: Leminorella grimontii Hickman-Brenner et al. 1985

strain designation: 81H-380

type strain: yes

Morphology

cell morphology

  • @ref: 69480
  • gram stain: negative
  • confidence: 99.982

multimedia

@refmultimedia contentcaptionintellectual property rights
66793EM_DSM_5078_1.jpgelectron microscopic image© HZI/Manfred Rohde
66793EM_DSM_5078_2.jpgelectron microscopic image© HZI/Manfred Rohde
66793EM_DSM_5078_3.jpgelectron microscopic image© HZI/Manfred Rohde
66793EM_DSM_5078_4.jpgelectron microscopic image© HZI/Manfred Rohde

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
1925NUTRIENT AGAR (DSMZ Medium 1)yeshttps://mediadive.dsmz.de/medium/1Name: NUTRIENT AGAR (DSMZ Medium 1) Composition: Agar 15.0 g/l Peptone 5.0 g/l Meat extract 3.0 g/l Distilled water
34021MEDIUM 3 - Columbia agaryesColumbia agar (39.000 g);distilled water (1000.000 ml)
119743CIP Medium 3yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=3

culture temp

@refgrowthtypetemperaturerange
1925positivegrowth37mesophilic
34021positivegrowth30mesophilic
67770positivegrowth37mesophilic

Physiology and metabolism

spore formation

@refspore formationconfidence
69481no100
69480no99.923

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
6837418257ornithine-degradation
6837429016arginine-hydrolysis
6837425094lysine-degradation
6837416199urea-hydrolysis
6837418403L-arabitol-builds acid from
6837418024D-galacturonic acid-builds acid from
68374Potassium 5-ketogluconate-builds acid from
6837416899D-mannitol-builds acid from
6837417306maltose-builds acid from
6837415963ribitol-builds acid from
6837418394palatinose-builds acid from
6837415792malonate-assimilation
6837427897tryptophan-energy source
6837417634D-glucose+builds acid from
6837417992sucrose-builds acid from
6837430849L-arabinose+builds acid from
6837418333D-arabitol-builds acid from
6837427082trehalose-builds acid from
6837462345L-rhamnose-builds acid from
6837417268myo-inositol-builds acid from
6837417057cellobiose-builds acid from
6837430911sorbitol-builds acid from

metabolite production

  • @ref: 68374
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

metabolite tests

  • @ref: 68374
  • Chebi-ID: 35581
  • metabolite: indole
  • indole test: -

enzymes

@refvalueactivityec
68374L-aspartate arylamidase-3.4.11.21
68374alpha-maltosidase-
68374alpha-galactosidase-3.2.1.22
68374alpha-glucosidase-3.2.1.20
68374beta-galactosidase-3.2.1.23
68374N-acetyl-beta-glucosaminidase+3.2.1.52
68374beta-glucuronidase-3.2.1.31
68374beta-glucosidase-3.2.1.21
68374lipase-
68374urease-3.5.1.5
68374lysine decarboxylase-4.1.1.18
68374arginine dihydrolase-3.5.3.6
68374ornithine decarboxylase-4.1.1.17

API ID32E

@refODCADH ArgLDC LysURELARLGAT5KGLIPRPbeta GLUMANMALADOPLEbeta GURMNTINDbeta NAGbeta GALGLUSACLARADARLalpha GLUalpha GALTRERHAINOCELSORalphaMALAspA
47223--------+--------+-+-+----------

Isolation, sampling and environmental information

isolation

@refsample typesampling dategeographic locationcountryorigin.countrycontinent
1925stool of seven month old female with gastroenteritis
472231987
67770Stool
119743Human, 7-month-old female with gastroenteritisHawaiiUnited States of AmericaUSANorth America

isolation source categories

Cat1Cat2Cat3
#Infection#Patient
#Host Body Product#Gastrointestinal tract#Feces (Stool)
#Host#Human#Female

Safety information

risk assessment

@refpathogenicity humanbiosafety levelbiosafety level comment
1925yes, in single cases1Risk group (German classification)
1197431Risk group (French classification)

Sequence information

16S sequences

  • @ref: 20218
  • description: Leminorella grimontii 16S rRNA gene (strain DSM 5078)
  • accession: AJ233421
  • length: 1482
  • database: ena
  • NCBI tax ID: 1005999

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Leminorella grimontii ATCC 33999 = DSM 50781005999.12wgspatric1005999
66792Leminorella grimontii ATCC 33999 = DSM 50781005999.13wgspatric1005999
66792Leminorella grimontii strain NCTC1215282981.4wgspatric82981
66792Leminorella grimontii ATCC 339992588253796draftimg1005999
66792Leminorella grimontii DSM 50782524614760draftimg1005999
67770Leminorella grimontii ATCC 33999 = DSM 5078GCA_000439085scaffoldncbi1005999
67770Leminorella grimontii NCTC12152GCA_900706845contigncbi82981
67770Leminorella grimontii ATCC 33999 = DSM 5078GCA_000735425contigncbi1005999
66792Leminorella grimontii JCM5902GCA_024343555contigncbi82981

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingno100no
motileyes86.247no
flagellatedno64.658no
gram-positiveno98.421no
anaerobicno87.094no
aerobicno59.746no
halophileno93.791no
spore-formingno95.758no
thermophileno99.627yes
glucose-utilyes87.145no
glucose-fermentyes84.807no

External links

@ref: 1925

culture collection no.: DSM 5078, ATCC 33999, CDC 1944-81, CCUG 20908, JCM 5902, CIP 103359, LMG 7912, NCTC 12152

straininfo link

  • @ref: 74477
  • straininfo: 3482

literature

  • topic: Phylogeny
  • Pubmed-ID: 3972991
  • title: Leminorella, a new genus of Enterobacteriaceae: identification of Leminorella grimontii sp. nov. and Leminorella richardii sp. nov. found in clinical specimens.
  • authors: Hickman-Brenner FW, Vohra MP, Huntley-Carter GP, Fanning GR, Lowery VA 3rd, Brenner DJ, Farmer JJ 3rd
  • journal: J Clin Microbiol
  • DOI: 10.1128/jcm.21.2.234-239.1985
  • year: 1985
  • mesh: DNA, Bacterial/analysis, Enterobacteriaceae/*classification/isolation & purification/metabolism, Humans, Nucleic Acid Hybridization, Terminology as Topic
  • topic2: Pathogenicity

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
1925Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 5078)https://www.dsmz.de/collection/catalogue/details/culture/DSM-5078
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
34021Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/15148
47223Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 20908)https://www.ccug.se/strain?id=20908
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
66793Mukherjee et al.10.1038/nbt.3886GEBA: 1,003 reference genomes of bacterial and archaeal isolates expand coverage of the tree of life 35: 676-683 201728604660
66794Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg10.1093/nar/gkaa1025BRENDA, the ELIXIR core data resource in 2021: new developments and updatesNucleic Acids Res. 49: D498-D508 202033211880
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68374Automatically annotated from API ID32E
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch10.21203/rs.3.rs-2527258/v1Predictions based on the model GenomeNet Sporulation v. 1
74477Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID3482.1StrainInfo: A central database for resolving microbial strain identifiers
119743Curators of the CIPCollection of Institut Pasteur (CIP 103359)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20103359