Strain identifier

BacDive ID: 4933

Type strain: Yes

Species: Ewingella americana

Strain Designation: 1468-78

Strain history: CIP <- 1981, PAD Grimont, Inst. Pasteur, Paris, France: strain 1468-78 <- Kentucky State Hlth. Dept., USA

NCBI tax ID(s): 910964 (strain), 41202 (species)

« back to strain detail view
For citation purpose refer to the digital object identifier (doi) of the current version.
Archive
version 8.1 (current version):
version 8:
version 7.1:
version 7:
version 6:
version 5:
version 4.1:
version 4:
version 3:
version 2.1:
version 2:
version 1:
version 8.1 (current version)

General

@ref: 1778

BacDive-ID: 4933

DSM-Number: 4580

keywords: genome sequence, 16S sequence, Bacteria, aerobe, mesophilic, Gram-negative, motile, rod-shaped

description: Ewingella americana 1468-78 is an aerobe, mesophilic, Gram-negative bacterium that was isolated from throat.

NCBI tax id

NCBI tax idMatching level
41202species
910964strain

strain history

@refhistory
1778<- CIP <- P.A.D. Grimont <- CDC, 1468-78
67770CIP 81.94 <-- P. A. D. Grimont; Inst. Pasteur, France; 1468-78 <-- Kentucky State Health Dept., USA.
116326CIP <- 1981, PAD Grimont, Inst. Pasteur, Paris, France: strain 1468-78 <- Kentucky State Hlth. Dept., USA

doi: 10.13145/bacdive4933.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Gammaproteobacteria
  • order: Enterobacterales
  • family: Yersiniaceae
  • genus: Ewingella
  • species: Ewingella americana
  • full scientific name: Ewingella americana Grimont et al. 1984

@ref: 1778

domain: Bacteria

phylum: Proteobacteria

class: Gammaproteobacteria

order: Enterobacteriales, not validated

family: Yersiniaceae

genus: Ewingella

species: Ewingella americana

full scientific name: Ewingella americana Grimont et al. 1984

strain designation: 1468-78

type strain: yes

Morphology

cell morphology

  • @ref: 116326
  • gram stain: negative
  • cell shape: rod-shaped
  • motility: yes

Culture and growth conditions

culture medium

@refnamegrowthcompositionlink
37628MEDIUM 3 - Columbia agaryesColumbia agar (39.000 g);distilled water (1000.000 ml)
1778TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92)yeshttps://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium92.pdf
1778COLUMBIA BLOOD MEDIUM (DSMZ Medium 693)yeshttps://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium693.pdf
116326CIP Medium 3yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=3
116326CIP Medium 72yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=72

culture temp

@refgrowthtypetemperaturerange
1778positivegrowth30mesophilic
37628positivegrowth30mesophilic
46118positivegrowth37mesophilic
67770positivegrowth30mesophilic

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
46118aerobe
116326facultative anaerobe

metabolite utilization

@refmetaboliteutilization activitykind of utilization testedChebi-ID
68371Potassium 5-ketogluconate+builds acid from
68371Potassium 2-ketogluconate+builds acid from
68371L-arabitol-builds acid from18403
68371D-arabitol+builds acid from18333
68371L-fucose-builds acid from18287
68371D-fucose-builds acid from28847
68371D-tagatose-builds acid from16443
68371D-lyxose-builds acid from62318
68371turanose-builds acid from32528
68371gentiobiose+builds acid from28066
68371xylitol-builds acid from17151
68371glycogen-builds acid from28087
68371starch-builds acid from28017
68371raffinose-builds acid from16634
68371melezitose-builds acid from6731
68371inulin-builds acid from15443
68371trehalose+builds acid from27082
68371sucrose-builds acid from17992
68371melibiose-builds acid from28053
68371lactose+builds acid from17716
68371maltose+builds acid from17306
68371cellobiose+builds acid from17057
68371salicin+builds acid from17814
68371esculin+builds acid from4853
68371arbutin+builds acid from18305
68371amygdalin-builds acid from27613
68371N-acetylglucosamine+builds acid from59640
68371methyl alpha-D-glucopyranoside-builds acid from320061
68371methyl alpha-D-mannoside-builds acid from43943
68371D-sorbitol-builds acid from17924
68371D-mannitol+builds acid from16899
68371myo-inositol-builds acid from17268
68371galactitol-builds acid from16813
68371L-rhamnose-builds acid from62345
68371L-sorbose-builds acid from17266
68371D-mannose+builds acid from16024
68371D-fructose+builds acid from15824
68371D-glucose+builds acid from17634
68371D-galactose+builds acid from12936
68371methyl beta-D-xylopyranoside-builds acid from74863
68371ribitol-builds acid from15963
68371L-xylose-builds acid from65328
68371D-xylose-builds acid from65327
68371D-ribose+builds acid from16988
68371L-arabinose-builds acid from30849
68371D-arabinose-builds acid from17108
68371erythritol-builds acid from17113
68371glycerol+builds acid from17754
116326nitrate+reduction17632
116326nitrite-reduction16301

metabolite production

  • @ref: 116326
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

enzymes

@refvalueactivityec
68382alpha-fucosidase-3.2.1.51
68382alpha-mannosidase-3.2.1.24
68382N-acetyl-beta-glucosaminidase+3.2.1.52
68382beta-glucosidase-3.2.1.21
68382alpha-glucosidase-3.2.1.20
68382beta-glucuronidase-3.2.1.31
68382beta-galactosidase-3.2.1.23
68382alpha-galactosidase-3.2.1.22
68382naphthol-AS-BI-phosphohydrolase+
68382acid phosphatase+3.1.3.2
68382alpha-chymotrypsin-3.4.21.1
68382trypsin-3.4.21.4
68382cystine arylamidase-3.4.11.3
68382valine arylamidase-
68382leucine arylamidase+3.4.11.1
68382lipase (C 14)-
68382esterase lipase (C 8)+
68382esterase (C 4)+
68382alkaline phosphatase+3.1.3.1
116326oxidase-
116326catalase+1.11.1.6
116326urease-3.5.1.5

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
116326-+++-+----++-----+--

API 50CHac

@refGLYERYDARALARARIBDXYLLXYLADOMDXGALGLUFRUMNESBERHADULINOMANSORMDMMDGNAGAMYARBESCSALCELMALLACMELSACTREINUMLZRAFAMDGLYGXLTGENTURLYXTAGDFUCLFUCDARLLARLGNT2KG5KG
116326+---+----++++----+---+-++++++--+------+-----+-+/-++

Isolation, sampling and environmental information

isolation

@refsample typecountryorigin.countrycontinentgeographic locationisolation date
1778throat
46118Human throat
67770Human throatUSAUSANorth America
116326Human, ThroatUnited States of AmericaUSANorth AmericaKentucky1978

isolation source categories

  • Cat1: #Host Body-Site
  • Cat2: #Oral cavity and airways
  • Cat3: #Throat

taxonmaps

  • @ref: 69479
  • File name: preview.99_7661.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_6;96_106;97_324;98_5604;99_7661&stattab=map
  • Last taxonomy: Yersiniaceae
  • 16S sequence: JN175329
  • Sequence Identity:
  • Total samples: 805
  • soil counts: 42
  • aquatic counts: 108
  • animal counts: 420
  • plant counts: 235

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
17782Risk group (German classification)
1163261Risk group (French classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Ewingella americana strain CIP 81.94 16S ribosomal RNA gene, complete sequenceJN1753291534ena41202
67770Ewingella americana gene for 16S rRNA, partial sequence, strain: GTC 1277AB2737451489ena41202

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Ewingella americana NCTC12157GCA_900451015contigncbi41202
66792Ewingella americana ATCC 33852910964.7wgspatric910964
66792Ewingella americana strain NCTC1215741202.9wgspatric41202
66792Ewingella americana C.024, ATCC 338522588253786draftimg910964
67770Ewingella americana ATCC 33852GCA_000735345contigncbi910964
67770Ewingella americana CCUG 14506TGCA_008693045contigncbi41202

GC content

  • @ref: 67770
  • GC-content: 53.6-55.2
  • method: thermal denaturation, midpoint method (Tm)

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
motileyes91.035no
flagellatedno60.901no
gram-positiveno98.123no
anaerobicno98.726yes
halophileno89.935no
spore-formingno96.353no
thermophileno99.688yes
glucose-utilyes95.627no
aerobicyes72.937no
glucose-fermentyes91.105no

External links

@ref: 1778

culture collection no.: CCUG 14506, NCTC 12157, LMG 7869, CECT 859, CCM 4555, JCM 5911, DSM 4580, ATCC 33852, CIP 81.94, NRRL B-24919, CDC 1468-78, JCM 32812, GTC 1277, ICMP 15665

straininfo link

  • @ref: 74430
  • straininfo: 13669

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny6847036Ewingella americana gen.nov., sp.nov., a new Enterobacteriaceae isolated from clinical specimens.Grimont PA, Farmer JJ 3rd, Grimont F, Asbury MA, Brenner DJ, Deval CAnn Microbiol (Paris)10.1016/0769-2609(83)90102-31983Base Composition, DNA, Bacterial, Enterobacteriaceae/classification/genetics/*isolation & purification, Genotype, Humans, Nucleic Acid Hybridization, Phenotype, Terminology as TopicGenetics
Pathogenicity14598935Natural antibiotic susceptibility of Ewingella americana strains.Stock I, Sherwood KJ, Wiedemann BJ Chemother10.1179/joc.2003.15.5.4282003Databases, Factual, *Drug Resistance, Bacterial, Enterobacteriaceae/*drug effects/*pathogenicity, Enterobacteriaceae Infections/*drug therapy, Microbial Sensitivity Tests

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
1778Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 4580)https://www.dsmz.de/collection/catalogue/details/culture/DSM-4580
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
37628Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/11261
46118Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 14506)https://www.ccug.se/strain?id=14506
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
66794Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg10.1093/nar/gkaa1025BRENDA, the ELIXIR core data resource in 2021: new developments and updatesNucleic Acids Res. 49: D498-D508 202033211880
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68371Automatically annotated from API 50CH acid
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
74430Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID13669.1StrainInfo: A central database for resolving microbial strain identifiers
116326Curators of the CIPCollection of Institut Pasteur (CIP 81.94)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%2081.94