Strain identifier

BacDive ID: 493

Type strain: Yes

Species: Marinobacter adhaerens

Strain Designation: HP15

Strain history: CIP <- 2010, H.P. Grossart, IGB, Stechlin, Germany <- E Kappel, Jacobs Univ., Bremen, Germany: strain HP15

NCBI tax ID(s): 1033846 (species)

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General

@ref: 17197

BacDive-ID: 493

DSM-Number: 23420

keywords: genome sequence, 16S sequence, Bacteria, aerobe, mesophilic, Gram-negative, motile, rod-shaped, colony-forming

description: Marinobacter adhaerens HP15 is an aerobe, mesophilic, Gram-negative bacterium that forms circular colonies and was isolated from marine aggregates from surface waters.

NCBI tax id

  • NCBI tax id: 1033846
  • Matching level: species

strain history

@refhistory
17197<- Eva Kaeppel, Jacobs University Bremen, Germany; HP15 <- H. P. Grossart, ICMB, Univ. Oldenburg, Germany
116040CIP <- 2010, H.P. Grossart, IGB, Stechlin, Germany <- E Kappel, Jacobs Univ., Bremen, Germany: strain HP15

doi: 10.13145/bacdive493.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Gammaproteobacteria
  • order: Alteromonadales
  • family: Alteromonadaceae
  • genus: Marinobacter
  • species: Marinobacter adhaerens
  • full scientific name: Marinobacter adhaerens Kaeppel et al. 2012

@ref: 17197

domain: Bacteria

phylum: Proteobacteria

class: Gammaproteobacteria

order: Alteromonadales

family: Alteromonadaceae

genus: Marinobacter

species: Marinobacter adhaerens

full scientific name: Marinobacter adhaerens Kaeppel et al. 2012

strain designation: HP15

type strain: yes

Morphology

cell morphology

@refgram staincell lengthcell widthcell shapemotilityflagellum arrangement
23126negative1.7-2.4 µm0.6-0.8 µmrod-shapedyespolar
116040negativerod-shapedyes

colony morphology

  • @ref: 23126
  • colony size: 1.0-2.0 mm
  • colony color: brown
  • colony shape: circular
  • incubation period: 2 days
  • medium used: MB agar

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
17197BACTO MARINE BROTH (DIFCO 2216) (DSMZ Medium 514)yeshttps://mediadive.dsmz.de/medium/514Name: BACTO MARINE BROTH (DIFCO 2216) (DSMZ Medium 514) Composition: NaCl 19.45 g/l MgCl2 5.9 g/l Bacto peptone 5.0 g/l Na2SO4 3.24 g/l CaCl2 1.8 g/l Yeast extract 1.0 g/l KCl 0.55 g/l NaHCO3 0.16 g/l Fe(III) citrate 0.1 g/l KBr 0.08 g/l SrCl2 0.034 g/l H3BO3 0.022 g/l Na2HPO4 0.008 g/l Na-silicate 0.004 g/l NaF 0.0024 g/l (NH4)NO3 0.0016 g/l Distilled water
23126MB agaryes5 g peptone, 1 g yeast extract, 0.1 g FePO 4, 6 g agar in 750 ml North Sea water and 250 ml distilled water
39759Marine agar (MA)yesDistilled water make up to (1000.000 ml);Marine agar (55.100 g)
116040CIP Medium 13yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=13

culture temp

@refgrowthtypetemperaturerange
17197positivegrowth37mesophilic
23126positivemaximum45thermophilic
23126positiveminimum4psychrophilic
23126positiveoptimum34-38mesophilic
39759positivegrowth30mesophilic

culture pH

@refabilitytypepHPH range
23126positiveminimum>5.5
23126positivemaximum<10.5alkaliphile
23126positiveoptimum7.0-9.0

Physiology and metabolism

oxygen tolerance

  • @ref: 23126
  • oxygen tolerance: aerobe

spore formation

  • @ref: 23126
  • spore formation: no

halophily

@refsaltgrowthtested relationconcentration
23126NaClnogrowth0 %(w/v)
23126NaClnogrowth25 %(w/v)
23126NaClpositiveoptimum2-6 %(w/v)
23126NaClpositiveminimum>0.5 %(w/v)
23126NaClpositivemaximum<20 %(w/v)

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
2312617521(-)-quinic acid-carbon source
23126620642,3-butanediol-carbon source
23126182404-hydroxy-L-proline-carbon source
23126181014-hydroxyphenylacetic acid-carbon source
2312617128adipate-carbon source
2312615963ribitol-carbon source
2312636219alpha-lactose-carbon source
2312640585alpha-cyclodextrin-carbon source
23126645522-hydroxybutyrate-carbon source
2312627689decanoate-carbon source
2312617057cellobiose-carbon source
2312616947citrate-carbon source
2312618333D-arabitol-carbon source
2312615824D-fructose-carbon source
2312615895D-galactonic acid lactone-carbon source
2312612936D-galactose-carbon source
2312618024D-galacturonic acid-carbon source
2312630612D-glucarate-carbon source
2312617634D-glucose-carbon source
2312614314D-glucose 6-phosphate-carbon source
2312616899D-mannitol-carbon source
2312616024D-mannose-carbon source
2312616523D-serine-carbon source
2312617924D-sorbitol-carbon source
2312617126DL-carnitine-carbon source
2312617113erythritol-carbon source
2312616000ethanolamine-carbon source
2312615740formate-carbon source
2312616865gamma-aminobutyric acid-carbon source
2312628066gentiobiose-carbon source
2312629042glucose 1-phosphate-carbon source
2312632323glucuronamide-carbon source
2312617754glycerol-carbon source
2312615978glycerol 3-phosphate-carbon source
2312673804glycyl L-aspartic acid-carbon source
2312617596inosine-carbon source
2312617240itaconate-carbon source
2312673786L-alanylglycine-carbon source
2312630849L-arabinose-carbon source
2312629991L-aspartate-carbon source
2312618287L-fucose-carbon source
2312615971L-histidine-carbon source
2312615729L-ornithine-carbon source
2312617295L-phenylalanine-carbon source
2312618183L-pyroglutamic acid-carbon source
2312662345L-rhamnose-carbon source
2312617115L-serine-carbon source
2312616857L-threonine-carbon source
231266359lactulose-carbon source
2312615792malonate-carbon source
2312617306maltose-carbon source
2312628053melibiose-carbon source
2312637657methyl D-glucoside-carbon source
2312617268myo-inositol-carbon source
2312628037N-acetylgalactosamine-carbon source
23126506227N-acetylglucosamine-carbon source
2312673784glycyl-l-glutamate-carbon source
2312650048phenylethylamine-carbon source
2312617272propionate-carbon source
2312633951psicose-carbon source
2312617148putrescine-carbon source
2312616634raffinose-carbon source
2312641865sebacic acid-carbon source
2312617992sucrose-carbon source
2312617748thymidine-carbon source
2312627082trehalose-carbon source
2312632528turanose-carbon source
2312616704uridine-carbon source
2312627248urocanic acid-carbon source
2312617151xylitol-carbon source
2312627897tryptophan-energy source
2312617632nitrate-reduction
23126286442-oxopentanoate+carbon source
23126174265-dehydro-D-gluconate+carbon source
231268295beta-hydroxybutyrate+carbon source
2312673706bromosuccinate+carbon source
2312616383cis-aconitate+carbon source
2312615570D-alanine+carbon source
2312623652dextrin+carbon source
2312624996lactate+carbon source
23126167244-hydroxybutyrate+carbon source
2312621217L-alaninamide+carbon source
2312616977L-alanine+carbon source
2312629985L-glutamate+carbon source
2312615603L-leucine+carbon source
2312617203L-proline+carbon source
2312625115malate+carbon source
2312675146monomethyl succinate+carbon source
2312618401phenylacetate+carbon source
2312615361pyruvate+carbon source
2312653423tween 40+carbon source
2312653426tween 80+carbon source
11604017632nitrate-reduction
11604016301nitrite-reduction

metabolite production

@refChebi-IDmetaboliteproduction
2312635581indoleno
11604035581indoleno

metabolite tests

  • @ref: 23126
  • Chebi-ID: 35581
  • metabolite: indole
  • indole test: -

enzymes

@refvalueactivityec
231266-phospho-beta-galactosidase-3.2.1.85
23126arginine dihydrolase-3.5.3.6
23126beta-glucosidase-3.2.1.21
23126gelatinase-
23126tryptophan deaminase-4.1.99.1
23126urease-3.5.1.5
68382alpha-fucosidase-3.2.1.51
68382alpha-mannosidase-3.2.1.24
68382N-acetyl-beta-glucosaminidase+3.2.1.52
68382beta-glucosidase-3.2.1.21
68382alpha-glucosidase-3.2.1.20
68382beta-glucuronidase-3.2.1.31
68382beta-galactosidase-3.2.1.23
68382alpha-galactosidase-3.2.1.22
68382naphthol-AS-BI-phosphohydrolase+
68382acid phosphatase+3.1.3.2
68382alpha-chymotrypsin-3.4.21.1
68382trypsin-3.4.21.4
68382cystine arylamidase-3.4.11.3
68382valine arylamidase-
68382leucine arylamidase+3.4.11.1
68382lipase (C 14)+
68382esterase lipase (C 8)+
68382esterase (C 4)+
68382alkaline phosphatase+3.1.3.1
116040oxidase+
116040catalase+1.11.1.6
116040urease-3.5.1.5

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
116040-+++++----++-----+--

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinentisolation date
17197marine aggregates (0.1-1 mm in diameter) from surface watersGerman Wadden SeaGermanyDEUEurope
116040Environment, Marine aggregates, surface watersWadden SeaGermanyDEUEurope2001

isolation source categories

Cat1Cat2Cat3
#Environmental#Aquatic#Marine
#Environmental#Terrestrial

taxonmaps

  • @ref: 69479
  • File name: preview.99_2437.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_89;96_1317;97_1564;98_1903;99_2437&stattab=map
  • Last taxonomy: Marinobacter
  • 16S sequence: AY241552
  • Sequence Identity:
  • Total samples: 3438
  • soil counts: 149
  • aquatic counts: 3127
  • animal counts: 133
  • plant counts: 29

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
171971Risk group (German classification)
1160401Risk group (French classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
23126Marinobacter adhaerens strain HP15 16S ribosomal RNA gene, partial sequenceAY241552.1531nuccore225937
17197Marinobacter adhaerens HP15 16S ribosomal RNA gene, partial sequenceAY2415521531ena225937

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Marinobacter adhaerens HP15GCA_000166295completencbi225937
66792Marinobacter adhaerens HP15225937.20plasmidpatric225937
66792Marinobacter adhaerens HP15225937.19plasmidpatric225937
66792Marinobacter adhaerens HP15225937.3completepatric225937
66792Marinobacter adhaerens HP15650377991completeimg225937

GC content

@refGC-contentmethod
1719756.9sequence analysis
2312656.9

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
motileyes90.86yes
flagellatedyes91.533yes
gram-positiveno98.538no
anaerobicno98.01no
aerobicyes85.158yes
halophileyes92.71no
spore-formingno96.03yes
glucose-utilno52.072no
thermophileno99.08no
glucose-fermentno89.011no

External links

@ref: 17197

culture collection no.: DSM 23420, CIP 110141, NCBI AY241552

straininfo link

  • @ref: 70172
  • straininfo: 379044

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny21335492Marinobacter adhaerens sp. nov., isolated from marine aggregates formed with the diatom Thalassiosira weissflogii.Kaeppel EC, Gardes A, Seebah S, Grossart HP, Ullrich MSInt J Syst Evol Microbiol10.1099/ijs.0.030189-02011Bacterial Typing Techniques, Base Composition, Cluster Analysis, DNA, Bacterial/chemistry/genetics, DNA, Ribosomal/chemistry/genetics, Diatoms/*microbiology, Fatty Acids/analysis, Hydrogen-Ion Concentration, Locomotion, Marinobacter/*classification/genetics/*isolation & purification/physiology, Molecular Sequence Data, Nucleic Acid Hybridization, Phylogeny, Quinones/analysis, RNA, Ribosomal, 16S/genetics, Seawater/*microbiology, Sequence Analysis, DNA, Sodium Chloride/metabolism, TemperatureGenetics
Phylogeny35094174Marinobacter arenosus sp. nov., a halotolerant bacterium isolated from a tidal flat.Lee Y, Weerawongwiwat V, Kim JH, Suh MK, Kim HS, Lee JS, Yoon JH, Sukhoom A, Kim WArch Microbiol10.1007/s00203-021-02696-72022Bacterial Typing Techniques, DNA, Bacterial/genetics, *Marinobacter/genetics, Phospholipids/analysis, Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNATranscriptome

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
17197Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 23420)https://www.dsmz.de/collection/catalogue/details/culture/DSM-23420
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
23126Eva C. Kaeppel, Astrid Gärdes, Shalin Seebah, Hans-Peter Grossart, Matthias S. Ullrich10.1099/ijs.0.030189-0Marinobacter adhaerens sp. nov., isolated from marine aggregates formed with the diatom Thalassiosira weissflogiiIJSEM 62: 124-128 201221335492
39759Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/7966
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
70172Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID379044.1StrainInfo: A central database for resolving microbial strain identifiers
116040Curators of the CIPCollection of Institut Pasteur (CIP 110141)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20110141