Strain identifier

BacDive ID: 492

Type strain: Yes

Species: Marinobacter oulmenensis

Strain history: <- M. Monteoliva-Sánchez, Univ. Granada, Campus Cartuja, Fac. Pharmacy, Spain <- K. Kharroub; Set74

NCBI tax ID(s): 643747 (species)

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General

@ref: 16182

BacDive-ID: 492

DSM-Number: 22359

keywords: genome sequence, 16S sequence, Bacteria, aerobe, moderately halophilic, thermophilic, Gram-negative, motile, rod-shaped, colony-forming

description: Marinobacter oulmenensis DSM 22359 is an aerobe, moderately halophilic, thermophilic bacterium that forms circular colonies and was isolated from brine of a salt concentrator .

NCBI tax id

  • NCBI tax id: 643747
  • Matching level: species

strain history

  • @ref: 16182
  • history: <- M. Monteoliva-Sánchez, Univ. Granada, Campus Cartuja, Fac. Pharmacy, Spain <- K. Kharroub; Set74

doi: 10.13145/bacdive492.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Gammaproteobacteria
  • order: Alteromonadales
  • family: Alteromonadaceae
  • genus: Marinobacter
  • species: Marinobacter oulmenensis
  • full scientific name: Marinobacter oulmenensis Kharroub et al. 2011

@ref: 16182

domain: Bacteria

phylum: Proteobacteria

class: Gammaproteobacteria

order: Alteromonadales

family: Alteromonadaceae

genus: Marinobacter

species: Marinobacter oulmenensis

full scientific name: Marinobacter oulmenensis Kharroub et al. 2011

type strain: yes

Morphology

cell morphology

@refgram staincell lengthcell widthcell shapemotilityconfidence
23125negative1.6-3.0 µm0.3-0.5 µmrod-shapedyes
69480yes97.402
69480negative99.996

colony morphology

  • @ref: 23125
  • colony size: 2.0-3.0 mm
  • colony color: cream
  • colony shape: circular
  • incubation period: 3 days
  • medium used: MH agar

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
16182MEDIUM 514 PLUS ADDITIONAL SALT (DSMZ Medium 514b)yeshttps://mediadive.dsmz.de/medium/514bName: MEDIUM 514 plus additional salt (DSMZ Medium 514b) Composition: NaCl 19.45 g/l Agar 17.5 g/l MgCl2 5.9 g/l Bacto peptone 5.0 g/l Na2SO4 3.24 g/l CaCl2 1.8 g/l Yeast extract 1.0 g/l KCl 0.55 g/l NaHCO3 0.16 g/l Fe(III) citrate 0.1 g/l KBr 0.08 g/l SrCl2 0.034 g/l H3BO3 0.022 g/l Na2HPO4 0.008 g/l Na-silicate 0.004 g/l NaF 0.0024 g/l (NH4)NO3 0.0016 g/l Distilled water
23125MHyes7.5% (w/v) NaCl

culture temp

@refgrowthtypetemperaturerange
23125positivemaximum45thermophilic
23125positiveminimum30mesophilic
23125positiveoptimum37-40

culture pH

@refabilitytypepHPH range
23125positiveminimum5.5
23125positivemaximum9.0alkaliphile
23125positiveoptimum6.5-7.0

Physiology and metabolism

oxygen tolerance

  • @ref: 23125
  • oxygen tolerance: aerobe

spore formation

@refspore formationconfidence
23125no
69481no100
69480no99.998

halophily

@refhalophily levelsaltgrowthtested relationconcentration
23125moderately halophilicNaClpositivegrowth>1 %(w/v)
23125NaClpositivegrowth<15 %(w/v)
23125NaClpositiveoptimum5.0-7.5 %(w/v)

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
2312515963ribitol-builds acid from
2312517057cellobiose-builds acid from
2312515824D-fructose-builds acid from
2312512936D-galactose-builds acid from
2312517634D-glucose-builds acid from
2312516024D-mannose-builds acid from
2312565327D-xylose-builds acid from
2312517754glycerol-builds acid from
2312530849L-arabinose-builds acid from
2312516634raffinose-builds acid from
2312517992sucrose-builds acid from
2312527897tryptophan-energy source
2312517234glucose-fermentation
2312515963ribitol-growth
2312516150benzoate-growth
2312517057cellobiose-growth
2312515824D-fructose-growth
2312512936D-galactose-growth
2312517634D-glucose-growth
2312516024D-mannose-growth
2312565327D-xylose-growth
2312524996lactate-growth
2312517754glycerol-growth
2312515428glycine-growth
2312516977L-alanine-growth
2312530849L-arabinose-growth
2312517196L-asparagine-growth
2312517561L-cysteine-growth
2312529985L-glutamate-growth
2312518019L-lysine-growth
2312516643L-methionine-growth
2312516828L-tryptophan-growth
2312517895L-tyrosine-growth
2312518401phenylacetate-growth
2312516634raffinose-growth
2312517992sucrose-growth
2312516991dna-hydrolysis
231254853esculin-hydrolysis
231255291gelatin-hydrolysis
2312528017starch-hydrolysis
2312553426tween 80-hydrolysis
2312517632nitrate-reduction
2312517632nitrate-respiration
2312516899D-mannitol+builds acid from
2312516813galactitol+builds acid from
2312516947citrate+carbon source
2312530089acetate+growth
2312529016arginine+growth
2312515748D-glucuronate+growth
2312516899D-mannitol+growth
2312516813galactitol+growth
2312515740formate+growth
2312529806fumarate+growth
2312517754glycerol+growth
2312525115malate+growth
2312515792malonate+growth
2312530623oxalate+growth
2312530031succinate+growth
2312528938ammonium+nitrogen source

antibiotic resistance

@refChEBImetaboliteis antibioticis resistantresistance conc.is sensitivesensitivity conc.
231253745clindamycinyesyes2 Unit
2312528971ampicillinyesyes10 µg (disc)
2312528368novobiocinyesyes30 µg (disc)
2312518208penicillin gyesyes10 Unit
2312517833gentamicinyesyes10 µg (disc)
2312548923erythromycinyesyes15 µg (disc)
2312517698chloramphenicolyesyes30 µg (disc)
231256104kanamycinyesyes30 µg (disc)

metabolite production

  • @ref: 23125
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

metabolite tests

@refChebi-IDmetaboliteindole testcitrate test
2312535581indole-
2312516947citrate+

enzymes

@refvalueactivityec
231256-phospho-beta-galactosidase+3.2.1.85
23125arginine dihydrolase-3.5.3.6
23125catalase+1.11.1.6
23125cytochrome oxidase+1.9.3.1
23125lysine decarboxylase-4.1.1.18
23125ornithine decarboxylase-4.1.1.17
23125tryptophan deaminase-4.1.99.1
23125urease-3.5.1.5

Isolation, sampling and environmental information

isolation

  • @ref: 16182
  • sample type: brine of a salt concentrator (Sabkha)
  • geographic location: Ain Oulmene (35° 55' 23'' N 5° 17' 36'' E)
  • country: Algeria
  • origin.country: DZA
  • continent: Africa
  • latitude: 35.9231
  • longitude: 5.2933

isolation source categories

Cat1Cat2
#Environmental#Aquatic
#Condition#Saline

taxonmaps

  • @ref: 69479
  • File name: preview.99_10935.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_89;96_693;97_6546;98_8142;99_10935&stattab=map
  • Last taxonomy: Marinobacter oulmenensis subclade
  • 16S sequence: FJ897726
  • Sequence Identity:
  • Total samples: 102
  • soil counts: 6
  • aquatic counts: 94
  • animal counts: 2

Safety information

risk assessment

  • @ref: 16182
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

  • @ref: 16182
  • description: Marinobacter oulmenensis strain Set74 16S ribosomal RNA gene, partial sequence
  • accession: FJ897726
  • length: 1464
  • database: ena
  • NCBI tax ID: 643747

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Marinobacter oulmenensis DSM 22359GCA_014201735scaffoldncbi643747
66792Marinobacter oulmenensis strain DSM 22359643747.3wgspatric643747
66792Marinobacter oulmenensis DSM 223592861259193draftimg643747

GC content

  • @ref: 16182
  • GC-content: 57.4
  • method: thermal denaturation, midpoint method (Tm)

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingno100no
flagellatedyes93.623no
gram-positiveno98.801no
anaerobicno95.566yes
aerobicyes86.772yes
halophileyes88.837yes
spore-formingno96.54yes
glucose-utilno54.734yes
thermophileno99.381no
motileyes93.853yes
glucose-fermentno87.16yes

External links

@ref: 16182

culture collection no.: DSM 22359, CECT 7499, SET Set74, SET 74

straininfo link

  • @ref: 70171
  • straininfo: 378108

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny20935087Marinobacter oulmenensis sp. nov., a moderately halophilic bacterium isolated from brine of a salt concentrator.Kharroub K, Aguilera M, Jimenez-Pranteda ML, Gonzalez-Paredes A, Ramos-Cormenzana A, Monteoliva-Sanchez MInt J Syst Evol Microbiol10.1099/ijs.0.027284-02010Algeria, Bacterial Typing Techniques, Base Composition, Carbon/metabolism, Cluster Analysis, DNA, Bacterial/chemistry/genetics, DNA, Ribosomal/chemistry/genetics, Fatty Acids/analysis, Geologic Sediments/*microbiology, Heterotrophic Processes, Hydrogen-Ion Concentration, Marinobacter/*classification/genetics/*isolation & purification/physiology, Molecular Sequence Data, Nitrogen/metabolism, Nucleic Acid Hybridization, Phospholipids/analysis, Phylogeny, Quinones/analysis, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Sodium Chloride/metabolism, TemperatureMetabolism
Phylogeny28792369Marinobacter aquaticus sp. nov., a moderately halophilic bacterium from a solar saltern.Leon MJ, Sanchez-Porro C, Ventosa AInt J Syst Evol Microbiol10.1099/ijsem.0.0019842017Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Fatty Acids/chemistry, Marinobacter/*classification/genetics/isolation & purification, Nucleic Acid Hybridization, *Phylogeny, Ponds/*microbiology, RNA, Ribosomal, 16S/genetics, *Salinity, Sequence Analysis, DNA, Sodium Chloride, SpainTranscriptome

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
16182Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 22359)https://www.dsmz.de/collection/catalogue/details/culture/DSM-22359
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
23125Karima Kharroub, Margarita Aguilera, María Luján Jiménez-Pranteda, Ana González-Paredes, Alberto Ramos-Cormenzana, Mercedes Monteoliva-Sánchez10.1099/ijs.0.027284-0Marinobacter oulmenensis sp. nov., a moderately halophilic bacterium isolated from brine of a salt concentratorIJSEM 61: 2210-2214 201120935087
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch10.21203/rs.3.rs-2527258/v1Predictions based on the model GenomeNet Sporulation v. 1
70171Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID378108.1StrainInfo: A central database for resolving microbial strain identifiers