Name: Marinobacter oulmenensis DSM 22359
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 492

Type strain:

Species: Marinobacter oulmenensis

Culture col. no.: DSM 22359, CECT 7499, SET Set74, SET 74

Strain history: <- M. Monteoliva-Sánchez, Univ. Granada, Campus Cartuja, Fac. Pharmacy, Spain <- K. Kharroub; Set74

NCBI tax ID(s): 643747 (species)

SI-ID 378108

CECT 7499, DSM 22359

Section

Marinobacter oulmenensis DSM 22359 is an aerobe, moderately halophilic, thermophilic bacterium that forms circular colonies and was isolated from brine of a salt concentrator .

colony-forming
Gram-negative
motile
rod-shaped
aerobe
moderately halophilic
thermophilic
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Pseudomonadota*
	Class	*Gammaproteobacteria*
	Order	*Alteromonadales*
	Family	*Alteromonadaceae*
	Genus	*Marinobacter*
	Species	**Marinobacter oulmenensis**
	Full Scientific Name (LPSN)	Marinobacter oulmenensis Kharroub et al. 2011

Information on morphological and physiological properties Morphology

[Ref.: #23125]	Gram stain	negative
[Ref.: #69480]	Gram stain	negative Confidence in %99.996

[Ref.: #23125]	Cell length	1.6-3.0 µm

[Ref.: #23125]	Cell width	0.3-0.5 µm

[Ref.: #23125]	Cell shape	rod-shaped

[Ref.: #23125]	Motility	yes
[Ref.: #69480]	Motility	yes Confidence in %97.402

[Ref.: #23125]	Colony size	2.0-3.0 mm
[Ref.: #23125]	Colony color	cream
[Ref.: #23125]	Colony shape	circular
[Ref.: #23125]	Incubation period	3 days
[Ref.: #23125]	Cultivation medium used	MH agar

Information on culture and growth conditions Culture and growth conditions

[Ref.: #16182]

Culture medium

MEDIUM 514 PLUS ADDITIONAL SALT (DSMZ Medium 514b)

[Ref.: #16182]

Culture medium growth

[Ref.: #16182]

Culture medium link

Medium recipe at MediaDive

[Ref.: #16182]

Culture medium composition

expand / minimize

[Ref.: #23125]

Culture medium

[Ref.: #23125]

Culture medium growth

[Ref.: #23125]

Culture medium composition

expand / minimize

		Temperature
[Ref.: #23125]	maximum	45 °C
[Ref.: #23125]	minimum	30 °C
[Ref.: #23125]	optimum	37-40 °C

[Ref.: #23125]	Temperature range	thermophilic
[Ref.: #23125]	Temperature range	mesophilic

		pH
[Ref.: #23125]	maximum	9.0
[Ref.: #23125]	minimum	5.5
[Ref.: #23125]	optimum	6.5-7.0

Information on physiology and metabolism Physiology and metabolism

[Ref.: #23125]

Oxygen tolerance

aerobe

[Ref.: #23125]	Ability of spore formation	no
[Ref.: #69481]	Ability of spore formation	no Confidence in %100
[Ref.: #69480]	Ability of spore formation	no Confidence in %99.998

[Ref.: #23125]

Halophily / tolerance level

moderately halophilic

	Salt		Salt conc.
[Ref.: #23125]	NaCl	growth	>1	%(w/v)
[Ref.: #23125]	NaCl	growth	<15	%(w/v)
[Ref.: #23125]	NaCl	optimum	5.0-7.5	%(w/v)

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #23125]	acetate ChEBI	+	growth
[Ref.: #23125]	ammonium ChEBI	+	nitrogen source
[Ref.: #23125]	arginine ChEBI	+	growth
[Ref.: #23125]	benzoate ChEBI	-	growth
[Ref.: #23125]	cellobiose ChEBI	-	builds acid from
[Ref.: #23125]	cellobiose ChEBI	-	growth
[Ref.: #23125]	citrate ChEBI	+	carbon source
[Ref.: #23125]	D-fructose ChEBI	-	builds acid from
[Ref.: #23125]	D-fructose ChEBI	-	growth
[Ref.: #23125]	D-galactose ChEBI	-	builds acid from
[Ref.: #23125]	D-galactose ChEBI	-	growth
[Ref.: #23125]	D-glucose ChEBI	-	builds acid from
[Ref.: #23125]	D-glucose ChEBI	-	growth
[Ref.: #23125]	D-glucuronate ChEBI	+	growth
[Ref.: #23125]	D-mannitol ChEBI	+	builds acid from
[Ref.: #23125]	D-mannitol ChEBI	+	growth
[Ref.: #23125]	D-mannose ChEBI	-	builds acid from
[Ref.: #23125]	D-mannose ChEBI	-	growth
[Ref.: #23125]	D-xylose ChEBI	-	builds acid from
[Ref.: #23125]	D-xylose ChEBI	-	growth
[Ref.: #23125]	dna ChEBI	-	hydrolysis
[Ref.: #23125]	esculin ChEBI	-	hydrolysis
[Ref.: #23125]	formate ChEBI	+	growth
[Ref.: #23125]	fumarate ChEBI	+	growth
[Ref.: #23125]	galactitol ChEBI	+	builds acid from
[Ref.: #23125]	galactitol ChEBI	+	growth
[Ref.: #23125]	gelatin ChEBI	-	hydrolysis
[Ref.: #23125]	glucose ChEBI	-	fermentation
[Ref.: #23125]	glycerol ChEBI	-	builds acid from
[Ref.: #23125]	glycerol ChEBI	-	growth
[Ref.: #23125]	glycerol ChEBI	+	growth
[Ref.: #23125]	glycine ChEBI	-	growth
[Ref.: #23125]	L-alanine ChEBI	-	growth
[Ref.: #23125]	L-arabinose ChEBI	-	builds acid from
[Ref.: #23125]	L-arabinose ChEBI	-	growth
[Ref.: #23125]	L-asparagine ChEBI	-	growth
[Ref.: #23125]	L-cysteine ChEBI	-	growth
[Ref.: #23125]	L-glutamate ChEBI	-	growth
[Ref.: #23125]	L-lysine ChEBI	-	growth
[Ref.: #23125]	L-methionine ChEBI	-	growth
[Ref.: #23125]	L-tryptophan ChEBI	-	growth
[Ref.: #23125]	L-tyrosine ChEBI	-	growth
[Ref.: #23125]	lactate ChEBI	-	growth
[Ref.: #23125]	malate ChEBI	+	growth
[Ref.: #23125]	malonate ChEBI	+	growth
[Ref.: #23125]	nitrate ChEBI	-	reduction
[Ref.: #23125]	nitrate ChEBI	-	respiration
[Ref.: #23125]	oxalate ChEBI	+	growth
[Ref.: #23125]	phenylacetate ChEBI	-	growth
[Ref.: #23125]	raffinose ChEBI	-	builds acid from
[Ref.: #23125]	raffinose ChEBI	-	growth
[Ref.: #23125]	ribitol ChEBI	-	builds acid from
[Ref.: #23125]	ribitol ChEBI	-	growth
[Ref.: #23125]	starch ChEBI	-	hydrolysis
[Ref.: #23125]	succinate ChEBI	+	growth
[Ref.: #23125]	sucrose ChEBI	-	builds acid from
[Ref.: #23125]	sucrose ChEBI	-	growth
[Ref.: #23125]	tryptophan ChEBI	-	energy source
[Ref.: #23125]	tween 80 ChEBI	-	hydrolysis
	Only first 10 entries are displayed. Click here to see all.

	Metabolite	Antibiotic sensitivity	Concentration		Antibiotic resistance	Concentration
[Ref.: #23125]	ampicillin ChEBI				yes	10	µg (disc)
[Ref.: #23125]	chloramphenicol ChEBI	yes	30	µg (disc)
[Ref.: #23125]	clindamycin ChEBI				yes	2	Unit
[Ref.: #23125]	erythromycin ChEBI	yes	15	µg (disc)
[Ref.: #23125]	gentamicin ChEBI	yes	10	µg (disc)
[Ref.: #23125]	kanamycin ChEBI	yes	30	µg (disc)
[Ref.: #23125]	novobiocin ChEBI				yes	30	µg (disc)
[Ref.: #23125]	penicillin g ChEBI	yes	10	Unit

	Metabolite production	Metabolite	Production
[Ref.: #23125]		indole ChEBI	no

	Physiological tests	Metabolite	Indole test	Citrate test
[Ref.: #23125]		citrate ChEBI		+
[Ref.: #23125]		indole ChEBI	-

	Enzyme	Enzyme activity	EC number
[Ref.: #23125]	6-phospho-beta-galactosidase	+	3.2.1.85
[Ref.: #23125]	arginine dihydrolase	-	3.5.3.6
[Ref.: #23125]	catalase	+	1.11.1.6
[Ref.: #23125]	cytochrome oxidase	+	1.9.3.1
[Ref.: #23125]	lysine decarboxylase	-	4.1.1.18
[Ref.: #23125]	ornithine decarboxylase	-	4.1.1.17
[Ref.: #23125]	tryptophan deaminase	-	4.1.99.1
[Ref.: #23125]	urease	-	3.5.1.5

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #16182]	Sample type/isolated from	brine of a salt concentrator (Sabkha)
[Ref.: #16182]	Geographic location (country and/or sea, region)	Ain Oulmene (35° 55' 23'' N 5° 17' 36'' E)
[Ref.: #16182]	Country	Algeria
[Ref.: #16182]	Country ISO 3 Code	DZA
[Ref.: #16182]	Continent	Africa
[Ref.: #16182]	Geographic location	35.9231°/5.2933°
* marker position based on {}

Isolation sources categories

#Environmental	#Aquatic	-
#Condition	#Saline	-

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence FJ897726 (>99% sequence identity) for Marinobacter oulmenensis subclade from Microbeatlas

Aquatic Samples	94
Soil Samples	6
Animal Samples	2
Plant Samples
Total Samples	102

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #16182]

Biosafety level

Risk group (German classification)
According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #16182]	Marinobacter oulmenensis strain Set74 16S ribosomal RNA gene, partial sequence	FJ897726	1464	ENA	643747 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Marinobacter oulmenensis DSM 22359	GCA_014201735	scaffold	GenBank	643747 tax ID	*
[Ref.: #66792]	Marinobacter oulmenensis strain DSM 22359	643747.3	wgs	PATRIC	643747 tax ID	*
[Ref.: #66792]	Marinobacter oulmenensis DSM 22359	2861259193	draft	JGI IMG/M	643747 tax ID	*

[Ref.: #16182]

GC-content

57.4 mol%

thermal denaturation, midpoint method (Tm)

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Marinobacter oulmenensis DSM 22359 NCBI: GCA_014201735.1
[Ref.: #69481]	spore-forming	no	100	no

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Marinobacter oulmenensis DSM 22359 NCBI: GCA_014201735.1
[Ref.: #69480]	flagellated	yes	93.623	no
[Ref.: #69480]	gram-positive	no	98.801	no
[Ref.: #69480]	anaerobic	no	95.566	yes
[Ref.: #69480]	aerobic	yes	86.772	yes
[Ref.: #69480]	halophile	yes	88.837	yes
[Ref.: #69480]	spore-forming	no	96.54	yes
[Ref.: #69480]	glucose-util	no	54.734	yes
[Ref.: #69480]	thermophile	no	99.381	no
[Ref.: #69480]	motile	yes	93.853	yes
[Ref.: #69480]	glucose-ferment	no	87.16	yes

Availability in culture collections External links

[Ref.: #16182]

Culture collection no.

DSM 22359, CECT 7499, SET Set74, SET 74

[Ref.: #70171]

SI-ID 378108

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Marinobacter aquaticus sp. nov., a moderately halophilic bacterium from a solar saltern.	Leon MJ, Sanchez-Porro C, Ventosa A	Int J Syst Evol Microbiol	10.1099/ijsem.0.001984	2017	*
Phylogeny	Marinobacter oulmenensis sp. nov., a moderately halophilic bacterium isolated from brine of a salt concentrator.	Kharroub K, Aguilera M, Jimenez-Pranteda ML, Gonzalez-Paredes A, Ramos-Cormenzana A, Monteoliva-Sanchez M	Int J Syst Evol Microbiol	10.1099/ijs.0.027284-0	2010	*

References

#16182

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 22359

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#23125

Karima Kharroub, Margarita Aguilera, María Luján Jiménez-Pranteda, Ana González-Paredes, Alberto Ramos-Cormenzana, Mercedes Monteoliva-Sánchez: Marinobacter oulmenensis sp. nov., a moderately halophilic bacterium isolated from brine of a salt concentrator. IJSEM 61: 2210 - 2214 2011 ( DOI 10.1099/ijs.0.027284-0 , PubMed 20935087 )

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69481

Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch: Predictions based on the model GenomeNet Sporulation v. 1 . ( DOI 10.21203/rs.3.rs-2527258/v1 )

#70171

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID378108.1 )

* These data were automatically processed and therefore are not curated

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