Strain identifier

BacDive ID: 478

Type strain: Yes

Species: Marinobacter lipolyticus

Strain Designation: SM19

Strain history: CIP <- 2002, A. Ventosa, Sevilla Univ., Sevilla, Spain: strain SM19

NCBI tax ID(s): 209639 (species)

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General

@ref: 5732

BacDive-ID: 478

DSM-Number: 15157

keywords: genome sequence, 16S sequence, Bacteria, facultative aerobe, mesophilic, Gram-negative, motile, rod-shaped, colony-forming

description: Marinobacter lipolyticus SM19 is a facultative aerobe, mesophilic, Gram-negative bacterium that forms circular colonies and was isolated from saline soil.

NCBI tax id

  • NCBI tax id: 209639
  • Matching level: species

strain history

@refhistory
5732<- A. Ventosa; SM19 <- S. Martin
361642002, A. Ventosa, Sevilla Univ., Sevilla, Spain: strain SM19
120621CIP <- 2002, A. Ventosa, Sevilla Univ., Sevilla, Spain: strain SM19

doi: 10.13145/bacdive478.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Gammaproteobacteria
  • order: Alteromonadales
  • family: Alteromonadaceae
  • genus: Marinobacter
  • species: Marinobacter lipolyticus
  • full scientific name: Marinobacter lipolyticus Martín et al. 2003

@ref: 5732

domain: Bacteria

phylum: Proteobacteria

class: Gammaproteobacteria

order: Alteromonadales

family: Alteromonadaceae

genus: Marinobacter

species: Marinobacter lipolyticus

full scientific name: Marinobacter lipolyticus Martín et al. 2003

strain designation: SM19

type strain: yes

Morphology

cell morphology

@refgram staincell lengthcell widthcell shapemotility
23296negative2.5-3.5 µm0.3-0.5 µmrod-shapedyes
120621negativerod-shapedno

colony morphology

  • @ref: 23296
  • colony size: 2-3 mm
  • colony color: cream
  • colony shape: circular
  • medium used: SW 7.5

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
573215% MH MEDIUM (DSMZ Medium 582)yeshttps://mediadive.dsmz.de/medium/582Name: 15% MH MEDIUM (DSMZ Medium 582) Composition: NaCl 121.5 g/l MgSO4 14.4 g/l MgCl2 10.5 g/l Yeast extract 10.0 g/l Proteose peptone no. 3 5.0 g/l KCl 3.0 g/l Glucose 1.0 g/l CaCl2 0.54 g/l NaHCO3 0.09 g/l NaBr 0.039 g/l Distilled water
23296SW 7.5yes
36164Marine agar (MA)yesDistilled water make up to (1000.000 ml);Marine agar (55.100 g)
120621CIP Medium 13yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=13

culture temp

@refgrowthtypetemperaturerange
5732positivegrowth37mesophilic
23296positivegrowth15-40
23296positiveoptimum37mesophilic
36164positivegrowth37mesophilic
120621positivegrowth15-41
120621nogrowth5psychrophilic

culture pH

@refabilitytypepHPH range
23296positivegrowth5.0-10.0alkaliphile
23296positiveoptimum7.5

Physiology and metabolism

oxygen tolerance

  • @ref: 23296
  • oxygen tolerance: facultative aerobe

spore formation

  • @ref: 23296
  • spore formation: no

halophily

@refsaltgrowthtested relationconcentrationhalophily level
23296NaClpositivegrowth1-15 %
23296NaClpositiveoptimum7.5 %
23296NaClnogrowth0 %
23296moderately halophilic
120621NaClpositivegrowth4-8 %
120621NaClnogrowth0 %
120621NaClnogrowth2 %
120621NaClnogrowth10 %

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
2329617716lactose-builds acid from
2329628053melibiose-builds acid from
2329617754glycerol-builds acid from
2329617521(-)-quinic acid-carbon source
23296167632-oxobutanoate-carbon source
23296309162-oxoglutarate-carbon source
23296286442-oxopentanoate-carbon source
23296620642,3-butanediol-carbon source
23296182404-hydroxy-L-proline-carbon source
23296181014-hydroxyphenylacetic acid-carbon source
2329630089acetate-carbon source
2329615963ribitol-carbon source
2329640585alpha-cyclodextrin-carbon source
23296645522-hydroxybutyrate-carbon source
232968295beta-hydroxybutyrate-carbon source
2329673706bromosuccinate-carbon source
2329617057cellobiose-carbon source
2329616383cis-aconitate-carbon source
2329616947citrate-carbon source
2329615570D-alanine-carbon source
2329618333D-arabitol-carbon source
2329612931D-galactonate-carbon source
2329612936D-galactose-carbon source
2329630612D-glucarate-carbon source
2329617784D-glucosaminic acid-carbon source
2329614314D-glucose 6-phosphate-carbon source
2329615748D-glucuronate-carbon source
2329617716lactose-carbon source
2329616024D-mannose-carbon source
2329628053melibiose-carbon source
2329627605D-psicose-carbon source
2329616634raffinose-carbon source
2329616523D-serine-carbon source
2329617924D-sorbitol-carbon source
2329617126DL-carnitine-carbon source
2329617113erythritol-carbon source
2329616000ethanolamine-carbon source
2329615740formate-carbon source
2329616865gamma-aminobutyric acid-carbon source
23296167244-hydroxybutyrate-carbon source
2329629042glucose 1-phosphate-carbon source
2329632323glucuronamide-carbon source
2329617754glycerol-carbon source
2329673804glycyl L-aspartic acid-carbon source
2329617240itaconate-carbon source
2329621217L-alaninamide-carbon source
2329616977L-alanine-carbon source
2329673786L-alanylglycine-carbon source
2329630849L-arabinose-carbon source
2329617196L-asparagine-carbon source
2329629991L-aspartate-carbon source
2329618287L-fucose-carbon source
2329629985L-glutamate-carbon source
2329615971L-histidine-carbon source
2329615603L-leucine-carbon source
2329615729L-ornithine-carbon source
2329617295L-phenylalanine-carbon source
2329617203L-proline-carbon source
2329618183L-pyroglutamic acid-carbon source
2329662345L-rhamnose-carbon source
2329617115L-serine-carbon source
2329616857L-threonine-carbon source
2329624996lactate-carbon source
232966359lactulose-carbon source
2329615792malonate-carbon source
23296320055methyl beta-D-glucopyranoside-carbon source
2329675146monomethyl succinate-carbon source
2329617268myo-inositol-carbon source
2329628037N-acetylgalactosamine-carbon source
2329673784glycyl-l-glutamate-carbon source
2329650048phenylethylamine-carbon source
2329617272propionate-carbon source
2329617148putrescine-carbon source
2329641865sebacic acid-carbon source
23296143136succinamate-carbon source
2329630031succinate-carbon source
2329617992sucrose-carbon source
2329632528turanose-carbon source
2329616704uridine-carbon source
2329627248urocanic acid-carbon source
2329617151xylitol-carbon source
2329627897tryptophan-energy source
2329617234glucose-fermentation
2329616991dna-hydrolysis
232965291gelatin-hydrolysis
2329628017starch-hydrolysis
2329617632nitrate-reduction
2329616301nitrite-reduction
2329617634D-glucose+builds acid from
2329616899D-mannitol+builds acid from
2329617306maltose+builds acid from
2329616947citrate+carbon source
2329615824D-fructose+carbon source
232968391D-gluconate+carbon source
2329617634D-glucose+carbon source
2329616899D-mannitol+carbon source
2329616551D-trehalose+carbon source
2329623652dextrin+carbon source
2329628087glycogen+carbon source
2329617596inosine+carbon source
2329617306maltose+carbon source
2329651850methyl pyruvate+carbon source
23296506227N-acetylglucosamine+carbon source
2329617748thymidine+carbon source
2329653423tween 40+carbon source
2329653426tween 80+carbon source
2329615824D-fructose+energy source
232968391D-gluconate+energy source
2329617634D-glucose+energy source
2329616899D-mannitol+energy source
2329616551D-trehalose+energy source
2329623652dextrin+energy source
2329628087glycogen+energy source
2329617596inosine+energy source
2329617306maltose+energy source
2329651850methyl pyruvate+energy source
23296506227N-acetylglucosamine+energy source
2329617748thymidine+energy source
2329653423tween 40+energy source
2329653426tween 80+energy source
2329653426tween 80+hydrolysis
2329628938ammonium+nitrogen source
1206214853esculin-hydrolysis
12062117632nitrate-builds gas from
12062117632nitrate-reduction
12062116301nitrite-builds gas from
12062116301nitrite-reduction

metabolite production

@refChebi-IDmetaboliteproduction
2329635581indoleno
2329615688acetoinno
12062135581indoleno

metabolite tests

@refChebi-IDmetabolitevoges-proskauer-testmethylred-testindole testcitrate test
2329615688acetoin-
2329617234glucose-
2329635581indole-
2329616947citrate+

enzymes

@refvalueactivityec
23296arginine dihydrolase-3.5.3.6
23296catalase+1.11.1.6
23296cytochrome oxidase+1.9.3.1
23296lysine decarboxylase-4.1.1.18
23296ornithine decarboxylase-4.1.1.17
23296phenylalanine deaminase-4.3.1.5
23296phosphatase-
23296tryptophan deaminase-4.1.99.1
68382alpha-fucosidase-3.2.1.51
68382alpha-mannosidase-3.2.1.24
68382N-acetyl-beta-glucosaminidase+3.2.1.52
68382beta-glucosidase-3.2.1.21
68382alpha-glucosidase+3.2.1.20
68382beta-glucuronidase-3.2.1.31
68382beta-galactosidase-3.2.1.23
68382alpha-galactosidase-3.2.1.22
68382naphthol-AS-BI-phosphohydrolase+
68382acid phosphatase+3.1.3.2
68382alpha-chymotrypsin-3.4.21.1
68382trypsin-3.4.21.4
68382cystine arylamidase-3.4.11.3
68382valine arylamidase-
68382leucine arylamidase+3.4.11.1
68382lipase (C 14)-
68382esterase lipase (C 8)+
68382esterase (C 4)+
68382alkaline phosphatase+3.1.3.1
120621oxidase+
120621beta-galactosidase-3.2.1.23
120621alcohol dehydrogenase-1.1.1.1
120621gelatinase-
120621amylase+
120621caseinase-3.4.21.50
120621catalase+1.11.1.6
120621tween esterase+
120621gamma-glutamyltransferase+2.3.2.2
120621lecithinase+
120621lipase+
120621lysine decarboxylase-4.1.1.18
120621ornithine decarboxylase-4.1.1.17
120621phenylalanine ammonia-lyase-4.3.1.24
120621tryptophan deaminase-
120621urease-3.5.1.5

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
120621-+++-+----++---+-+--

API biotype100

@refGLUFRUGALTREMNESBEMELSACRAFMTEMALLACLTEMbGaMaGaCELGENMbGuESCRIBARAXYLPLERHAFUCMLZDARLLARLXLTDULTAGGLYINOMANMTLTURSORADOHBGLYXERYMDG3MDGSATMUCLTATDTATMTATDMLTLMLTCATETATETTECITGRTGAT2KG5KGTRYNAGGNTPACPATpOBEQATGTEmOBEBATPPATCMTTGEBETPCEABTHINLATCAPCYTHISSUCFUMGREGYTAVTETNTTNGLNITA3OBUAPTGTTPRODALALALASERMNTPROPTYR2KT
120621-------------------------------------------------------------------------------++---------------+--

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinent
5732saline soilSouth SpainSpainESPEurope
120621Environment, Saline soilSouthSpainESPEurope

isolation source categories

Cat1Cat2Cat3
#Environmental#Terrestrial#Soil
#Condition#Saline

taxonmaps

  • @ref: 69479
  • File name: preview.99_8156.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_89;96_1317;97_1564;98_5513;99_8156&stattab=map
  • Last taxonomy: Marinobacter
  • 16S sequence: AY147906
  • Sequence Identity:
  • Total samples: 453
  • soil counts: 114
  • aquatic counts: 312
  • animal counts: 16
  • plant counts: 11

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
57321Risk group (German classification)
1206211Risk group (French classification)

Sequence information

16S sequences

  • @ref: 5732
  • description: Marinobacter lipolyticus 16S ribosomal RNA gene, partial sequence
  • accession: AY147906
  • length: 1473
  • database: ena
  • NCBI tax ID: 209639

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Marinobacter lipolyticus SM19GCA_000397065scaffoldncbi1318628
66792Marinobacter lipolyticus SM191318628.3wgspatric1318628
66792Marinobacter lipolyticus SM192571042755draftimg1318628

GC content

@refGC-contentmethod
573257thermal denaturation, midpoint method (Tm)
2329657.0thermal denaturation, midpoint method (Tm)

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
motileyes91.884yes
motileyes91.884yes
flagellatedyes87.898no
flagellatedyes87.898no
gram-positiveno98.992no
gram-positiveno98.992no
anaerobicno97.088yes
anaerobicno97.088yes
aerobicyes81.395no
aerobicyes81.395no
halophileyes88.64yes
halophileyes88.64yes
spore-formingno96.291yes
spore-formingno96.291yes
thermophileno98.877yes
thermophileno98.877yes
glucose-utilyes60.708yes
glucose-utilyes60.708yes
glucose-fermentno87.18yes
glucose-fermentno87.18yes

External links

@ref: 5732

culture collection no.: DSM 15157, CCM 7048, CIP 107627, NCIMB 13907

straininfo link

  • @ref: 70157
  • straininfo: 127997

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny13130022Marinobacter lipolyticus sp. nov., a novel moderate halophile with lipolytic activity.Martin S, Marquez MC, Sanchez-Porro C, Mellado E, Arahal DR, Ventosa AInt J Syst Evol Microbiol10.1099/ijs.0.02528-02003Alteromonadaceae/classification/genetics/*isolation & purification/metabolism, Base Composition, DNA, Bacterial/chemistry/genetics, DNA, Ribosomal/genetics, Lipolysis, Molecular Sequence Data, Phenotype, Phylogeny, RNA, Bacterial/genetics, RNA, Ribosomal, 16S/genetics, Sodium Chloride, Soil Microbiology, SpainGenetics
Phylogeny26518711Marinobacter aromaticivorans sp. nov., a polycyclic aromatic hydrocarbon-degrading bacterium isolated from sea sediment.Cui Z, Gao W, Xu G, Luan X, Li Q, Yin X, Huang D, Zheng LInt J Syst Evol Microbiol10.1099/ijsem.0.0007222015Base Composition, China, DNA, Bacterial/genetics, Fatty Acids/chemistry, Geologic Sediments/microbiology, Marinobacter/*classification/genetics/isolation & purification, Molecular Sequence Data, Nucleic Acid Hybridization, Phospholipids/chemistry, *Phylogeny, Polycyclic Aromatic Hydrocarbons/*metabolism, RNA, Ribosomal, 16S/genetics, Seawater/*microbiology, Sequence Analysis, DNA, Vitamin K 2/analogs & derivatives/chemistryGenetics

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
5732Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 15157)https://www.dsmz.de/collection/catalogue/details/culture/DSM-15157
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
23296S. Martín, M. C. Márquez, C. Sánchez-Porro, E. Mellado, D. R. Arahal, A. Ventosa10.1099/ijs.0.02528-0Marinobacter lipolyticus sp. nov., a novel moderate halophile with lipolytic activityIJSEM 53: 1383-1387 200313130022
36164Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/5084
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
70157Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID127997.1StrainInfo: A central database for resolving microbial strain identifiers
120621Curators of the CIPCollection of Institut Pasteur (CIP 107627)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20107627