Strain identifier

BacDive ID: 478

Type strain: Yes

Species: Marinobacter lipolyticus

Strain Designation: SM19

Strain history: CIP <- 2002, A. Ventosa, Sevilla Univ., Sevilla, Spain: strain SM19

NCBI tax ID(s): 209639 (species)

« back to strain detail view
For citation purpose refer to the digital object identifier (doi) of the current version.
Archive
version 9.1 (current version):
version 9:
version 8.1:
version 8:
version 7.1:
version 7:
version 6:
version 5:
version 4.1:
version 4:
version 3:
version 2.1:
version 2:
version 1:
version 9.1 (current version)

General

@ref: 5732

BacDive-ID: 478

DSM-Number: 15157

keywords: genome sequence, 16S sequence, Bacteria, facultative aerobe, Gram-negative, motile, rod-shaped, colony-forming

description: Marinobacter lipolyticus SM19 is a facultative aerobe, Gram-negative, motile bacterium that forms circular colonies and was isolated from saline soil.

NCBI tax id

  • NCBI tax id: 209639
  • Matching level: species

strain history

@refhistory
5732<- A. Ventosa; SM19 <- S. Martin
361642002, A. Ventosa, Sevilla Univ., Sevilla, Spain: strain SM19
120621CIP <- 2002, A. Ventosa, Sevilla Univ., Sevilla, Spain: strain SM19

doi: 10.13145/bacdive478.20240916.9.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Gammaproteobacteria
  • order: Alteromonadales
  • family: Alteromonadaceae
  • genus: Marinobacter
  • species: Marinobacter lipolyticus
  • full scientific name: Marinobacter lipolyticus Martín et al. 2003

@ref: 5732

domain: Bacteria

phylum: Proteobacteria

class: Gammaproteobacteria

order: Alteromonadales

family: Alteromonadaceae

genus: Marinobacter

species: Marinobacter lipolyticus

full scientific name: Marinobacter lipolyticus Martín et al. 2003

strain designation: SM19

type strain: yes

Morphology

cell morphology

@refgram staincell lengthcell widthcell shapemotilityconfidence
23296negative2.5-3.5 µm0.3-0.5 µmrod-shapedyes
120621negativerod-shapedno
69480negative98.5

colony morphology

  • @ref: 23296
  • colony size: 2-3 mm
  • colony color: cream
  • colony shape: circular
  • medium used: SW 7.5

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
573215% MH MEDIUM (DSMZ Medium 582)yeshttps://mediadive.dsmz.de/medium/582Name: 15% MH MEDIUM (DSMZ Medium 582) Composition: NaCl 121.5 g/l MgSO4 14.4 g/l MgCl2 10.5 g/l Yeast extract 10.0 g/l Proteose peptone no. 3 5.0 g/l KCl 3.0 g/l Glucose 1.0 g/l CaCl2 0.54 g/l NaHCO3 0.09 g/l NaBr 0.039 g/l Distilled water
23296SW 7.5yes
36164Marine agar (MA)yesDistilled water make up to (1000.000 ml);Marine agar (55.100 g)
120621CIP Medium 13yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=13

culture temp

@refgrowthtypetemperature
5732positivegrowth37
23296positivegrowth15-40
23296positiveoptimum37
36164positivegrowth37
120621positivegrowth15-41
120621nogrowth5

culture pH

@refabilitytypepHPH range
23296positivegrowth5.0-10.0alkaliphile
23296positiveoptimum7.5

Physiology and metabolism

oxygen tolerance

  • @ref: 23296
  • oxygen tolerance: facultative aerobe

spore formation

@refspore formationconfidence
23296no
69480no91.311

halophily

@refsaltgrowthtested relationconcentrationhalophily level
23296NaClpositivegrowth1-15 %
23296NaClpositiveoptimum7.5 %
23296NaClnogrowth0 %
23296moderately halophilic
120621NaClpositivegrowth4-8 %
120621NaClnogrowth0 %
120621NaClnogrowth2 %
120621NaClnogrowth10 %

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
2329617716lactose-builds acid from
2329628053melibiose-builds acid from
2329617754glycerol-builds acid from
2329617521(-)-quinic acid-carbon source
23296167632-oxobutanoate-carbon source
23296309162-oxoglutarate-carbon source
23296286442-oxopentanoate-carbon source
23296620642,3-butanediol-carbon source
23296182404-hydroxy-L-proline-carbon source
23296181014-hydroxyphenylacetic acid-carbon source
2329630089acetate-carbon source
2329615963ribitol-carbon source
2329640585alpha-cyclodextrin-carbon source
23296645522-hydroxybutyrate-carbon source
232968295beta-hydroxybutyrate-carbon source
2329673706bromosuccinate-carbon source
2329617057cellobiose-carbon source
2329616383cis-aconitate-carbon source
2329616947citrate-carbon source
2329615570D-alanine-carbon source
2329618333D-arabitol-carbon source
2329612931D-galactonate-carbon source
2329612936D-galactose-carbon source
2329630612D-glucarate-carbon source
2329617784D-glucosaminic acid-carbon source
2329614314D-glucose 6-phosphate-carbon source
2329615748D-glucuronate-carbon source
2329617716lactose-carbon source
2329616024D-mannose-carbon source
2329628053melibiose-carbon source
2329627605D-psicose-carbon source
2329616634raffinose-carbon source
2329616523D-serine-carbon source
2329617924D-sorbitol-carbon source
2329617126DL-carnitine-carbon source
2329617113erythritol-carbon source
2329616000ethanolamine-carbon source
2329615740formate-carbon source
2329616865gamma-aminobutyric acid-carbon source
23296167244-hydroxybutyrate-carbon source
2329629042glucose 1-phosphate-carbon source
2329632323glucuronamide-carbon source
2329617754glycerol-carbon source
2329673804glycyl L-aspartic acid-carbon source
2329617240itaconate-carbon source
2329621217L-alaninamide-carbon source
2329616977L-alanine-carbon source
2329673786L-alanylglycine-carbon source
2329630849L-arabinose-carbon source
2329617196L-asparagine-carbon source
2329629991L-aspartate-carbon source
2329618287L-fucose-carbon source
2329629985L-glutamate-carbon source
2329615971L-histidine-carbon source
2329615603L-leucine-carbon source
2329615729L-ornithine-carbon source
2329617295L-phenylalanine-carbon source
2329617203L-proline-carbon source
2329618183L-pyroglutamic acid-carbon source
2329662345L-rhamnose-carbon source
2329617115L-serine-carbon source
2329616857L-threonine-carbon source
2329624996lactate-carbon source
232966359lactulose-carbon source
2329615792malonate-carbon source
23296320055methyl beta-D-glucopyranoside-carbon source
2329675146monomethyl succinate-carbon source
2329617268myo-inositol-carbon source
2329628037N-acetylgalactosamine-carbon source
2329673784glycyl-l-glutamate-carbon source
2329650048phenylethylamine-carbon source
2329617272propionate-carbon source
2329617148putrescine-carbon source
2329641865sebacic acid-carbon source
23296143136succinamate-carbon source
2329630031succinate-carbon source
2329617992sucrose-carbon source
2329632528turanose-carbon source
2329616704uridine-carbon source
2329627248urocanic acid-carbon source
2329617151xylitol-carbon source
2329627897tryptophan-energy source
2329617234glucose-fermentation
2329616991dna-hydrolysis
232965291gelatin-hydrolysis
2329628017starch-hydrolysis
2329617632nitrate-reduction
2329616301nitrite-reduction
2329617634D-glucose+builds acid from
2329616899D-mannitol+builds acid from
2329617306maltose+builds acid from
2329616947citrate+carbon source
2329615824D-fructose+carbon source
232968391D-gluconate+carbon source
2329617634D-glucose+carbon source
2329616899D-mannitol+carbon source
2329616551D-trehalose+carbon source
2329623652dextrin+carbon source
2329628087glycogen+carbon source
2329617596inosine+carbon source
2329617306maltose+carbon source
2329651850methyl pyruvate+carbon source
23296506227N-acetylglucosamine+carbon source
2329617748thymidine+carbon source
2329653423tween 40+carbon source
2329653426tween 80+carbon source
2329615824D-fructose+energy source
232968391D-gluconate+energy source
2329617634D-glucose+energy source
2329616899D-mannitol+energy source
2329616551D-trehalose+energy source
2329623652dextrin+energy source
2329628087glycogen+energy source
2329617596inosine+energy source
2329617306maltose+energy source
2329651850methyl pyruvate+energy source
23296506227N-acetylglucosamine+energy source
2329617748thymidine+energy source
2329653423tween 40+energy source
2329653426tween 80+energy source
2329653426tween 80+hydrolysis
2329628938ammonium+nitrogen source
1206214853esculin-hydrolysis
12062117632nitrate-builds gas from
12062117632nitrate-reduction
12062116301nitrite-builds gas from
12062116301nitrite-reduction

metabolite production

@refChebi-IDmetaboliteproduction
2329635581indoleno
2329615688acetoinno
12062135581indoleno

metabolite tests

@refChebi-IDmetabolitevoges-proskauer-testmethylred-testindole testcitrate test
2329615688acetoin-
2329617234glucose-
2329635581indole-
2329616947citrate+

enzymes

@refvalueactivityec
23296arginine dihydrolase-3.5.3.6
23296catalase+1.11.1.6
23296cytochrome oxidase+1.9.3.1
23296lysine decarboxylase-4.1.1.18
23296ornithine decarboxylase-4.1.1.17
23296phenylalanine deaminase-4.3.1.5
23296phosphatase-
23296tryptophan deaminase-4.1.99.1
68382alpha-fucosidase-3.2.1.51
68382alpha-mannosidase-3.2.1.24
68382N-acetyl-beta-glucosaminidase+3.2.1.52
68382beta-glucosidase-3.2.1.21
68382alpha-glucosidase+3.2.1.20
68382beta-glucuronidase-3.2.1.31
68382beta-galactosidase-3.2.1.23
68382alpha-galactosidase-3.2.1.22
68382naphthol-AS-BI-phosphohydrolase+
68382acid phosphatase+3.1.3.2
68382alpha-chymotrypsin-3.4.21.1
68382trypsin-3.4.21.4
68382cystine arylamidase-3.4.11.3
68382valine arylamidase-
68382leucine arylamidase+3.4.11.1
68382lipase (C 14)-
68382esterase lipase (C 8)+
68382esterase (C 4)+
68382alkaline phosphatase+3.1.3.1
120621oxidase+
120621beta-galactosidase-3.2.1.23
120621alcohol dehydrogenase-1.1.1.1
120621gelatinase-
120621amylase+
120621caseinase-3.4.21.50
120621catalase+1.11.1.6
120621tween esterase+
120621gamma-glutamyltransferase+2.3.2.2
120621lecithinase+
120621lipase+
120621lysine decarboxylase-4.1.1.18
120621ornithine decarboxylase-4.1.1.17
120621phenylalanine ammonia-lyase-4.3.1.24
120621tryptophan deaminase-
120621urease-3.5.1.5

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
120621-+++-+----++---+-+--

API biotype100

@refGLUFRUGALTREMNESBEMELSACRAFMTEMALLACLTEMbGaMaGaCELGENMbGuESCRIBARAXYLPLERHAFUCMLZDARLLARLXLTDULTAGGLYINOMANMTLTURSORADOHBGLYXERYMDG3MDGSATMUCLTATDTATMTATDMLTLMLTCATETATETTECITGRTGAT2KG5KGTRYNAGGNTPACPATpOBEQATGTEmOBEBATPPATCMTTGEBETPCEABTHINLATCAPCYTHISSUCFUMGREGYTAVTETNTTNGLNITA3OBUAPTGTTPRODALALALASERMNTPROPTYR2KT
120621-------------------------------------------------------------------------------++---------------+--

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinent
5732saline soilSouth SpainSpainESPEurope
120621Environment, Saline soilSouthSpainESPEurope

isolation source categories

Cat1Cat2Cat3
#Environmental#Terrestrial#Soil
#Condition#Saline

taxonmaps

  • @ref: 69479
  • File name: preview.99_8156.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_89;96_1317;97_1564;98_5513;99_8156&stattab=map
  • Last taxonomy: Marinobacter
  • 16S sequence: AY147906
  • Sequence Identity:
  • Total samples: 453
  • soil counts: 114
  • aquatic counts: 312
  • animal counts: 16
  • plant counts: 11

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
57321Risk group (German classification)
1206211Risk group (French classification)

Sequence information

16S sequences

  • @ref: 5732
  • description: Marinobacter lipolyticus 16S ribosomal RNA gene, partial sequence
  • accession: AY147906
  • length: 1473
  • database: nuccore
  • NCBI tax ID: 209639

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Marinobacter lipolyticus SM19GCA_000397065scaffoldncbi1318628
66792Marinobacter lipolyticus SM191318628.3wgspatric1318628
66792Marinobacter lipolyticus SM192571042755draftimg1318628

GC content

@refGC-contentmethod
573257thermal denaturation, midpoint method (Tm)
2329657.0thermal denaturation, midpoint method (Tm)

Genome-based predictions

predictions

@reftraitmodeldescriptionpredictionconfidencetraining_data
69480gram-positivegram-positivePositive reaction to Gram-stainingno98.5no
69480anaerobicanaerobicAbility to grow under anoxygenic conditions (including facultative anaerobes)no96.218yes
69480aerobicaerobicAbility to grow under oxygenic conditions (including facultative aerobes)yes71.729no
69480spore-formingspore-formingAbility to form endo- or exosporesno91.311yes
69480thermophilicthermophileAbility to grow at temperatures above or equal to 45°Cno97.5yes
69480flagellatedmotile2+Ability to perform flagellated movementyes86.491no

External links

@ref: 5732

culture collection no.: DSM 15157, CCM 7048, CIP 107627, NCIMB 13907

straininfo link

  • @ref: 70157
  • straininfo: 127997

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny13130022Marinobacter lipolyticus sp. nov., a novel moderate halophile with lipolytic activity.Martin S, Marquez MC, Sanchez-Porro C, Mellado E, Arahal DR, Ventosa AInt J Syst Evol Microbiol10.1099/ijs.0.02528-02003Alteromonadaceae/classification/genetics/*isolation & purification/metabolism, Base Composition, DNA, Bacterial/chemistry/genetics, DNA, Ribosomal/genetics, Lipolysis, Molecular Sequence Data, Phenotype, Phylogeny, RNA, Bacterial/genetics, RNA, Ribosomal, 16S/genetics, Sodium Chloride, Soil Microbiology, SpainGenetics
Phylogeny26518711Marinobacter aromaticivorans sp. nov., a polycyclic aromatic hydrocarbon-degrading bacterium isolated from sea sediment.Cui Z, Gao W, Xu G, Luan X, Li Q, Yin X, Huang D, Zheng LInt J Syst Evol Microbiol10.1099/ijsem.0.0007222015Base Composition, China, DNA, Bacterial/genetics, Fatty Acids/chemistry, Geologic Sediments/microbiology, Marinobacter/*classification/genetics/isolation & purification, Molecular Sequence Data, Nucleic Acid Hybridization, Phospholipids/chemistry, *Phylogeny, Polycyclic Aromatic Hydrocarbons/*metabolism, RNA, Ribosomal, 16S/genetics, Seawater/*microbiology, Sequence Analysis, DNA, Vitamin K 2/analogs & derivatives/chemistryGenetics

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
5732Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 15157)https://www.dsmz.de/collection/catalogue/details/culture/DSM-15157
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
23296S. Martín, M. C. Márquez, C. Sánchez-Porro, E. Mellado, D. R. Arahal, A. Ventosa10.1099/ijs.0.02528-0Marinobacter lipolyticus sp. nov., a novel moderate halophile with lipolytic activityIJSEM 53: 1383-1387 200313130022
36164Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/5084
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
70157Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID127997.1StrainInfo: A central database for resolving microbial strain identifiers
120621Curators of the CIPCollection of Institut Pasteur (CIP 107627)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20107627