Strain identifier
BacDive ID: 427
Type strain: 
Species: Alicyclobacillus sendaiensis
Strain Designation: NTAP-1
Strain history: T. Nakayama NTAP-1 <-- N. Tsuruoka.
NCBI tax ID(s): 1220572 (strain), 192387 (species)
version 8.1 (current version)
General
@ref: 7087
BacDive-ID: 427
DSM-Number: 17614
keywords: genome sequence, 16S sequence, Bacteria, spore-forming, thermophilic, Gram-positive
description: Alicyclobacillus sendaiensis NTAP-1 is a spore-forming, thermophilic, Gram-positive bacterium that was isolated from soil.
NCBI tax id
| NCBI tax id | Matching level |
|---|---|
| 1220572 | strain |
| 192387 | species |
strain history
| @ref | history |
|---|---|
| 7087 | <- JCM |
| 67770 | T. Nakayama NTAP-1 <-- N. Tsuruoka. |
doi: 10.13145/bacdive427.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/bacillota
- domain: Bacteria
- phylum: Bacillota
- class: Bacilli
- order: Caryophanales
- family: Alicyclobacillaceae
- genus: Alicyclobacillus
- species: Alicyclobacillus sendaiensis
- full scientific name: Alicyclobacillus sendaiensis Tsuruoka et al. 2003
@ref: 7087
domain: Bacteria
phylum: Firmicutes
class: Bacilli
order: Bacillales
family: Alicyclobacillaceae
genus: Alicyclobacillus
species: Alicyclobacillus sendaiensis
full scientific name: Alicyclobacillus sendaiensis Tsuruoka et al. 2003
strain designation: NTAP-1
type strain: yes
Morphology
cell morphology
- @ref: 69480
- gram stain: positive
- confidence: 100
Culture and growth conditions
culture medium
- @ref: 7087
- name: ALICYCLOBACILLUS MEDIUM (DSMZ Medium 402)
- growth: yes
- link: https://mediadive.dsmz.de/medium/402
- composition: Name: ALICYCLOBACILLUS MEDIUM (DSMZ Medium 402) Composition: Agar 15.0 g/l Glucose 5.0 g/l KH2PO4 3.0 g/l Yeast extract 2.0 g/l MgSO4 x 7 H2O 0.5 g/l CaCl2 x 2 H2O 0.25 g/l (NH4)2SO4 0.2 g/l H3BO3 0.0003 g/l CoCl2 x 6 H2O 0.0002 g/l ZnSO4 x 7 H2O 0.0001 g/l MnCl2 x 4 H2O 3e-05 g/l Na2MoO4 x 2 H2O 3e-05 g/l NiCl2 x 6 H2O 2e-05 g/l CuCl2 x 2 H2O 1e-05 g/l Distilled water
culture temp
| @ref | growth | type | temperature | range | confidence |
|---|---|---|---|---|---|
| 7087 | positive | growth | 50 | thermophilic | |
| 67770 | positive | growth | 55 | thermophilic | |
| 69480 | thermophilic | 99.998 |
Physiology and metabolism
spore formation
- @ref: 69480
- spore formation: yes
- confidence: 99.447
observation
- @ref: 67770
- observation: quinones: MK-7
Isolation, sampling and environmental information
isolation
| @ref | sample type | geographic location | country | origin.country | continent |
|---|---|---|---|---|---|
| 7087 | soil | Aoba-yama Park, Sendai | Japan | JPN | Asia |
| 67770 | Soil of Aoba-yama Park | Sendai | Japan | JPN | Asia |
isolation source categories
- Cat1: #Environmental
- Cat2: #Terrestrial
- Cat3: #Soil
taxonmaps
- @ref: 69479
- File name: preview.99_5561.png
- url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_313;96_1262;97_1497;98_4149;99_5561&stattab=map
- Last taxonomy: Alicyclobacillus sendaiensis
- 16S sequence: AB084128
- Sequence Identity:
- Total samples: 105
- soil counts: 30
- aquatic counts: 23
- animal counts: 37
- plant counts: 15
Safety information
risk assessment
- @ref: 7087
- biosafety level: 1
- biosafety level comment: Risk group (German classification)
Sequence information
16S sequences
| @ref | description | accession | length | database | NCBI tax ID |
|---|---|---|---|---|---|
| 20218 | Alicyclobacillus sendaiensis gene for 16S rRNA, partial sequence | AB222247 | 1523 | ena | 192387 |
| 7087 | Alicyclobacillus sendaiensis gene for 16S rRNA | AB084128 | 1438 | ena | 192387 |
Genome sequences
| @ref | description | accession | assembly level | database | NCBI tax ID |
|---|---|---|---|---|---|
| 66792 | Alicyclobacillus sendaiensis NBRC 100866 | 1220572.3 | wgs | patric | 1220572 |
| 66792 | Alicyclobacillus sendaiensis NBRC 100866 | 2731957596 | draft | img | 1220572 |
| 67770 | Alicyclobacillus sendaiensis NBRC 100866 | GCA_001552675 | contig | ncbi | 1220572 |
GC content
| @ref | GC-content | method |
|---|---|---|
| 7087 | 62.3 | |
| 67770 | 62.3 | high performance liquid chromatography (HPLC) |
Genome-based predictions
predictions
| trait | prediction | confidence | training_data |
|---|---|---|---|
| spore-forming | yes | 76 | no |
| motile | yes | 82.511 | no |
| gram-positive | yes | 85.233 | no |
| anaerobic | no | 97.384 | no |
| aerobic | yes | 90.908 | no |
| halophile | no | 91.281 | no |
| spore-forming | yes | 95.944 | no |
| glucose-util | yes | 90.463 | no |
| flagellated | no | 70.294 | no |
| thermophile | yes | 100 | no |
| glucose-ferment | no | 89.685 | no |
External links
@ref: 7087
culture collection no.: DSM 17614, ATCC BAA 609, JCM 11817, KCTC 3843, NBRC 100866
straininfo link
- @ref: 70106
- straininfo: 111788
literature
| topic | Pubmed-ID | title | authors | journal | DOI | year | mesh | topic2 |
|---|---|---|---|---|---|---|---|---|
| Enzymology | 12513991 | Collagenolytic serine-carboxyl proteinase from Alicyclobacillus sendaiensis strain NTAP-1: purification, characterization, gene cloning, and heterologous expression. | Tsuruoka N, Nakayama T, Ashida M, Hemmi H, Nakao M, Minakata H, Oyama H, Oda K, Nishino T | Appl Environ Microbiol | 10.1128/AEM.69.1.162-169.2003 | 2003 | Amino Acid Sequence, Animals, *Aspartic Acid Endopeptidases/chemistry/genetics/isolation & purification/metabolism, Bacteria, Aerobic/*enzymology/genetics, Cloning, Molecular, Collagen/*metabolism, Enzyme Stability, Molecular Sequence Data, Peptides/metabolism, Protease Inhibitors/pharmacology, Recombinant Proteins/genetics/metabolism, Sequence Analysis, DNA, Serine/metabolism, Substrate Specificity | Metabolism |
| Phylogeny | 12892130 | Alicyclobacillus sendaiensis sp. nov., a novel acidophilic, slightly thermophilic species isolated from soil in Sendai, Japan. | Tsuruoka N, Isono Y, Shida O, Hemmi H, Nakayama T, Nishino T | Int J Syst Evol Microbiol | 10.1099/ijs.0.02409-0 | 2003 | Bacillus/*classification/genetics/isolation & purification/metabolism, Collagenases/metabolism, DNA, Bacterial/genetics, DNA, Ribosomal/genetics, Fatty Acids/metabolism, Genotype, Gram-Positive Endospore-Forming Rods/*classification/genetics/isolation & purification/metabolism, Hydrogen-Ion Concentration, Japan, Molecular Sequence Data, Phenotype, Phylogeny, RNA, Bacterial/genetics, RNA, Ribosomal, 16S/genetics, *Soil Microbiology, Temperature | Metabolism |
| Metabolism | 31307580 | Efficient production of gluten hydrolase Kuma030 in E. coli by hot acid treatment without chromatography. | Liu H, Fan X, Song H, Hu X, Zhang G, Yu C, Yi L | Enzyme Microb Technol | 10.1016/j.enzmictec.2019.05.013 | 2019 | Alicyclobacillus/*enzymology/genetics, Bacterial Proteins/chemistry/genetics/*metabolism, Enzyme Stability, Escherichia coli/genetics/*metabolism, Glutens/*metabolism, Hot Temperature, Hydrolases/chemistry/genetics/*metabolism | Enzymology |
Reference
| @id | authors | catalogue | doi/url | title | journal | pubmed |
|---|---|---|---|---|---|---|
| 7087 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 17614) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-17614 | |||
| 20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | |||
| 20218 | Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P. | 10.1016/j.syapm.2013.11.002 | StrainInfo introduces electronic passports for microorganisms. | Syst Appl Microbiol. 37: 42-50 2014 | 24321274 | |
| 66792 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | |||
| 67770 | Curators of the JCM | https://jcm.brc.riken.jp/en/ | ||||
| 69479 | João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich. | https://microbeatlas.org/ | MicrobeAtlas 1.0 beta | |||
| 69480 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | |||
| 70106 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID111788.1 | StrainInfo: A central database for resolving microbial strain identifiers |