Strain identifier
BacDive ID: 4267
Type strain: 
Species: Alkalilimnicola ehrlichii
Strain Designation: MLHE-1
Strain history: <- S. E. Hoeft; MLHE-1
NCBI tax ID(s): 351052 (species)
version 8.1 (current version)
General
@ref: 7123
BacDive-ID: 4267
DSM-Number: 17681
keywords: genome sequence, 16S sequence, Bacteria, facultative anaerobe, mesophilic, Gram-negative, motile, rod-shaped
description: Alkalilimnicola ehrlichii MLHE-1 is a facultative anaerobe, mesophilic, Gram-negative bacterium that was isolated from anoxic bottom water.
NCBI tax id
- NCBI tax id: 351052
- Matching level: species
strain history
- @ref: 7123
- history: <- S. E. Hoeft; MLHE-1
doi: 10.13145/bacdive4267.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/pseudomonadota
- domain: Bacteria
- phylum: Pseudomonadota
- class: Gammaproteobacteria
- order: Chromatiales
- family: Ectothiorhodospiraceae
- genus: Alkalilimnicola
- species: Alkalilimnicola ehrlichii
- full scientific name: Alkalilimnicola ehrlichii Hoeft et al. 2007
@ref: 7123
domain: Bacteria
phylum: Proteobacteria
class: Gammaproteobacteria
order: Chromatiales
family: Ectothiorhodospiraceae
genus: Alkalilimnicola
species: Alkalilimnicola ehrlichii
full scientific name: Alkalilimnicola ehrlichii Hoeft et al. 2007
strain designation: MLHE-1
type strain: yes
Morphology
cell morphology
- @ref: 31874
- gram stain: negative
- cell length: 2 µm
- cell width: 0.4 µm
- cell shape: rod-shaped
- motility: yes
pigmentation
- @ref: 31874
- production: no
Culture and growth conditions
culture medium
- @ref: 7123
- name: ALCALILIMNICOLA EHRLICHII MLHE-1 (DSMZ Medium 1457)
- growth: yes
- link: https://mediadive.dsmz.de/medium/1457
- composition: Name: ALCALILIMNICOLA EHRLICHII MHLE-1 MEDIUM (DSMZ Medium 1457) Composition: NaCl 59.6423 g/l Agar 19.8807 g/l Na2CO3 10.5368 g/l NaHCO3 4.17495 g/l K2HPO4 0.149105 g/l (NH4)2SO4 0.0994036 g/l KH2PO4 0.0795227 g/l MgSO4 0.0248509 g/l HCl 0.0124254 g/l Na-acetate 0.00815445 g/l FeCl2 x 4 H2O 0.00745527 g/l CoCl2 x 6 H2O 0.000944334 g/l MnCl2 x 4 H2O 0.000497018 g/l ZnCl2 0.000347913 g/l Na2MoO4 x 2 H2O 0.000178926 g/l NiCl2 x 6 H2O 0.000119284 g/l H3BO3 2.98211e-05 g/l Pyridoxine hydrochloride 9.94036e-06 g/l CuCl2 x 2 H2O 9.94036e-06 g/l D-Calcium pantothenate 4.97018e-06 g/l Nicotinic acid 4.97018e-06 g/l Riboflavin 4.97018e-06 g/l Thiamine-HCl x 2 H2O 4.97018e-06 g/l Lipoic acid 4.97018e-06 g/l p-Aminobenzoic acid 4.97018e-06 g/l Tungstate 3.66583e-06 g/l Folic acid 1.98807e-06 g/l Biotin 1.98807e-06 g/l Vitamin B12 9.94036e-08 g/l Distilled water
culture temp
| @ref | growth | type | temperature | range |
|---|---|---|---|---|
| 7123 | positive | growth | 30 | mesophilic |
| 31874 | positive | growth | 13-40 | |
| 31874 | positive | optimum | 30 | mesophilic |
culture pH
| @ref | ability | type | pH | PH range |
|---|---|---|---|---|
| 31874 | positive | growth | 7.3-10.0 | alkaliphile |
| 31874 | positive | optimum | 9.3 |
Physiology and metabolism
oxygen tolerance
- @ref: 31874
- oxygen tolerance: facultative anaerobe
metabolite utilization
- @ref: 31874
- Chebi-ID: 17632
- metabolite: nitrate
- utilization activity: +
- kind of utilization tested: reduction
Isolation, sampling and environmental information
isolation
- @ref: 7123
- sample type: anoxic bottom water
- geographic location: California, Mono Lake
- country: USA
- origin.country: USA
- continent: North America
isolation source categories
| Cat1 | Cat2 |
|---|---|
| #Environmental | #Aquatic |
| #Condition | #Anoxic (anaerobic) |
taxonmaps
- @ref: 69479
- File name: preview.99_5634.png
- url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_15363;96_2712;97_3332;98_4200;99_5634&stattab=map
- Last taxonomy: Ectothiorhodospiraceae
- 16S sequence: AF406554
- Sequence Identity:
- Total samples: 574
- soil counts: 248
- aquatic counts: 232
- animal counts: 65
- plant counts: 29
Safety information
risk assessment
- @ref: 7123
- biosafety level: 1
- biosafety level comment: Risk group (German classification)
Sequence information
16S sequences
- @ref: 7123
- description: Alkalilimnicola ehrlichii MLHE-1 16S ribosomal RNA gene, partial sequence
- accession: AF406554
- length: 1461
- database: ena
- NCBI tax ID: 187272
Genome sequences
| @ref | description | accession | assembly level | database | NCBI tax ID |
|---|---|---|---|---|---|
| 66792 | Alkalilimnicola ehrlichii MLHE-1 | GCA_000014785 | complete | ncbi | 187272 |
| 66792 | Alkalilimnicola ehrlichii MLHE-1 | 187272.10 | complete | patric | 187272 |
| 66792 | Alkalilimnicola ehrlichii MLHE-1 | 637000005 | complete | img | 187272 |
GC content
- @ref: 7123
- GC-content: 67.5
Genome-based predictions
predictions
| trait | prediction | confidence | training_data |
|---|---|---|---|
| motile | yes | 87.348 | no |
| flagellated | yes | 80.793 | no |
| gram-positive | no | 99.246 | yes |
| anaerobic | no | 86.692 | yes |
| halophile | yes | 64.469 | no |
| spore-forming | no | 94.837 | no |
| thermophile | no | 90.363 | yes |
| glucose-util | yes | 58.301 | no |
| aerobic | no | 78.3 | no |
| glucose-ferment | no | 87.025 | no |
External links
@ref: 7123
culture collection no.: DSM 17681, ATCC BAA 1101
straininfo link
- @ref: 73762
- straininfo: 297118
literature
| topic | Pubmed-ID | title | authors | journal | DOI | year | mesh | topic2 |
|---|---|---|---|---|---|---|---|---|
| Metabolism | 17329775 | Alkalilimnicola ehrlichii sp. nov., a novel, arsenite-oxidizing haloalkaliphilic gammaproteobacterium capable of chemoautotrophic or heterotrophic growth with nitrate or oxygen as the electron acceptor. | Hoeft SE, Blum JS, Stolz JF, Tabita FR, Witte B, King GM, Santini JM, Oremland RS | Int J Syst Evol Microbiol | 10.1099/ijs.0.64576-0 | 2007 | Arsenites/metabolism, Carbon Monoxide/metabolism, Chemoautotrophic Growth, Electrons, Gammaproteobacteria/*classification/growth & development/*metabolism/ultrastructure, Genes, Bacterial, Heterotrophic Processes, Methane/metabolism, Nitrates/*metabolism, Oxygen/*metabolism, Phylogeny, Ribulose-Bisphosphate Carboxylase/metabolism | Phylogeny |
| Metabolism | 20453090 | Identification of a novel arsenite oxidase gene, arxA, in the haloalkaliphilic, arsenite-oxidizing bacterium Alkalilimnicola ehrlichii strain MLHE-1. | Zargar K, Hoeft S, Oremland R, Saltikov CW | J Bacteriol | 10.1128/JB.00244-10 | 2010 | Arsenites/*metabolism, Ectothiorhodospiraceae/*genetics/*metabolism, Gene Expression Profiling, Gene Expression Regulation, Bacterial/physiology, Gene Expression Regulation, Enzymologic/physiology, Oxidation-Reduction, Oxidoreductases/*genetics/*metabolism, Transcription, Genetic | Enzymology |
| Enzymology | 22404962 | ArxA, a new clade of arsenite oxidase within the DMSO reductase family of molybdenum oxidoreductases. | Zargar K, Conrad A, Bernick DL, Lowe TM, Stolc V, Hoeft S, Oremland RS, Stolz J, Saltikov CW | Environ Microbiol | 10.1111/j.1462-2920.2012.02722.x | 2012 | Arsenate Reductases/genetics, Arsenic/*metabolism, Autotrophic Processes, California, Ectothiorhodospira/*enzymology/genetics, Genes, Bacterial, Hot Springs/microbiology, Iron-Sulfur Proteins, Metagenome, Operon, Oxidation-Reduction, Oxidoreductases/*genetics, Phylogeny, Sequence Analysis, DNA | Phylogeny |
| Phylogeny | 25646538 | Identification of anaerobic arsenite-oxidizing and arsenate-reducing bacteria associated with an alkaline saline lake in Khovsgol, Mongolia. | Hamamura N, Itai T, Liu Y, Reysenbach AL, Damdinsuren N, Inskeep WP | Environ Microbiol Rep | 10.1111/1758-2229.12144 | 2014 | Arsenates/*metabolism, Arsenites/*metabolism, Bacteria/classification/genetics/*isolation & purification/*metabolism, Biodiversity, Hydrogen-Ion Concentration, Lakes/chemistry/*microbiology, Molecular Sequence Data, Mongolia, Oxidation-Reduction, Phylogeny | Metabolism |
| Metabolism | 25831529 | Carbon monoxide as a metabolic energy source for extremely halophilic microbes: implications for microbial activity in Mars regolith. | King GM | Proc Natl Acad Sci U S A | 10.1073/pnas.1424989112 | 2015 | Atmosphere, Bacteria/*metabolism, Carbon Dioxide/chemistry, Carbon Monoxide/*chemistry, Energy-Generating Resources, Extraterrestrial Environment, Halobacteriales/*metabolism, Mars, Molecular Sequence Data, *Soil Microbiology, Temperature, Water/chemistry | Genetics |
| Phylogeny | 26394793 | Spiribacter curvatus sp. nov., a moderately halophilic bacterium isolated from a saltern. | Leon MJ, Rodriguez-Olmos A, Sanchez-Porro C, Lopez-Perez M, Rodriguez-Valera F, Soliveri J, Ventosa A, Copa-Patino JL | Int J Syst Evol Microbiol | 10.1099/ijsem.0.000621 | 2015 | Base Composition, DNA, Bacterial/genetics, Ectothiorhodospiraceae/*classification/genetics/isolation & purification, Fatty Acids/chemistry, Molecular Sequence Data, Nucleic Acid Hybridization, *Phylogeny, Pigmentation, RNA, Ribosomal, 16S/genetics, *Salinity, Sequence Analysis, DNA, Spain, *Water Microbiology | Genetics |
| Phylogeny | 27470064 | Spiribacter roseus sp. nov., a moderately halophilic species of the genus Spiribacter from salterns. | Leon MJ, Vera-Gargallo B, Sanchez-Porro C, Ventosa A | Int J Syst Evol Microbiol | 10.1099/ijsem.0.001338 | 2016 | Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Ectothiorhodospiraceae/*classification/genetics/isolation & purification, Fatty Acids/chemistry, Nucleic Acid Hybridization, *Phylogeny, Pigmentation, RNA, Ribosomal, 16S/genetics, Salinity, Sequence Analysis, DNA, Spain, *Water Microbiology | Transcriptome |
Reference
| @id | authors | catalogue | doi/url | title | ID_cross_reference | pubmed | journal |
|---|---|---|---|---|---|---|---|
| 7123 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 17681) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-17681 | ||||
| 20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | ||||
| 31874 | Barberan A, Caceres Velazquez H, Jones S, Fierer N. | 10.1128/mSphere.00237-17 | Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information | 28134 | 28776041 | ||
| 66792 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | ||||
| 66794 | Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg | 10.1093/nar/gkaa1025 | BRENDA, the ELIXIR core data resource in 2021: new developments and updates | 33211880 | Nucleic Acids Res. 49: D498-D508 2020 | ||
| 69479 | João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich. | https://microbeatlas.org/ | MicrobeAtlas 1.0 beta | ||||
| 73762 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID297118.1 | StrainInfo: A central database for resolving microbial strain identifiers |