Alkalilimnicola ehrlichii (DSM 17681, ATCC BAA-1101, MLHE-1)

Name: Alkalilimnicola ehrlichii (DSM 17681, ATCC BAA-1101, MLHE-1)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 4267

Type strain

Strain Designation MLHE-1

Culture col. no. DSM 17681 ATCC BAA-1101 MLHE-1

NCBI tax ID(s) 187272 351052

Special Collections DSMZ Literature strains

History <- S. E. Hoeft; MLHE-1

Links

Alkalilimnicola ehrlichii MLHE-1 is a facultative anaerobe, Gram-negative, motile bacterium that was isolated from anoxic bottom water.

Gram-negative motile rod-shaped facultative anaerobe genome sequence 16S sequence Bacteria

Name and taxonomic classification

SI-ID 297118

ATCC BAA-1101,DSM 17681

@ref 20215

Last LPSN update 2026-02-09 11:18:41

Domain Bacteria

Phylum Pseudomonadota

Class Gammaproteobacteria

Order Chromatiales

Family Ectothiorhodospiraceae

Genus Alkalilimnicola

Species Alkalilimnicola ehrlichii

Full scientific name Alkalilimnicola ehrlichii Hoeft et al. 2007

Morphology

Cell morphology

@ref	Gram stain	Cell length	Cell width	Cell shape	Confidence
31874	negative	2 µm	0.4 µm	rod-shaped
125438	negative				98.75
125439	negative				96

Pigmentation

31874

Productionno

Culture and growth conditions

Culture medium

@ref	Name	Growth	Medium link	Composition
7123	ALCALILIMNICOLA EHRLICHII MLHE-1 (DSMZ Medium 1457)		Medium recipe at MediaDive	Name: ALCALILIMNICOLA EHRLICHII MHLE-1 MEDIUM (DSMZ Medium 1457) Composition: NaCl 59.6423 g/l Agar 19.8807 g/l Na2CO3 10.5368 g/l NaHCO3 4.17495 g/l K2HPO4 0.149105 g/l (NH4)2SO4 0.0994036 g/l KH2PO4 0.0795227 g/l MgSO4 0.0248509 g/l HCl 0.0124254 g/l Na-acetate 0.00815445 g/l FeCl2 x 4 H2O 0.00745527 g/l CoCl2 x 6 H2O 0.000944334 g/l MnCl2 x 4 H2O 0.000497018 g/l ZnCl2 0.000347913 g/l Na2MoO4 x 2 H2O 0.000178926 g/l NiCl2 x 6 H2O 0.000119284 g/l H3BO3 2.98211e-05 g/l Pyridoxine hydrochloride 9.94036e-06 g/l CuCl2 x 2 H2O 9.94036e-06 g/l D-Calcium pantothenate 4.97018e-06 g/l Nicotinic acid 4.97018e-06 g/l Riboflavin 4.97018e-06 g/l Thiamine-HCl x 2 H2O 4.97018e-06 g/l Lipoic acid 4.97018e-06 g/l p-Aminobenzoic acid 4.97018e-06 g/l Tungstate 3.66583e-06 g/l Folic acid 1.98807e-06 g/l Biotin 1.98807e-06 g/l Vitamin B12 9.94036e-08 g/l Distilled water

Culture temp

@ref	Growth	Type	Temperature (°C)
7123	positive	growth	30
31874	positive	growth	13-40
31874	positive	optimum	30

Culture pH

@ref	Ability	Type	PH	PH range
31874	positive	optimum	9.3
31874	positive	growth	7.3-10.0	alkaliphile

Physiology and metabolism

Oxygen tolerance

31874

Oxygen tolerancefacultative anaerobe

Spore formation

@ref	Spore formation	Confidence
125438		92.081
125439		94.9

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
31874	17632 ChEBI	nitrate	+	reduction

Pathway annotation

Computationally determined by

@ref	pathway	enzyme coverage	annotated reactions
66794	cellulose degradation	100	5 of 5
66794	cis-vaccenate biosynthesis	100	2 of 2
66794	formaldehyde oxidation	100	3 of 3
66794	cardiolipin biosynthesis	100	7 of 7
66794	L-lactaldehyde degradation	100	3 of 3
66794	enterobactin biosynthesis	100	3 of 3
66794	palmitate biosynthesis	100	22 of 22
66794	methylglyoxal degradation	100	5 of 5
66794	adipate degradation	100	2 of 2
66794	acetate fermentation	100	4 of 4
66794	biotin biosynthesis	100	4 of 4
66794	coenzyme A metabolism	100	4 of 4
66794	suberin monomers biosynthesis	100	2 of 2
66794	folate polyglutamylation	100	1 of 1
66794	ceramide biosynthesis	100	1 of 1
66794	denitrification	100	2 of 2
66794	anapleurotic synthesis of oxalacetate	100	1 of 1
66794	CDP-diacylglycerol biosynthesis	100	2 of 2
66794	UDP-GlcNAc biosynthesis	100	3 of 3
66794	NAD metabolism	94.44	17 of 18
66794	phenylalanine metabolism	92.31	12 of 13
66794	aspartate and asparagine metabolism	88.89	8 of 9
66794	valine metabolism	88.89	8 of 9
66794	ubiquinone biosynthesis	85.71	6 of 7
66794	tetrahydrofolate metabolism	85.71	12 of 14
66794	Entner Doudoroff pathway	80	8 of 10
66794	phenylacetate degradation (aerobic)	80	4 of 5
66794	heme metabolism	78.57	11 of 14
66794	glutamate and glutamine metabolism	78.57	22 of 28
66794	molybdenum cofactor biosynthesis	77.78	7 of 9
66794	serine metabolism	77.78	7 of 9
66794	vitamin B1 metabolism	76.92	10 of 13
66794	butanoate fermentation	75	3 of 4
66794	C4 and CAM-carbon fixation	75	6 of 8
66794	ppGpp biosynthesis	75	3 of 4
66794	glycogen biosynthesis	75	3 of 4
66794	sulfopterin metabolism	75	3 of 4
66794	CMP-KDO biosynthesis	75	3 of 4
66794	gluconeogenesis	75	6 of 8
66794	peptidoglycan biosynthesis	73.33	11 of 15
66794	proline metabolism	72.73	8 of 11
66794	glutathione metabolism	71.43	10 of 14
66794	photosynthesis	71.43	10 of 14
66794	lipid metabolism	70.97	22 of 31
66794	glycolysis	70.59	12 of 17
66794	purine metabolism	70.21	66 of 94
66794	starch degradation	70	7 of 10
66794	threonine metabolism	70	7 of 10
66794	alanine metabolism	68.97	20 of 29
66794	acetyl CoA biosynthesis	66.67	2 of 3
66794	chorismate metabolism	66.67	6 of 9
66794	octane oxidation	66.67	2 of 3
66794	acetoin degradation	66.67	2 of 3
66794	degradation of aromatic, nitrogen containing compounds	66.67	8 of 12
66794	d-mannose degradation	66.67	6 of 9
66794	cyanate degradation	66.67	2 of 3
66794	CO2 fixation in Crenarchaeota	66.67	6 of 9
66794	6-hydroxymethyl-dihydropterin diphosphate biosynthesis	62.5	5 of 8
66794	arginine metabolism	62.5	15 of 24
66794	pyrimidine metabolism	62.22	28 of 45
66794	reductive acetyl coenzyme A pathway	57.14	4 of 7
66794	degradation of sugar alcohols	56.25	9 of 16
66794	lipid A biosynthesis	55.56	5 of 9
66794	non-pathway related	55.26	21 of 38
66794	d-xylose degradation	54.55	6 of 11
66794	vitamin B6 metabolism	54.55	6 of 11
66794	leucine metabolism	53.85	7 of 13
66794	flavin biosynthesis	53.33	8 of 15
66794	lysine metabolism	52.38	22 of 42
66794	cysteine metabolism	50	9 of 18
66794	kanosamine biosynthesis II	50	1 of 2
66794	phenylmercury acetate degradation	50	1 of 2
66794	toluene degradation	50	2 of 4
66794	tyrosine metabolism	50	7 of 14
66794	citric acid cycle	50	7 of 14
66794	glycine metabolism	50	5 of 10
66794	dTDPLrhamnose biosynthesis	50	4 of 8
66794	arachidonic acid metabolism	50	9 of 18
66794	pantothenate biosynthesis	50	3 of 6
66794	cyclohexanol degradation	50	2 of 4
66794	isoprenoid biosynthesis	50	13 of 26
66794	methionine metabolism	50	13 of 26
66794	dolichol and dolichyl phosphate biosynthesis	50	1 of 2
66794	glycolate and glyoxylate degradation	50	3 of 6
66794	isoleucine metabolism	50	4 of 8
66794	ketogluconate metabolism	50	4 of 8
66794	aminopropanol phosphate biosynthesis	50	1 of 2
66794	ethanol fermentation	50	1 of 2
66794	histidine metabolism	48.28	14 of 29
66794	polyamine pathway	47.83	11 of 23
66794	tryptophan metabolism	47.37	18 of 38
66794	urea cycle	46.15	6 of 13
66794	sulfate reduction	46.15	6 of 13
66794	pentose phosphate pathway	45.45	5 of 11
66794	allantoin degradation	44.44	4 of 9
66794	propanol degradation	42.86	3 of 7
66794	oxidative phosphorylation	42.86	39 of 91
66794	vitamin B12 metabolism	41.18	14 of 34
66794	O-antigen biosynthesis	40	2 of 5
66794	vitamin K metabolism	40	2 of 5
66794	lipoate biosynthesis	40	2 of 5
66794	factor 420 biosynthesis	40	2 of 5
66794	propionate fermentation	40	4 of 10
66794	glycogen metabolism	40	2 of 5
66794	coenzyme M biosynthesis	40	4 of 10
66794	arachidonate biosynthesis	40	2 of 5
66794	glycine betaine biosynthesis	40	2 of 5
66794	degradation of hexoses	38.89	7 of 18
66794	phosphatidylethanolamine bioynthesis	38.46	5 of 13
66794	androgen and estrogen metabolism	37.5	6 of 16
66794	carnitine metabolism	37.5	3 of 8
66794	cholesterol biosynthesis	36.36	4 of 11
66794	degradation of pentoses	35.71	10 of 28
66794	IAA biosynthesis	33.33	1 of 3
66794	nitrate assimilation	33.33	3 of 9
66794	methane metabolism	33.33	1 of 3
66794	myo-inositol biosynthesis	30	3 of 10
66794	benzoyl-CoA degradation	28.57	2 of 7
66794	metabolism of disaccharids	27.27	3 of 11
66794	dolichyl-diphosphooligosaccharide biosynthesis	27.27	3 of 11
66794	ascorbate metabolism	27.27	6 of 22
66794	3-phenylpropionate degradation	26.67	4 of 15
66794	lactate fermentation	25	1 of 4
66794	degradation of sugar acids	24	6 of 25
66794	bile acid biosynthesis, neutral pathway	23.53	4 of 17
66794	phenylpropanoid biosynthesis	23.08	3 of 13
66794	4-hydroxymandelate degradation	22.22	2 of 9

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2
#Environmental	#Aquatic
#Condition	#Anoxic (anaerobic)

Isolation

@ref	Sample type	Geographic location	Country	Country ISO 3 Code	Continent
7123	anoxic bottom water	California, Mono Lake	USA	USA	North America

Taxonmaps

69479

Global distribution of 16S sequence AF406554 (>99% sequence identity) for Ectothiorhodospiraceae from Microbeatlas

Aquatic Samples	232
Soil Samples	248
Animal Samples	65
Plant Samples	29
Total Samples	574

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
7123	1	Risk group (German classification) According to TRBA 466

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	IMG accession	NCBI tax ID	Score
66792	ASM1478v1 assembly for Alkalilimnicola ehrlichii MLHE-1	complete	GCA_000014785	187272.10	637000005	187272	97.61

16S sequences

@ref	Description	Accession	Length	Database	NCBI tax ID
7123	Alkalilimnicola ehrlichii MLHE-1 16S ribosomal RNA gene, partial sequence	AF406554	1461		187272

GC content

7123

GC-content (mol%)67.5

Genome-based predictions

Predictions

Predictions from Deep Learning for Genome Sequence Data. R package version 0.3.1 based on: Alkalilimnicola ehrlichii MLHE-1
PATRIC: 187272.10

@ref	Trait	Model	Prediction	Confidence in %	In training data
125439	spore_formation	BacteriaNetⓘ	no	94.90	no
125439	motility	BacteriaNetⓘ	yes	79.00	no
125439	gram_stain	BacteriaNetⓘ	negative	96.00	no
125439	oxygen_tolerance	BacteriaNetⓘ	obligate aerobe	69.80	no

Predictions from bacterial phenotypic traits through improved machine learning using high-quality, curated datasets based on: Alkalilimnicola ehrlichii MLHE-1 MLHE-1
NCBI: GCA_000014785.1

@ref	Trait	Model	Prediction	Confidence in %	In training data
125438	gram-positive	gram-positiveⓘ	no	98.75	yes
125438	anaerobic	anaerobicⓘ	no	86.09	yes
125438	aerobic	aerobicⓘ	no	58.03	yes
125438	spore-forming	spore-formingⓘ	no	92.08	no
125438	thermophilic	thermophileⓘ	no	90.66	yes
125438	flagellated	motile2+ⓘ	yes	83.33	no

Literature

Topic	Title	Authors	Journal	DOI	Year
	Complete genome sequences of Alkalilimnicola ehrlichii strain SGAR-12 isolated from a pilot-scale haloalkaline biodesulfurization installation and type strain A. ehrlichii MLHE^T.	Plugge CM, Gupta S, Garcia Villa S, Timmers PHA, Muyzer G.	Microbiol Resour Announc	10.1128/mra.00567-25	2025
	Development of quantitative PCR for the detection of Alkalilimnicola ehrlichii, Thioalkalivibrio sulfidiphilus and Thioalkalibacter halophilus in gas biodesulfurization processes.	Kiragosyan K, van Veelen P, Gupta S, Tomaszewska-Porada A, Roman P, Timmers PHA.	AMB Express	10.1186/s13568-019-0826-1	2019
Metabolism	Carbon monoxide as a metabolic energy source for extremely halophilic microbes: implications for microbial activity in Mars regolith.	King GM	Proc Natl Acad Sci U S A	10.1073/pnas.1424989112	2015
Phylogeny	Identification of anaerobic arsenite-oxidizing and arsenate-reducing bacteria associated with an alkaline saline lake in Khovsgol, Mongolia.	Hamamura N, Itai T, Liu Y, Reysenbach AL, Damdinsuren N, Inskeep WP	Environ Microbiol Rep	10.1111/1758-2229.12144	2014
Enzymology	ArxA, a new clade of arsenite oxidase within the DMSO reductase family of molybdenum oxidoreductases.	Zargar K, Conrad A, Bernick DL, Lowe TM, Stolc V, Hoeft S, Oremland RS, Stolz J, Saltikov CW	Environ Microbiol	10.1111/j.1462-2920.2012.02722.x	2012
Metabolism	Identification of a novel arsenite oxidase gene, arxA, in the haloalkaliphilic, arsenite-oxidizing bacterium Alkalilimnicola ehrlichii strain MLHE-1.	Zargar K, Hoeft S, Oremland R, Saltikov CW	J Bacteriol	10.1128/JB.00244-10	2010
Phylogeny	Spiribacter roseus sp. nov., a moderately halophilic species of the genus Spiribacter from salterns.	Leon MJ, Vera-Gargallo B, Sanchez-Porro C, Ventosa A	Int J Syst Evol Microbiol	10.1099/ijsem.0.001338	2016
Phylogeny	Spiribacter curvatus sp. nov., a moderately halophilic bacterium isolated from a saltern.	Leon MJ, Rodriguez-Olmos A, Sanchez-Porro C, Lopez-Perez M, Rodriguez-Valera F, Soliveri J, Ventosa A, Copa-Patino JL	Int J Syst Evol Microbiol	10.1099/ijsem.0.000621	2015
Metabolism	Alkalilimnicola ehrlichii sp. nov., a novel, arsenite-oxidizing haloalkaliphilic gammaproteobacterium capable of chemoautotrophic or heterotrophic growth with nitrate or oxygen as the electron acceptor.	Hoeft SE, Blum JS, Stolz JF, Tabita FR, Witte B, King GM, Santini JM, Oremland RS	Int J Syst Evol Microbiol	10.1099/ijs.0.64576-0	2007

References

#7123	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 17681
#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#28134	IJSEM 504 2007 ( DOI 10.1099/ijs.0.64576-0 , PubMed 17329775 )
#31874	Barberan A, Caceres Velazquez H, Jones S, Fierer N.: Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information. mSphere 2: 2017 ( DOI 10.1128/mSphere.00237-17 , PubMed 28776041 ) - originally annotated from #28134
#66792	Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .
#66794	Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg: BRENDA, the ELIXIR core data resource in 2021: new developments and updates. Nucleic Acids Res. 49: D498 - D508 2020 ( DOI 10.1093/nar/gkaa1025 , PubMed 33211880 )
#69479	João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .
#125438	Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann: Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets. 2024 ( DOI 10.1101/2024.08.12.607695 )
#125439	Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Alkalilimnicola ehrlichii (DSM 17681, ATCC BAA-1101, MLHE-1)

Name and taxonomic classification

Morphology

Cell morphology

Pigmentation

Culture and growth conditions

Culture medium

Culture temp

Culture pH

Physiology and metabolism

Oxygen tolerance

Spore formation

Metabolite utilization

Pathway annotation

Isolation, sampling and environmental information

Isolation source categories

Isolation

Taxonmaps

Interaction and safety

Risk assessment

Sequence information

Genome sequences

Genome browser: GCA_000014785

16S sequences

GC content

Genome-based predictions

Predictions

Literature

Literature

References

BacDive

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