Strain identifier

BacDive ID: 3187

Type strain: Yes

Species: Corynebacterium simulans

Strain Designation: Co 553

Strain history: CIP <- 2000, G. Wauters, UCL, Brussels, Belgium: strain Co 553 <- B. van Bosterhaut

NCBI tax ID(s): 146827 (species)

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General

@ref: 11538

BacDive-ID: 3187

DSM-Number: 44415

keywords: 16S sequence, Bacteria, facultative anaerobe, mesophilic, Gram-positive

description: Corynebacterium simulans Co 553 is a facultative anaerobe, mesophilic, Gram-positive bacterium that was isolated from human axillar lymh node.

NCBI tax id

  • NCBI tax id: 146827
  • Matching level: species

strain history

@refhistory
11538<- G. Wauters, UCL, Bruxelles; Co 553
67770CCUG 43305 <-- G. Wauters Co 553.
122623CIP <- 2000, G. Wauters, UCL, Brussels, Belgium: strain Co 553 <- B. van Bosterhaut

doi: 10.13145/bacdive3187.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Actinomycetes
  • order: Mycobacteriales
  • family: Corynebacteriaceae
  • genus: Corynebacterium
  • species: Corynebacterium simulans
  • full scientific name: Corynebacterium simulans Wattiau et al. 2000

@ref: 11538

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Actinomycetales

family: Corynebacteriaceae

genus: Corynebacterium

species: Corynebacterium simulans

full scientific name: Corynebacterium simulans Wattiau et al. 2000

strain designation: Co 553

type strain: yes

Morphology

cell morphology

@refgram stainmotilitycell shape
23183positiveno
122623positivenorod-shaped

colony morphology

@refcolony colorincubation periodmedium usedcolony size
20057Ivory (1014)10-14 daysISP 2
20057Ivory (1014)10-14 daysISP 3
20057Beige (1001)10-14 daysISP 4
20057Colorless10-14 daysISP 5
20057Ivory (1014)10-14 daysISP 6
20057Colorless10-14 daysISP 7
23183greyish-white2 daysblood agar1-2 mm
122623

multicellular morphology

@refforms multicellular complexmedium name
20057noISP 2
20057noISP 3
20057noISP 4
20057noISP 5
20057noISP 6
20057noISP 7

multimedia

  • @ref: 11538
  • multimedia content: https://www.dsmz.de/microorganisms/photos/DSM_44415.jpg
  • caption: Medium 693 37°C
  • intellectual property rights: © Leibniz-Institut DSMZ

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
11538TRYPTICASE SOY BROTH AGAR+BLOOD (DSMZ Medium 535a)yeshttps://mediadive.dsmz.de/medium/535aName: TRYPTICASE SOY BROTH AGAR+BLOOD (DSMZ Medium 535a) Composition: None 50.0 g/l Trypticase soy broth 30.0 g/l Agar 15.0 g/l Distilled water
11538COLUMBIA BLOOD MEDIUM (DSMZ Medium 693)yeshttps://mediadive.dsmz.de/medium/693Name: COLUMBIA BLOOD MEDIUM (DSMZ Medium 693) Composition: Defibrinated sheep blood 50.0 g/l Columbia agar base
20057ISP 2yesName: ISP 2 / Yeast Malt Agar (5265); 5265 Composition Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l Agar 15.0 g/l Preparation: Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.0 Usage: Maintenance and Taxonomy Organisms: All Actinomycetes
20057ISP 3yesName: ISP 3; 5315 Composition Dog oat flakes 20.0 g/l Trace element solution (5314) 2.5 ml/l Agar 18.0 g/l Preparation: Oat flakes are cooked for 20 minutes, trace element solution and agar are added (in the case of non rolled oat flakes the suspension has to bee filtrated). Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.8 Usage: Maintenance and taxonomy (e.g. SEM As liquid medium for metabolite production) Organisms: All Actinomycetes Trace element solution 5314 Name: Trace element solution 5314; 5314 Composition CaCl2 x H2O 3.0 g/l Fe-III-citrate 1.0 g/l MnSO4 0.2 g/l ZnCl2 0.1 g/l CuSO4 x 5 H2O 0.025 g/l Sodium tetra borate 0.2 g/l CoCl2 x 6 H2O 0.004 g/l Sodium molybdate 0.01 g/l Preparation: Use double destillated water. Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Trace element solution for different media Organisms:
20057ISP 4yesName: ISP 4; DSM 547 Solution I: Difco soluble starch, 10.0 g. Make a paste of the starch with a small amount of cold distilled water and bring to a volume of 500 ml. Solution II: CaCO3 2.0 g K2HPO4 (anhydrous) 1.0 g MgSO4 x 7 H2O 1.0 g NaCl 1.0 g (NH4)2SO4 2.0 g Distilled water 500.0 ml Trace salt solution (see below) 1.0 ml The pH should be between 7.0 and 7.4. Do not adjust if it is within this range. Mix solutions I and II together. Add 20.0 g agar. Liquify agar by steaming at 100°C for 10 to 20 min. Trace element solution: FeSO4 x 7 H2O 0.1 g MnCl2 x 4 H2O 0.1 g ZnSO4 x 7 H2O 0.1 g Distilled water 100.0 ml
20057ISP 5yesName: ISP 5 (5323) Composition L-Asparagine 1.0 g/l Glycerol 10.0 g/l K2HPO4 1.0 g/l Salt solution (see preparation) 1.0 ml/l Agar 20.0 g/l Preparation: Salt solution 1.0 g FeSO4 x 7 H2O 1.0 g MnCl2 x 4 H2O 1.0 g ZNSO4 x 7 H2O in 100 ml water Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.2 Usage: Maintenance and taxonomy Organisms: All Actinomycetes
20057ISP 6yesName: ISP 6 (5318) Composition Peptone 15.0 g/l Proteose peptose 5.0 g/l Ferric ammonium citrate 0.5 g/l Sodium glycerophosphate 1.0 g/l Sodium thiosulfate 0.08 g/l Yeast extract 1.0 g/l Agar 15.0 g/l Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Production of melanoid pigments Organisms: All Actinomycetes
20057ISP 7yesName: ISP 7 (5322) Composition Glycerol 15.0 g/l L-Tyrosine 0.5 g/l L-Asparagine 1.0 g/l K2HPO4 0.5 g/l NaCl 0.5 g/l FeSO4 x 7 H2O 0.01 g/l Trace element solution 5343 1.0 ml/l Agar 20.0 Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.3 Usage: Production of melanoid pigments Organisms: All Actinomycetes
23183blood agaryes
39539MEDIUM 6 - Columbia agar with 10 % horse bloodyesDistilled water make up to (1000.000 ml);Columbia agar (39.000 g);Horseblood (100.000 ml)
122623CIP Medium 6yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=6

culture temp

@refgrowthtypetemperaturerange
20057positiveoptimum37mesophilic
11538positivegrowth37mesophilic
23183positivegrowth37mesophilic
23183positiveminimum20psychrophilic
39539positivegrowth37mesophilic
67770positivegrowth37mesophilic
122623positivegrowth25-37mesophilic
122623nogrowth10psychrophilic
122623nogrowth15psychrophilic
122623nogrowth41thermophilic
122623nogrowth45thermophilic

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
23183facultative anaerobe
122623facultative anaerobe

spore formation

  • @ref: 23183
  • spore formation: no

halophily

  • @ref: 122623
  • salt: NaCl
  • growth: positive
  • tested relation: growth
  • concentration: 0-10 %

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
23183168082-dehydro-D-gluconate-builds acid from
23183174265-dehydro-D-gluconate-builds acid from
2318315963ribitol-builds acid from
2318327613amygdalin-builds acid from
2318318305arbutin-builds acid from
2318317057cellobiose-builds acid from
2318317108D-arabinose-builds acid from
2318318333D-arabitol-builds acid from
2318328847D-fucose-builds acid from
2318362318D-lyxose-builds acid from
2318316443D-tagatose-builds acid from
2318332528turanose-builds acid from
2318365327D-xylose-builds acid from
2318316813galactitol-builds acid from
2318317113erythritol-builds acid from
231834853esculin-builds acid from
2318330742ethylene glycol-builds acid from
2318328066gentiobiose-builds acid from
2318324265gluconate-builds acid from
2318317754glycerol-builds acid from
2318328087glycogen-builds acid from
2318317268myo-inositol-builds acid from
2318315443inulin-builds acid from
2318330849L-arabinose-builds acid from
2318318403L-arabitol-builds acid from
2318318287L-fucose-builds acid from
2318365328L-xylose-builds acid from
2318317716lactose-builds acid from
2318317306maltose-builds acid from
2318329864mannitol-builds acid from
231836731melezitose-builds acid from
2318328053melibiose-builds acid from
23183320061methyl alpha-D-glucopyranoside-builds acid from
2318343943methyl alpha-D-mannoside-builds acid from
2318374863methyl beta-D-xylopyranoside-builds acid from
2318316634raffinose-builds acid from
2318326546rhamnose-builds acid from
2318317814salicin-builds acid from
2318330911sorbitol-builds acid from
2318327922sorbose-builds acid from
2318328017starch-builds acid from
2318327082trehalose-builds acid from
2318317151xylitol-builds acid from
231834853esculin-hydrolysis
231835291gelatin-hydrolysis
2318316199urea-hydrolysis
2318328757fructose+builds acid from
2318317234glucose+builds acid from
2318337684mannose+builds acid from
2318317992sucrose+builds acid from
2318317632nitrate+reduction
2318316301nitrite+reduction
2318328260galactose+/-builds acid from
23183506227N-acetylglucosamine+/-builds acid from
2318333942ribose+/-builds acid from
68371Potassium 5-ketogluconate-builds acid from
68371Potassium 2-ketogluconate-builds acid from
6837124265gluconate-builds acid from
6837118403L-arabitol-builds acid from
6837118333D-arabitol-builds acid from
6837118287L-fucose-builds acid from
6837128847D-fucose-builds acid from
6837116443D-tagatose-builds acid from
6837162318D-lyxose-builds acid from
6837132528turanose-builds acid from
6837128066gentiobiose-builds acid from
6837117151xylitol-builds acid from
6837128087glycogen-builds acid from
6837128017starch-builds acid from
6837116634raffinose-builds acid from
683716731melezitose-builds acid from
6837115443inulin-builds acid from
6837127082trehalose-builds acid from
6837117992sucrose+builds acid from
6837128053melibiose-builds acid from
6837117716lactose-builds acid from
6837117306maltose-builds acid from
6837117057cellobiose-builds acid from
6837117814salicin-builds acid from
683714853esculin-builds acid from
6837118305arbutin-builds acid from
6837127613amygdalin-builds acid from
6837159640N-acetylglucosamine-builds acid from
68371320061methyl alpha-D-glucopyranoside-builds acid from
6837143943methyl alpha-D-mannoside-builds acid from
6837117924D-sorbitol-builds acid from
6837116899D-mannitol-builds acid from
6837117268myo-inositol-builds acid from
6837116813galactitol-builds acid from
6837162345L-rhamnose-builds acid from
6837117266L-sorbose-builds acid from
6837116024D-mannose+builds acid from
6837115824D-fructose+builds acid from
6837117634D-glucose+builds acid from
6837112936D-galactose+builds acid from
6837174863methyl beta-D-xylopyranoside-builds acid from
6837115963ribitol-builds acid from
6837165328L-xylose-builds acid from
6837165327D-xylose-builds acid from
6837116988D-ribose+builds acid from
6837130849L-arabinose-builds acid from
6837117108D-arabinose-builds acid from
6837117113erythritol-builds acid from
6837117754glycerol-builds acid from
1226234853esculin-hydrolysis
122623606565hippurate+hydrolysis
12262317632nitrate+reduction
12262316301nitrite+reduction
12262317632nitrate+respiration
683794853esculin-hydrolysis
6837916199urea-hydrolysis
683795291gelatin-hydrolysis
6837965327D-xylose-fermentation
6837916899D-mannitol-fermentation
6837917306maltose-fermentation
6837917716lactose-fermentation
6837928087glycogen-fermentation

metabolite production

  • @ref: 122623
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

metabolite tests

@refChebi-IDmetabolitevoges-proskauer-testmethylred-test
12262315688acetoin-
12262317234glucose-

enzymes

@refvalueactivityec
23183acid phosphatase-3.1.3.2
23183alkaline phosphatase+3.1.3.1
23183alpha-fucosidase-3.2.1.51
23183alpha-galactosidase-3.2.1.22
23183alpha-glucosidase-3.2.1.20
23183alpha-mannosidase-3.2.1.24
23183beta-galactosidase-3.2.1.23
23183beta-glucosidase-3.2.1.21
23183beta-glucuronidase-3.2.1.31
23183catalase+1.11.1.6
23183chymotrypsin-3.4.4.5
23183cystine arylamidase-3.4.11.3
23183esterase+
23183esterase lipase (C 8)+
23183leucine arylamidase+3.4.11.1
23183lipase-
23183N-acetyl-beta-glucosaminidase-3.2.1.52
23183naphthol-AS-BI-phosphohydrolase+
23183pyrazinamidase+/-3.5.1.B15
23183trypsin+3.4.21.4
23183valine arylamidase-
68382alpha-fucosidase-3.2.1.51
68382alpha-mannosidase-3.2.1.24
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382beta-glucosidase-3.2.1.21
68382beta-glucuronidase-3.2.1.31
68382beta-galactosidase-3.2.1.23
68382alpha-galactosidase-3.2.1.22
68382naphthol-AS-BI-phosphohydrolase+
68382acid phosphatase+3.1.3.2
68382alpha-chymotrypsin-3.4.21.1
68382cystine arylamidase-3.4.11.3
68382leucine arylamidase+3.4.11.1
68382lipase (C 14)-
68382esterase lipase (C 8)+
68382esterase (C 4)+
68382alkaline phosphatase+3.1.3.1
68379catalase+1.11.1.6
68379gelatinase-
68379urease-3.5.1.5
68379beta-glucosidase-3.2.1.21
68379N-acetyl-beta-glucosaminidase-3.2.1.52
68379alpha-glucosidase-3.2.1.20
68379beta-galactosidase-3.2.1.23
68379beta-glucuronidase-3.2.1.31
68379alkaline phosphatase+3.1.3.1
68379pyrrolidonyl arylamidase-3.4.19.3
68379pyrazinamidase-3.5.1.B15
122623oxidase+
122623beta-galactosidase-3.2.1.23
122623alcohol dehydrogenase-1.1.1.1
122623gelatinase-
122623amylase-
122623DNase-
122623caseinase-3.4.21.50
122623catalase+1.11.1.6
122623tween esterase+
122623gamma-glutamyltransferase-2.3.2.2
122623lecithinase-
122623lipase-
122623lysine decarboxylase-4.1.1.18
122623ornithine decarboxylase-4.1.1.17
122623phenylalanine ammonia-lyase-4.3.1.24
122623tryptophan deaminase-
122623urease-3.5.1.5

fatty acid profile

  • fatty acids

    @reffatty acidpercentageECL
    55780C16:021.616
    55780C18:0518
    55780C16:1 ω11c1.915.757
    55780C16:1 ω7c0.715.819
    55780C17:1 ω8c1216.792
    55780C18:1 ω9c57.417.769
    55780C18:2 ω6,9c/C18:0 ANTE0.917.724
    55780C19:1 ISO I0.518.473
  • type of FA analysis: whole cell analysis
  • method/protocol: CCUG

API coryne

@refNITPYZPYRAPALbeta GURbeta GALalpha GLUbeta NAGESCUREGELGLURIBXYLMANMALLACSACGLYGControlCAT
20057---+---------------
11538+/---+-------++----+--+

API zym

@refAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- FucosidaseControl
20057+++-+--+-++--------
122623+++-++---++---+-----

API 50CHac

@refGLYERYDARALARARIBDXYLLXYLADOMDXGALGLUFRUMNESBERHADULINOMANSORMDMMDGNAGAMYARBESCSALCELMALLACMELSACTREINUMLZRAFAMDGLYGXLTGENTURLYXTAGDFUCLFUCDARLLARLGNT2KG5KG
122623----+----++++-----------------+------------------

API biotype100

@refGLUFRUGALTREMNESBEMELSACRAFMTEMALLACLTEMbGaMaGaCELGENMbGuESCRIBARAXYLPLERHAFUCMLZDARLLARLXLTDULTAGGLYINOMANMTLTURSORADOHBGLYXERYMDG3MDGSATMUCLTATDTATMTATDMLTLMLTCATETATETTECITGRTGAT2KG5KGTRYNAGGNTPACPATpOBEQATGTEmOBEBATPPATCMTTGEBETPCEABTHINLATCAPCYTHISSUCFUMGREGYTAVTETNTTNGLNITA3OBUAPTGTTPRODALALALASERMNTPROPTYR2KT
122623++--+--+-----------+-----------+-----------------+------------+---------++-+---++---+----++---+--++

Isolation, sampling and environmental information

isolation

@refsample typecountryorigin.countrycontinentsampling dateisolation date
11538human axillar lymh nodeBelgiumBELEurope
23183clinical samples
55780Human biopsy of axillar lymph nodeBelgiumBELEurope1997
67770Human clinical sample
122623Human, Biopsy axillar lymph nodeBelgiumBELEurope1997

isolation source categories

Cat1Cat2Cat3
#Host#Human
#Host Body-Site#Limb#Arm
#Host Body-Site#Organ#Lymph node

taxonmaps

  • @ref: 69479
  • File name: preview.99_1212.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_10;96_525;97_604;98_694;99_1212&stattab=map
  • Last taxonomy: Corynebacterium
  • 16S sequence: AJ012837
  • Sequence Identity:
  • Total samples: 7374
  • soil counts: 163
  • aquatic counts: 150
  • animal counts: 6994
  • plant counts: 67

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
115382Risk group (German classification)
200572German classification
1226232Risk group (French classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Corynebacterium simulans gene for 16S rRNA, partial sequenceAB470620543ena146827
23183Corynebacterium sp. strain UCL553, 16S rRNA gene, partialAJ0128371468nuccore146827

External links

@ref: 11538

culture collection no.: DSM 44415, ATCC BAA 15, CCUG 43305, LMG 19516, JCM 12107, CIP 106488, CCM 4908, UCL 553

straininfo link

  • @ref: 72703
  • straininfo: 125705

literature

  • topic: Phylogeny
  • Pubmed-ID: 10826822
  • title: Corynebacterium simulans sp. nov., a non-lipophilic, fermentative Corynebacterium.
  • authors: Wattiau P, Janssens M, Wauters G
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/00207713-50-1-347
  • year: 2000
  • mesh: Bacterial Typing Techniques, Carbohydrate Metabolism, Corynebacterium/*classification/drug effects/genetics/*metabolism, Corynebacterium Infections/*microbiology, DNA, Bacterial/genetics, DNA, Ribosomal/genetics, Fermentation, Humans, *Lipid Metabolism, Microbial Sensitivity Tests, Molecular Sequence Data, Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA
  • topic2: Metabolism

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
11538Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 44415)https://www.dsmz.de/collection/catalogue/details/culture/DSM-44415
20057Wink, J.http://www.dsmz.de/microorganisms/wink_pdf/DSM44415.pdfCompendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
23183P Wattiau, M Janssens, G Wauters10.1099/00207713-50-1-347Corynebacterium simulans sp. nov., a non-lipophilic, fermentative Corynebacterium.IJSEM 50: 347-353 200010826822
39539Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/18624
55780Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 43305)https://www.ccug.se/strain?id=43305
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68371Automatically annotated from API 50CH acid
68379Automatically annotated from API Coryne
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
72703Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID125705.1StrainInfo: A central database for resolving microbial strain identifiers
122623Curators of the CIPCollection of Institut Pasteur (CIP 106488)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20106488