Strain identifier

BacDive ID: 3083

Type strain: Yes

Species: Corynebacterium callunae

Strain Designation: B-2244

Strain history: CIP <- 1994, R. Ruimy, CNRS, Villefranche/Mer, France <- ATCC <- NRRL: strain B-2244 <- Int. Mineral and Chemical Corp.

NCBI tax ID(s): 1121353 (strain), 1721 (species)

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General

@ref: 8571

BacDive-ID: 3083

DSM-Number: 20147

keywords: genome sequence, 16S sequence, Bacteria, obligate aerobe, mesophilic, amino acid production

description: Corynebacterium callunae B-2244 is an obligate aerobe, mesophilic bacterium that produces amino acids.

NCBI tax id

NCBI tax idMatching level
1121353strain
1721species

strain history

@refhistory
8571<- ATCC <- NRRL <- Int. Mineral and Chemical Corp
67770ATCC 15991 <-- NRRL B-2244 <-- Int. Miner. & Chem. Corp..
117107CIP <- 1994, R. Ruimy, CNRS, Villefranche/Mer, France <- ATCC <- NRRL: strain B-2244 <- Int. Mineral and Chemical Corp.

doi: 10.13145/bacdive3083.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Actinomycetes
  • order: Mycobacteriales
  • family: Corynebacteriaceae
  • genus: Corynebacterium
  • species: Corynebacterium callunae
  • full scientific name: Corynebacterium callunae (Lee and Good 1963) Yamada and Komagata 1972 (Approved Lists 1980)

  • synonyms

    • @ref: 20215
    • synonym: Corynebacterium callunae

@ref: 8571

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Actinomycetales

family: Corynebacteriaceae

genus: Corynebacterium

species: Corynebacterium callunae

full scientific name: Corynebacterium callunae (Lee and Good 1963) Yamada and Komagata 1972 emend. Nouioui et al. 2018

strain designation: B-2244

type strain: yes

Morphology

cell morphology

@refmotilityconfidencegram staincell shape
69480no94.024
69480100positive
117107positiverod-shaped

colony morphology

@refcolony colorincubation periodmedium used
18425Ivory (1014)10-14 daysISP 2
18425Light ivory (1015)10-14 daysISP 3

multicellular morphology

@refforms multicellular complexmedium name
18425noISP 2
18425noISP 3

multimedia

  • @ref: 8571
  • multimedia content: https://www.dsmz.de/microorganisms/photos/DSM_20147.jpg
  • caption: Medium 693 28°C
  • intellectual property rights: © Leibniz-Institut DSMZ

Culture and growth conditions

culture medium

@refnamegrowthcompositionlink
18425ISP 2yesName: ISP 2 / Yeast Malt Agar (5265); 5265 Composition Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l Agar 15.0 g/l Preparation: Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.0 Usage: Maintenance and Taxonomy Organisms: All Actinomycetes
18425ISP 3yesName: ISP 3; 5315 Composition Dog oat flakes 20.0 g/l Trace element solution (5314) 2.5 ml/l Agar 18.0 g/l Preparation: Oat flakes are cooked for 20 minutes, trace element solution and agar are added (in the case of non rolled oat flakes the suspension has to bee filtrated). Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.8 Usage: Maintenance and taxonomy (e.g. SEM As liquid medium for metabolite production) Organisms: All Actinomycetes Trace element solution 5314 Name: Trace element solution 5314; 5314 Composition CaCl2 x H2O 3.0 g/l Fe-III-citrate 1.0 g/l MnSO4 0.2 g/l ZnCl2 0.1 g/l CuSO4 x 5 H2O 0.025 g/l Sodium tetra borate 0.2 g/l CoCl2 x 6 H2O 0.004 g/l Sodium molybdate 0.01 g/l Preparation: Use double destillated water. Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Trace element solution for different media Organisms:
8571COLUMBIA BLOOD MEDIUM (DSMZ Medium 693)yesName: COLUMBIA BLOOD MEDIUM (DSMZ Medium 693) Composition: Defibrinated sheep blood 50.0 g/l Columbia agar basehttps://mediadive.dsmz.de/medium/693
8571TRYPTICASE SOY BROTH AGAR+BLOOD (DSMZ Medium 535a)yesName: TRYPTICASE SOY BROTH AGAR+BLOOD (DSMZ Medium 535a) Composition: None 50.0 g/l Trypticase soy broth 30.0 g/l Agar 15.0 g/l Distilled waterhttps://mediadive.dsmz.de/medium/535a
34925MEDIUM 3 - Columbia agaryesColumbia agar (39.000 g);distilled water (1000.000 ml)
8571TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535)yesName: TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535) Composition: Trypticase soy broth 30.0 g/l Agar 15.0 g/l Distilled waterhttps://mediadive.dsmz.de/medium/535
117107CIP Medium 3yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=3

culture temp

@refgrowthtypetemperaturerange
18425positiveoptimum28mesophilic
8571positivegrowth28mesophilic
34925positivegrowth30mesophilic
67770positivegrowth30mesophilic
117107positivegrowth30-41

Physiology and metabolism

oxygen tolerance

  • @ref: 117107
  • oxygen tolerance: obligate aerobe

spore formation

@refspore formationconfidence
69481no100
69480no99.994

compound production

@refcompound
8571L glutamic acid
20216L-glutamic acid

halophily

  • @ref: 18425
  • salt: NaCl
  • growth: positive
  • tested relation: maximum
  • concentration: 2.5 %

murein

  • @ref: 8571
  • murein short key: A31
  • type: A1gamma m-Dpm-direct

observation

  • @ref: 67770
  • observation: quinones: MK-9(H2)

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
1842517234glucose+
1842517992sucrose+
1842518222xylose-
1842529864mannitol-
1842528757fructose-
1842526546rhamnose-
1842516634raffinose-
1842562968cellulose-
68371Potassium 5-ketogluconate-builds acid from
68371Potassium 2-ketogluconate-builds acid from
6837118403L-arabitol-builds acid from
6837118333D-arabitol+builds acid from
6837118287L-fucose-builds acid from
6837128847D-fucose-builds acid from
6837116443D-tagatose-builds acid from
6837162318D-lyxose-builds acid from
6837132528turanose+builds acid from
6837128066gentiobiose-builds acid from
6837117151xylitol-builds acid from
6837128087glycogen-builds acid from
6837128017starch-builds acid from
6837116634raffinose-builds acid from
683716731melezitose-builds acid from
6837115443inulin-builds acid from
6837117992sucrose+builds acid from
6837128053melibiose-builds acid from
6837117716lactose-builds acid from
6837117306maltose+builds acid from
6837117057cellobiose-builds acid from
6837117814salicin+builds acid from
683714853esculin+builds acid from
6837118305arbutin+builds acid from
6837127613amygdalin-builds acid from
6837159640N-acetylglucosamine-builds acid from
68371320061methyl alpha-D-glucopyranoside-builds acid from
6837143943methyl alpha-D-mannoside-builds acid from
6837117924D-sorbitol-builds acid from
6837116899D-mannitol-builds acid from
6837117268myo-inositol-builds acid from
6837116813galactitol-builds acid from
6837162345L-rhamnose-builds acid from
6837117266L-sorbose-builds acid from
6837116024D-mannose+builds acid from
6837115824D-fructose+builds acid from
6837117634D-glucose+builds acid from
6837112936D-galactose-builds acid from
6837174863methyl beta-D-xylopyranoside-builds acid from
6837115963ribitol-builds acid from
6837165328L-xylose-builds acid from
6837165327D-xylose-builds acid from
6837116988D-ribose+builds acid from
6837130849L-arabinose-builds acid from
6837117108D-arabinose-builds acid from
6837117113erythritol-builds acid from
6837117754glycerol-builds acid from
6836917128adipate-assimilation
6836927689decanoate-assimilation
6836924265gluconate+assimilation
6836917306maltose+assimilation
6836959640N-acetylglucosamine-assimilation
6836916024D-mannose+assimilation
6836930849L-arabinose-assimilation
6836917634D-glucose+assimilation
683695291gelatin-hydrolysis
683694853esculin+hydrolysis
6836916199urea-hydrolysis
6836929016arginine-hydrolysis
6836917634D-glucose-fermentation
6836927897tryptophan-energy source
6836917632nitrate-reduction
683685291gelatin-hydrolysis
6836827897tryptophan-energy source
6836816199urea+hydrolysis
6836816947citrate-assimilation
6836818257ornithine-degradation
6836825094lysine-degradation
6836829016arginine-hydrolysis
6837917632nitrate-reduction
683794853esculin-hydrolysis
6837916199urea-hydrolysis
683795291gelatin+hydrolysis
6837917634D-glucose-fermentation
6837916988D-ribose-fermentation
6837965327D-xylose-fermentation
6837916899D-mannitol-fermentation
6837917306maltose-fermentation
6837917716lactose-fermentation
6837917992sucrose+fermentation
6837928087glycogen-fermentation

metabolite production

@refChebi-IDmetaboliteproduction
6836935581indoleno
6777029985L-glutamateyes
6836815688acetoinyes
6836835581indoleno
6836816136hydrogen sulfideno
11710735581indoleno

metabolite tests

@refChebi-IDmetaboliteindole testvoges-proskauer-test
6836935581indole-
6836815688acetoin+
6836835581indole-

enzymes

@refvalueactivityec
68382alpha-fucosidase-3.2.1.51
68382alpha-mannosidase-3.2.1.24
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382alpha-glucosidase-3.2.1.20
68382beta-glucuronidase-3.2.1.31
68382beta-galactosidase-3.2.1.23
68382alpha-galactosidase-3.2.1.22
68382alpha-chymotrypsin-3.4.21.1
68382trypsin-3.4.21.4
68382cystine arylamidase-3.4.11.3
68382leucine arylamidase+3.4.11.1
68379gelatinase+
68379urease-3.5.1.5
68379beta-glucosidase-3.2.1.21
68379N-acetyl-beta-glucosaminidase-3.2.1.52
68379alpha-glucosidase-3.2.1.20
68379beta-galactosidase+3.2.1.23
68379beta-glucuronidase+3.2.1.31
68379alkaline phosphatase+3.1.3.1
68379pyrrolidonyl arylamidase+3.4.19.3
68379pyrazinamidase+3.5.1.B15
68369gelatinase-
68369beta-glucosidase+3.2.1.21
68369urease-3.5.1.5
68369arginine dihydrolase-3.5.3.6
68368gelatinase-
68368tryptophan deaminase-4.1.99.1
68368urease+3.5.1.5
68368ornithine decarboxylase-4.1.1.17
68368lysine decarboxylase-4.1.1.18
68368arginine dihydrolase-3.5.3.6
68368beta-galactosidase-3.2.1.23
117107oxidase-
117107beta-galactosidase-3.2.1.23
117107gelatinase-
117107amylase+
117107DNase-
117107catalase+1.11.1.6
117107lecithinase-
117107urease-3.5.1.5

fatty acid profile

  • fatty acids

    @reffatty acidpercentageECL
    49383C14:02.714
    49383C16:026.616
    49383C17:0517
    49383C18:01.318
    49383C17:1 ω9c17.616.773
    49383C18:1 ω9c30.217.769
    49383C18:2 ω6,9c/C18:0 ANTE1.917.724
    49383Unidentified14.5
  • type of FA analysis: whole cell analysis
  • method/protocol: CCUG

API coryne

@refNITPYZPYRAPALbeta GURbeta GALalpha GLUbeta NAGESCUREGELGLURIBXYLMANMALLACSACGLYG
18425-+++++----+------+-

API zym

@refAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- FucosidaseControl
18425++++++----+----+---
8571-+/---+---------------
117107+++-+----++----+----

API 20E

@refONPGADH ArgLDC LysODCCITH2SURETDA TrpINDVPGEL
18425------+--+-

API 20NE

@refNO3TRPGLU_ FermADH ArgUREESCGELPNPGGLU_ AssimARAMNEMANNAGMALGNTCAPADIMLTCITPAC
8571-----+--+-++/--++--+/-++/-

API 50CHac

@refGLYERYDARALARARIBDXYLLXYLADOMDXGALGLUFRUMNESBERHADULINOMANSORMDMMDGNAGAMYARBESCSALCELMALLACMELSACTREINUMLZRAFAMDGLYGXLTGENTURLYXTAGDFUCLFUCDARLLARLGNT2KG5KG
117107----+-----+++----------+++-+--++/--------+----+-+/---

API biotype100

@refGLUFRUGALTREMNESBEMELSACRAFMTEMALLACLTEMbGaMaGaCELGENMbGuESCRIBARAXYLPLERHAFUCMLZDARLLARLXLTDULTAGGLYINOMANMTLTURSORADOHBGLYXERYMDG3MDGSATMUCLTATDTATMTATDMLTLMLTCATETATETTECITGRTGAT2KG5KGTRYNAGGNTPACPATpOBEQATGTEmOBEBATPPATCMTTGEBETPCEABTHINLATCAPCYTHISSUCFUMGREGYTAVTETNTTNGLNITA3OBUAPTGTTPRODALALALASERMNTPROPTYR2KT
117107++--+--+-++-------++------+-----------------------------+---+-++--++-----+-+--------------+------+-

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
184251German classification
85711Risk group (German classification)
1171072Risk group (French classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
8571C.callunae 16S ribosomal DNAX820531322ena1721
67770Corynebacterium callunae 16S rRNA gene, strain NCFB 10338X842511494ena1721

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Corynebacterium callunae DSM 20147GCA_000344785completencbi1121353
66792Corynebacterium callunae DSM 201471121353.12plasmidpatric1121353
66792Corynebacterium callunae DSM 201471121353.11plasmidpatric1121353
66792Corynebacterium callunae DSM 201471121353.3completepatric1121353
66792Corynebacterium callunae DSM 201471121353.8wgspatric1121353
66792Corynebacterium callunae DSM 201472561511200completeimg1121353
66792Corynebacterium callunae DSM 201472522572159draftimg1121353
67770Corynebacterium callunae DSM 20147GCA_000420585contigncbi1121353

GC content

@refGC-contentmethod
857154.0thermal denaturation, midpoint method (Tm)
857151.2
6777051.2thermal denaturation, midpoint method (Tm)
6777052.4genome sequence analysis

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingno100no
motileno93.386no
flagellatedno96.089no
gram-positiveyes91.757no
anaerobicno98.776no
aerobicyes63.047no
halophileyes59.132no
spore-formingno91.77no
glucose-utilyes88.046no
thermophileno97.85no
glucose-fermentyes51.359yes

External links

@ref: 8571

culture collection no.: CCUG 28793, CIP 104277, DSM 20147, ATCC 15991, CCM 7707, NCIB 10338, NRRL B-2244, JCM 9489, BCRC 12128, CECT 4015, HAMBI 2053, IFO 15359, IMSNU 21209, KCTC 1444, NBRC 15359, NCIMB 10338

straininfo link

  • @ref: 72601
  • straininfo: 36612

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Enzymology1359847Some properties of glutamate dehydrogenase, glutamine synthetase and glutamate synthase from Corynebacterium callunae.Ertan HArch Microbiol10.1007/BF002490631992Adenine Nucleotides/pharmacology, Amino Acids/pharmacology, Azaserine/pharmacology, Chlorides/pharmacology, Coenzymes/metabolism, Corynebacterium/*enzymology, Enzyme Activation, Enzyme Stability, Glutamate Dehydrogenase/antagonists & inhibitors/*metabolism, Glutamate Synthase/antagonists & inhibitors/*metabolism, Glutamate-Ammonia Ligase/antagonists & inhibitors/*metabolism, Glutarates/pharmacology, Hydrogen-Ion Concentration, Keto Acids/pharmacology, NADP/metabolism, Substrate Specificity, TemperatureMetabolism
Enzymology1359848The effect of various culture conditions on the levels of ammonia assimilatory enzymes of Corynebacterium callunae.Ertan HArch Microbiol10.1007/BF002490641992Ammonia/*metabolism, Corynebacterium/*enzymology/growth & development, Culture Media, Fumarates/metabolism, Glucose/metabolism, Glutamate Dehydrogenase/*analysis, Glutamate Synthase/*analysis, Glutamate-Ammonia Ligase/*analysis/antagonists & inhibitors, Glutamates/biosynthesis/metabolism, Glutamic Acid, Kinetics, Malates/metabolism, Methionine Sulfoximine/pharmacologyMetabolism
Phylogeny1713055Transfer of Brevibacterium divaricatum DSM 20297T, "Brevibacterium flavum" DSM 20411, "Brevibacterium lactofermentum" DSM 20412 and DSM 1412, and Corynebacterium glutamicum and their distinction by rRNA gene restriction patterns.Liebl W, Ehrmann M, Ludwig W, Schleifer KHInt J Syst Bacteriol10.1099/00207713-41-2-2551991Base Composition, Base Sequence, Brevibacterium/*classification/genetics, Corynebacterium/*classification/genetics, DNA, Bacterial/genetics, DNA, Ribosomal/genetics, Molecular Sequence Data, Nucleic Acid Hybridization, *Polymorphism, Restriction Fragment Length, RNA, Bacterial/genetics, RNA, Ribosomal, 16S/*genetics, RNA, Ribosomal, 23S/*geneticsGenetics
Genetics26203323Genome sequence of the soil bacterium Corynebacterium callunae type strain DSM 20147(T).Persicke M, Albersmeier A, Bednarz H, Niehaus K, Kalinowski J, Ruckert CStand Genomic Sci10.1186/1944-3277-10-52015
Phylogeny27666312Corynebacterium crudilactis sp. nov., isolated from raw cow's milk.Zimmermann J, Ruckert C, Kalinowski J, Lipski AInt J Syst Evol Microbiol10.1099/ijsem.0.0015092016Animals, Bacterial Typing Techniques, Base Composition, Cattle, Corynebacterium/*classification/genetics/isolation & purification, DNA, Bacterial/genetics, Fatty Acids/chemistry, Genes, Bacterial, Germany, Milk/*microbiology, Nucleic Acid Hybridization, Peptidoglycan/chemistry, Phospholipids/chemistry, Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Vitamin K 2/chemistryTranscriptome
Genetics28249784Complete genome sequencing of newly isolated thermotolerant Corynebacterium glutamicum N24 provides a new insights into its thermotolerant phenotype.Matsutani M, Nantapong N, Murata R, Paisrisan P, Hirakawa H, Kataoka N, Yakushi T, Matsushita KJ Biotechnol10.1016/j.jbiotec.2017.02.0252017Bacterial Proteins/*genetics, Base Sequence, Chromosome Mapping, Conserved Sequence, Corynebacterium glutamicum/genetics/*isolation & purification, Genome Size, Genome, Bacterial, Phylogeny, Sequence Analysis, DNA/*methods, Soil Microbiology, *ThermotolerancePhylogeny
Phylogeny28429167Corynebacterium defluvii sp. nov., isolated from Sewage.Yu QL, Yan ZF, He X, Tian FH, Jia CW, Li CTJ Microbiol10.1007/s12275-017-6592-32017Base Composition, China, *Corynebacterium/classification/genetics/isolation & purification, DNA, Bacterial/genetics, Fatty Acids/analysis, Peptidoglycan/analysis, Phospholipids/analysis, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Sewage/*microbiologyTranscriptome
Phylogeny30688628Corynebacterium alimapuense sp. nov., an obligate marine actinomycete isolated from sediment of Valparaiso bay, Chile.Claverias F, Gonzales-Siles L, Salva-Serra F, Inganas E, Molin K, Cumsille A, Undabarrena A, Couve E, Moore ERB, Tindall BJ, Gomila M, Camara BInt J Syst Evol Microbiol10.1099/ijsem.0.0032372019Bacterial Typing Techniques, Base Composition, Bays, Chile, Corynebacterium/*classification/isolation & purification, DNA, Bacterial/genetics, Fatty Acids/chemistry, Geologic Sediments/*microbiology, Glycolipids/chemistry, Nucleic Acid Hybridization, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Seawater/*microbiology, Sequence Analysis, DNA, Vitamin K 2/analogs & derivatives/chemistryTranscriptome

Reference

@idauthorscataloguedoi/urltitle
8571Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 20147)https://www.dsmz.de/collection/catalogue/details/culture/DSM-20147
18425Wink, J.http://www.dsmz.de/microorganisms/wink_pdf/DSM20147.pdfCompendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20216Curators of the HKIhttp://www.leibniz-hki.de/de/Collection Description Leibniz-Institut für Naturstoff-Forschung und Infektionsbiologie e. V. Hans-Knöll-Institut (HKI)
34925Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/16168
49383Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 28793)https://www.ccug.se/strain?id=28793
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68368Automatically annotated from API 20E
68369Automatically annotated from API 20NE
68371Automatically annotated from API 50CH acid
68379Automatically annotated from API Coryne
68382Automatically annotated from API zym
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch10.21203/rs.3.rs-2527258/v1Predictions based on the model GenomeNet Sporulation v. 1
72601Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID36612.1StrainInfo: A central database for resolving microbial strain identifiers
117107Curators of the CIPCollection of Institut Pasteur (CIP 104277)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20104277