Strain identifier

BacDive ID: 3072

Type strain: Yes

Species: Adlercreutzia equolifaciens subsp. celata

Strain Designation: do03

Strain history: K. Asano do03 <-- K. Minamida do03.

NCBI tax ID(s): 1121021 (strain), 394340 (subspecies)

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General

@ref: 7696

BacDive-ID: 3072

DSM-Number: 18785

keywords: genome sequence, 16S sequence, Bacteria, anaerobe, mesophilic, Gram-positive, rod-shaped

description: Adlercreutzia equolifaciens subsp. celata do03 is an anaerobe, mesophilic, Gram-positive bacterium that was isolated from rat caecum.

NCBI tax id

NCBI tax idMatching level
1121021strain
394340subspecies

strain history

@refhistory
7696<- K. Asano <-K. Minamida, strain do03
67770K. Asano do03 <-- K. Minamida do03.

doi: 10.13145/bacdive3072.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Coriobacteriia
  • order: Eggerthellales
  • family: Eggerthellaceae
  • genus: Adlercreutzia
  • species: Adlercreutzia equolifaciens subsp. celata
  • full scientific name: Adlercreutzia equolifaciens subsp. celata corrig. (Minamida et al. 2008) Nouioui et al. 2018

  • synonyms

    @refsynonym
    20215Asaccharobacter celatus
    20215Adlercreutzia equolifaciens subsp. celatus

@ref: 7696

domain: Bacteria

phylum: Actinobacteria

class: Coriobacteriia

order: Coriobacteriales

family: Eggerthellaceae

genus: Adlercreutzia

species: Adlercreutzia equolifaciens subsp. celatus

full scientific name: Adlercreutzia equolifaciens subsp. celatus (Minamida et al. 2008) Nouioui et al. 2018

strain designation: do03

type strain: yes

Morphology

cell morphology

@refgram staincell lengthcell widthcell shapemotilityconfidence
32059positive2.5 µm0.45 µmrod-shaped
69480no92.665
69480positive99.741

pigmentation

  • @ref: 32059
  • production: no

Culture and growth conditions

culture medium

  • @ref: 7696
  • name: CHOPPED MEAT MEDIUM WITH CARBOHYDRATES (DSMZ Medium 110)
  • growth: yes
  • link: https://mediadive.dsmz.de/medium/110
  • composition: Name: CHOPPED MEAT MEDIUM WITH CARBOHYDRATES (DSMZ Medium 110) Composition: Ground beef 500.0 g/l Casitone 30.0 g/l Agar 15.0 g/l K2HPO4 5.0 g/l Yeast extract 5.0 g/l D-Glucose 4.0 g/l Starch 1.0 g/l Maltose 1.0 g/l Cellobiose 1.0 g/l L-Cysteine HCl 0.5 g/l Ethanol 0.19 g/l Vitamin K3 0.05 g/l Hemin 0.005 g/l Sodium resazurin 0.0005 g/l Vitamin K1 NaOH Distilled water

culture temp

@refgrowthtypetemperaturerange
7696positivegrowth37mesophilic
32059positivegrowth37mesophilic
67770positivegrowth37mesophilic

Physiology and metabolism

oxygen tolerance

@refoxygen toleranceconfidence
7696anaerobe
32059anaerobe
69480anaerobe99.999

spore formation

@refspore formationconfidence
32059no
69481no100
69480no99.977

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
6838016199urea-hydrolysis
6838029016arginine+hydrolysis
6838016024D-mannose-fermentation
6838016634raffinose-fermentation
6838029985L-glutamate-degradation
6838017632nitrate-reduction
6838027897tryptophan-energy source

metabolite production

  • @ref: 68380
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

metabolite tests

  • @ref: 68380
  • Chebi-ID: 35581
  • metabolite: indole
  • indole test: -

enzymes

@refvalueactivityec
32059acid phosphatase+3.1.3.2
32059alkaline phosphatase+3.1.3.1
68380serine arylamidase-
68380glutamyl-glutamate arylamidase-
68380histidine arylamidase-
68380glycin arylamidase-
68380alanine arylamidase-3.4.11.2
68380tyrosine arylamidase-
68380pyrrolidonyl arylamidase-3.4.19.3
68380leucine arylamidase-3.4.11.1
68380phenylalanine arylamidase-
68380leucyl glycin arylamidase-3.4.11.1
68380proline-arylamidase-3.4.11.5
68380L-arginine arylamidase+
68380alkaline phosphatase-3.1.3.1
68380tryptophan deaminase-4.1.99.1
68380alpha-fucosidase-3.2.1.51
68380glutamate decarboxylase-4.1.1.15
68380N-acetyl-beta-glucosaminidase-3.2.1.52
68380beta-glucuronidase-3.2.1.31
68380alpha-arabinosidase-3.2.1.55
68380beta-glucosidase-3.2.1.21
68380alpha-glucosidase-3.2.1.20
68380beta-Galactosidase 6-phosphate-
68380beta-galactosidase-3.2.1.23
68380alpha-galactosidase-3.2.1.22
68380arginine dihydrolase+3.5.3.6
68380urease-3.5.1.5

API rID32A

@refUREADH Argalpha GALbeta GALbeta GPalpha GLUbeta GLUalpha ARAbeta GURbeta NAGMNERAFGDCalpha FUCNITINDPALArgAProALGAPheALeuAPyrATyrAAlaAGlyAHisAGGASerA
7696-+---------------+-----------

Isolation, sampling and environmental information

isolation

@refsample typecountryorigin.countrycontinent
7696rat caecumJapanJPNAsia
67770Rat cecum

isolation source categories

Cat1Cat2Cat3
#Host#Mammals#Muridae (Mouse/Rat)
#Host Body-Site#Gastrointestinal tract

taxonmaps

  • @ref: 69479
  • File name: preview.99_4556.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_15800;96_2274;97_2756;98_3424;99_4556&stattab=map
  • Last taxonomy: Adlercreutzia equolifaciens subclade
  • 16S sequence: AB849124
  • Sequence Identity:
  • Total samples: 103294
  • soil counts: 562
  • aquatic counts: 2542
  • animal counts: 99975
  • plant counts: 215

Safety information

risk assessment

  • @ref: 7696
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Asaccharobacter celatus gene for 16S ribosomal RNA, partial sequence, strain: JCM 14811AB8491241469ena394340
7696Asaccharobacter celatus gene for 16S rRNA, partial sequenceAB2661021428ena1121021

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Adlercreutzia equolifaciens subsp. celatus JCM 14811GCA_016865385completencbi394340
66792Adlercreutzia equolifaciens subsp. celatus DSM 18785GCA_003726015contigncbi1121021
66792Asaccharobacter celatus DSM 187851121021.3wgspatric1121021
66792Asaccharobacter celatus DSM 187851121021.16completepatric1121021
66792Asaccharobacter celatus strain JCM 14811394340.5wgspatric394340
66792Asaccharobacter celatus DSM 187852574179708draftimg1121021
67770Adlercreutzia equolifaciens subsp. celatus JCM 14811GCA_003428485contigncbi394340
66792Adlercreutzia equolifaciens subsp. celatus DSM 18785GCA_024171685contigncbi1121021

GC content

@refGC-contentmethod
769663
6777063high performance liquid chromatography (HPLC)

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingno100no
motileno94.609no
flagellatedno98.845no
gram-positiveyes92.33no
anaerobicyes99.037no
aerobicno95.091yes
halophileno87.412no
spore-formingno92.259no
thermophileno99.205yes
glucose-utilyes64.094no
glucose-fermentno59.417no

External links

@ref: 7696

culture collection no.: DSM 18785, JCM 14811, AHU 1763, KCTC 15587

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny18450720Asaccharobacter celatus gen. nov., sp. nov., isolated from rat caecum.Minamida K, Ota K, Nishimukai M, Tanaka M, Abe A, Sone T, Tomita F, Hara H, Asano KInt J Syst Evol Microbiol10.1099/ijs.0.64894-02008Animals, Bacteria, Anaerobic/*classification/genetics/isolation & purification/physiology, Bacterial Typing Techniques, Base Composition, Cecum/*microbiology, DNA, Bacterial/analysis, DNA, Ribosomal/analysis, Genes, rRNA, Genotype, Gram-Positive Rods/*classification/genetics/isolation & purification/physiology, Molecular Sequence Data, Phenotype, Phylogeny, RNA, Ribosomal, 16S/genetics, Rats, Sequence Analysis, DNA, Species SpecificityGenetics
Enzymology19502755Biotransformation of daidzein to equol by crude enzyme from Asaccharobacter celatus AHU1763 required an anaerobic environment.Thawornkuno C, Tanaka M, Sone T, Asano KBiosci Biotechnol Biochem10.1271/bbb.809082009Anaerobiosis, Biotransformation, Equol, Gram-Positive Bacteria/*enzymology/growth & development, Hydrogen-Ion Concentration, Isoflavones/*pharmacokinetics, Temperature
Phylogeny19542111Isolation of bacteria from the ileal mucosa of TNFdeltaARE mice and description of Enterorhabdus mucosicola gen. nov., sp. nov.Clavel T, Charrier C, Braune A, Wenning M, Blaut M, Haller DInt J Syst Evol Microbiol10.1099/ijs.0.003087-02009Actinobacteria/*classification/genetics/*isolation & purification/physiology, Animals, Bacterial Typing Techniques, Base Composition, DNA, Bacterial/analysis, Fatty Acids/analysis, Female, Genes, rRNA, Genotype, Ileum/*microbiology, Intestinal Mucosa/*microbiology, Mice, Mice, Inbred C57BL, Mice, Mutant Strains, Molecular Sequence Data, Phenotype, Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Species Specificity, Tumor Necrosis Factor-alpha/*geneticsGenetics
Metabolism25747993Biotransformation of (-)-epigallocatechin and (-)-gallocatechin by intestinal bacteria involved in isoflavone metabolism.Takagaki A, Nanjo FBiol Pharm Bull10.1248/bpb.b14-006462015Actinobacteria/*metabolism, Biotransformation, Catechin/*analogs & derivatives/metabolism, Intestines/microbiology, Isoflavones/metabolism
Phylogeny29583112Ellagibacter isourolithinifaciens gen. nov., sp. nov., a new member of the family Eggerthellaceae, isolated from human gut.Beltran D, Romo-Vaquero M, Espin JC, Tomas-Barberan FA, Selma MVInt J Syst Evol Microbiol10.1099/ijsem.0.0027352018Actinobacteria/*classification/genetics/isolation & purification, Adult, Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Diaminopimelic Acid, Fatty Acids/chemistry, Feces/microbiology, Gastrointestinal Tract/*microbiology, Glycolipids/chemistry, Humans, Male, Peptidoglycan/chemistry, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Vitamin K 2/analogs & derivatives/chemistryPathogenicity
Genetics33986097Complete Genome Sequence of Adlercreutzia equolifaciens subsp. celatus DSM 18785.Takahashi H, Yang J, Yamamoto H, Fukuda S, Arakawa KMicrobiol Resour Announc10.1128/MRA.00354-212021

Reference

@idauthorscataloguedoi/urltitlejournalpubmedID_cross_reference
7696Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 18785)https://www.dsmz.de/collection/catalogue/details/culture/DSM-18785
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
32059Barberan A, Caceres Velazquez H, Jones S, Fierer N.10.1128/mSphere.00237-17Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information2877604128308
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68380Automatically annotated from API rID32A
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch10.21203/rs.3.rs-2527258/v1Predictions based on the model GenomeNet Sporulation v. 1