Strain identifier

BacDive ID: 3033

Type strain: Yes

Species: Limnohabitans australis

Strain Designation: MWH-BRAZ-DAM2D

Strain history: <- M. W. Hahn, Austrian Academy of Sciences, Inst. Limnology; MWH-BRAZ-DAM2D

NCBI tax ID(s): 544681 (species)

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General

@ref: 15864

BacDive-ID: 3033

DSM-Number: 21646

keywords: 16S sequence, Bacteria, aerobe, chemoorganotroph, mesophilic, rod-shaped, colony-forming

description: Limnohabitans australis MWH-BRAZ-DAM2D is an aerobe, chemoorganotroph, mesophilic bacterium that forms circular colonies and was isolated from subtropical freshwater pond.

NCBI tax id

  • NCBI tax id: 544681
  • Matching level: species

strain history

  • @ref: 15864
  • history: <- M. W. Hahn, Austrian Academy of Sciences, Inst. Limnology; MWH-BRAZ-DAM2D

doi: 10.13145/bacdive3033.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Betaproteobacteria
  • order: Burkholderiales
  • family: Comamonadaceae
  • genus: Limnohabitans
  • species: Limnohabitans australis
  • full scientific name: Limnohabitans australis Hahn et al. 2010

@ref: 15864

domain: Bacteria

phylum: Proteobacteria

class: Betaproteobacteria

order: Burkholderiales

family: Comamonadaceae

genus: Limnohabitans

species: Limnohabitans australis

full scientific name: Limnohabitans australis Hahn et al. 2010

strain designation: MWH-BRAZ-DAM2D

type strain: yes

Morphology

cell morphology

  • @ref: 23096
  • cell length: 1.0-1.7 µm
  • cell width: 0.4-0.5 µm
  • cell shape: rod-shaped
  • motility: no

colony morphology

@refcolony colorcolony shapemedium usedincubation period
23096unpigmentedcircularnutrient broth soytone yeast extract medium
610673-5 days

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
15864R2A MEDIUM (DSMZ Medium 830)yeshttps://mediadive.dsmz.de/medium/830Name: R2A MEDIUM (DSMZ Medium 830) Composition: Agar 15.0 g/l Casamino acids 0.5 g/l Starch 0.5 g/l Glucose 0.5 g/l Proteose peptone 0.5 g/l Yeast extract 0.5 g/l K2HPO4 0.3 g/l Na-pyruvate 0.3 g/l MgSO4 x 7 H2O 0.05 g/l Distilled water
23096Casitone agar (Difco BD)yes
23096Luria-Bertani agar (Difco BD)yes
23096nutrient broth soytone yeast extract mediumyes
23096R2A agar (Remel)yes

culture temp

@refgrowthtypetemperaturerange
15864positivegrowth28mesophilic
23096positivegrowth12.0-36.0
61067positivegrowth30mesophilic

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
23096aerobe
23096facultative anaerobe
61067aerobe

nutrition type

  • @ref: 23096
  • type: chemoorganotroph

halophily

  • @ref: 23096
  • salt: NaCl
  • growth: positive
  • tested relation: growth
  • concentration: 0.0-0.2 %(w/v)

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
2309616947citrate-assimilation
2309612936D-galactose-assimilation
2309616024D-mannose-assimilation
2309616988D-ribose-assimilation
2309625115malate-assimilation
2309616236ethanol-assimilation
2309617754glycerol-assimilation
2309629805glycolate-assimilation
2309629985L-glutamate-assimilation
2309618050L-glutamine-assimilation
2309615971L-histidine-assimilation
2309617295L-phenylalanine-assimilation
2309617203L-proline-assimilation
2309617115L-serine-assimilation
2309616828L-tryptophan-assimilation
2309616452oxaloacetate-assimilation
2309617272propionate-assimilation
2309617992sucrose-assimilation
23096309162-oxoglutarate+assimilation
2309630089acetate+assimilation
2309633871glycerate+assimilation
2309629806fumarate+assimilation
2309624265gluconate+assimilation
2309617234glucose+assimilation
2309636655glyoxylate+assimilation
2309615792malonate+assimilation
2309615361pyruvate+assimilation
2309630031succinate+assimilation

enzymes

@refvalueactivityec
23096catalase-1.11.1.6
23096cytochrome oxidase+1.9.3.1

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinentlatitudelongitudeenrichment cultureisolation proceduresampling date
15864subtropical freshwater pondMonjolinho pond, campus of the Federal Univ. São Carlos, 21° 59' 10.14'' S 47° 52' 50.28'' WBrazilBRAMiddle and South America-21.9861-47.8806
23096nutrient broth soytone yeast extract mediumdilution-acclimatization method
61067Freshwater,pondSao Carlos,Monjolinho PondBrazilBRAMiddle and South America2008-03-08

isolation source categories

Cat1Cat2Cat3
#Environmental#Aquatic#Freshwater
#Environmental#Aquatic#Pond (small)
#Climate#Temperate#Subtropical

taxonmaps

  • @ref: 69479
  • File name: preview.99_62502.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_1;96_269;97_2286;98_9808;99_62502&stattab=map
  • Last taxonomy: Comamonadaceae
  • 16S sequence: FM178226
  • Sequence Identity:
  • Total samples: 1853
  • soil counts: 44
  • aquatic counts: 1253
  • animal counts: 163
  • plant counts: 393

Safety information

risk assessment

  • @ref: 15864
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

  • @ref: 15864
  • description: Limnohabitans australis 16S rRNA gene (partial), tRNA-Ile, tRNA-Ala and 23S rRNA (partial) genes, type strain MWH-BRAZ-DAM2DT
  • accession: FM178226
  • length: 2194
  • database: ena
  • NCBI tax ID: 544681

GC content

@refGC-contentmethod
1586455.8high performance liquid chromatography (HPLC)
2309655.8

External links

@ref: 15864

culture collection no.: CCUG 57769, DSM 21646, CCUG 56719, KCTC 42566

straininfo link

  • @ref: 72553
  • straininfo: 405440

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny20118294Limnohabitans australis sp. nov., isolated from a freshwater pond, and emended description of the genus Limnohabitans.Hahn MW, Kasalicky V, Jezbera J, Brandt U, Simek KInt J Syst Evol Microbiol10.1099/ijs.0.022384-02010Bacterial Typing Techniques, Base Composition, Brazil, Comamonadaceae/*classification/genetics/isolation & purification, DNA, Bacterial/genetics, Fatty Acids/chemistry, Fresh Water/microbiology, Molecular Sequence Data, Nucleic Acid Hybridization, *Phylogeny, Ponds/*microbiology, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Ubiquinone/chemistryGenetics
Phylogeny34402776Limnohabitans radicicola sp. nov., a slow-growing bacterium isolated from rhizosphere of rice plant and emended description of the genus Limnohabitans.Chhetri G, Kim J, Kim I, Kang M, Seo TInt J Syst Evol Microbiol10.1099/ijsem.0.0049572021Bacterial Typing Techniques, Base Composition, Comamonadaceae/*classification/isolation & purification, DNA, Bacterial/genetics, Fatty Acids/chemistry, Nucleic Acid Hybridization, *Oryza, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, *Rhizosphere, Sequence Analysis, DNA, *Soil Microbiology, Ubiquinone/chemistryTranscriptome

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
15864Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 21646)https://www.dsmz.de/collection/catalogue/details/culture/DSM-21646
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
23096Martin W. Hahn,Vojtech Kasalický,Jan Jezbera,Ulrike Brandt,Karel Siimek10.1099/ijs.0.022384-0Limnohabitans australis sp. nov., isolated from a freshwater pond, and emended description of the genus LimnohabitansIJSEM 60: 2946-2950 201020118294
61067Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 56719)https://www.ccug.se/strain?id=56719
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
72553Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID405440.1StrainInfo: A central database for resolving microbial strain identifiers