Strain identifier

BacDive ID: 2923

Type strain: Yes

Species: Comamonas testosteroni

Strain history: CIP <- 1959, NCIB <- 1958, ATCC <- P. Talalay, Pseudomonas testosteroni

NCBI tax ID(s): 1009852 (strain), 285 (species)

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For citation purpose refer to the digital object identifier (doi) of the current version.
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General

@ref: 12682

BacDive-ID: 2923

DSM-Number: 50244

keywords: genome sequence, 16S sequence, Bacteria, mesophilic, motile, human pathogen

description: Comamonas testosteroni DSM 50244 is a mesophilic, motile human pathogen that was isolated from soil, enrichment with testosterone.

NCBI tax id

NCBI tax idMatching level
285species
1009852strain

strain history

@refhistory
12682<- ICPB <- P. Talalay
67770Y. Kosako 85013 <-- Nissui Pharm. Co., Ltd. <-- ATCC 11996 <-- P. Talalay.
123485CIP <- 1959, NCIB <- 1958, ATCC <- P. Talalay, Pseudomonas testosteroni

doi: 10.13145/bacdive2923.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Betaproteobacteria
  • order: Burkholderiales
  • family: Comamonadaceae
  • genus: Comamonas
  • species: Comamonas testosteroni
  • full scientific name: Comamonas testosteroni (Marcus and Talalay 1956) Tamaoka et al. 1987

  • synonyms

    • @ref: 20215
    • synonym: Pseudomonas testosteroni

@ref: 12682

domain: Bacteria

phylum: Proteobacteria

class: Betaproteobacteria

order: Burkholderiales

family: Comamonadaceae

genus: Comamonas

species: Comamonas testosteroni

full scientific name: Comamonas testosteroni (Marcus and Talalay 1956) Tamaoka et al. 1987 emend. Willems et al. 1991

type strain: yes

Morphology

cell morphology

@refmotilityconfidencegram stain
69480yes96.209
69480100negative

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
12682NUTRIENT AGAR (DSMZ Medium 1)yeshttps://mediadive.dsmz.de/medium/1Name: NUTRIENT AGAR (DSMZ Medium 1) Composition: Agar 15.0 g/l Peptone 5.0 g/l Meat extract 3.0 g/l Distilled water
37573MEDIUM 3 - Columbia agaryesColumbia agar (39.000 g);distilled water (1000.000 ml)
123485CIP Medium 3yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=3

culture temp

@refgrowthtypetemperaturerange
12682positivegrowth30mesophilic
37573positivegrowth30mesophilic
67770positivegrowth30mesophilic

Physiology and metabolism

spore formation

@refspore formationconfidence
69481no100
69480no99.996

compound production

@refcompound
12682ß lactamase
126829-alpha hydroxysteroid dehydrogenase
12682ß hydroxysteroid dehydrogenase

observation

  • @ref: 67770
  • observation: quinones: Q-8

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
6836925115malate+assimilation
6836917128adipate+assimilation
6836927689decanoate+assimilation
6836924265gluconate+assimilation
6836917306maltose-assimilation
6836959640N-acetylglucosamine-assimilation
6836916899D-mannitol-assimilation
6836916024D-mannose-assimilation
6836930849L-arabinose-assimilation
6836917634D-glucose-assimilation
683695291gelatin-hydrolysis
683694853esculin-hydrolysis
6836916199urea-hydrolysis
6836929016arginine-hydrolysis
6836917634D-glucose-fermentation
6836927897tryptophan-energy source
6836917632nitrate+reduction

metabolite production

  • @ref: 68369
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

metabolite tests

  • @ref: 68369
  • Chebi-ID: 35581
  • metabolite: indole
  • indole test: -

enzymes

@refvalueactivityec
68369cytochrome oxidase+1.9.3.1
68369gelatinase-
68369beta-glucosidase-3.2.1.21
68369urease-3.5.1.5
68369arginine dihydrolase-3.5.3.6

API 20NE

@refNO3TRPGLU_ FermADH ArgUREESCGELPNPGGLU_ AssimARAMNEMANNAGMALGNTCAPADIMLTCITPACOX
12682+-------------+++++-+
12682+-------------+++++-+

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinent
12682soil, enrichment with testosteroneBerkeleyUSAUSANorth America
44373Soil,enrichment with testosterone
67770Soil
123485Environment, SoilBerkeley, CaliforniaUnited States of AmericaUSANorth America

isolation source categories

  • Cat1: #Environmental
  • Cat2: #Terrestrial
  • Cat3: #Soil

taxonmaps

  • @ref: 69479
  • File name: preview.99_294.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_1;96_202;97_220;98_249;99_294&stattab=map
  • Last taxonomy: Comamonas
  • 16S sequence: EU024141
  • Sequence Identity:
  • Total samples: 45208
  • soil counts: 5571
  • aquatic counts: 17183
  • animal counts: 18603
  • plant counts: 3851

Safety information

risk assessment

@refpathogenicity humanbiosafety levelbiosafety level comment
12682yes, in single cases1Risk group (German classification)
1234851Risk group (French classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Pseudomonas testosteroni 16S rRNA geneM112241536ena285
20218Comamonas testosteroni strain BCRC 14822 16S-23S ribosomal RNA intergenic spacer, complete sequenceEU014531759ena285
20218Comamonas testosteroni strain LMG 1800 16S ribosomal RNA gene, partial sequenceEU0241411317ena1009852
20218Comamonas testosteroni gene for 16S rRNA, partial sequence, strain: NBRC 14951AB6807201458ena285

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Comamonas testosteroni ATCC 11996 4GCA_903797675scaffoldncbi1009852
66792Comamonas testosteroni ATCC 119961009852.3wgspatric1009852
66792Comamonas testosteroni ATCC 11996 strain 41009852.10wgspatric1009852
66792Comamonas testosteroni strain NBRC 14951285.47wgspatric285
66792Comamonas testosteroni strain NCTC10698285.64wgspatric285
66792Comamonas testosteroni NBRC 149512617271275draftimg285
66792Comamonas testosteroni ATCC 119962529292992draftimg1009852
67770Comamonas testosteroni ATCC 11996GCA_000241525contigncbi1009852
67770Comamonas testosteroni NBRC 14951GCA_000740035contigncbi285
67770Comamonas testosteroni NCTC10698GCA_900461225contigncbi285

GC content

@refGC-contentmethod
1268262.5
6777061.5high performance liquid chromatography (HPLC)

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingno100no
flagellatedyes80.104no
gram-positiveno98.485no
anaerobicno98.015no
aerobicyes93.699no
halophileno95.1no
spore-formingno95.12no
thermophileno99.088no
glucose-utilno82.281yes
motileyes88.769no
glucose-fermentno90.675no

External links

@ref: 12682

culture collection no.: DSM 50244, ATCC 11996, ICPB 2741-78, NCIB 8955, CCUG 1426, NRRL B-2611, NCTC 10698, LMG 1786, JCM 5832, BCRC 11023, BCRC 14822, CCM 1931, CECT 326, CIP 59.24, HAMBI 403, IAM 12419, IFO 14951, JCM 6221, KCTC 1772, LMG 1800, NBRC 14951, NCAIM B.01655, NCAIM B.01926, NCIMB 8955, NCPPB 1969, VKM B-1241, VTT E-86249, NRRL: B2611

straininfo link

  • @ref: 72446
  • straininfo: 2054

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Enzymology181083Affinity chromatography of 3 alpha-hydroxysteroid dehydrogenase from Pseudomonas testosteroni. Use of N,N-dimethylformamide to prevent hydrophobic interactions between the enzyme and the ligand.Aukrust LE, Norum KR, Skalhegg BABiochim Biophys Acta10.1016/0005-2744(76)90219-91976Chromatography, Affinity, Dimethylformamide/*pharmacology, Ethylenediamines, Glycocholic Acid, Hydroxysteroid Dehydrogenases/isolation & purification/*metabolism, NAD/pharmacology, Protein Binding, Pseudomonas/*enzymology, SepharosePhylogeny
Enzymology2348463Alpha-hydroxysteroid dehydrogenase from Pseudomonas testosteroni: kinetic properties with NAD and its thionicotinamide analogue.Skalhegg BAEur J Biochem10.1111/j.1432-1033.1975.tb09901.x1975Androstanes, Androsterone, Cholic Acids, Chromatography, Gel, Deoxycholic Acid, Etiocholanolone, Hydroxysteroid Dehydrogenases/antagonists & inhibitors/*metabolism, Kinetics, NAD/analogs & derivatives/*metabolism, NADP, Pregnanediol, Pseudomonas/*enzymology, Structure-Activity Relationship, Tetrahydrocortisol, TetrahydrocortisoneMetabolism
Enzymology238957Purification and properties of malate dehydrogenase from Pseudomonas testosteroni.You KS, Kaplan NOJ Bacteriol10.1128/jb.123.2.704-716.19751975Amino Acids/analysis, Bacterial Proteins/analysis, Depression, Chemical, Enzyme Activation, Hydrogen-Ion Concentration, Kinetics, Malate Dehydrogenase/isolation & purification/*metabolism, Malates/metabolism, Malonates/metabolism/pharmacology, Molecular Weight, Protein Conformation, Pseudomonas/*enzymology, Spectrometry, Fluorescence, Stereoisomerism, Structure-Activity Relationship, Tartrates/metabolism/pharmacologyMetabolism
Enzymology1164792Specificity of oxidation of bile-salt hydroxyl groups by crude extracts of Pseudomonas testosteroni (ATCC 11996) used in determining bile salts.Beher WT, Lin GJClin Chem1975Bile Acids and Salts/*analysis, Hydroxysteroid Dehydrogenases/*metabolism, Methods, Pseudomonas/*enzymologyMetabolism
Enzymology1184284On the 3alpha-hydroxysteroid dehydrogenase from Pseudomonas testosteroni. The effect of denaturing agents.Skalhegg BAInt J Pept Protein Res10.1111/j.1399-3011.1975.tb02449.x1975Androsterone/metabolism, Chromatography, Thin Layer, Deoxycholic Acid/metabolism, Electrophoresis, Cellulose Acetate, Electrophoresis, Polyacrylamide Gel, Hydroxysteroid Dehydrogenases/*analysis/metabolism, Isoenzymes/analysis/metabolism, Molecular Weight, Peptides/analysis, Protein Denaturation, Pseudomonas/*enzymology, Tetrahydrocortisone/metabolism, UltracentrifugationMetabolism
Metabolism1369058A novel sulfatase from Pseudomonas testosteroni hydrolyzing lithocholic acid sulfate.Tazuke Y, Matsuda K, Okada S, Tsukada YBiosci Biotechnol Biochem10.1271/bbb.56.15841992Chromatography, Gas, Culture Media, Enzyme Induction, Hydrolysis, Lithocholic Acid/*analogs & derivatives/metabolism, Manganese/pharmacology, Pseudomonas/*enzymology, Sulfatases/biosynthesis/isolation & purification/*metabolism, Sulfates/pharmacology, Sulfuric Acids/metabolismCultivation
Metabolism8809204Antibiotic resistance and enhanced insecticide catabolism as consequences of steroid induction in the gram-negative bacterium Comamonas testosteroni.Oppermann UC, Belai I, Maser EJ Steroid Biochem Mol Biol10.1016/0960-0760(96)00021-01996Anti-Bacterial Agents/pharmacology, *Drug Resistance, Microbial, Fusidic Acid/pharmacology, Gram-Negative Aerobic Bacteria/*drug effects/*metabolism, Insecticides/*metabolism/*pharmacokinetics, Metyrapone/analogs & derivatives/metabolism/pharmacology, Steroids/*metabolism, Testosterone/metabolismPathogenicity
Enzymology8944761Characterization of a 3 alpha-hydroxysteroid dehydrogenase/carbonyl reductase from the gram-negative bacterium Comamonas testosteroni.Oppermann UC, Maser EEur J Biochem10.1111/j.1432-1033.1996.00744.x19963-Hydroxysteroid Dehydrogenases/biosynthesis/immunology/isolation & purification/*metabolism, 3-alpha-Hydroxysteroid Dehydrogenase (B-Specific), Alcohol Oxidoreductases/isolation & purification/metabolism, Aldehyde Reductase, Aldo-Keto Reductases, Amino Acid Sequence, Cross Reactions, Enzyme Induction, Gram-Negative Aerobic Bacteria/drug effects/*enzymology, Molecular Sequence Data, Pseudomonas/enzymology, Sequence Analysis, Subcellular Fractions/enzymology, Substrate Specificity, Testosterone/pharmacology, Xenobiotics/metabolismMetabolism
Metabolism9805374Purification and properties of a novel sulfatase from Pseudomonas testosteroni that hydrolyzed 3 beta-hydroxy-5-cholenoic acid 3-sulfate.Tazuke Y, Matsuda K, Adachi K, Tsukada YBiosci Biotechnol Biochem10.1271/bbb.62.17391998Arylsulfatases/*metabolism, Cholic Acids/metabolism, Hydrogen-Ion Concentration, Hydrolysis, Ions, Isoelectric Point, Molecular Weight, Pseudomonas/*enzymology, Sulfates/metabolism, TemperatureEnzymology
Metabolism15474469Steroid degradation gene cluster of Comamonas testosteroni consisting of 18 putative genes from meta-cleavage enzyme gene tesB to regulator gene tesR.Horinouchi M, Kurita T, Yamamoto T, Hatori E, Hayashi T, Kudo TBiochem Biophys Res Commun10.1016/j.bbrc.2004.09.0962004Bacterial Proteins/*genetics, Blotting, Northern, Chromatography, High Pressure Liquid, Cloning, Molecular, Comamonas testosteroni/*genetics, DNA Transposable Elements, Escherichia coli/metabolism, *Genes, Bacterial, Models, Chemical, Models, Genetic, *Multigene Family, Mutagenesis, Mutation, Open Reading Frames, Oxidoreductases/*genetics, Plasmids/metabolism, Steroids/*metabolism, Temperature, Testosterone/metabolism, Time Factors, Transcription Factors/*genetics, Ultraviolet RaysEnzymology
Phylogeny17392226Comamonas odontotermitis sp. nov., isolated from the gut of the termite Odontotermes formosanus.Chou JH, Sheu SY, Lin KY, Chen WM, Arun AB, Young CCInt J Syst Evol Microbiol10.1099/ijs.0.64551-02007Animals, Comamonas/*classification/genetics/isolation & purification/physiology, DNA, Bacterial/genetics, DNA, Ribosomal/genetics, Digestive System/microbiology, Isoptera/*microbiology, Molecular Sequence Data, RNA, Ribosomal, 16S/geneticsGenetics
Phylogeny18175717Comamonas composti sp. nov., isolated from food waste compost.Young CC, Chou JH, Arun AB, Yen WS, Sheu SY, Shen FT, Lai WA, Rekha PD, Chen WMInt J Syst Evol Microbiol10.1099/ijs.0.65277-02008Bacterial Typing Techniques, Comamonas/*classification/genetics/isolation & purification/physiology, DNA, Bacterial/analysis, Fatty Acids/analysis, *Food Microbiology, Genes, rRNA, Genotype, Molecular Sequence Data, Nucleic Acid Hybridization, Phenotype, Phylogeny, RNA, Ribosomal, 16S/genetics, Refuse Disposal/*methods, Sequence Analysis, DNA, Species Specificity, TaiwanGenetics
Metabolism20554032Steroid degradation genes in Comamonas testosteroni TA441: Isolation of genes encoding a Delta4(5)-isomerase and 3alpha- and 3beta-dehydrogenases and evidence for a 100 kb steroid degradation gene hot spot.Horinouchi M, Kurita T, Hayashi T, Kudo TJ Steroid Biochem Mol Biol10.1016/j.jsbmb.2010.06.00220103-Hydroxysteroid Dehydrogenases/*genetics/metabolism, 3-alpha-Hydroxysteroid Dehydrogenase (B-Specific)/*genetics/metabolism, Chromatography, High Pressure Liquid, Comamonas testosteroni/*enzymology/*genetics, *Genes, Bacterial, Multigene Family, Mutation, Steroid Isomerases/*genetics/metabolism, Steroids/*metabolismEnzymology
Enzymology20678795Isolates of Comamonas spp. exhibiting catalase and peroxidase activities and diversity of their responses to oxidative stress.Buckova M, Godocikova J, Zamocky M, Polek BEcotoxicol Environ Saf10.1016/j.ecoenv.2010.07.0072010Catalase/*metabolism, Comamonas/drug effects/*enzymology/growth & development, Electrophoresis, Polyacrylamide Gel, Environmental Pollutants/*toxicity, Hydrogen Peroxide/toxicity, Oxidative Stress/*drug effects, Peroxidase/*metabolismMetabolism
Metabolism20970917Enhancement of cometabolic biodegradation of 4-chlorophenol induced with phenol and glucose as carbon sources by Comamonas testosteroni.Tobajas M, Monsalvo VM, Mohedano AF, Rodriguez JJJ Environ Manage10.1016/j.jenvman.2010.09.0302010*Biodegradation, Environmental, Chlorophenols/*metabolism, Comamonas testosteroni/*metabolism, Glucose/metabolism, Phenol/*metabolism
Enzymology21111045Cloning, expression and characterization of a novel short-chain dehydrogenase/reductase (SDRx) in Comamonas testosteroni.Gong W, Xiong G, Maser EJ Steroid Biochem Mol Biol10.1016/j.jsbmb.2010.11.0082010Amino Acid Sequence, Base Sequence, Cloning, Molecular, Comamonas testosteroni/*enzymology/genetics, Gene Expression Regulation, Bacterial, Gene Knockout Techniques, Hydroxysteroid Dehydrogenases/*genetics, Molecular Sequence Data, PhylogenyGenetics
Metabolism21185812Characterization of the steroid degrading bacterium S19-1 from the Baltic Sea at Kiel, Germany.Zhang T, Xiong G, Maser EChem Biol Interact10.1016/j.cbi.2010.12.0212010Anti-Bacterial Agents/pharmacology, Dose-Response Relationship, Drug, Drug Resistance, Bacterial, Enterobacteriaceae/drug effects/genetics/growth & development/*metabolism, Estradiol/metabolism, Germany, Plasmids/genetics, RNA, Bacterial/genetics, RNA, Ribosomal, 16S/genetics, Seawater/*microbiology, Sequence Analysis, RNA, Sodium Chloride/pharmacology, Steroids/*metabolism/pharmacology, Temperature, Testosterone/metabolism, Transformation, BacterialTranscriptome
Metabolism22156416Identification and characterization of the LysR-type transcriptional regulator HsdR for steroid-inducible expression of the 3alpha-hydroxysteroid dehydrogenase/carbonyl reductase gene in Comamonas testosteroni.Gong W, Xiong G, Maser EAppl Environ Microbiol10.1128/AEM.06872-1120113-Hydroxysteroid Dehydrogenases/*biosynthesis, Alcohol Oxidoreductases/*biosynthesis, Comamonas testosteroni/genetics/metabolism/*physiology, DNA, Bacterial/chemistry/genetics/metabolism, DNA-Binding Proteins/genetics/metabolism, Electrophoretic Mobility Shift Assay, Gene Deletion, *Gene Expression Regulation, Bacterial, Molecular Sequence Data, Protein Binding, Sequence Analysis, DNA, Steroids/*metabolism, Transcription Factors/genetics/*metabolismEnzymology
Genetics22374961Genome sequence of Comamonas testosteroni ATCC 11996, a representative strain involved in steroid degradation.Gong W, Kisiela M, Schilhabel MB, Xiong G, Maser EJ Bacteriol10.1128/JB.06795-112012Base Composition, Carbon/metabolism, Comamonas testosteroni/*genetics/metabolism, DNA, Bacterial/*chemistry/*genetics, *Genome, Bacterial, Molecular Sequence Data, Sequence Analysis, DNA, Steroids/metabolismMetabolism
Phylogeny22581903Comamonas guangdongensis sp. nov., isolated from subterranean forest sediment, and emended description of the genus Comamonas.Zhang J, Wang Y, Zhou S, Wu C, He J, Li FInt J Syst Evol Microbiol10.1099/ijs.0.040188-02012Bacterial Typing Techniques, Base Composition, China, Comamonas/*classification/genetics/isolation & purification, DNA, Bacterial/genetics, Fatty Acids/analysis, Molecular Sequence Data, Nucleic Acid Hybridization, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, *Soil Microbiology, Trees/microbiology, Ubiquinone/analysisGenetics
Metabolism23295223A novel transcriptional repressor PhaR for the steroid-inducible expression of the 3,17beta-hydroxysteroid dehydrogenase gene in Comamonas testosteroni ATCC11996.Li M, Xiong G, Maser EChem Biol Interact10.1016/j.cbi.2012.12.014201317-Hydroxysteroid Dehydrogenases/*genetics/*metabolism, Base Sequence, Cholesterol/metabolism, Cloning, Molecular, Comamonas testosteroni/*enzymology/*genetics/metabolism, Escherichia coli/enzymology/genetics/metabolism, Estradiol/metabolism, Molecular Sequence Data, Mutation/genetics, Plasmids/genetics, Promoter Regions, Genetic/genetics, Repressor Proteins/*genetics/metabolism, Sequence Analysis, DNA/methods, Silencer Elements, Transcriptional, Steroids/*metabolism, Testosterone/metabolism, Transcription, GeneticEnzymology
Enzymology23972763Cloning, expression and characterization of a putative 7alpha-hydroxysteroid dehydrogenase in Comamonas testosteroni.Ji W, Chen Y, Zhang H, Zhang X, Li Z, Yu YMicrobiol Res10.1016/j.micres.2013.07.0092013Amino Acid Sequence, Bacterial Proteins/*chemistry/*genetics/metabolism, Base Sequence, *Cloning, Molecular, Comamonas testosteroni/chemistry/*enzymology/genetics, Gene Expression Regulation, Bacterial, Hydroxysteroid Dehydrogenases/*chemistry/*genetics/metabolism, Molecular Sequence Data, Sequence AlignmentMetabolism
Phylogeny25242539Description of Comamonas serinivorans sp. nov., isolated from wheat straw compost.Zhu D, Xie C, Huang Y, Sun J, Zhang WInt J Syst Evol Microbiol10.1099/ijs.0.066688-02014Bacterial Typing Techniques, Base Composition, China, Comamonas/*classification/genetics/isolation & purification, DNA, Bacterial/genetics, Fatty Acids/chemistry, Molecular Sequence Data, Nucleic Acid Hybridization, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, *Soil Microbiology, Triticum/*microbiology, Ubiquinone/chemistryGenetics
Enzymology25446854Identification and isolation of a regulator protein for 3,17beta-HSD expressional regulation in Comamonas testosteroni.Wu Y, Huang P, Xiong G, Maser EChem Biol Interact10.1016/j.cbi.2014.11.004201417-Hydroxysteroid Dehydrogenases/*genetics, Amino Acid Sequence, Base Sequence, Cloning, Molecular/methods, Comamonas testosteroni/*enzymology/*genetics, DNA/genetics, Escherichia coli/enzymology/genetics, Fatty Acid Synthases/genetics, Molecular Sequence Data, NADH, NADPH Oxidoreductases/genetics, Promoter Regions, Genetic/genetics, Recombinant Proteins/genetics, Regulatory Sequences, Nucleic Acid/*genetics, Steroids/metabolism, Testosterone/geneticsGenetics
Enzymology25559855Isolation and identification of a repressor TetR for 3,17beta-HSD expressional regulation in Comamonas testosteroni.Pan T, Huang P, Xiong G, Maser EChem Biol Interact10.1016/j.cbi.2014.12.034201517-Hydroxysteroid Dehydrogenases/*genetics, Bacterial Proteins/*genetics, Base Sequence, Cloning, Molecular/methods, Comamonas testosteroni/*enzymology/*genetics, Escherichia coli/enzymology/genetics, Green Fluorescent Proteins/genetics, Mutation/genetics, Repressor Proteins, Steroids/metabolism, Testosterone/geneticsPhylogeny
Enzymology25595225Cloning and characterization of a novel beta-ketoacyl-ACP reductase from Comamonas testosteroni.Zhang H, Ji Y, Wang Y, Zhang X, Yu YChem Biol Interact10.1016/j.cbi.2015.01.00320153-Oxoacyl-(Acyl-Carrier-Protein) Reductase/*genetics, Alcohol Dehydrogenase/genetics, Amino Acid Sequence, Base Sequence, Cholesterol/genetics, Cloning, Molecular/methods, Comamonas testosteroni/*enzymology/*genetics, Escherichia coli/enzymology/genetics, Estradiol/genetics, Estriol/genetics, Fatty Acid Synthases/genetics, NADH, NADPH Oxidoreductases/genetics, Plasmids/genetics, Sequence Alignment, Steroids/metabolism, Testosterone/geneticsMetabolism
Enzymology25595227Characterization of 3,17beta-hydroxysteroid dehydrogenase in Comamonas testosteroni.Yu Y, Liu C, Wang B, Li Y, Zhang HChem Biol Interact10.1016/j.cbi.2015.01.005201517-Hydroxysteroid Dehydrogenases/*genetics, Amino Acid Sequence, Base Sequence, Cholesterol/genetics, Cloning, Molecular/methods, Comamonas testosteroni/*enzymology/*genetics, Escherichia coli/enzymology/genetics, Estradiol/genetics, Gene Knockout Techniques/methods, Molecular Sequence Data, Mutation/genetics, Promoter Regions, Genetic/genetics, Sequence Alignment, Steroids/metabolism, Testosterone/geneticsGenetics
Enzymology25614138Cloning, expression and characterization of a putative 2,5-diketo-D-gluconic acid reductase in Comamonas testosteroni.Chen Y, Ji W, Zhang H, Zhang X, Yu YChem Biol Interact10.1016/j.cbi.2015.01.0172015Aldehyde Reductase/*genetics, Aldo-Keto Reductases, Amino Acid Sequence, Base Sequence, Binding Sites, Cloning, Molecular/methods, Comamonas testosteroni/*enzymology/*genetics, Escherichia coli/enzymology/genetics, Molecular Sequence Data, Mutation/genetics, NAD/genetics, NADP/genetics, Phylogeny, Plasmids/genetics, Sequence Alignment, Steroids/metabolism, Sugar Alcohol Dehydrogenases/*geneticsGenetics
Enzymology28274720Functional analysis of a novel repressor LuxR in Comamonas testosteroni.Ji Y, Pan T, Zhang Y, Xiong G, Yu YChem Biol Interact10.1016/j.cbi.2017.03.002201717-Hydroxysteroid Dehydrogenases/genetics/metabolism, Amino Acid Sequence, Cloning, Molecular, Comamonas testosteroni/drug effects/*enzymology/growth & development, Escherichia coli/metabolism, Gene Knockout Techniques, Plasmids/genetics/metabolism, Recombinant Proteins/biosynthesis/chemistry/isolation & purification, Repressor Proteins/chemistry/genetics/*metabolism, Temperature, Testosterone/pharmacology, Trans-Activators/chemistry/genetics/*metabolism, Up-Regulation/drug effectsMetabolism
Metabolism28532684Quantitative determination of testosterone levels with biolayer interferometry.Zhang H, Li W, Luo H, Xiong G, Yu YChem Biol Interact10.1016/j.cbi.2017.05.0132017Bacterial Proteins/chemistry/genetics/metabolism, Biotin/chemistry/metabolism, Comamonas testosteroni/genetics, DNA Helicases/chemistry/genetics/metabolism, DNA, Bacterial/chemistry, Immobilized Nucleic Acids/chemistry/metabolism, *Interferometry, Kinetics, Recombinant Proteins/biosynthesis/chemistry/isolation & purification, Streptavidin/metabolism, Testosterone/*analysis/metabolism, Trans-Activators/chemistry/genetics/metabolismEnzymology
Enzymology32215256The characterization of a short chain dehydrogenase/reductase (SDRx) in Comamonas testosteroni.Liu C, Liu K, Zhao C, Gong P, Yu YToxicol Rep10.1016/j.toxrep.2020.02.0152020
Metabolism33002461Characterization of a LuxR repressor for 3,17beta-HSD in Comamonas testosteroni ATCC11996.Ji Y, Yang J, Gao L, Xiong G, Yu Y, Zhang YChem Biol Interact10.1016/j.cbi.2020.109271202017-Hydroxysteroid Dehydrogenases/*metabolism, Comamonas testosteroni/cytology/*enzymology/growth & development, Models, Molecular, Point Mutation, Protein Conformation, Repressor Proteins/chemistry/deficiency/*metabolism, Trans-Activators/chemistry/deficiency/*metabolismEnzymology
Enzymology34677711Cloning and identification of a new repressor of 3,17beta-Hydroxysteroid dehydrogenase of Comamonas testosteroni.Xie W, Xia Q, Chen L, Xiong G, Gao Y, Yu Y, He XMol Biol Rep10.1007/s11033-021-06566-9202117-Hydroxysteroid Dehydrogenases/*antagonists & inhibitors, *Bacterial Proteins/biosynthesis/chemistry/genetics, *Cloning, Molecular, *Comamonas testosteroni/chemistry/genetics/metabolism, *Enzyme Inhibitors/chemistry/metabolism, Recombinant Proteins/biosynthesis/chemistry/genetics, *Trans-Activators/biosynthesis/chemistry/geneticsMetabolism
Phylogeny35316175Comamonas fluminis sp. nov., isolated from the Han River, Republic of Korea.Park EH, Kim YS, Cha CJInt J Syst Evol Microbiol10.1099/ijsem.0.0052872022Bacterial Typing Techniques, Base Composition, *Comamonas/genetics, DNA, Bacterial/genetics, Fatty Acids/chemistry, Phospholipids/chemistry, Phylogeny, RNA, Ribosomal, 16S/genetics, *Rivers/microbiology, Sequence Analysis, DNATranscriptome

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
12682Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 50244)https://www.dsmz.de/collection/catalogue/details/culture/DSM-50244
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
37573Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/9765
44373Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 1426)https://www.ccug.se/strain?id=1426
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68369Automatically annotated from API 20NE
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch10.21203/rs.3.rs-2527258/v1Predictions based on the model GenomeNet Sporulation v. 1
72446Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID2054.1StrainInfo: A central database for resolving microbial strain identifiers
123485Curators of the CIPCollection of Institut Pasteur (CIP 59.24)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%2059.24