Strain identifier

BacDive ID: 2849

Type strain: Yes

Species: Enterocloster lavalensis

Strain Designation: CCRI-9842

Strain history: CIP <- 2007, CCUG <- CHUL Bergeron, Quebec, Canada: strain CCRI-9842

NCBI tax ID(s): 460384 (species)

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General

@ref: 8290

BacDive-ID: 2849

DSM-Number: 19851

keywords: genome sequence, 16S sequence, Bacteria, anaerobe, spore-forming, mesophilic, Gram-positive, motile, rod-shaped

description: Enterocloster lavalensis CCRI-9842 is an anaerobe, spore-forming, mesophilic bacterium that was isolated from human faeces, rectal swab.

NCBI tax id

  • NCBI tax id: 460384
  • Matching level: species

strain history

@refhistory
8290<- M.-C. Domingo, Centre de recherche en infectiologie, Québec, Canada; CCRI-9842 <- A. Bernal {2004}
67770CCUG 54291 <-- M.-C. Domingo and M. G. Bergeron CCRI-9842 <-- A. Bernal.
120574CIP <- 2007, CCUG <- CHUL Bergeron, Quebec, Canada: strain CCRI-9842

doi: 10.13145/bacdive2849.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Clostridia
  • order: Eubacteriales
  • family: Lachnospiraceae
  • genus: Enterocloster
  • species: Enterocloster lavalensis
  • full scientific name: Enterocloster lavalensis (Domingo et al. 2009) Haas and Blanchard 2020

  • synonyms

    • @ref: 20215
    • synonym: Clostridium lavalense

@ref: 8290

domain: Bacteria

phylum: Firmicutes

class: Clostridia

order: Clostridiales

family: Lachnospiraceae

genus: Enterocloster

species: Enterocloster lavalensis

full scientific name: Enterocloster lavalensis (Domingo et al. 2009) Haas and Blanchard 2020

strain designation: CCRI-9842

type strain: yes

Morphology

cell morphology

@refgram staincell lengthcell widthcell shapemotility
28932positive2.5-6.0 µm0.8-1.5 µmrod-shapedyes
120574positiverod-shapedno

colony morphology

  • @ref: 60125
  • incubation period: 2 days

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
8290BHI MEDIUM FOR STRICT ANAEROBES (DSMZ Medium 215c)yeshttps://mediadive.dsmz.de/medium/215cName: BHI MEDIUM FOR STRICT ANAEROBES (DSMZ Medium 215c; with strain-specific modifications) Composition: Brain heart infusion 37.0 g/l Na2S x 9 H2O 0.25 g/l L-Cysteine HCl x H2O 0.25 g/l Haemin Distilled water
37748MEDIUM 20 - for Anaerobic bacteriayesAgar (15.000 g);Glucose (5.000 g);Yeast extract (20.000 g);Tryptone (30.000 g);Cysteine hydrochloride (0.500 g);distilled water (1000.000 ml);Hemin solution -M00149 (25.000 ml)
120574CIP Medium 20yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=20

culture temp

@refgrowthtypetemperaturerange
8290positivegrowth35mesophilic
28932positivegrowth35-37mesophilic
28932positiveoptimum36mesophilic
37748positivegrowth37mesophilic
60125positivegrowth37mesophilic
67770positivegrowth37mesophilic

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
8290anaerobe
28932anaerobe
60125anaerobe
120574anaerobe

spore formation

  • @ref: 28932
  • spore formation: yes

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
2893222599arabinose+carbon source
2893228757fructose+carbon source
2893228260galactose+carbon source
2893217234glucose+carbon source
2893217716lactose+carbon source
2893217306maltose+carbon source
2893237684mannose+carbon source
2893233942ribose+carbon source
2893217814salicin+carbon source
2893230911sorbitol+carbon source
2893217992sucrose+carbon source
2893227082trehalose+carbon source
2893218222xylose+carbon source
2893217632nitrate+reduction
12057417632nitrate-reduction
12057416301nitrite-reduction

metabolite production

@refChebi-IDmetaboliteproduction
2893235581indoleyes
12057435581indoleno

metabolite tests

  • @ref: 28932
  • Chebi-ID: 35581
  • metabolite: indole
  • indole test: +

enzymes

@refvalueactivityec
68382alpha-fucosidase-3.2.1.51
68382alpha-mannosidase-3.2.1.24
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382beta-glucosidase-3.2.1.21
68382alpha-glucosidase-3.2.1.20
68382beta-glucuronidase-3.2.1.31
68382beta-galactosidase-3.2.1.23
68382alpha-galactosidase-3.2.1.22
68382naphthol-AS-BI-phosphohydrolase+
68382acid phosphatase+3.1.3.2
68382alpha-chymotrypsin-3.4.21.1
68382trypsin-3.4.21.4
68382cystine arylamidase-3.4.11.3
68382valine arylamidase-
68382leucine arylamidase-3.4.11.1
68382lipase (C 14)-
68382esterase lipase (C 8)-
68382esterase (C 4)-
68382alkaline phosphatase-3.1.3.1
120574oxidase-
120574catalase-1.11.1.6
120574urease-3.5.1.5

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
120574----------++--------

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinentsampling date
8290human faeces, rectal swabQuébec, Québec cityCanadaCANNorth America
60125Human fecesQuébecCanadaCANNorth America2001-10-01
67770Human fecesQuébecCanadaCANNorth America
120574Human, FecesQuebecCanadaCANNorth America

isolation source categories

Cat1Cat2Cat3
#Infection#Patient#Swab
#Host#Human
#Host Body-Site#Gastrointestinal tract#Rectum
#Host Body Product#Gastrointestinal tract#Feces (Stool)

taxonmaps

  • @ref: 69479
  • File name: preview.99_2066.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_16;96_176;97_1334;98_1616;99_2066&stattab=map
  • Last taxonomy: Enterocloster
  • 16S sequence: EF564277
  • Sequence Identity:
  • Total samples: 43647
  • soil counts: 86
  • aquatic counts: 422
  • animal counts: 43046
  • plant counts: 93

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
82901Risk group (German classification)
1205742Risk group (French classification)

Sequence information

16S sequences

  • @ref: 8290
  • description: Clostridium lavalense strain CCRI-9842 16S ribosomal RNA gene, partial sequence
  • accession: EF564277
  • length: 1454
  • database: ena
  • NCBI tax ID: 460384

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792[Clostridium] lavalense strain KCTC 15153460384.7wgspatric460384
67770Enterocloster lavalensis KCTC 15153GCA_003024655contigncbi460384

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
gram-positiveyes89.696yes
anaerobicyes98.779no
halophileno87.158no
spore-formingyes62.345no
glucose-utilyes91.683no
aerobicno97.532no
flagellatedno91.682no
thermophileno98.939yes
motileno82.359no
glucose-fermentyes72.063no

External links

@ref: 8290

culture collection no.: DSM 19851, CCUG 54291, JCM 14986, NML 03-A-015, CIP 109511, KCTC 15153

straininfo link

  • @ref: 72373
  • straininfo: 361149

literature

  • topic: Phylogeny
  • Pubmed-ID: 19244429
  • title: Clostridium lavalense sp. nov., a glycopeptide-resistant species isolated from human faeces.
  • authors: Domingo MC, Huletsky A, Boissinot M, Helie MC, Bernal A, Bernard KA, Grayson ML, Picard FJ, Bergeron MG
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/ijs.0.001958-0
  • year: 2009
  • mesh: Anti-Bacterial Agents/*pharmacology, Bacterial Typing Techniques, Clostridium/*classification/*drug effects/genetics/isolation & purification, DNA, Bacterial/analysis, *Drug Resistance, Bacterial, Fatty Acids/analysis, Feces/*microbiology, Glycopeptides/*pharmacology, Humans, Microbial Sensitivity Tests, Molecular Sequence Data, Phenotype, Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Species Specificity
  • topic2: Pathogenicity

Reference

@idauthorscataloguedoi/urltitleID_cross_referencepubmed
8290Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 19851)https://www.dsmz.de/collection/catalogue/details/culture/DSM-19851
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
28932Barberan A, Caceres Velazquez H, Jones S, Fierer N.10.1128/mSphere.00237-17Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information2537128776041
37748Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/7263
60125Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 54291)https://www.ccug.se/strain?id=54291
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
72373Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID361149.1StrainInfo: A central database for resolving microbial strain identifiers
120574Curators of the CIPCollection of Institut Pasteur (CIP 109511)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20109511