Strain identifier

BacDive ID: 2806

Type strain: Yes

Species: Anaerotignum lactatifermentans

Strain Designation: G17

Strain history: <- P. W. J. J. van der Wielen; G17 <- G. Rovers and P. W. J. J. van der Wielen {1998}

NCBI tax ID(s): 1121323 (strain), 160404 (species)

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General

@ref: 5253

BacDive-ID: 2806

DSM-Number: 14214

keywords: genome sequence, 16S sequence, Bacteria, anaerobe, chemoorganotroph, mesophilic, Gram-negative, rod-shaped

description: Anaerotignum lactatifermentans G17 is an anaerobe, chemoorganotroph, mesophilic bacterium that was isolated from caeca of broiler chicken.

NCBI tax id

NCBI tax idMatching level
160404species
1121323strain

strain history

  • @ref: 5253
  • history: <- P. W. J. J. van der Wielen; G17 <- G. Rovers and P. W. J. J. van der Wielen {1998}

doi: 10.13145/bacdive2806.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Clostridia
  • order: Eubacteriales
  • family: Lachnospiraceae
  • genus: Anaerotignum
  • species: Anaerotignum lactatifermentans
  • full scientific name: Anaerotignum lactatifermentans (van der Wielen et al. 2002) Ueki et al. 2017

  • synonyms

    • @ref: 20215
    • synonym: Clostridium lactatifermentans

@ref: 5253

domain: Bacteria

phylum: Firmicutes

class: Clostridia

order: Clostridiales

family: Lachnospiraceae

genus: Anaerotignum

species: Anaerotignum lactatifermentans

full scientific name: Anaerotignum lactatifermentans (van der Wielen et al. 2002) Ueki et al. 2017

strain designation: G17

type strain: yes

Morphology

cell morphology

@refgram staincell lengthcell widthcell shapemotilityconfidence
43524negative2.8-7.5 µm0.6-1.3 µmrod-shapedno
69480positive100

Culture and growth conditions

culture medium

  • @ref: 5253
  • name: PY + X MEDIUM (DSMZ Medium 104b)
  • growth: yes
  • link: https://mediadive.dsmz.de/medium/104b
  • composition: Name: PY + X MEDIUM (DSMZ Medium 104b) Composition: Yeast extract 10.0 g/l D-Glucose 5.0 g/l Trypticase peptone 5.0 g/l Meat peptone 5.0 g/l L-Cysteine HCl x H2O 0.5 g/l NaHCO3 0.4 g/l NaCl 0.08 g/l KH2PO4 0.04 g/l K2HPO4 0.04 g/l MgSO4 x 7 H2O 0.02 g/l CaCl2 x 2 H2O 0.01 g/l Sodium resazurin 0.0005 g/l Distilled water

culture temp

  • @ref: 5253
  • growth: positive
  • type: growth
  • temperature: 37
  • range: mesophilic

Physiology and metabolism

oxygen tolerance

@refoxygen toleranceconfidence
5253anaerobe
43524obligate anaerobe
69480anaerobe99.999

nutrition type

  • @ref: 43524
  • type: chemoorganotroph

spore formation

@refspore formationconfidence
69481no100
69480no97.803

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
4352416236ethanol-assimilation
4352416977L-alanine+assimilation
4352417115L-serine+assimilation
4352416857L-threonine+assimilation
4352418222xylose+assimilation
4352417234glucose+/-assimilation
4352417191L-isoleucine+/-assimilation
4352415603L-leucine+/-assimilation
4352416414L-valine+/-assimilation

metabolite production

@refChebi-IDmetaboliteproduction
4352430089acetateyes
4352417968butyrateyes
4352448944isobutyrateyes
4352448942isovalerateyes
4352417272propionateyes

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinent
5253caeca of broiler chickenLeusdenNetherlandsNLDEurope
43524chicken caeca

isolation source categories

Cat1Cat2Cat3
#Host Body-Site#Gastrointestinal tract#Large intestine
#Host#Birds#Chicken
#Host Body Product#Gastrointestinal tract#Caecal content

Safety information

risk assessment

  • @ref: 5253
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

  • @ref: 5253
  • description: Clostridium lactatifermentans 16S ribosomal RNA gene, partial sequence
  • accession: AY033434
  • length: 1497
  • database: ena
  • NCBI tax ID: 160404

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Anaerotignum lactatifermentans DSM 14214GCA_900142265scaffoldncbi1121323
66792[Clostridium] lactatifermentans DSM 142141121323.3wgspatric1121323
66792Anaerotignum lactatifermentans DSM 142142585428190draftimg1121323

GC content

@refGC-contentmethod
4352446high performance liquid chromatography (HPLC)
525344.6high performance liquid chromatography (HPLC)

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingno100no
motileno90.747yes
flagellatedno94.481yes
gram-positiveyes82.625yes
anaerobicyes99.59no
aerobicno98.764yes
halophileno74.511no
spore-formingno66.11no
thermophileno98.094yes
glucose-utilyes79.32no
glucose-fermentyes54.649no

External links

@ref: 5253

culture collection no.: DSM 14214, LMG 20954

straininfo link

  • @ref: 72332
  • straininfo: 84993

literature

  • topic: Phylogeny
  • Pubmed-ID: 28905695
  • title: Description of Anaerotignum aminivorans gen. nov., sp. nov., a strictly anaerobic, amino-acid-decomposing bacterium isolated from a methanogenic reactor, and reclassification of Clostridium propionicum, Clostridium neopropionicum and Clostridium lactatifermentans as species of the genus Anaerotignum.
  • authors: Ueki A, Goto K, Ohtaki Y, Kaku N, Ueki K
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/ijsem.0.002268
  • year: 2017
  • mesh: Animals, Bacterial Typing Techniques, Base Composition, Bioreactors/microbiology, Cattle, Clostridiales/*classification/genetics/isolation & purification, Clostridium/*classification, DNA, Bacterial/genetics, Diaminopimelic Acid/chemistry, Fatty Acids/chemistry, Japan, Peptidoglycan/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Sewage/*microbiology, Waste Disposal, Fluid
  • topic2: Transcriptome

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
5253Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 14214)https://www.dsmz.de/collection/catalogue/details/culture/DSM-14214
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
43524Atsuko Ueki, Kazushi Goto, Yoshimi Ohtaki, Nobuo Kaku, Katsuji Ueki10.1099/ijsem.0.002268Description of Anaerotignum aminivorans gen. nov., sp. nov., a strictly anaerobic, amino-acid-decomposing bacterium isolated from a methanogenic reactor, and reclassification of Clostridium propionicum, Clostridium neopropionicum and Clostridium lactatifermentans as species of the genus AnaerotignumIJSEM 67: 4146-4153 201728905695
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch10.21203/rs.3.rs-2527258/v1Predictions based on the model GenomeNet Sporulation v. 1
72332Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID84993.1StrainInfo: A central database for resolving microbial strain identifiers