Strain identifier

BacDive ID: 2745

Type strain: Yes

Species: Clostridium haemolyticum

Strain Designation: Mc Clung 2027, 2027, McClung, 29866 (Folsum), 2027, McClung

Strain history: ATCC 9650 <-- H. Plummer 29866 (Folsom) <-- L. R. Vawter (L. S. McClung 2027).

NCBI tax ID(s): 84025 (species)

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General

@ref: 2221

BacDive-ID: 2745

DSM-Number: 5565

keywords: 16S sequence, Bacteria, anaerobe, mesophilic, human pathogen

description: Clostridium haemolyticum Mc Clung 2027 is an anaerobe, mesophilic human pathogen that was isolated from bovine.

NCBI tax id

  • NCBI tax id: 84025
  • Matching level: species

strain history

@refhistory
2221<- ATCC <- H. Plummer, 29866 (Folsum) <- L. Vawter
67770ATCC 9650 <-- H. Plummer 29866 (Folsom) <-- L. R. Vawter (L. S. McClung 2027).

doi: 10.13145/bacdive2745.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Clostridia
  • order: Eubacteriales
  • family: Clostridiaceae
  • genus: Clostridium
  • species: Clostridium haemolyticum
  • full scientific name: Clostridium haemolyticum corrig. (Hall 1929) Scott et al. 1935 (Approved Lists 1980)

  • synonyms

    @refsynonym
    20215Bacillus hemolyticus
    20215Clostridium hemolyticum

@ref: 2221

domain: Bacteria

phylum: Firmicutes

class: Clostridia

order: Clostridiales

family: Clostridiaceae

genus: Clostridium

species: Clostridium haemolyticum

full scientific name: Clostridium haemolyticum (Hall 1929) Scott et al. 1935

strain designation: Mc Clung 2027, 2027, McClung, 29866 (Folsum), 2027, McClung

type strain: yes

Culture and growth conditions

culture medium

  • @ref: 2221
  • name: CHOPPED MEAT MEDIUM WITH CARBOHYDRATES (DSMZ Medium 110)
  • growth: yes
  • link: https://mediadive.dsmz.de/medium/110
  • composition: Name: CHOPPED MEAT MEDIUM WITH CARBOHYDRATES (DSMZ Medium 110) Composition: Ground beef 500.0 g/l Casitone 30.0 g/l Agar 15.0 g/l K2HPO4 5.0 g/l Yeast extract 5.0 g/l D-Glucose 4.0 g/l Starch 1.0 g/l Maltose 1.0 g/l Cellobiose 1.0 g/l L-Cysteine HCl 0.5 g/l Ethanol 0.19 g/l Vitamin K3 0.05 g/l Hemin 0.005 g/l Sodium resazurin 0.0005 g/l Vitamin K1 NaOH Distilled water

culture temp

@refgrowthtypetemperaturerange
2221positivegrowth37mesophilic
67770positivegrowth37mesophilic

Physiology and metabolism

oxygen tolerance

  • @ref: 2221
  • oxygen tolerance: anaerobe

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
6836727082trehalose-builds acid from
6836762345L-rhamnose-builds acid from
6836730911sorbitol-builds acid from
6836716634raffinose-builds acid from
683676731melezitose-builds acid from
6836716024D-mannose-builds acid from
6836717057cellobiose-builds acid from
6836717754glycerol-builds acid from
683674853esculin-hydrolysis
683675291gelatin+hydrolysis
6836730849L-arabinose-builds acid from
6836765327D-xylose-builds acid from
6836717814salicin-builds acid from
6836717306maltose-builds acid from
6836717992sucrose-builds acid from
6836717716lactose-builds acid from
6836716899D-mannitol-builds acid from
6836717634D-glucose-builds acid from
6836716199urea-hydrolysis
6836727897tryptophan+energy source

metabolite production

  • @ref: 68367
  • Chebi-ID: 35581
  • metabolite: indole
  • production: yes

metabolite tests

  • @ref: 68367
  • Chebi-ID: 35581
  • metabolite: indole
  • indole test: +

enzymes

@refvalueactivityec
68367beta-glucosidase-3.2.1.21
68367gelatinase+
68367urease-3.5.1.5

API 20A

@refINDUREGLUMANLACSACMALSALXYLARAGELESCGLYCELMNEMLZRAFSORRHATRE
2221+---------+---------

Isolation, sampling and environmental information

isolation

@refsample type
2221bovine
67770Bovine clinical specimen

isolation source categories

  • Cat1: #Host
  • Cat2: #Mammals
  • Cat3: #Bovinae (Cow, Cattle)

taxonmaps

  • @ref: 69479
  • File name: preview.99_907.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_34;96_477;97_545;98_716;99_907&stattab=map
  • Last taxonomy: Clostridium
  • 16S sequence: Y18173
  • Sequence Identity:
  • Total samples: 939
  • soil counts: 53
  • aquatic counts: 142
  • animal counts: 741
  • plant counts: 3

Safety information

risk assessment

  • @ref: 2221
  • pathogenicity human: yes
  • pathogenicity animal: yes
  • biosafety level: 2
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Clostridium haemolyticum genes for 16S rRNA, 16S-23S rRNA spacer region (rrn1), 23S rRNA, partial sequenceAB040728255ena84025
20218Clostridium haemolyticum genes for 16S rRNA, tRNA-Ala, tRNA-Ile, 23S rRNA, partial and complete sequenceAB040729413ena84025
20218Clostridium haemolyticum genes for 16S rRNA, tRNA-Ala, tRNA-Ile, 23S rRNA, partial and complete sequenceAB040730467ena84025
20218Clostridium haemolyticum strain DSM 5565 16S ribosomal RNA gene, partial sequenceHM0075881339ena84025
20218Clostridium haemolyticum strain DSM 5565 16S ribosomal RNA gene, partial sequenceHM245943841ena84025
20218Clostridium haemolyticum 16S rRNA gene, partial, strain DSM 5565Y181731469ena84025
2221Clostridium haemolyticum strain DSM 5565 16S ribosomal RNA, partial sequenceNR_1177671339nuccore84025
67770Clostridium haemolyticum gene for 16S rRNA, partial sequenceAB0379101465ena84025
67770Clostridium haemolyticum gene for 16S ribosomal RNA, partial sequence, strain: JCM 1402AB6406871474ena84025

GC content

  • @ref: 2221
  • GC-content: 26

External links

@ref: 2221

culture collection no.: DSM 5565, ATCC 9650, NCIB 10664, JCM 1402, BCRC 10643, NCIMB 10664, NCTC 13022

straininfo link

  • @ref: 72275
  • straininfo: 35089

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
2221Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 5565)https://www.dsmz.de/collection/catalogue/details/culture/DSM-5565
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68367Automatically annotated from API 20A
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
72275Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID35089.1StrainInfo: A central database for resolving microbial strain identifiers