Strain identifier
BacDive ID: 2733
Type strain: 
Species: Thermoclostridium stercorarium subsp. thermolacticum
Strain Designation: TX41
Strain history: <- P. Le Ruyet and H. C. Dubourguier, Station de Technologie Alimentaire, Villeneuve D'Ascq, France; TX41 <- P. Le Ruyet {1982}
NCBI tax ID(s): 1121336 (strain), 29351 (subspecies)
version 8.1 (current version)
General
@ref: 1243
BacDive-ID: 2733
DSM-Number: 2910
keywords: genome sequence, 16S sequence, Bacteria, anaerobe, thermophilic, Gram-positive
description: Thermoclostridium stercorarium subsp. thermolacticum TX41 is an anaerobe, thermophilic, Gram-positive bacterium that was isolated from mesophilic digester fed with Lemna minor.
NCBI tax id
| NCBI tax id | Matching level |
|---|---|
| 1121336 | strain |
| 29351 | subspecies |
strain history
- @ref: 1243
- history: <- P. Le Ruyet and H. C. Dubourguier, Station de Technologie Alimentaire, Villeneuve D'Ascq, France; TX41 <- P. Le Ruyet {1982}
doi: 10.13145/bacdive2733.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/bacillota
- domain: Bacteria
- phylum: Bacillota
- class: Clostridia
- order: Eubacteriales
- family: Oscillospiraceae
- genus: Thermoclostridium
- species: Thermoclostridium stercorarium subsp. thermolacticum
- full scientific name: Thermoclostridium stercorarium subsp. thermolacticum (Le Ruyet et al. 1988) Zhang et al. 2018
synonyms
@ref synonym 20215 Clostridium stercorarium subsp. thermolacticum 20215 Clostridium thermolacticum
@ref: 1243
domain: Bacteria
phylum: Firmicutes
class: Clostridia
order: Clostridiales
family: Oscillospiraceae
genus: Thermoclostridium
species: Thermoclostridium stercorarium subsp. thermolacticum
full scientific name: Thermoclostridium stercorarium subsp. thermolacticum (Le Ruyet et al. 1988) Zhang et al. 2018
strain designation: TX41
type strain: yes
Morphology
cell morphology
- @ref: 69480
- gram stain: positive
- confidence: 99.72
Culture and growth conditions
culture medium
- @ref: 1243
- name: THERMOCLOSTRIDIUM (A-GÖ) MEDIUM (DSMZ Medium 326)
- growth: yes
- link: https://mediadive.dsmz.de/medium/326
- composition: Name: THERMOCLOSTRIDIUM (A-GÖ) MEDIUM (DSMZ Medium 326) Composition: Sucrose 4.99002 g/l KH2PO4 3.29341 g/l Yeast extract 1.99601 g/l Trypticase peptone 1.99601 g/l Na2CO3 0.998004 g/l Na2S x 9 H2O 0.299401 g/l L-Cysteine HCl x H2O 0.299401 g/l MgCl2 x 6 H2O 0.159681 g/l HCl 0.00249501 g/l FeCl2 x 4 H2O 0.00149701 g/l Sodium resazurin 0.000499002 g/l CoCl2 x 6 H2O 0.000189621 g/l Pyridoxine hydrochloride 9.98004e-05 g/l MnCl2 x 4 H2O 9.98004e-05 g/l ZnCl2 6.98603e-05 g/l Nicotinic acid 4.99002e-05 g/l (DL)-alpha-Lipoic acid 4.99002e-05 g/l Calcium D-(+)-pantothenate 4.99002e-05 g/l Thiamine HCl 4.99002e-05 g/l Riboflavin 4.99002e-05 g/l p-Aminobenzoic acid 4.99002e-05 g/l Na2MoO4 x 2 H2O 3.59281e-05 g/l NiCl2 x 6 H2O 2.39521e-05 g/l Biotin 1.99601e-05 g/l Folic acid 1.99601e-05 g/l H3BO3 5.98802e-06 g/l CuCl2 x 2 H2O 1.99601e-06 g/l Vitamin B12 9.98004e-07 g/l Distilled water
culture temp
| @ref | growth | type | temperature | range | confidence |
|---|---|---|---|---|---|
| 1243 | positive | growth | 60 | thermophilic | |
| 69480 | thermophilic | 99.627 |
Physiology and metabolism
oxygen tolerance
| @ref | oxygen tolerance | confidence |
|---|---|---|
| 1243 | anaerobe | |
| 69480 | anaerobe | 99.998 |
Isolation, sampling and environmental information
isolation
- @ref: 1243
- sample type: mesophilic digester fed with Lemna minor
- host species: Lemna minor
- country: France
- origin.country: FRA
- continent: Europe
isolation source categories
| Cat1 | Cat2 | Cat3 |
|---|---|---|
| #Engineered | #Biodegradation | |
| #Host | #Plants | #Aquatic plant |
taxonmaps
- @ref: 69479
- File name: preview.99_1650.png
- url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_246;96_941;97_1095;98_1306;99_1650&stattab=map
- Last taxonomy: Thermoclostridium stercorarium subclade
- 16S sequence: X72870
- Sequence Identity:
- Total samples: 6000
- soil counts: 1258
- aquatic counts: 584
- animal counts: 3737
- plant counts: 421
Safety information
risk assessment
- @ref: 1243
- biosafety level: 1
- biosafety level comment: Risk group (German classification)
Sequence information
16S sequences
- @ref: 1243
- description: C.thermolacticum gene for 16S rRNA
- accession: X72870
- length: 1517
- database: ena
- NCBI tax ID: 1121336
Genome sequences
| @ref | description | accession | assembly level | database | NCBI tax ID |
|---|---|---|---|---|---|
| 66792 | Thermoclostridium stercorarium subsp. thermolacticum DSM 2910 | GCA_001697245 | complete | ncbi | 1121336 |
| 66792 | [Clostridium] stercorarium subsp. thermolacticum DSM 2910 | 1121336.3 | complete | patric | 1121336 |
GC content
- @ref: 1243
- GC-content: 40.9
Genome-based predictions
predictions
| trait | prediction | confidence | training_data |
|---|---|---|---|
| spore-forming | no | 72 | no |
| flagellated | no | 78.802 | no |
| gram-positive | yes | 68.262 | no |
| anaerobic | yes | 99.003 | yes |
| halophile | no | 93.815 | no |
| spore-forming | yes | 86.805 | no |
| glucose-util | yes | 88.682 | no |
| aerobic | no | 98.39 | no |
| thermophile | yes | 99.681 | no |
| motile | yes | 72.366 | no |
| glucose-ferment | no | 51.236 | no |
External links
@ref: 1243
culture collection no.: DSM 2910, ATCC 43739
straininfo link
- @ref: 72262
- straininfo: 42014
literature
| topic | Pubmed-ID | title | authors | journal | DOI | year | mesh | topic2 |
|---|---|---|---|---|---|---|---|---|
| Metabolism | 10784299 | Anaerobic thermophilic fermentation for acetic acid production from milk permeate. | Talabardon M, Schwitzguebel JP, Peringer P | J Biotechnol | 10.1016/s0168-1656(99)00180-7 | 2000 | Acetic Acid/*metabolism, Anaerobiosis, Animals, Bioreactors, Biotechnology, Clostridium/metabolism, Fermentation, Lactose/metabolism, Milk/*metabolism | Biotechnology |
| Metabolism | 11760953 | Metabolism of cinnamic acids by some Clostridiales and emendation of the descriptions of Clostridium aerotolerans, Clostridium celerecrescens and Clostridium xylanolyticum. | Chamkha M, Garcia JL, Labat M | Int J Syst Evol Microbiol | 10.1099/00207713-51-6-2105 | 2001 | Bacterial Typing Techniques, Cinnamates/*metabolism, Clostridium/*classification/growth & development/*metabolism, Culture Media, Glucose/metabolism | Cultivation |
Reference
| @id | authors | catalogue | doi/url | title |
|---|---|---|---|---|
| 1243 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 2910) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-2910 | |
| 20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | |
| 66792 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | |
| 69479 | João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich. | https://microbeatlas.org/ | MicrobeAtlas 1.0 beta | |
| 69480 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | |
| 72262 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID42014.1 | StrainInfo: A central database for resolving microbial strain identifiers |