Strain identifier

BacDive ID: 2724

Type strain: Yes

Species: Clostridium tertium

Strain Designation: 795

Strain history: T. Mitsuoka <-- ATCC 14573 <-- J. D. MacLennan <-- L. McClung <-- R. Spray.

NCBI tax ID(s): 1559 (species)

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General

@ref: 1094

BacDive-ID: 2724

DSM-Number: 2485

keywords: genome sequence, 16S sequence, Bacteria, anaerobe, spore-forming, human pathogen

description: Clostridium tertium 795 is an anaerobe, spore-forming human pathogen of the family Clostridiaceae.

NCBI tax id

  • NCBI tax id: 1559
  • Matching level: species

strain history

@refhistory
1094<- NCIB <- G. Hobbs; 795
67770T. Mitsuoka <-- ATCC 14573 <-- J. D. MacLennan <-- L. McClung <-- R. Spray.

doi: 10.13145/bacdive2724.20240916.9.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Clostridia
  • order: Eubacteriales
  • family: Clostridiaceae
  • genus: Clostridium
  • species: Clostridium tertium
  • full scientific name: Clostridium tertium (Henry 1917) Bergey et al. 1923 (Approved Lists 1980)
  • synonyms

    • @ref: 20215
    • synonym: Bacillus tertius

@ref: 1094

domain: Bacteria

phylum: Firmicutes

class: Clostridia

order: Clostridiales

family: Clostridiaceae

genus: Clostridium

species: Clostridium tertium

full scientific name: Clostridium tertium (Henry 1917) Bergey et al. 1923

strain designation: 795

type strain: yes

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
1094CHOPPED MEAT MEDIUM WITH CARBOHYDRATES (DSMZ Medium 110)yeshttps://mediadive.dsmz.de/medium/110Name: CHOPPED MEAT MEDIUM WITH CARBOHYDRATES (DSMZ Medium 110) Composition: Ground beef 500.0 g/l Casitone 30.0 g/l Agar 15.0 g/l K2HPO4 5.0 g/l Yeast extract 5.0 g/l D-Glucose 4.0 g/l Starch 1.0 g/l Maltose 1.0 g/l Cellobiose 1.0 g/l L-Cysteine HCl 0.5 g/l Ethanol 0.19 g/l Vitamin K3 0.05 g/l Hemin 0.005 g/l Sodium resazurin 0.0005 g/l Vitamin K1 NaOH Distilled water
1094CHOPPED MEAT MEDIUM (DSMZ Medium 78)yeshttps://mediadive.dsmz.de/medium/78Name: CHOPPED MEAT MEDIUM (DSMZ Medium 78) Composition: Ground beef 500.0 g/l Casitone 30.0 g/l Agar 15.0 g/l Ethanol 9.5 g/l (optional) K2HPO4 5.0 g/l Yeast extract 5.0 g/l L-Cysteine HCl 0.5 g/l Haemin 0.005 g/l (optional) Resazurin 0.001 g/l Vitamin K3 0.0005 g/l (optional) Vitamin K1 (optional) NaOH (optional) Distilled water

culture temp

@refgrowthtypetemperature
1094positivegrowth37
67770positivegrowth37

Physiology and metabolism

oxygen tolerance

  • @ref: 1094
  • oxygen tolerance: anaerobe

Isolation, sampling and environmental information

taxonmaps

  • @ref: 69479
  • File name: preview.99_3264.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_34;96_1253;97_2035;98_2501;99_3264&stattab=map
  • Last taxonomy: Clostridium tertium
  • 16S sequence: Y18174
  • Sequence Identity:
  • Total samples: 7812
  • soil counts: 574
  • aquatic counts: 386
  • animal counts: 6806
  • plant counts: 46

Safety information

risk assessment

  • @ref: 1094
  • pathogenicity human: yes
  • pathogenicity animal: yes
  • biosafety level: 2
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Clostridium tertium (ATCC 14573) 16S rRNA geneAJ2454131415nuccore1559
20218Clostridium tertium strain DSM 2485 16S ribosomal RNA gene, partial sequenceHM245930842nuccore1559
20218Clostridium tertium gene for 16S ribosomal RNA, partial cds, strain: JCM 6289AB6187891477nuccore1559
1094Clostridium tertium 16S rRNA gene, partial, strain DSM 2485Y181741479nuccore1559

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Clostridium tertium DSM 2485GCA_017873245contigncbi1559
66792Clostridium tertium strain DSM 24851559.80wgspatric1559
66792Clostridium tertium DSM 24852913329792draftimg1559

GC content

@refGC-contentmethod
109428.0
6777024thermal denaturation, midpoint method (Tm)

Genome-based predictions

predictions

@reftraitmodeldescriptionpredictionconfidencetraining_data
69480gram-positivegram-positivePositive reaction to Gram-stainingyes65.98no
69480anaerobicanaerobicAbility to grow under anoxygenic conditions (including facultative anaerobes)yes84.42yes
69480spore-formingspore-formingAbility to form endo- or exosporesyes80.693no
69480aerobicaerobicAbility to grow under oxygenic conditions (including facultative aerobes)no95.099no
69480thermophilicthermophileAbility to grow at temperatures above or equal to 45°Cno94.393yes
69480flagellatedmotile2+Ability to perform flagellated movementyes76.814no

External links

@ref: 1094

culture collection no.: DSM 2485, ATCC 14573, IAM 14196, NCIB 10697, JCM 6289, CCUG 4219, NBRC 103192, NCIMB 10697, NCIMB 541

straininfo link

  • @ref: 72252
  • straininfo: 265725

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
1094Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 2485)https://www.dsmz.de/collection/catalogue/details/culture/DSM-2485
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
72252Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID265725.1StrainInfo: A central database for resolving microbial strain identifiers