Strain identifier
BacDive ID: 2716
Type strain: 
Species: Clostridium sporosphaeroides
Strain history: <- ATCC <- L. DS. Smith, Virginia Polytechnical Institute and State University, Blacksburg, USA; VPI 4527
NCBI tax ID(s): 1121334 (strain), 1549 (species)
version 8.1 (current version)
General
@ref: 625
BacDive-ID: 2716
DSM-Number: 1294
keywords: genome sequence, 16S sequence, Bacteria, anaerobe, mesophilic, Gram-positive
description: Clostridium sporosphaeroides DSM 1294 is an anaerobe, mesophilic, Gram-positive bacterium of the family Clostridiaceae.
NCBI tax id
| NCBI tax id | Matching level |
|---|---|
| 1549 | species |
| 1121334 | strain |
strain history
- @ref: 625
- history: <- ATCC <- L. DS. Smith, Virginia Polytechnical Institute and State University, Blacksburg, USA; VPI 4527
doi: 10.13145/bacdive2716.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/bacillota
- domain: Bacteria
- phylum: Bacillota
- class: Clostridia
- order: Eubacteriales
- family: Clostridiaceae
- genus: Clostridium
- species: Clostridium sporosphaeroides
- full scientific name: Clostridium sporosphaeroides Soriano and Soriano 1948 (Approved Lists 1980)
@ref: 625
domain: Bacteria
phylum: Firmicutes
class: Clostridia
order: Clostridiales
family: Clostridiaceae
genus: Clostridium
species: Clostridium sporosphaeroides
full scientific name: Clostridium sporosphaeroides Soriano and Soriano 1948
type strain: yes
Morphology
cell morphology
- @ref: 69480
- gram stain: positive
- confidence: 100
Culture and growth conditions
culture medium
- @ref: 625
- name: CHOPPED MEAT MEDIUM (DSMZ Medium 78)
- growth: yes
- link: https://mediadive.dsmz.de/medium/78
- composition: Name: CHOPPED MEAT MEDIUM (DSMZ Medium 78) Composition: Ground beef 500.0 g/l Casitone 30.0 g/l Agar 15.0 g/l Ethanol 9.5 g/l (optional) K2HPO4 5.0 g/l Yeast extract 5.0 g/l L-Cysteine HCl 0.5 g/l Haemin 0.005 g/l (optional) Resazurin 0.001 g/l Vitamin K3 0.0005 g/l (optional) Vitamin K1 (optional) NaOH (optional) Distilled water
culture temp
- @ref: 625
- growth: positive
- type: growth
- temperature: 37
- range: mesophilic
Physiology and metabolism
oxygen tolerance
| @ref | oxygen tolerance | confidence |
|---|---|---|
| 625 | anaerobe | |
| 69480 | anaerobe | 100 |
spore formation
- @ref: 69480
- spore formation: no
- confidence: 99.152
compound production
- @ref: 625
- compound: citrate lyase
Safety information
risk assessment
- @ref: 625
- biosafety level: 1
- biosafety level comment: Risk group (German classification)
Sequence information
16S sequences
| @ref | description | accession | length | database | NCBI tax ID |
|---|---|---|---|---|---|
| 20218 | C.sporosphaeroides 16S rRNA | X66002 | 1400 | ena | 1549 |
| 625 | Clostridium sporosphaeroides 16S ribosomal RNA | M59116 | 1460 | ena | 1549 |
Genome sequences
| @ref | description | accession | assembly level | database | NCBI tax ID |
|---|---|---|---|---|---|
| 66792 | [Clostridium] sporosphaeroides DSM 1294 VPI 4527 | GCA_000383295 | scaffold | ncbi | 1121334 |
| 66792 | Clostridium sporosphaeroides DSM 1294 | 1121334.3 | wgs | patric | 1121334 |
| 66792 | Clostridium sporosphaeroides DSM 1294 | 2519899647 | draft | img | 1121334 |
GC content
- @ref: 625
- GC-content: 27.0
Genome-based predictions
predictions
| trait | prediction | confidence | training_data |
|---|---|---|---|
| spore-forming | no | 80 | no |
| motile | yes | 83.596 | no |
| flagellated | no | 64.276 | no |
| gram-positive | yes | 56.45 | no |
| anaerobic | yes | 99.508 | no |
| aerobic | no | 98.744 | yes |
| halophile | no | 91.399 | no |
| spore-forming | yes | 55.251 | no |
| thermophile | no | 97.581 | yes |
| glucose-util | yes | 80.829 | no |
| glucose-ferment | yes | 59.505 | no |
External links
@ref: 625
culture collection no.: DSM 1294, ATCC 25781, NCIB 10672, VPI 4527, NCIMB 10672
straininfo link
- @ref: 72244
- straininfo: 125562
literature
| topic | Pubmed-ID | title | authors | journal | DOI | year | mesh | topic2 |
|---|---|---|---|---|---|---|---|---|
| Metabolism | 20721546 | Production of hexanoic acid from D-galactitol by a newly isolated Clostridium sp. BS-1. | Jeon BS, Kim BC, Um Y, Sang BI | Appl Microbiol Biotechnol | 10.1007/s00253-010-2827-5 | 2010 | Acetic Acid/metabolism, Anaerobiosis, Base Sequence, Biofuels, Butyric Acid/metabolism, Caproates/isolation & purification/*metabolism, Clostridium/classification/genetics/isolation & purification/*metabolism, Fermentation/genetics, Galactitol/*metabolism, Genes, rRNA, Oxidation-Reduction, Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Sequence Homology, Nucleic Acid, Sewage, Sodium Acetate/metabolism, Water Microbiology | Phylogeny |
| Phylogeny | 26474980 | Caproiciproducens galactitolivorans gen. nov., sp. nov., a bacterium capable of producing caproic acid from galactitol, isolated from a wastewater treatment plant. | Kim BC, Seung Jeon B, Kim S, Kim H, Um Y, Sang BI | Int J Syst Evol Microbiol | 10.1099/ijsem.0.000665 | 2015 | Bacterial Typing Techniques, Base Composition, Caproates/*metabolism, Clostridiales/*classification/genetics/isolation & purification, DNA, Bacterial/genetics, Fatty Acids/chemistry, Galactitol/*metabolism, Molecular Sequence Data, Nucleic Acid Hybridization, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Waste Water/*microbiology | Metabolism |
| Phylogeny | 32584751 | Caproicibacter fermentans gen. nov., sp. nov., a new caproate-producing bacterium and emended description of the genus Caproiciproducens. | Flaiz M, Baur T, Brahner S, Poehlein A, Daniel R, Bengelsdorf FR | Int J Syst Evol Microbiol | 10.1099/ijsem.0.004283 | 2020 | Bacteria, Anaerobic/classification, Bacterial Typing Techniques, Base Composition, Bioreactors/*microbiology, Caproates/*metabolism, Clostridiales/*classification/isolation & purification, DNA, Bacterial/genetics, Fatty Acids/chemistry, Fermentation, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA | Transcriptome |
Reference
| @id | authors | catalogue | doi/url | title | journal | pubmed |
|---|---|---|---|---|---|---|
| 625 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 1294) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-1294 | |||
| 20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | |||
| 20218 | Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P. | 10.1016/j.syapm.2013.11.002 | StrainInfo introduces electronic passports for microorganisms. | Syst Appl Microbiol. 37: 42-50 2014 | 24321274 | |
| 66792 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | |||
| 69480 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | |||
| 72244 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID125562.1 | StrainInfo: A central database for resolving microbial strain identifiers |