Strain identifier

BacDive ID: 2613

Type strain: Yes

Species: Hathewaya limosa

Strain Designation: A 46

Strain history: ATCC 25620 <-- W. E. C. Moore VPI 2700 <-- A. Prévot A46.

NCBI tax ID(s): 1536 (species)

For citation purpose refer to the digital object identifier (doi) of the current version.
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General

@ref: 765

BacDive-ID: 2613

DSM-Number: 1400

keywords: mesophilic, spore-forming, anaerobe, Bacteria, 16S sequence, genome sequence, Gram-positive, motile, rod-shaped, colony-forming

description: Hathewaya limosa A 46 is an anaerobe, spore-forming, mesophilic bacterium that forms irregular colonies and was isolated from mud.

NCBI tax id

  • NCBI tax id: 1536
  • Matching level: species

strain history

doi: 10.13145/bacdive2613.20220920.7

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Clostridia
  • order: Eubacteriales
  • family: Clostridiaceae
  • genus: Hathewaya
  • species: Hathewaya limosa
  • full scientific name: Hathewaya limosa (André 1948) Lawson and Rainey 2016
  • synonyms

    • @ref: 20215
    • synonym: Clostridium limosum

@ref: 765

domain: Bacteria

phylum: Firmicutes

class: Clostridia

order: Clostridiales

family: Clostridiaceae

genus: Hathewaya

species: Hathewaya limosa

full scientific name: Hathewaya limosa (André 1948) Lawson and Rainey 2016

strain designation: A 46

type strain: yes

Morphology

cell morphology

  • @ref: 43779
  • gram stain: positive
  • cell length: 1.7-16 µm
  • cell width: 0.6-1.6 µm
  • cell shape: rod-shaped
  • motility: yes
  • flagellum arrangement: peritrichous

colony morphology

  • @ref: 43779
  • colony size: 1-4 mm
  • colony color: Grey
  • colony shape: irregular
  • medium used: Blood agar plates

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
765CHOPPED MEAT MEDIUM WITH CARBOHYDRATES (DSMZ Medium 110)yeshttps://bacmedia.dsmz.de/medium/110Name: CHOPPED MEAT MEDIUM WITH CARBOHYDRATES (DSMZ Medium 110) Composition: Ground beef 500.0 g/l Casitone 30.0 g/l Agar 15.0 g/l K2HPO4 5.0 g/l Yeast extract 5.0 g/l D-Glucose 4.0 g/l Starch 1.0 g/l Maltose 1.0 g/l Cellobiose 1.0 g/l L-Cysteine HCl 0.5 g/l Ethanol 0.19 g/l Vitamin K3 0.05 g/l Hemin 0.005 g/l Sodium resazurin 0.0005 g/l NaOH Vitamin K1 Distilled water
43779Blood agar platesyes

culture temp

@refgrowthtypetemperaturerange
765positivegrowth37mesophilic
43779positiveoptimum37mesophilic
67770positivegrowth37mesophilic

Physiology and metabolism

oxygen tolerance

  • @ref: 765
  • oxygen tolerance: anaerobe

spore formation

  • @ref: 43779
  • spore description: Oval, central or subterminal, usually swell the cell
  • type of spore: spore
  • spore formation: yes

halophily

  • @ref: 43779
  • salt: NaCl
  • growth: no
  • tested relation: other
  • concentration: 6.5 %(w/v)

observation

  • @ref: 43779
  • observation: Supernatant culture fluids of some strains are weakly toxic to mice; some strains are pathogenic for guinea pigs

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
4377924996lactate-assimilation
4377926986threonine-assimilation
4377927570histidine+fermentation

antibiotic resistance

@refChEBImetaboliteis antibioticis sensitiveis resistant
4377917698chloramphenicolyesyes
4377948923erythromycinyesyes
4377918208penicillin gyesyes
4377927902tetracyclineyesyes
437793745clindamycinyesyes

metabolite production

@refChebi-IDmetaboliteproduction
4377916134ammoniayes
4377918397beta-phenylethylamineyes
4377916136hydrogen sulfideyes
4377935581indoleyes
4377915882phenolyes

enzymes

@refvalueactivity
43779lecithinase+
43779DNase+

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountrycontinentorigin.country
765mudEbrie Laguna
43779mud; infections in cattle, water buffalo, alligators and chickens; snake venom; home-preserved meat; human feaces; human clinical specimen including blood, peritoneal fluid, pleural fluids, and lung biopsy from pulmonary infections
47908MudIvory coast,Ebrie Laguna,AfricaAfrica
67770Mud from Ebrie lagunaCôte d'IvoireAfricaCIV

isolation source categories

Cat1Cat2Cat3
#Environmental#Terrestrial#Mud (Sludge)
#Host#Human
#Host#Mammals#Bovinae (Cow, Cattle)
#Host#Mammals#Muridae (Mouse/Rat)
#Host Body Product#Gastrointestinal tract#Feces (Stool)
#Infection#Disease

Safety information

risk assessment

  • @ref: 765
  • biosafety level: 2
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
765Clostridium limosum partial 16S rRNA gene, type strain CECT 4329TFR8704441500ena1536
67770Clostridium limosum gene for 16S ribosomal RNA, partial sequence, strain: JCM 1427LC0363181476ena1536

Genome sequences

  • @ref: 66792
  • description: Hathewaya limosa DSM 1400
  • accession: 2928998743
  • assembly level: draft
  • database: img
  • NCBI tax ID: 1536

GC content

@refGC-contentmethod
76524.0thermal denaturation, midpoint method (Tm)
4377924thermal denaturation, midpoint method (Tm)

External links

@ref: 765

culture collection no.: DSM 1400, ATCC 25620, ATCC 25760, NCIB 10638, CCUG 24037, VPI 2700, JCM 1427, BCRC 14513, CECT 4329, NCIMB 10638

straininfo link

@refpassport
20218http://www.straininfo.net/strains/132038
20218http://www.straininfo.net/strains/104413
20218http://www.straininfo.net/strains/104416
20218http://www.straininfo.net/strains/132039

literature

  • topic: Phylogeny
  • Pubmed-ID: 23811141
  • title: Oceanirhabdus sediminicola gen. nov., sp. nov., an anaerobic bacterium isolated from sea sediment.
  • authors: Pi RX, Zhang WW, Fang MX, Zhang YZ, Li TT, Wu M, Zhu XF
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/ijs.0.051243-0
  • year: 2013
  • mesh: Bacteria, Anaerobic/*classification/genetics/isolation & purification, Bacterial Typing Techniques, Base Composition, China, DNA, Bacterial/genetics, Fatty Acids/chemistry, Geologic Sediments/*microbiology, Glycolipids/chemistry, Gram-Positive Endospore-Forming Rods/*classification/genetics/isolation & purification, Molecular Sequence Data, *Phylogeny, Quinones/chemistry, RNA, Ribosomal, 16S/genetics, Seawater/*microbiology, Sequence Analysis, DNA
  • topic2: Enzymology

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
765Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 1400)https://www.dsmz.de/collection/catalogue/details/culture/DSM-1400
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
43779Paul A. Lawson, Fred A. Rainey10.1099/ijsem.0.000824Proposal to restrict the genus Clostridium Prazmowski to Clostridium butyricum and related speciesIJSEM 66: 1009-1016 201626643615
47908Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 24037)https://www.ccug.se/strain?id=24037
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/