Strain identifier

BacDive ID: 2613

Type strain: Yes

Species: Hathewaya limosa

Strain Designation: A 46

Strain history: ATCC 25620 <-- W. E. C. Moore VPI 2700 <-- A. Prévot A46.

NCBI tax ID(s): 1536 (species)

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@ref: 765

BacDive-ID: 2613

DSM-Number: 1400

keywords: mesophilic, spore-forming, anaerobe, Bacteria, 16S sequence, genome sequence, Gram-positive, motile, rod-shaped, colony-forming

description: Hathewaya limosa A 46 is an anaerobe, spore-forming, mesophilic bacterium that forms irregular colonies and was isolated from mud.

NCBI tax id

  • NCBI tax id: 1536
  • Matching level: species

strain history

doi: 10.13145/bacdive2613.20220920.7

Name and taxonomic classification


  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Clostridia
  • order: Eubacteriales
  • family: Clostridiaceae
  • genus: Hathewaya
  • species: Hathewaya limosa
  • full scientific name: Hathewaya limosa (André 1948) Lawson and Rainey 2016
  • synonyms

    • @ref: 20215
    • synonym: Clostridium limosum

@ref: 765

domain: Bacteria

phylum: Firmicutes

class: Clostridia

order: Clostridiales

family: Clostridiaceae

genus: Hathewaya

species: Hathewaya limosa

full scientific name: Hathewaya limosa (André 1948) Lawson and Rainey 2016

strain designation: A 46

type strain: yes


cell morphology

  • @ref: 43779
  • gram stain: positive
  • cell length: 1.7-16 µm
  • cell width: 0.6-1.6 µm
  • cell shape: rod-shaped
  • motility: yes
  • flagellum arrangement: peritrichous

colony morphology

  • @ref: 43779
  • colony size: 1-4 mm
  • colony color: Grey
  • colony shape: irregular
  • medium used: Blood agar plates

Culture and growth conditions

culture medium

765CHOPPED MEAT MEDIUM WITH CARBOHYDRATES (DSMZ Medium 110)yes CHOPPED MEAT MEDIUM WITH CARBOHYDRATES (DSMZ Medium 110) Composition: Ground beef 500.0 g/l Casitone 30.0 g/l Agar 15.0 g/l K2HPO4 5.0 g/l Yeast extract 5.0 g/l D-Glucose 4.0 g/l Starch 1.0 g/l Maltose 1.0 g/l Cellobiose 1.0 g/l L-Cysteine HCl 0.5 g/l Ethanol 0.19 g/l Vitamin K3 0.05 g/l Hemin 0.005 g/l Sodium resazurin 0.0005 g/l NaOH Vitamin K1 Distilled water
43779Blood agar platesyes

culture temp


Physiology and metabolism

oxygen tolerance

  • @ref: 765
  • oxygen tolerance: anaerobe

spore formation

  • @ref: 43779
  • spore description: Oval, central or subterminal, usually swell the cell
  • type of spore: spore
  • spore formation: yes


  • @ref: 43779
  • salt: NaCl
  • growth: no
  • tested relation: other
  • concentration: 6.5 %(w/v)


  • @ref: 43779
  • observation: Supernatant culture fluids of some strains are weakly toxic to mice; some strains are pathogenic for guinea pigs

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested

antibiotic resistance

@refChEBImetaboliteis antibioticis sensitiveis resistant
4377918208penicillin gyesyes

metabolite production

4377916136hydrogen sulfideyes



Isolation, sampling and environmental information


@refsample typegeographic
765mudEbrie Laguna
43779mud; infections in cattle, water buffalo, alligators and chickens; snake venom; home-preserved meat; human feaces; human clinical specimen including blood, peritoneal fluid, pleural fluids, and lung biopsy from pulmonary infections
47908MudIvory coast,Ebrie Laguna,AfricaAfrica
67770Mud from Ebrie lagunaCôte d'IvoireAfricaCIV

isolation source categories

#Environmental#Terrestrial#Mud (Sludge)
#Host#Mammals#Bovinae (Cow, Cattle)
#Host#Mammals#Muridae (Mouse/Rat)
#Host Body Product#Gastrointestinal tract#Feces (Stool)

Safety information

risk assessment

  • @ref: 765
  • biosafety level: 2
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
765Clostridium limosum partial 16S rRNA gene, type strain CECT 4329TFR8704441500ena1536
67770Clostridium limosum gene for 16S ribosomal RNA, partial sequence, strain: JCM 1427LC0363181476ena1536

Genome sequences

  • @ref: 66792
  • description: Hathewaya limosa DSM 1400
  • accession: 2928998743
  • assembly level: draft
  • database: img
  • NCBI tax ID: 1536

GC content

76524.0thermal denaturation, midpoint method (Tm)
4377924thermal denaturation, midpoint method (Tm)

External links

@ref: 765

culture collection no.: DSM 1400, ATCC 25620, ATCC 25760, NCIB 10638, CCUG 24037, VPI 2700, JCM 1427, BCRC 14513, CECT 4329, NCIMB 10638

straininfo link



  • topic: Phylogeny
  • Pubmed-ID: 23811141
  • title: Oceanirhabdus sediminicola gen. nov., sp. nov., an anaerobic bacterium isolated from sea sediment.
  • authors: Pi RX, Zhang WW, Fang MX, Zhang YZ, Li TT, Wu M, Zhu XF
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/ijs.0.051243-0
  • year: 2013
  • mesh: Bacteria, Anaerobic/*classification/genetics/isolation & purification, Bacterial Typing Techniques, Base Composition, China, DNA, Bacterial/genetics, Fatty Acids/chemistry, Geologic Sediments/*microbiology, Glycolipids/chemistry, Gram-Positive Endospore-Forming Rods/*classification/genetics/isolation & purification, Molecular Sequence Data, *Phylogeny, Quinones/chemistry, RNA, Ribosomal, 16S/genetics, Seawater/*microbiology, Sequence Analysis, DNA
  • topic2: Enzymology


765Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 1400)
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
43779Paul A. Lawson, Fred A. Rainey10.1099/ijsem.0.000824Proposal to restrict the genus Clostridium Prazmowski to Clostridium butyricum and related speciesIJSEM 66: 1009-1016 201626643615
47908Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 24037)
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
67770Curators of the JCM