Strain identifier
BacDive ID: 2613
Type strain: 
Species: Hathewaya limosa
Strain Designation: A 46
Strain history: ATCC 25620 <-- W. E. C. Moore VPI 2700 <-- A. Prévot A46.
NCBI tax ID(s): 1536 (species)
version 8.1 (current version)
General
@ref: 765
BacDive-ID: 2613
DSM-Number: 1400
keywords: genome sequence, 16S sequence, Bacteria, anaerobe, spore-forming, mesophilic, Gram-positive, motile, rod-shaped, colony-forming
description: Hathewaya limosa A 46 is an anaerobe, spore-forming, mesophilic bacterium that forms irregular colonies and was isolated from mud.
NCBI tax id
- NCBI tax id: 1536
- Matching level: species
strain history
| @ref | history |
|---|---|
| 765 | <- ATCC <- W.E.C. Moore, VPI 2700 <- A.R. Prévot, A 46 |
| 67770 | ATCC 25620 <-- W. E. C. Moore VPI 2700 <-- A. Prévot A46. |
doi: 10.13145/bacdive2613.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/bacillota
- domain: Bacteria
- phylum: Bacillota
- class: Clostridia
- order: Eubacteriales
- family: Clostridiaceae
- genus: Hathewaya
- species: Hathewaya limosa
- full scientific name: Hathewaya limosa (André 1948) Lawson and Rainey 2016
synonyms
- @ref: 20215
- synonym: Clostridium limosum
@ref: 765
domain: Bacteria
phylum: Firmicutes
class: Clostridia
order: Clostridiales
family: Clostridiaceae
genus: Hathewaya
species: Hathewaya limosa
full scientific name: Hathewaya limosa (André 1948) Lawson and Rainey 2016
strain designation: A 46
type strain: yes
Morphology
cell morphology
- @ref: 43779
- gram stain: positive
- cell length: 1.7-16 µm
- cell width: 0.6-1.6 µm
- cell shape: rod-shaped
- motility: yes
- flagellum arrangement: peritrichous
colony morphology
- @ref: 43779
- colony size: 1-4 mm
- colony color: Grey
- colony shape: irregular
- medium used: Blood agar plates
Culture and growth conditions
culture medium
| @ref | name | growth | link | composition |
|---|---|---|---|---|
| 765 | CHOPPED MEAT MEDIUM WITH CARBOHYDRATES (DSMZ Medium 110) | yes | https://mediadive.dsmz.de/medium/110 | Name: CHOPPED MEAT MEDIUM WITH CARBOHYDRATES (DSMZ Medium 110) Composition: Ground beef 500.0 g/l Casitone 30.0 g/l Agar 15.0 g/l K2HPO4 5.0 g/l Yeast extract 5.0 g/l D-Glucose 4.0 g/l Starch 1.0 g/l Maltose 1.0 g/l Cellobiose 1.0 g/l L-Cysteine HCl 0.5 g/l Ethanol 0.19 g/l Vitamin K3 0.05 g/l Hemin 0.005 g/l Sodium resazurin 0.0005 g/l Vitamin K1 NaOH Distilled water |
| 43779 | Blood agar plates | yes |
culture temp
| @ref | growth | type | temperature | range |
|---|---|---|---|---|
| 765 | positive | growth | 37 | mesophilic |
| 43779 | positive | optimum | 37 | mesophilic |
| 67770 | positive | growth | 37 | mesophilic |
Physiology and metabolism
oxygen tolerance
- @ref: 765
- oxygen tolerance: anaerobe
spore formation
- @ref: 43779
- spore description: Oval, central or subterminal, usually swell the cell
- type of spore: spore
- spore formation: yes
halophily
- @ref: 43779
- salt: NaCl
- growth: no
- tested relation: other
- concentration: 6.5 %(w/v)
observation
- @ref: 43779
- observation: Supernatant culture fluids of some strains are weakly toxic to mice; some strains are pathogenic for guinea pigs
metabolite utilization
| @ref | Chebi-ID | metabolite | utilization activity | kind of utilization tested |
|---|---|---|---|---|
| 43779 | 24996 | lactate | - | assimilation |
| 43779 | 26986 | threonine | - | assimilation |
| 43779 | 27570 | histidine | + | fermentation |
antibiotic resistance
| @ref | ChEBI | metabolite | is antibiotic | is sensitive | is resistant |
|---|---|---|---|---|---|
| 43779 | 17698 | chloramphenicol | yes | yes | |
| 43779 | 48923 | erythromycin | yes | yes | |
| 43779 | 18208 | penicillin g | yes | yes | |
| 43779 | 27902 | tetracycline | yes | yes | |
| 43779 | 3745 | clindamycin | yes | yes |
metabolite production
| @ref | Chebi-ID | metabolite | production |
|---|---|---|---|
| 43779 | 16134 | ammonia | yes |
| 43779 | 18397 | beta-phenylethylamine | yes |
| 43779 | 16136 | hydrogen sulfide | yes |
| 43779 | 35581 | indole | yes |
| 43779 | 15882 | phenol | yes |
enzymes
| @ref | value | activity |
|---|---|---|
| 43779 | lecithinase | + |
| 43779 | DNase | + |
Isolation, sampling and environmental information
isolation
| @ref | sample type | geographic location | country | continent | origin.country |
|---|---|---|---|---|---|
| 765 | mud | Ebrie Laguna | |||
| 43779 | mud; infections in cattle, water buffalo, alligators and chickens; snake venom; home-preserved meat; human feaces; human clinical specimen including blood, peritoneal fluid, pleural fluids, and lung biopsy from pulmonary infections | ||||
| 47908 | Mud | Ivory coast,Ebrie Laguna, | Africa | Africa | |
| 67770 | Mud from Ebrie laguna | Côte d'Ivoire | Africa | CIV |
isolation source categories
| Cat1 | Cat2 | Cat3 |
|---|---|---|
| #Environmental | #Terrestrial | #Mud (Sludge) |
| #Host | #Human | |
| #Host | #Mammals | #Bovinae (Cow, Cattle) |
| #Host | #Mammals | #Muridae (Mouse/Rat) |
| #Host Body Product | #Gastrointestinal tract | #Feces (Stool) |
| #Infection | #Disease |
taxonmaps
- @ref: 69479
- File name: preview.99_101021.png
- url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_34;96_10839;97_13127;98_69018;99_101021&stattab=map
- Last taxonomy: Hathewaya limosa subclade
- 16S sequence: LC036318
- Sequence Identity:
- Total samples: 4824
- soil counts: 886
- aquatic counts: 1310
- animal counts: 2557
- plant counts: 71
Safety information
risk assessment
- @ref: 765
- biosafety level: 2
- biosafety level comment: Risk group (German classification)
Sequence information
16S sequences
| @ref | description | accession | length | database | NCBI tax ID |
|---|---|---|---|---|---|
| 765 | Clostridium limosum partial 16S rRNA gene, type strain CECT 4329T | FR870444 | 1500 | ena | 1536 |
| 67770 | Clostridium limosum gene for 16S ribosomal RNA, partial sequence, strain: JCM 1427 | LC036318 | 1476 | ena | 1536 |
Genome sequences
- @ref: 66792
- description: Hathewaya limosa DSM 1400
- accession: 2928998743
- assembly level: draft
- database: img
- NCBI tax ID: 1536
GC content
| @ref | GC-content | method |
|---|---|---|
| 765 | 24.0 | thermal denaturation, midpoint method (Tm) |
| 43779 | 24 | thermal denaturation, midpoint method (Tm) |
External links
@ref: 765
culture collection no.: DSM 1400, ATCC 25620, ATCC 25760, NCIB 10638, CCUG 24037, VPI 2700, JCM 1427, BCRC 14513, CECT 4329, NCIMB 10638
straininfo link
- @ref: 72144
- straininfo: 38620
literature
- topic: Phylogeny
- Pubmed-ID: 23811141
- title: Oceanirhabdus sediminicola gen. nov., sp. nov., an anaerobic bacterium isolated from sea sediment.
- authors: Pi RX, Zhang WW, Fang MX, Zhang YZ, Li TT, Wu M, Zhu XF
- journal: Int J Syst Evol Microbiol
- DOI: 10.1099/ijs.0.051243-0
- year: 2013
- mesh: Bacteria, Anaerobic/*classification/genetics/isolation & purification, Bacterial Typing Techniques, Base Composition, China, DNA, Bacterial/genetics, Fatty Acids/chemistry, Geologic Sediments/*microbiology, Glycolipids/chemistry, Gram-Positive Endospore-Forming Rods/*classification/genetics/isolation & purification, Molecular Sequence Data, *Phylogeny, Quinones/chemistry, RNA, Ribosomal, 16S/genetics, Seawater/*microbiology, Sequence Analysis, DNA
- topic2: Enzymology
Reference
| @id | authors | catalogue | doi/url | title | journal | pubmed |
|---|---|---|---|---|---|---|
| 765 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 1400) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-1400 | |||
| 20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | |||
| 43779 | Paul A. Lawson, Fred A. Rainey | 10.1099/ijsem.0.000824 | Proposal to restrict the genus Clostridium Prazmowski to Clostridium butyricum and related species | IJSEM 66: 1009-1016 2016 | 26643615 | |
| 47908 | Curators of the CCUG | Culture Collection University of Gothenburg (CCUG) (CCUG 24037) | https://www.ccug.se/strain?id=24037 | |||
| 66792 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | |||
| 67770 | Curators of the JCM | https://jcm.brc.riken.jp/en/ | ||||
| 69479 | João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich. | https://microbeatlas.org/ | MicrobeAtlas 1.0 beta | |||
| 72144 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID38620.1 | StrainInfo: A central database for resolving microbial strain identifiers |