Name: Hathewaya limosa A 46
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 2613

Type strain:

Species: Hathewaya limosa

Strain Designation: A 46

Culture col. no.: DSM 1400, ATCC 25620, ATCC 25760, NCIB 10638, CCUG 24037, VPI 2700, JCM 1427, BCRC 14513, CECT 4329, NCIMB 10638

Strain history: ATCC 25620 <-- W. E. C. Moore VPI 2700 <-- A. Prévot A46.

NCBI tax ID(s): 1536 (species)

SI-ID 38620

ATCC 25620, ATCC 25760, CCUG 24037, NCIB 10638, JCM 1427, CCRC 14513, NCIMB 10638, CECT 4329, BCRC 14513, DSM 1400, CECT 8345

Other strains from Hathewaya limosa

H. limosa CCUG 54482
H. limosa CCUG 70674

Section

Hathewaya limosa A 46 is an anaerobe, spore-forming, mesophilic bacterium that forms irregular colonies and was isolated from mud.

colony-forming
Gram-positive
motile
rod-shaped
spore-forming
anaerobe
mesophilic
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Bacillota*
	Class	*Clostridia*
	Order	*Eubacteriales*
	Family	*Clostridiaceae*
	Genus	*Hathewaya*
	Species	**Hathewaya limosa**
	Full Scientific Name (LPSN)	Hathewaya limosa (André 1948) Lawson and Rainey 2016

Synonym

Clostridium limosum

Information on morphological and physiological properties Morphology

[Ref.: #43779]	Gram stain	positive

[Ref.: #43779]	Cell length	1.7-16 µm

[Ref.: #43779]	Cell width	0.6-1.6 µm

[Ref.: #43779]	Cell shape	rod-shaped

[Ref.: #43779]	Motility	yes

[Ref.: #43779]	Flagellum arrangement	peritrichous

[Ref.: #43779]	Colony size	1-4 mm
[Ref.: #43779]	Colony color	Grey
[Ref.: #43779]	Colony shape	irregular
[Ref.: #43779]	Cultivation medium used	Blood agar plates

Information on culture and growth conditions Culture and growth conditions

[Ref.: #765]

Culture medium

CHOPPED MEAT MEDIUM WITH CARBOHYDRATES (DSMZ Medium 110)

[Ref.: #765]

Culture medium growth

[Ref.: #765]

Culture medium link

Medium recipe at MediaDive

[Ref.: #765]

Culture medium composition

expand / minimize

[Ref.: #43779]

Culture medium

Blood agar plates

[Ref.: #43779]

Culture medium growth

		Temperature
[Ref.: #765]	growth	37 °C
[Ref.: #43779]	optimum	37 °C
[Ref.: #67770]	growth	37 °C

[Ref.: #765]	Temperature range	mesophilic
[Ref.: #43779]	Temperature range	mesophilic
[Ref.: #67770]	Temperature range	mesophilic

Information on physiology and metabolism Physiology and metabolism

[Ref.: #765]

Oxygen tolerance

anaerobe

[Ref.: #43779]	Spore description	Oval, central or subterminal, usually swell the cell
[Ref.: #43779]	Type of spore	spore
[Ref.: #43779]	Ability of spore formation	yes

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #43779]		NaCl	no	other	6.5	%(w/v)

[Ref.: #43779]

Observation

Supernatant culture fluids of some strains are weakly toxic to mice; some strains are pathogenic for guinea pigs

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #43779]	histidine ChEBI	+	fermentation
[Ref.: #43779]	lactate ChEBI	-	assimilation
[Ref.: #43779]	threonine ChEBI	-	assimilation

	Metabolite	Antibiotic sensitivity	Antibiotic resistance
[Ref.: #43779]	chloramphenicol ChEBI	yes
[Ref.: #43779]	clindamycin ChEBI		yes
[Ref.: #43779]	erythromycin ChEBI	yes
[Ref.: #43779]	penicillin g ChEBI	yes
[Ref.: #43779]	tetracycline ChEBI	yes

	Metabolite	Production
[Ref.: #43779]	ammonia ChEBI	yes
[Ref.: #43779]	beta-phenylethylamine ChEBI	yes
[Ref.: #43779]	hydrogen sulfide ChEBI	yes
[Ref.: #43779]	indole ChEBI	yes
[Ref.: #43779]	phenol ChEBI	yes

	Enzymes	Enzyme	Enzyme activity
[Ref.: #43779]		DNase	+
[Ref.: #43779]		lecithinase	+

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #765]	Sample type/isolated from	mud
[Ref.: #765]	Geographic location (country and/or sea, region)	Ebrie Laguna

[Ref.: #43779]	Sample type/isolated from	mud; infections in cattle, water buffalo, alligators and chickens; snake venom; home-preserved meat; human feaces; human clinical specimen including blood, peritoneal fluid, pleural fluids, and lung biopsy from pulmonary infections

[Ref.: #47908]	Sample type/isolated from	Mud
[Ref.: #47908]	Geographic location (country and/or sea, region)	Ivory coast,Ebrie Laguna,
[Ref.: #47908]	Country	Africa
[Ref.: #47908]	Continent	Africa

[Ref.: #67770]	Sample type/isolated from	Mud from Ebrie laguna
[Ref.: #67770]	Country	Côte d'Ivoire
[Ref.: #67770]	Country ISO 3 Code	CIV
[Ref.: #67770]	Continent	Africa
* marker position based on {}

Isolation sources categories

#Environmental	#Terrestrial	#Mud (Sludge)
#Host	#Human	-
#Host	#Mammals	#Bovinae (Cow, Cattle)
#Host	#Mammals	#Muridae (Mouse/Rat)
#Host Body Product	#Gastrointestinal tract	#Feces (Stool)
#Infection	#Disease	-

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence LC036318 (>99% sequence identity) for Hathewaya limosa subclade from Microbeatlas

Aquatic Samples	1310
Soil Samples	886
Animal Samples	2557
Plant Samples	71
Total Samples	4824

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #765]

Biosafety level

Risk group (German classification)
According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #765]	Clostridium limosum partial 16S rRNA gene, type strain CECT 4329T	FR870444	1500	ENA	1536 tax ID
[Ref.: #67770]	Clostridium limosum gene for 16S ribosomal RNA, partial sequence, strain: JCM 1427	LC036318	1476	ENA	1536 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Hathewaya limosa DSM 1400	2928998743	draft	JGI IMG/M	1536 tax ID	*

[Ref.: #765]	GC-content	24.0 mol%	thermal denaturation, midpoint method (Tm)
[Ref.: #43779]	GC-content	24 mol%	thermal denaturation, midpoint method (Tm)

Availability in culture collections External links

[Ref.: #765]

Culture collection no.

DSM 1400, ATCC 25620, ATCC 25760, NCIB 10638, CCUG 24037, VPI 2700, JCM 1427, BCRC 14513, CECT 4329, NCIMB 10638

[Ref.: #72144]

SI-ID 38620

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Oceanirhabdus sediminicola gen. nov., sp. nov., an anaerobic bacterium isolated from sea sediment.	Pi RX, Zhang WW, Fang MX, Zhang YZ, Li TT, Wu M, Zhu XF	Int J Syst Evol Microbiol	10.1099/ijs.0.051243-0	2013	*

References

#765	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 1400

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#43779

Paul A. Lawson, Fred A. Rainey: Proposal to restrict the genus Clostridium Prazmowski to Clostridium butyricum and related species. IJSEM 66: 1009 - 1016 2016 ( DOI 10.1099/ijsem.0.000824 , PubMed 26643615 )

#47908 Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 24037

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#72144

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID38620.1 )

* These data were automatically processed and therefore are not curated

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