Strain identifier

BacDive ID: 26

Type strain: Yes

Species: Acidiphilium cryptum

Strain Designation: Lhet2

Strain history: IAM 14719 <-- IFO 14242 <-- A. P. Harrison, Jr. strain Lhet2.

NCBI tax ID(s): 524 (species)

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General

@ref: 1068

BacDive-ID: 26

DSM-Number: 2389

keywords: 16S sequence, Bacteria, aerobe, heterotroph, thermophilic, Gram-negative, motile, rod-shaped

description: Acidiphilium cryptum Lhet2 is an aerobe, heterotroph, thermophilic bacterium that was isolated from coal mine water.

NCBI tax id

  • NCBI tax id: 524
  • Matching level: species

strain history

@refhistory
1068<- A.P. Harrison, Jr., Lhet2 (Isolated from culture of Thiobacillus ferrooxidans) <- P.R. Dugan <- D.G. Lundgren <- W.W. Leathen
67770IAM 14719 <-- IFO 14242 <-- A. P. Harrison, Jr. strain Lhet2.

doi: 10.13145/bacdive26.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Alphaproteobacteria
  • order: Rhodospirillales
  • family: Acetobacteraceae
  • genus: Acidiphilium
  • species: Acidiphilium cryptum
  • full scientific name: Acidiphilium cryptum Harrison 1981

@ref: 1068

domain: Bacteria

phylum: Proteobacteria

class: Alphaproteobacteria

order: Rhizobiales

family: Acetobacteraceae

genus: Acidiphilium

species: Acidiphilium cryptum

full scientific name: Acidiphilium cryptum Harrison 1981

strain designation: Lhet2

type strain: yes

Morphology

cell morphology

@refgram staincell lengthcell widthcell shapemotilityflagellum arrangement
23239negative0.6-0.8 µm0.3-0.4 µmrod-shapedyesmonotrichous, polar
23239negative0.6-0.8 µm0.3-0.4 µmrod-shapedyeslophotrichous

colony morphology

@refcolony colorincubation periodmedium usedcolony size
23239translucent2-3 daysTS-glucose
232395-7 daysTS-glucose0.2-0.5 mm
23239opaque, white or pinkish whiteTS-glucose

Culture and growth conditions

culture medium

@refnamegrowthcompositionlink
23239TS-glucoseyesper liter: (NH4)2SO4, 2 g; KCl, 0,1 g; K2HPO4, 0.5 g; MgSO4.7H20,0.5 g; dehydrated Trypticase soy broth (BBL Microbiology Systems, Cockeysville, Md.), 0.1 g; and glucose, 1 g. pH adjusted to 3 with 1 N H2S04
1068ACIDIPHILIUM MEDIUM (DSMZ Medium 269)yesName: ACIDIPHILIUM MEDIUM (DSMZ Medium 269) Composition: (NH4)2SO4 2.0 g/l D-Glucose 1.0 g/l MgSO4 x 7 H2O 0.5 g/l K2HPO4 0.5 g/l Yeast extract 0.3 g/l KCl 0.1 g/l Distilled waterhttps://mediadive.dsmz.de/medium/269

culture temp

@refgrowthtypetemperaturerange
23239nogrowth47thermophilic
23239positiveoptimum35-41
23239mesophilic
1068positivegrowth28mesophilic
67770positivegrowth27mesophilic

culture pH

@refabilitytypepHPH range
23239positivegrowth1.9-5.9acidophile
23239nogrowth6.1

Physiology and metabolism

oxygen tolerance

  • @ref: 23239
  • oxygen tolerance: aerobe

nutrition type

  • @ref: 23239
  • type: heterotroph

spore formation

  • @ref: 23239
  • spore formation: no

halophily

@refsaltgrowthtested relationconcentration
23239NaClnogrowth7 %
23239NaClpositivegrowth3 %

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
2323929985L-glutamate-growth
2323915356cysteine-growth
2323915428glycine-growth
2323924996lactate-growth
2323916094thiosulfate-growth
2323922599arabinose+growth
2323917234glucose+growth
2323917754glycerol+growth
2323917196L-asparagine+growth
2323930924L-tartrate+growth
2323917716lactose+growth
2323917306maltose+growth
2323929864mannitol+growth
2323916634raffinose+growth
2323933942ribose+growth
2323917992sucrose+growth

antibiotic resistance

@refChEBImetaboliteis antibioticis resistantis sensitive
2323948923erythromycinyesyes
232397507neomycinyesyes
2323917334penicillinyesyes
2323917076streptomycinyesyes
2323917698chloramphenicolyesyes
2323927644chlortetracyclinyesyes

enzymes

@refvalueactivityec
23239catalase+/-1.11.1.6
23239cytochrome oxidase+/-1.9.3.1

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinenthost species
1068coal mine waterPennsylvaniaUSAUSANorth America
67770Culture of Thiobacillus ferrooxidans, originally grown by W. W. Leathen from coal mine waterPAUSAUSANorth AmericaThiobacillus ferrooxidans

isolation source categories

Cat1Cat2Cat3
#Engineered#Other#Mine
#Environmental#Aquatic
#Environmental#Terrestrial#Geologic

taxonmaps

  • @ref: 69479
  • File name: preview.99_618.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_29;96_391;97_440;98_502;99_618&stattab=map
  • Last taxonomy: Acidiphilium
  • 16S sequence: D30773
  • Sequence Identity:
  • Total samples: 4605
  • soil counts: 1654
  • aquatic counts: 1984
  • animal counts: 607
  • plant counts: 360

Safety information

risk assessment

  • @ref: 1068
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Acidiphilium cryptum 16S rRNA sequenceM79366315ena524
20218Acidiphilium cryptum 16S rRNA sequenceM79367350ena524
20218Acidiphilium cryptum 16S rRNA sequenceM79368303ena524
20218Acidiphilium cryptum 16S rRNA gene, partial, strain DSM 2389Y184451337ena524
1068Acidiphilium cryptum gene for 16S ribosomal RNAD307731413ena524

GC content

@refGC-contentmethod
2323969.80thermal denaturation, midpoint method (Tm)
2323969.40Buoyant density centrifugation (BD)
106869.8

External links

@ref: 1068

culture collection no.: DSM 2389, ATCC 33463, JCM 21277, IAM 14719, IFO 14242, NBRC 14242, NCIMB 11690

straininfo link

  • @ref: 69710
  • straininfo: 47606

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Metabolism16346876Glucose catabolism in strains of acidophilic, heterotrophic bacteria.Shuttleworth KL, Unz RF, Wichlacz PLAppl Environ Microbiol10.1128/aem.50.3.573-579.19851985
Metabolism19712391Iron respiration by Acidiphilium cryptum at pH 5.Bilgin AA, Silverstein J, Jenkins JDFEMS Microbiol Ecol10.1016/j.femsec.2003.08.0182004Acidiphilium/growth & development/*metabolism, Aerobiosis, Ferric Compounds/*metabolism, Hydrogen-Ion Concentration
Metabolism25142158The hydroxyectoine gene cluster of the non-halophilic acidophile Acidiphilium cryptum.Moritz KD, Amendt B, Witt EM, Galinski EAExtremophiles10.1007/s00792-014-0687-02014Acidiphilium/*genetics, Alum Compounds/chemistry, Amino Acids, Diamino/*biosynthesis, Computational Biology, Gene Expression Regulation, Bacterial, Genes, Bacterial, Genetic Vectors, Hydro-Lyases/metabolism, Hydrogen-Ion Concentration, Isoelectric Point, *Multigene Family, Osmotic Pressure, Sodium Chloride/chemistry
Metabolism32699914Boosting Escherichia coli's heterologous production rate of ectoines by exploiting the non-halophilic gene cluster from Acidiphilium cryptum.Bethlehem L, Moritz KDExtremophiles10.1007/s00792-020-01188-82020Acidiphilium/genetics, *Amino Acids, Diamino/metabolism, *Escherichia coli/metabolism, Multigene FamilyBiotechnology

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
1068Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 2389)https://www.dsmz.de/collection/catalogue/details/culture/DSM-2389
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
23239Arthur P., Harrison JR.10.1099/00207713-31-3-327Acidiphilium cryptum gen. nov., sp. nov., Heterotrophic Bacterium From Acidic Mineral EnvironmentsIJSEM 31: 327-332 1981
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
69710Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID47606.1StrainInfo: A central database for resolving microbial strain identifiers