Strain identifier

BacDive ID: 26

Type strain: Yes

Species: Acidiphilium cryptum

Strain Designation: Lhet2

Culture col. no.: DSM 2389, ATCC 33463

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General

@ref: 1068

BacDive-ID: 26

DSM-Number: 2389

keywords: motile, gram-negative, thermophilic, heterotroph, aerobe, Bacteria, 16S sequence, rod-shaped

description: Acidiphilium cryptum Lhet2 is an aerobe, heterotroph, thermophilic bacterium that was isolated from coal mine water.

strain history: <- A.P. Harrison, Jr., Lhet2 (Isolated from culture of Thiobacillus ferrooxidans) <- P.R. Dugan <- D.G. Lundgren <- W.W. Leathen

doi: 10.13145/bacdive26.20201210.5

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/proteobacteria
  • domain: Bacteria
  • phylum: Proteobacteria
  • class: Alphaproteobacteria
  • order: Rhodospirillales
  • family: Acetobacteraceae
  • genus: Acidiphilium
  • species: Acidiphilium cryptum
  • full scientific name: Acidiphilium cryptum Harrison 1981

@ref: 1068

domain: Bacteria

phylum: Proteobacteria

class: Alphaproteobacteria

order: Rhizobiales

family: Acetobacteraceae

genus: Acidiphilium

species: Acidiphilium cryptum

full scientific name: Acidiphilium cryptum Harrison 1981

strain designation: Lhet2

type strain: yes

Morphology

cell morphology

@refgram staincell lengthcell widthcell shapemotilityflagellum arrangement
23239negative0.6-0.8 µm0.3-0.4 µmrod-shapedyesmonotrichous, polar
23239negative0.6-0.8 µm0.3-0.4 µmrod-shapedyeslophotrichous

colony morphology

@refcolony colorincubation periodmedium usedcolony size
23239translucent2-3 daysTS-glucose
232395-7 daysTS-glucose0.2-0.5 mm
23239opaque, white or pinkish whiteTS-glucose

Culture and growth conditions

culture medium

@refnamegrowthcompositionlink
23239TS-glucoseyesper liter: (NH4)2SO4, 2 g; KCl, 0,1 g; K2HPO4, 0.5 g; MgSO4.7H20,0.5 g; dehydrated Trypticase soy broth (BBL Microbiology Systems, Cockeysville, Md.), 0.1 g; and glucose, 1 g. pH adjusted to 3 with 1 N H2S04
1068ACIDIPHILIUM MEDIUM (DSMZ Medium 269)yeshttps://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium269.pdf

culture temp

@refgrowthtypetemperaturerange
23239nogrowth47thermophilic
23239positiveoptimum35-41
23239mesophilic
1068positivegrowth28mesophilic

culture pH

@refabilitytypepH
23239positivegrowth1.9-5.9
23239nogrowth6.1

Physiology and metabolism

oxygen tolerance

  • @ref: 23239
  • oxygen tolerance: aerobe

nutrition type

  • @ref: 23239
  • type: heterotroph

spore formation

  • @ref: 23239
  • spore formation: no

halophily

@refsaltgrowthtested relationconcentration
23239NaClnogrowth7 %
23239NaClpositivegrowth3 %

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
2323929985L-glutamate-growth
2323915356cysteine-growth
2323915428glycine-growth
2323924996lactate-growth
2323916094thiosulfate-growth
2323922599arabinose+growth
2323917234glucose+growth
2323917754glycerol+growth
2323917196L-asparagine+growth
2323930924L-tartrate+growth
2323917716lactose+growth
2323917306maltose+growth
2323929864mannitol+growth
2323916634raffinose+growth
2323933942ribose+growth
2323917992sucrose+growth

antibiotic resistance

@refChEBImetaboliteis antibioticis resistantis sensitive
2323948923erythromycinyesyes
232397507neomycinyesyes
2323917334penicillinyesyes
2323917076streptomycinyesyes
2323917698chloramphenicolyesyes
2323927644chlortetracyclinyesyes

enzymes

@refvalueactivityec
23239catalase+/-1.11.1.6
23239cytochrome oxidase+/-1.9.3.1

Isolation, sampling and environmental information

isolation

  • @ref: 1068
  • sample type: coal mine water
  • geographic location: Pennsylvania
  • country: USA
  • origin.country: USA
  • continent: North America

isolation source categories

Cat1Cat2Cat3
#Engineered#Other#Mine
#Environmental#Aquatic
#Environmental#Terrestrial#Geologic

Safety information

risk assessment

  • @ref: 1068
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Acidiphilium cryptum 16S rRNA sequenceM79366315ena524
20218Acidiphilium cryptum 16S rRNA sequenceM79367350ena524
20218Acidiphilium cryptum 16S rRNA sequenceM79368303ena524
20218Acidiphilium cryptum 16S rRNA gene, partial, strain DSM 2389Y184451337ena524
1068Acidiphilium cryptum gene for 16S ribosomal RNAD307731413ena524

GC content

@refGC-contentmethod
2323969.80thermal denaturation, midpoint method (Tm)
2323969.40Buoyant density centrifugation (BD)
106869.8

External links

@ref: 1068

culture collection no.: DSM 2389, ATCC 33463

straininfo link

@refpassport
20218http://www.straininfo.net/strains/157968
20218http://www.straininfo.net/strains/157969

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
1068Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 2389)https://www.dsmz.de/collection/catalogue/details/culture/DSM-2389
20215D.Gleim, M.Kracht, N.Weiss et. al.http://www.dsmz.de/bacterial-diversity/prokaryotic-nomenclature-up-to-date.htmlProkaryotic Nomenclature Up-to-date - compilation of all names of Bacteria and Archaea, validly published according to the Bacteriological Code since 1. Jan. 1980, and validly published nomenclatural changes since
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
23239Arthur P., Harrison JR.10.1099/00207713-31-3-327Acidiphilium cryptum gen. nov., sp. nov., Heterotrophic Bacterium From Acidic Mineral EnvironmentsIJSEM 31: 327-332 1981