Name: Acidiphilium cryptum Lhet2
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 26

Type strain:

Species: Acidiphilium cryptum

Strain Designation: Lhet2

Culture col. no.: DSM 2389, ATCC 33463, JCM 21277, IAM 14719, IFO 14242, NBRC 14242, NCIMB 11690

Strain history: IAM 14719 <-- IFO 14242 <-- A. P. Harrison, Jr. strain Lhet2.

NCBI tax ID(s): 524 (species)

SI-ID 47606

DSM 2389, ATCC 33463, NCIMB 11690, IFO14242, IAM 14719, LMD 82.2, NCCB 82002, NBRC 14242, JCM 21277

Other strains from Acidiphilium cryptum

A. cryptum KG-4, KG-4p, DSM 2390
A. cryptum 44het, AP-44, DSM 2613
A. cryptum N-Het2, DSM 9467

Section

Acidiphilium cryptum Lhet2 is an aerobe, heterotroph, thermophilic bacterium that was isolated from coal mine water.

Gram-negative
motile
rod-shaped
aerobe
heterotroph
thermophilic
16S sequence
Bacteria

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Pseudomonadota*
	Class	*Alphaproteobacteria*
	Order	*Rhodospirillales*
	Family	*Acetobacteraceae*
	Genus	*Acidiphilium*
	Species	**Acidiphilium cryptum**
	Full Scientific Name (LPSN)	Acidiphilium cryptum Harrison 1981

Information on morphological and physiological properties Morphology

[Ref.: #23239]	Gram stain	negative
[Ref.: #23239]	Gram stain	negative

[Ref.: #23239]	Cell length	0.6-0.8 µm
[Ref.: #23239]	Cell length	0.6-0.8 µm

[Ref.: #23239]	Cell width	0.3-0.4 µm
[Ref.: #23239]	Cell width	0.3-0.4 µm

[Ref.: #23239]	Cell shape	rod-shaped
[Ref.: #23239]	Cell shape	rod-shaped

[Ref.: #23239]	Motility	yes
[Ref.: #23239]	Motility	yes

[Ref.: #23239]	Flagellum arrangement	monotrichous, polar
[Ref.: #23239]	Flagellum arrangement	lophotrichous

[Ref.: #23239]	Colony size	0.2-0.5 mm
[Ref.: #23239]	Incubation period	5-7 days
[Ref.: #23239]	Cultivation medium used	TS-glucose

[Ref.: #23239]	Colony color	translucent
[Ref.: #23239]	Incubation period	2-3 days
[Ref.: #23239]	Cultivation medium used	TS-glucose

[Ref.: #23239]	Colony color	opaque, white or pinkish white
[Ref.: #23239]	Cultivation medium used	TS-glucose

Information on culture and growth conditions Culture and growth conditions

[Ref.: #23239]

Culture medium

TS-glucose

[Ref.: #23239]

Culture medium growth

[Ref.: #23239]

Culture medium composition

expand / minimize

[Ref.: #1068]

Culture medium

ACIDIPHILIUM MEDIUM (DSMZ Medium 269)

[Ref.: #1068]

Culture medium growth

[Ref.: #1068]

Culture medium link

Medium recipe at MediaDive

[Ref.: #1068]

Culture medium composition

expand / minimize

	Kind of temperature		Temperature
[Ref.: #1068]		growth	28 °C
[Ref.: #23239]	no	growth	47 °C
[Ref.: #23239]		optimum	35-41 °C
[Ref.: #67770]		growth	27 °C

[Ref.: #1068]	Temperature range	mesophilic
[Ref.: #23239]	Temperature range	thermophilic
[Ref.: #23239]	Temperature range	mesophilic
[Ref.: #67770]	Temperature range	mesophilic

	pH	Kind of pH		pH
[Ref.: #23239]			growth	1.9-5.9
[Ref.: #23239]		no	growth	6.1

Information on physiology and metabolism Physiology and metabolism

[Ref.: #23239]

Oxygen tolerance

aerobe

[Ref.: #23239]

Nutrition type

heterotroph

[Ref.: #23239]

Ability of spore formation

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #23239]		NaCl	no	growth	7	%
[Ref.: #23239]		NaCl		growth	3	%

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #23239]	arabinose ChEBI	+	growth
[Ref.: #23239]	cysteine ChEBI	-	growth
[Ref.: #23239]	glucose ChEBI	+	growth
[Ref.: #23239]	glycerol ChEBI	+	growth
[Ref.: #23239]	glycine ChEBI	-	growth
[Ref.: #23239]	L-asparagine ChEBI	+	growth
[Ref.: #23239]	L-glutamate ChEBI	-	growth
[Ref.: #23239]	L-tartrate ChEBI	+	growth
[Ref.: #23239]	lactate ChEBI	-	growth
[Ref.: #23239]	lactose ChEBI	+	growth
[Ref.: #23239]	maltose ChEBI	+	growth
[Ref.: #23239]	mannitol ChEBI	+	growth
[Ref.: #23239]	raffinose ChEBI	+	growth
[Ref.: #23239]	ribose ChEBI	+	growth
[Ref.: #23239]	sucrose ChEBI	+	growth
[Ref.: #23239]	thiosulfate ChEBI	-	growth
	Only first 10 entries are displayed. Click here to see all.

	Metabolite	Antibiotic sensitivity	Antibiotic resistance
[Ref.: #23239]	chloramphenicol ChEBI	yes
[Ref.: #23239]	chlortetracyclin ChEBI	yes
[Ref.: #23239]	erythromycin ChEBI		yes
[Ref.: #23239]	neomycin ChEBI		yes
[Ref.: #23239]	penicillin ChEBI		yes
[Ref.: #23239]	streptomycin ChEBI		yes

	Enzymes	Enzyme	Enzyme activity	EC number
[Ref.: #23239]		catalase	+/-	1.11.1.6
[Ref.: #23239]		cytochrome oxidase	+/-	1.9.3.1

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #1068]	Sample type/isolated from	coal mine water
[Ref.: #1068]	Geographic location (country and/or sea, region)	Pennsylvania
[Ref.: #1068]	Country	USA
[Ref.: #1068]	Country ISO 3 Code	USA
[Ref.: #1068]	Continent	North America

[Ref.: #67770]	Sample type/isolated from	Culture of Thiobacillus ferrooxidans, originally grown by W. W. Leathen from coal mine water
[Ref.: #67770]	Host species	Thiobacillus ferrooxidans
[Ref.: #67770]	Geographic location (country and/or sea, region)	PA
[Ref.: #67770]	Country	USA
[Ref.: #67770]	Country ISO 3 Code	USA
[Ref.: #67770]	Continent	North America
* marker position based on {}

Isolation sources categories

#Engineered	#Other	#Mine
#Environmental	#Aquatic	-
#Environmental	#Terrestrial	#Geologic

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence D30773 (>99% sequence identity) for Acidiphilium from Microbeatlas

Aquatic Samples	1984
Soil Samples	1654
Animal Samples	607
Plant Samples	360
Total Samples	4605

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #1068]

Biosafety level

Risk group (German classification)
According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #1068]	Acidiphilium cryptum gene for 16S ribosomal RNA	D30773	1413	ENA	524 tax ID
[Ref.: #20218]	Acidiphilium cryptum 16S rRNA gene, partial, strain DSM 2389	Y18445	1337	ENA	524 tax ID	*
[Ref.: #20218]	Acidiphilium cryptum 16S rRNA sequence	M79366	315	ENA	524 tax ID	*
[Ref.: #20218]	Acidiphilium cryptum 16S rRNA sequence	M79367	350	ENA	524 tax ID	*
[Ref.: #20218]	Acidiphilium cryptum 16S rRNA sequence	M79368	303	ENA	524 tax ID	*

[Ref.: #23239]	GC-content	69.80 mol%	thermal denaturation, midpoint method (Tm)
[Ref.: #23239]	GC-content	69.40 mol%	Buoyant density centrifugation (BD)
[Ref.: #1068]	GC-content	69.8 mol%

Availability in culture collections External links

[Ref.: #1068]

Culture collection no.

DSM 2389, ATCC 33463, JCM 21277, IAM 14719, IFO 14242, NBRC 14242, NCIMB 11690

[Ref.: #69710]

SI-ID 47606

Literature:

Topic	Title	Authors	Journal	DOI	Year
Metabolism	Boosting Escherichia coli's heterologous production rate of ectoines by exploiting the non-halophilic gene cluster from Acidiphilium cryptum.	Bethlehem L, Moritz KD	Extremophiles	10.1007/s00792-020-01188-8	2020	*
Metabolism	The hydroxyectoine gene cluster of the non-halophilic acidophile Acidiphilium cryptum.	Moritz KD, Amendt B, Witt EM, Galinski EA	Extremophiles	10.1007/s00792-014-0687-0	2014	*
Metabolism	Iron respiration by Acidiphilium cryptum at pH 5.	Bilgin AA, Silverstein J, Jenkins JD	FEMS Microbiol Ecol	10.1016/j.femsec.2003.08.018	2004	*
Metabolism	Glucose catabolism in strains of acidophilic, heterotrophic bacteria.	Shuttleworth KL, Unz RF, Wichlacz PL	Appl Environ Microbiol	10.1128/aem.50.3.573-579.1985	1985	*

References

#1068

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 2389

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#20218

Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )

#23239

Arthur P., Harrison JR.: Acidiphilium cryptum gen. nov., sp. nov., Heterotrophic Bacterium From Acidic Mineral Environments. IJSEM 31: 327 - 332 1981 ( DOI 10.1099/00207713-31-3-327 )

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69710

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID47606.1 )

* These data were automatically processed and therefore are not curated

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