Strain identifier

BacDive ID: 2573

Type strain: No

Species: Clostridium cochlearium

Strain Designation: 998

Strain history: ATCC 17794 <-- L. S. McClung 998 <-- S. E. Hartsell M-10.

NCBI tax ID(s): 1494 (species)

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General

@ref: 981

BacDive-ID: 2573

DSM-Number: 2153

keywords: genome sequence, 16S sequence, Bacteria, anaerobe, spore-forming, mesophilic, Gram-positive, human pathogen

description: Clostridium cochlearium 998 is an anaerobe, spore-forming, mesophilic human pathogen of the family Clostridiaceae.

NCBI tax id

  • NCBI tax id: 1494
  • Matching level: species

strain history

@refhistory
981<- ATCC <- L. S. McClung, University of Wisconsin, Madison, USA; 998 (Clostridium lentoputrescens)
67770ATCC 17794 <-- L. S. McClung 998 <-- S. E. Hartsell M-10.

doi: 10.13145/bacdive2573.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Clostridia
  • order: Eubacteriales
  • family: Clostridiaceae
  • genus: Clostridium
  • species: Clostridium cochlearium
  • full scientific name: Clostridium cochlearium (Douglas et al. 1919) Bergey et al. 1923 (Approved Lists 1980)
  • synonyms

    @refsynonym
    20215Clostridium lentoputrescens
    20215Bacillus cochlearius

@ref: 981

domain: Bacteria

phylum: Firmicutes

class: Clostridia

order: Clostridiales

family: Clostridiaceae

genus: Clostridium

species: Clostridium cochlearium

full scientific name: Clostridium cochlearium (Douglas et al. 1919) Bergey et al. 1923

strain designation: 998

type strain: no

Morphology

cell morphology

  • @ref: 69480
  • gram stain: positive
  • confidence: 100

Culture and growth conditions

culture medium

  • @ref: 981
  • name: CHOPPED MEAT MEDIUM (DSMZ Medium 78)
  • growth: yes
  • link: https://mediadive.dsmz.de/medium/78
  • composition: Name: CHOPPED MEAT MEDIUM (DSMZ Medium 78) Composition: Ground beef 500.0 g/l Casitone 30.0 g/l Agar 15.0 g/l Ethanol 9.5 g/l (optional) K2HPO4 5.0 g/l Yeast extract 5.0 g/l L-Cysteine HCl 0.5 g/l Haemin 0.005 g/l (optional) Resazurin 0.001 g/l Vitamin K3 0.0005 g/l (optional) Vitamin K1 (optional) NaOH (optional) Distilled water

culture temp

@refgrowthtypetemperaturerange
981positivegrowth37mesophilic
67770positivegrowth37mesophilic

Physiology and metabolism

oxygen tolerance

@refoxygen toleranceconfidence
981anaerobe
69480anaerobe99.229

spore formation

@refspore formationconfidence
69481yes100
69480yes100

Isolation, sampling and environmental information

taxonmaps

  • @ref: 69479
  • File name: preview.99_16844.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_34;96_643;97_746;98_865;99_16844&stattab=map
  • Last taxonomy: Clostridium cochlearium
  • 16S sequence: LC007105
  • Sequence Identity:
  • Total samples: 3721
  • soil counts: 855
  • aquatic counts: 733
  • animal counts: 1929
  • plant counts: 204

Safety information

risk assessment

  • @ref: 981
  • pathogenicity human: yes, in single cases
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

  • @ref: 67770
  • description: Clostridium cochlearium gene for 16S ribosomal RNA, partial sequence, strain: JCM 1384
  • accession: LC007105
  • length: 1474
  • database: ena
  • NCBI tax ID: 1494

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Clostridium cochlearium strain NCTC130281494.16wgspatric1494
66792Clostridium cochlearium NCTC 130282808606730draftimg1494
67770Clostridium cochlearium NCTC13028GCA_900446995contigncbi1494

GC content

@refGC-contentmethod
98127.0
6777027thermal denaturation, midpoint method (Tm)

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingyes100no
motileyes74.915no
gram-positiveyes69.118no
anaerobicyes99.335yes
aerobicno96.506no
halophileno84.535no
spore-formingyes96.796no
thermophileno80.734yes
glucose-utilyes85.1no
flagellatedno61.328no
glucose-fermentyes62.083no

External links

@ref: 981

culture collection no.: DSM 2153, ATCC 17794, NCIMB 10629, JCM 1384, BCRC 14549, NCTC 13028

straininfo link

  • @ref: 72106
  • straininfo: 36733

Reference

@idauthorscataloguedoi/urltitle
981Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 2153)https://www.dsmz.de/collection/catalogue/details/culture/DSM-2153
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch10.21203/rs.3.rs-2527258/v1Predictions based on the model GenomeNet Sporulation v. 1
72106Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID36733.1StrainInfo: A central database for resolving microbial strain identifiers