Strain identifier
BacDive ID: 2530
Type strain: 
Species: Clostridium acetobutylicum
Strain Designation: 2289, McClung, T, 2289, McClung
Strain history: IAM 19012 <-- S. Sugama <-- ATCC 4259 <-- NCTC 619 <-- A. C. Thaysen ("Bacillus butyricus").
NCBI tax ID(s): 991791 (strain), 1488 (species)
version 8.1 (current version)
General
@ref: 886
BacDive-ID: 2530
DSM-Number: 1731
keywords: genome sequence, 16S sequence, Bacteria, anaerobe, spore-forming, mesophilic, motile, alcohol production
description: Clostridium acetobutylicum 2289 is an anaerobe, spore-forming, mesophilic bacterium that produces alcohol.
NCBI tax id
| NCBI tax id | Matching level |
|---|---|
| 991791 | strain |
| 1488 | species |
strain history
| @ref | history |
|---|---|
| 886 | <- NCIB <- NCTC (Clostridium acetonigenum) <- A. C. Thaysen, Royal Naval Cordite Factory, Bacteriological Laboratory, Holton Heath, U.K. (Bacillus butyricus) |
| 67770 | IAM 19012 <-- S. Sugama <-- ATCC 4259 <-- NCTC 619 <-- A. C. Thaysen ("Bacillus butyricus"). |
doi: 10.13145/bacdive2530.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/bacillota
- domain: Bacteria
- phylum: Bacillota
- class: Clostridia
- order: Eubacteriales
- family: Clostridiaceae
- genus: Clostridium
- species: Clostridium acetobutylicum
- full scientific name: Clostridium acetobutylicum McCoy et al. 1926 (Approved Lists 1980)
@ref: 886
domain: Bacteria
phylum: Firmicutes
class: Clostridia
order: Clostridiales
family: Clostridiaceae
genus: Clostridium
species: Clostridium acetobutylicum
full scientific name: Clostridium acetobutylicum McCoy et al. 1926 emend. Keis et al. 2001
strain designation: 2289, McClung, T, 2289, McClung
type strain: no
Morphology
cell morphology
| @ref | motility | confidence | gram stain |
|---|---|---|---|
| 69480 | yes | 94.962 | |
| 69480 | 100 | positive |
Culture and growth conditions
culture medium
- @ref: 886
- name: CLOSTRIDIUM ACETOBUTYLICUM MEDIUM (DSMZ Medium 411)
- growth: yes
- link: https://mediadive.dsmz.de/medium/411
- composition: Name: CLOSTRIDIUM ACETOBUTYLICUM MEDIUM (DSMZ Medium 411) Composition: Potato 200.0 g/l D-Glucose 6.0 g/l CaCO3 2.0 g/l L-Cysteine HCl x H2O 0.5 g/l Sodium resazurin 0.0005 g/l Distilled water
culture temp
| @ref | growth | type | temperature | range |
|---|---|---|---|---|
| 886 | positive | growth | 37 | mesophilic |
| 67770 | positive | growth | 37 | mesophilic |
Physiology and metabolism
oxygen tolerance
| @ref | oxygen tolerance | confidence |
|---|---|---|
| 886 | anaerobe | |
| 69480 | anaerobe | 98.973 |
spore formation
| @ref | spore formation | confidence |
|---|---|---|
| 69481 | yes | 100 |
| 69480 | yes | 100 |
compound production
| @ref | compound |
|---|---|
| 886 | acetone |
| 886 | n butanol |
metabolite production
| @ref | Chebi-ID | metabolite | production |
|---|---|---|---|
| 67770 | 15347 | acetone | yes |
| 67770 | 28885 | n-butanol | yes |
Safety information
risk assessment
- @ref: 886
- biosafety level: 1
- biosafety level comment: Risk group (German classification)
Sequence information
16S sequences
| @ref | description | accession | length | database | NCBI tax ID |
|---|---|---|---|---|---|
| 20218 | C.acetobutylicum (DSM 1731) 16S rRNA | X78071 | 1455 | ena | 991791 |
| 67770 | Clostridium beijerinckii gene for 16S ribosomal RNA, partial sequence, strain: JCM 7829 | LC071788 | 1473 | ena | 1520 |
Genome sequences
| @ref | description | accession | assembly level | database | NCBI tax ID |
|---|---|---|---|---|---|
| 66792 | Clostridium acetobutylicum DSM 1731 | GCA_000218855 | complete | ncbi | 991791 |
| 66792 | Clostridium acetobutylicum DSM 1731 | 991791.12 | plasmid | patric | 991791 |
| 66792 | Clostridium acetobutylicum DSM 1731 | 991791.3 | complete | patric | 991791 |
| 66792 | Clostridium acetobutylicum DSM 1731 | 991791.11 | plasmid | patric | 991791 |
| 66792 | Clostridium acetobutylicum DSM 1731 | 650716024 | complete | img | 991791 |
Genome-based predictions
predictions
| trait | prediction | confidence | training_data |
|---|---|---|---|
| spore-forming | yes | 100 | no |
| motile | yes | 80.208 | no |
| flagellated | yes | 70.107 | no |
| gram-positive | yes | 80.136 | no |
| anaerobic | yes | 96.981 | yes |
| aerobic | no | 98.04 | yes |
| halophile | no | 95.572 | no |
| spore-forming | yes | 96.728 | no |
| thermophile | no | 97.407 | yes |
| glucose-util | yes | 90.183 | no |
| glucose-ferment | yes | 61.374 | no |
External links
@ref: 886
culture collection no.: DSM 1731, ATCC 4259, NCIMB 619, JCM 7829, IAM 19012, NCTC 619
straininfo link
- @ref: 72064
- straininfo: 264257
literature
| topic | Pubmed-ID | title | authors | journal | DOI | year | mesh | topic2 |
|---|---|---|---|---|---|---|---|---|
| Metabolism | 1548233 | Replacement of the aliphatic chains of Clostridium acetobutylicum by exogenous fatty acids: regulation of phospholipid and glycolipid composition. | Johnston NC, Goldfine H | J Bacteriol | 10.1128/jb.174.6.1848-1853.1992 | 1992 | Biotin/metabolism, Cell Membrane/metabolism, Clostridium/*metabolism, Fatty Acids/chemistry/*metabolism, Glycolipids/*metabolism, Membrane Lipids/*metabolism, Membrane Proteins/metabolism, *Oleic Acid, Oleic Acids/metabolism, Phospholipids/*metabolism, Solubility | Lipids |
| Enzymology | 1705930 | Purification and characterization of the DNA-dependent RNA polymerase from Clostridium acetobutylicum. | Pich A, Bahl H | J Bacteriol | 10.1128/jb.173.6.2120-2124.1991 | 1991 | Blotting, Western, Clostridium/*enzymology, DNA-Directed RNA Polymerases/chemistry/*isolation & purification/metabolism, Magnesium/pharmacology, Manganese/pharmacology, Molecular Weight, Osmolar Concentration, Protein Denaturation, RNA, Bacterial/biosynthesis, Templates, Genetic, Transcription, Genetic | Phylogeny |
| Enzymology | 9353928 | Acetate kinase from Clostridium acetobutylicum: a highly specific enzyme that is actively transcribed during acidogenesis and solventogenesis. | Winzer K, Lorenz K, DUrre P | Microbiology (Reading) | 10.1099/00221287-143-10-3279 | 1997 | Acetate Kinase/chemistry/*genetics/*metabolism, Acids/metabolism, Base Sequence, Cloning, Molecular, Clostridium/*enzymology/*genetics/metabolism, Dimerization, Kinetics, Molecular Weight, Oligodeoxyribonucleotides/genetics, Solvents/metabolism, Substrate Specificity, Transcription, Genetic | Metabolism |
| Metabolism | 10937486 | Acetone, butanol and ethanol production from domestic organic waste by solventogenic clostridia. | Claassen PA, Budde MA, Lopez-Contreras AM | J Mol Microbiol Biotechnol | 2000 | Acetone/*metabolism, Butanols/*metabolism, Cellulose/chemistry/metabolism, Clostridium/*metabolism, Ethanol/*metabolism, Fermentation, Hydrolysis, Monosaccharides/metabolism, Waste Management/*methods, Waste Products/analysis | Biotechnology | |
| Stress | 16347709 | Stress- and Growth Phase-Associated Proteins of Clostridium acetobutylicum. | Terracciano JS, Rapaport E, Kashket ER | Appl Environ Microbiol | 10.1128/aem.54.8.1989-1995.1988 | 1988 | ||
| Proteome | 20426490 | Proteome reference map and comparative proteomic analysis between a wild type Clostridium acetobutylicum DSM 1731 and its mutant with enhanced butanol tolerance and butanol yield. | Mao S, Luo Y, Zhang T, Li J, Bao G, Zhu Y, Chen Z, Zhang Y, Li Y, Ma Y | J Proteome Res | 10.1021/pr9012078 | 2010 | Bacterial Proteins/*chemistry/classification/genetics, Butanols/*metabolism/*pharmacology, Clostridium acetobutylicum/*chemistry/genetics/metabolism, Cluster Analysis, Cytoplasm, Drug Resistance, Electrophoresis, Gel, Two-Dimensional, Fermentation, Gene Expression Regulation, Bacterial, Mass Spectrometry, Metabolic Networks and Pathways, Mutation, Proteome/chemistry/genetics, Proteomics/*methods, Reverse Transcriptase Polymerase Chain Reaction, Stress, Physiological | Metabolism |
| Metabolism | 21216898 | Formic acid triggers the "Acid Crash" of acetone-butanol-ethanol fermentation by Clostridium acetobutylicum. | Wang S, Zhang Y, Dong H, Mao S, Zhu Y, Wang R, Luan G, Li Y | Appl Environ Microbiol | 10.1128/AEM.01835-10 | 2011 | Acetic Acid/toxicity, Acetone/*metabolism, Butanols/*metabolism, Butyric Acid/toxicity, Clostridium acetobutylicum/drug effects/*growth & development/*metabolism, Culture Media/chemistry, Ethanol/*metabolism, Fermentation, Formates/*toxicity, Glucose/metabolism, Hydrogen-Ion Concentration, Plasmids, Zea mays/metabolism | Pathogenicity |
| Metabolism | 21296183 | Engineering the robustness of Clostridium acetobutylicum by introducing glutathione biosynthetic capability. | Zhu L, Dong H, Zhang Y, Li Y | Metab Eng | 10.1016/j.ymben.2011.01.009 | 2011 | Butanols/*metabolism, *Clostridium acetobutylicum/enzymology/genetics/growth & development, Escherichia coli/enzymology/genetics, Escherichia coli Proteins/biosynthesis/genetics, *Genetic Engineering, *Glutathione/biosynthesis/genetics, *Organisms, Genetically Modified/genetics/growth & development/metabolism | Enzymology |
| Enzymology | 21384033 | Comparative analysis on the membrane proteome of Clostridium acetobutylicum wild type strain and its butanol-tolerant mutant. | Mao S, Luo Y, Bao G, Zhang Y, Li Y, Ma Y | Mol Biosyst | 10.1039/c0mb00330a | 2011 | Adaptation, Physiological/drug effects/genetics, Bacterial Proteins/*analysis/classification, Butanols/pharmacology, Chromatography, Liquid, Clostridium acetobutylicum/genetics/*metabolism, Cluster Analysis, Electrophoresis, Gel, Two-Dimensional, Electrophoresis, Polyacrylamide Gel, Mass Spectrometry, Membrane Proteins/*analysis/classification, Mutation, Proteome/*analysis/classification, Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization | Phylogeny |
| Genetics | 21742891 | Complete genome sequence of Clostridium acetobutylicum DSM 1731, a solvent-producing strain with multireplicon genome architecture. | Bao G, Wang R, Zhu Y, Dong H, Mao S, Zhang Y, Chen Z, Li Y, Ma Y | J Bacteriol | 10.1128/JB.05596-11 | 2011 | Clostridium acetobutylicum/*genetics/metabolism, DNA, Bacterial/*chemistry/*genetics, *Genome, Bacterial, Molecular Sequence Data, Mutagenesis, Insertional, Plasmids, *Sequence Analysis, DNA, Solvents/metabolism | Metabolism |
| Biotechnology | 22144358 | Use of proteomic tools in microbial engineering for biofuel production. | Mao S, Jia K, Zhang Y, Li Y | Methods Mol Biol | 10.1007/978-1-61779-483-4_10 | 2012 | Bacterial Proteins/genetics/metabolism, *Biofuels, Clostridium acetobutylicum/genetics/metabolism, Cluster Analysis, Electrophoresis, Gel, Two-Dimensional, Fermentation, Mass Spectrometry, Metabolic Engineering/*methods, Proteome, Proteomics/*methods | Metabolism |
| Metabolism | 22306328 | Novel and neglected issues of acetone-butanol-ethanol (ABE) fermentation by clostridia: Clostridium metabolic diversity, tools for process mapping and continuous fermentation systems. | Patakova P, Linhova M, Rychtera M, Paulova L, Melzoch K | Biotechnol Adv | 10.1016/j.biotechadv.2012.01.010 | 2012 | Acetone/*metabolism, Butanols/*metabolism, Clostridium/cytology/genetics/*metabolism, Ethanol/*metabolism, *Fermentation, Flow Cytometry, Glycerol/metabolism, Hexoses/metabolism, Inulin/metabolism, Lactose/metabolism, Oligonucleotide Array Sequence Analysis, Pentoses/metabolism, Spectroscopy, Fourier Transform Infrared, Spectrum Analysis, Raman | Transcriptome |
| Enzymology | 22742819 | Introducing a single secondary alcohol dehydrogenase into butanol-tolerant Clostridium acetobutylicum Rh8 switches ABE fermentation to high level IBE fermentation. | Dai Z, Dong H, Zhu Y, Zhang Y, Li Y, Ma Y | Biotechnol Biofuels | 10.1186/1754-6834-5-44 | 2012 | ||
| Metabolism | 23702687 | Discovery of a novel gene involved in autolysis of Clostridium cells. | Yang L, Bao G, Zhu Y, Dong H, Zhang Y, Li Y | Protein Cell | 10.1007/s13238-013-3025-x | 2013 | Autolysis/genetics, Clostridium acetobutylicum/*genetics/metabolism, Computational Biology, *Genes, Bacterial, N-Acetylmuramoyl-L-alanine Amidase/genetics/*metabolism, Temperature | |
| Metabolism | 23933028 | Felled oil palm trunk as a renewable source for biobutanol production by Clostridium spp. | Komonkiat I, Cheirsilp B | Bioresour Technol | 10.1016/j.biortech.2013.07.067 | 2013 | *Biofuels, Bioreactors, Butanols/*chemistry, Carbohydrates/chemistry, Clostridium/*metabolism, Clostridium acetobutylicum/*metabolism, Clostridium beijerinckii/*metabolism, Fermentation, Hydrolysis, Kinetics, Palm Oil, Plant Oils/*chemistry, Species Specificity, Time Factors, Trees/*chemistry | Enzymology |
| Phylogeny | 24600866 | Evaluation of hydrogen production by clostridium strains on beet molasses. | Avci A, Kilic NK, Donmez G, Donmez S | Environ Technol | 10.1080/09593330.2013.826251 | 2014 | Beta vulgaris/*microbiology, Biofuels/*microbiology, Clostridium/*classification/isolation & purification/*physiology, Hydrogen/*isolation & purification/*metabolism, Molasses/*microbiology, Species Specificity | Enzymology |
| Proteome | 25044112 | Comparative genomic and proteomic analyses of Clostridium acetobutylicum Rh8 and its parent strain DSM 1731 revealed new understandings on butanol tolerance. | Bao G, Dong H, Zhu Y, Mao S, Zhang T, Zhang Y, Chen Z, Li Y | Biochem Biophys Res Commun | 10.1016/j.bbrc.2014.07.052 | 2014 | Butanols/*pharmacology, Clostridium acetobutylicum/drug effects/genetics/*metabolism, Genes, Bacterial, Mutation, *Proteome | Pathogenicity |
| Metabolism | 34325704 | Enforcing ATP hydrolysis enhanced anaerobic glycolysis and promoted solvent production in Clostridium acetobutylicum. | Dai Z, Zhu Y, Dong H, Zhao C, Zhang Y, Li Y | Microb Cell Fact | 10.1186/s12934-021-01639-7 | 2021 | Acetone/metabolism, Adenosine Triphosphate/*metabolism, Anaerobiosis, Biofuels, Butanols/metabolism, Clostridium acetobutylicum/genetics/*metabolism, Ethanol/metabolism, *Fermentation, *Glycolysis, Hydrolysis, Solvents/*metabolism | Biotechnology |
Reference
| @id | authors | catalogue | doi/url | title | journal | pubmed |
|---|---|---|---|---|---|---|
| 886 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 1731) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-1731 | |||
| 20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | |||
| 20218 | Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P. | 10.1016/j.syapm.2013.11.002 | StrainInfo introduces electronic passports for microorganisms. | Syst Appl Microbiol. 37: 42-50 2014 | 24321274 | |
| 66792 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | |||
| 67770 | Curators of the JCM | https://jcm.brc.riken.jp/en/ | ||||
| 69480 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | |||
| 69481 | Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch | 10.21203/rs.3.rs-2527258/v1 | Predictions based on the model GenomeNet Sporulation v. 1 | |||
| 72064 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID264257.1 | StrainInfo: A central database for resolving microbial strain identifiers |