Strain identifier
BacDive ID: 2528
Type strain: 
Species: Clostridium aceticum
Strain history: DSM 1496 <-- M. Braun <-- H. A. Barker <-- K. T. Wieringa.
NCBI tax ID(s): 84022 (species)
version 8.1 (current version)
General
@ref: 793
BacDive-ID: 2528
DSM-Number: 1496
keywords: genome sequence, 16S sequence, Bacteria, anaerobe, spore-forming, mesophilic, motile
description: Clostridium aceticum DSM 1496 is an anaerobe, spore-forming, mesophilic bacterium that was isolated from mud.
NCBI tax id
- NCBI tax id: 84022
- Matching level: species
strain history
| @ref | history |
|---|---|
| 793 | <- M. Braun, Universität Göttingen, Germany <- H. A. Barker, University of California, Berkeley, USA <- K. T. Wieringa, Agricultural University Wageningen, Laboratory of Microbiology, Netherlands |
| 67770 | DSM 1496 <-- M. Braun <-- H. A. Barker <-- K. T. Wieringa. |
doi: 10.13145/bacdive2528.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/bacillota
- domain: Bacteria
- phylum: Bacillota
- class: Clostridia
- order: Eubacteriales
- family: Clostridiaceae
- genus: Clostridium
- species: Clostridium aceticum
- full scientific name: Clostridium aceticum (ex Wieringa 1940) Gottschalk and Braun 1981
@ref: 793
domain: Bacteria
phylum: Firmicutes
class: Clostridia
order: Clostridiales
family: Clostridiaceae
genus: Clostridium
species: Clostridium aceticum
full scientific name: Clostridium aceticum (ex Wieringa 1940) Gottschalk and Braun 1981
type strain: yes
Morphology
cell morphology
| @ref | motility | confidence | gram stain |
|---|---|---|---|
| 69480 | yes | 93.474 | |
| 69480 | 98.866 | positive |
Culture and growth conditions
culture medium
- @ref: 793
- name: ACETOBACTERIUM MEDIUM (DSMZ Medium 135)
- growth: yes
- link: https://mediadive.dsmz.de/medium/135
- composition: Name: ACETOBACTERIUM MEDIUM (DSMZ Medium 135) Composition: NaHCO3 9.79432 g/l D-Fructose 9.79432 g/l Yeast extract 1.95886 g/l NH4Cl 0.979432 g/l Na2S x 9 H2O 0.489716 g/l L-Cysteine HCl x H2O 0.489716 g/l K2HPO4 0.440744 g/l KH2PO4 0.323213 g/l MgSO4 x 7 H2O 0.0587659 g/l Nitrilotriacetic acid 0.029383 g/l NaCl 0.0195886 g/l MnSO4 x H2O 0.00979432 g/l ZnSO4 x 7 H2O 0.00352595 g/l CoSO4 x 7 H2O 0.00352595 g/l FeSO4 x 7 H2O 0.00195886 g/l CaCl2 x 2 H2O 0.00195886 g/l NiCl2 x 6 H2O 0.000587659 g/l Sodium resazurin 0.000489716 g/l AlK(SO4)2 x 12 H2O 0.000391773 g/l CuSO4 x 5 H2O 0.000195886 g/l H3BO3 0.000195886 g/l Na2MoO4 x 2 H2O 0.000195886 g/l Pyridoxine hydrochloride 9.79432e-05 g/l Calcium D-(+)-pantothenate 4.89716e-05 g/l Nicotinic acid 4.89716e-05 g/l Riboflavin 4.89716e-05 g/l (DL)-alpha-Lipoic acid 4.89716e-05 g/l p-Aminobenzoic acid 4.89716e-05 g/l Thiamine HCl 4.89716e-05 g/l Folic acid 1.95886e-05 g/l Biotin 1.95886e-05 g/l Na2WO4 x 2 H2O 7.83546e-06 g/l Na2SeO3 x 5 H2O 5.87659e-06 g/l Vitamin B12 9.79432e-07 g/l Distilled water
culture temp
| @ref | growth | type | temperature | range |
|---|---|---|---|---|
| 793 | positive | growth | 30 | mesophilic |
| 67770 | positive | growth | 30 | mesophilic |
Physiology and metabolism
oxygen tolerance
| @ref | oxygen tolerance | confidence |
|---|---|---|
| 793 | anaerobe | |
| 69480 | anaerobe | 99.718 |
spore formation
| @ref | spore formation | confidence |
|---|---|---|
| 69481 | yes | 100 |
| 69480 | yes | 100 |
Isolation, sampling and environmental information
isolation
| @ref | sample type |
|---|---|
| 793 | mud |
| 67770 | Mud |
isolation source categories
- Cat1: #Environmental
- Cat2: #Terrestrial
- Cat3: #Mud (Sludge)
Safety information
risk assessment
- @ref: 793
- biosafety level: 1
- biosafety level comment: Risk group (German classification)
Sequence information
16S sequences
| @ref | description | accession | length | database | NCBI tax ID |
|---|---|---|---|---|---|
| 793 | Clostridium aceticum 16S rRNA gene, partial, strain DSM 1496 | Y18183 | 1368 | ena | 84022 |
| 67770 | Clostridium aceticum gene for 16S ribosomal RNA, partial sequence | AB910752 | 1283 | ena | 84022 |
Genome sequences
| @ref | description | accession | assembly level | database | NCBI tax ID |
|---|---|---|---|---|---|
| 66792 | Clostridium aceticum DSM 1496 | GCA_001042715 | complete | ncbi | 84022 |
| 66792 | Clostridium aceticum strain DSM 1496 | 84022.5 | wgs | patric | 84022 |
| 66792 | Clostridium aceticum strain DSM 1496 | 84022.6 | complete | patric | 84022 |
| 66792 | Clostridium aceticum strain DSM 1496 | 84022.13 | plasmid | patric | 84022 |
| 66792 | Clostridium aceticum, DSM 1496 | 2609459698 | complete | img | 84022 |
| 66792 | Clostridium aceticum DSM 1496 | 2630968326 | draft | img | 84022 |
| 67770 | Clostridium aceticum DSM 1496 | GCA_000949285 | scaffold | ncbi | 84022 |
GC content
| @ref | GC-content |
|---|---|
| 793 | 33.0 |
| 67770 | 33 |
Genome-based predictions
predictions
| trait | prediction | confidence | training_data |
|---|---|---|---|
| spore-forming | yes | 100 | no |
| motile | yes | 84.596 | no |
| flagellated | yes | 51.138 | no |
| gram-positive | yes | 55.68 | no |
| anaerobic | yes | 98.271 | no |
| aerobic | no | 97.174 | no |
| halophile | no | 77.854 | no |
| spore-forming | yes | 95.377 | no |
| thermophile | no | 98.574 | yes |
| glucose-util | yes | 86.146 | no |
| glucose-ferment | no | 60.062 | no |
External links
@ref: 793
culture collection no.: DSM 1496, ATCC 35044, JCM 15732, BCRC 14468, KCTC 3248, KCTC 5002, LMG 7577
straininfo link
- @ref: 72062
- straininfo: 11825
literature
| topic | Pubmed-ID | title | authors | journal | DOI | year |
|---|---|---|---|---|---|---|
| Genetics | 25931594 | Draft Genome Sequence of Clostridium aceticum DSM 1496, a Potential Butanol Producer through Syngas Fermentation. | Song Y, Hwang S, Cho BK | Genome Announc | 10.1128/genomeA.00258-15 | 2015 |
| Genetics | 26184942 | Complete Genome Sequence of Rnf- and Cytochrome-Containing Autotrophic Acetogen Clostridium aceticum DSM 1496. | Poehlein A, Bengelsdorf FR, Schiel-Bengelsdorf B, Gottschalk G, Daniel R, Durre P | Genome Announc | 10.1128/genomeA.00786-15 | 2015 |
Reference
| @id | authors | catalogue | doi/url | title | journal | pubmed |
|---|---|---|---|---|---|---|
| 793 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 1496) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-1496 | |||
| 20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | |||
| 66792 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | |||
| 66794 | Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg | 10.1093/nar/gkaa1025 | BRENDA, the ELIXIR core data resource in 2021: new developments and updates | Nucleic Acids Res. 49: D498-D508 2020 | 33211880 | |
| 67770 | Curators of the JCM | https://jcm.brc.riken.jp/en/ | ||||
| 69480 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | |||
| 69481 | Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch | 10.21203/rs.3.rs-2527258/v1 | Predictions based on the model GenomeNet Sporulation v. 1 | |||
| 72062 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID11825.1 | StrainInfo: A central database for resolving microbial strain identifiers |