Strain identifier

BacDive ID: 2520

Type strain: Yes

Species: Caloramator coolhaasii

Strain Designation: Z

Strain history: <- DSM <- CM Plugge, Wageningen Univ., Laboratory of Microbiology, Netherlands, Z

NCBI tax ID(s): 95159 (species)

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General

@ref: 4789

BacDive-ID: 2520

DSM-Number: 12679

keywords: 16S sequence, Bacteria, anaerobe, thermophilic, rod-shaped

description: Caloramator coolhaasii Z is an anaerobe, thermophilic, rod-shaped bacterium that was isolated from anaerobic thermophilic granular sludge.

NCBI tax id

  • NCBI tax id: 95159
  • Matching level: species

strain history

@refhistory
4789<- C. M. Plugge, Wageningen University, Laboratory of Microbiology, Netherlands; Z <- C. M. Plugge {1998}
67771<- DSM <- CM Plugge, Wageningen Univ., Laboratory of Microbiology, Netherlands, Z

doi: 10.13145/bacdive2520.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Clostridia
  • order: Eubacteriales
  • family: Clostridiaceae
  • genus: Caloramator
  • species: Caloramator coolhaasii
  • full scientific name: Caloramator coolhaasii Plugge et al. 2000

@ref: 4789

domain: Bacteria

phylum: Firmicutes

class: Clostridia

order: Clostridiales

family: Clostridiaceae

genus: Caloramator

species: Caloramator coolhaasii

full scientific name: Caloramator coolhaasii Plugge et al. 2000 emend. Ogg and Patel 2011

strain designation: Z

type strain: yes

Morphology

cell morphology

@refcell shapemotilitygram stain
67771rod-shapedno
67771filament-shaped
67771negative

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
4789WILKINS-CHALGREN ANAEROBE BROTH (DSMZ Medium 339)yeshttps://mediadive.dsmz.de/medium/339Name: WILKINS-CHALGREN ANAEROBE BROTH (DSMZ Medium 339) Composition: dehydrated Wilkins-Chalgren medium 33.0 g/l L-Cysteine HCl 0.3 g/l Sodium resazurin 0.0005 g/l Distilled water
4789CALORAMATOR PROTEOCLASTICUS MEDIUM (DSMZ Medium 788)yeshttps://mediadive.dsmz.de/medium/788Name: CALORAMATOR MEDIUM (DSMZ Medium 788) Composition: D-Glucose 4.98504 g/l NH4Cl 2.39282 g/l Na2CO3 1.49551 g/l Na2HPO4 x 2 H2O 0.518445 g/l KH2PO4 0.408774 g/l MgCl2 x 6 H2O 0.398804 g/l Na2S x 9 H2O 0.299103 g/l CaCl2 x 2 H2O 0.199402 g/l Yeast extract 0.199402 g/l HCl 0.00249252 g/l FeCl2 x 4 H2O 0.00149551 g/l Sodium resazurin 0.000498504 g/l NaOH 0.000498504 g/l Pyridoxine hydrochloride 0.000299103 g/l Nicotinic acid 0.000199402 g/l Thiamine-HCl x 2 H2O 0.000199402 g/l CoCl2 x 6 H2O 0.000189432 g/l MnCl2 x 4 H2O 9.97009e-05 g/l Calcium pantothenate 9.97009e-05 g/l Vitamin B12 9.97009e-05 g/l p-Aminobenzoic acid 7.97607e-05 g/l ZnCl2 6.97906e-05 g/l Na2MoO4 x 2 H2O 3.58923e-05 g/l NiCl2 x 6 H2O 2.39282e-05 g/l D-(+)-biotin 1.99402e-05 g/l H3BO3 5.98205e-06 g/l Na2WO4 x 2 H2O 3.98804e-06 g/l Na2SeO3 x 5 H2O 2.99103e-06 g/l CuCl2 x 2 H2O 1.99402e-06 g/l Distilled water

culture temp

@refgrowthtypetemperaturerange
4789positivegrowth55thermophilic
67771positivegrowth55thermophilic

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
4789anaerobe
67771anaerobe

spore formation

  • @ref: 67771
  • spore formation: no

Isolation, sampling and environmental information

isolation

@refsample typecountryorigin.countrycontinent
4789anaerobic thermophilic granular sludgeNetherlandsNLDEurope
67771From anaerobic thermophilic granular sludgeNetherlandsNLDEurope

isolation source categories

Cat1Cat2Cat3
#Engineered#Waste#Activated sludge
#Condition#Anoxic (anaerobic)
#Condition#Thermophilic (>45°C)

taxonmaps

  • @ref: 69479
  • File name: preview.99_13636.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_285;96_2427;97_2956;98_3697;99_13636&stattab=map
  • Last taxonomy: Thermobrachium
  • 16S sequence: NR_024955
  • Sequence Identity:
  • Total samples: 171
  • soil counts: 26
  • aquatic counts: 133
  • animal counts: 8
  • plant counts: 4

Safety information

risk assessment

  • @ref: 4789
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
4789Caloramator coolhaasii 16S ribosomal RNA gene, partial sequenceAF1042151454ena95159
67771Caloramator coolhaasii strain Z 16S ribosomal RNA, partial sequenceNR_0249551454nuccore95159

GC content

  • @ref: 4789
  • GC-content: 31.7
  • method: high performance liquid chromatography (HPLC)

External links

@ref: 4789

culture collection no.: DSM 12679, KCTC 15121

straininfo link

  • @ref: 72055
  • straininfo: 49340

literature

  • topic: Phylogeny
  • Pubmed-ID: 12361295
  • title: Development of fluorescent adjacent hybridization probes and their application in real-time PCR for the simultaneous detection and identification of Fervidobacterium and Caloramator.
  • authors: Connolly GR, Patel BKC
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/00207713-52-5-1837
  • year: 2002
  • mesh: Bacteria, Anaerobic/classification/*genetics/isolation & purification, Base Sequence, Carbocyanines, DNA Primers, DNA, Bacterial/genetics, DNA, Ribosomal/genetics, Fluorescein-5-isothiocyanate, *Fluorescent Dyes, Molecular Probe Techniques, Molecular Sequence Data, Nucleic Acid Hybridization, *Oligonucleotide Probes, Polymerase Chain Reaction, RNA, Bacterial/genetics, RNA, Ribosomal, 16S/genetics, Sequence Homology, Nucleic Acid, Species Specificity
  • topic2: Genetics

Reference

@idauthorscataloguedoi/urltitle
4789Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 12679)https://www.dsmz.de/collection/catalogue/details/culture/DSM-12679
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
67771Curators of the KCTChttps://kctc.kribb.re.kr/En/Kctc
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
72055Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID49340.1StrainInfo: A central database for resolving microbial strain identifiers